Specific primers design based on the superoxide dismutase b gene for Trypanosoma cruzi as a screening tool: Validation method using strains from Colombia classified according to their discrete typing unit

ABSTRACT: Objective: To classify 21 new isolates of Trypanosoma cruzi (T. cruzi) according to the Discrete Typing Unit (DTU) which they belong to, as well as tune up a new pair of primers designed to detect the parasite in biological samples. Methods: Strains were isolated, DNA extracted, and classified by using three Polymerase Chain Reactions (PCR). Subsequently this DNA was used along with other isolates of various biological samples, for a new PCR using primers designed. Finally, the amplified fragments were sequenced. Results: It was observed the predominance of DTU I in Colombia, as well as the specificity of our primers for detection of T. cruzi, while no band was obtained when other species were used. Conclusions: This work reveals the genetic variability of 21 new isolates of T. cruzi in Colombia.Our primers confirmed their specificity for detecting the presence of T. cruzi.

• Autores: Mejía Jaramillo, Ana María; Olmo, Francisco; Escobedo Ortegón, Javier; Palma, Patricia; Sánchez Moreno, Manuel; Triana Chávez, Omar; Clotilde, Marín
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2014
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/35212
• Acceso en línea: https://hdl.handle.net/10495/35212
• Palabra clave: Trypanosoma cruzi; Reacción en Cadena de la Polimerasa; Polymerase Chain Reaction; Colombia; Superóxido Dismutasa; Superoxide Dismutase
• Rights: openAccess
• License: https://creativecommons.org/licenses/by-nc-nd/4.0/