Caracterización de las regiones organizadoras nucleolares coloreadas con plata (AgNORs) en tumores cutáneos caninos

ABSTRACT: A silver stain technique is used to identify the Nucleolar Organizer Regions (AgNORs). Nowadays, the quantitative and qualitative evaluation of these regions is a marker to the proliferative activity in tumor cells. The purpose of this study was to characterize AgNORs in some kind of canine skin tumors. There where evaluated 28 mast cells tumors, 18 squamous cells tumors and seven basal cells tumors from the animal pathology archive and from the veterinarian clinic of the University of Antioquia as well as other locals clinic in the city of Medellín, Colombia. The samples were embedded in paraffin and cutted to four microns and stained with Hematoxylin- Eosin to check the diagnostic and Giemsa stained to classify the mast cells tumors. To evaluate morphometrically the AgNORs in the automatic image analyzer (AIA) other series of these samples were stained with silver nitrate. The following parameters were evaluated: nuclear area, AgNORs area/cell, AgNORs number/cell, and AgNORs distribution in the cell. The study of the variation from the innestability of the average values was used in direct relation with sample size in order to determinate the number of cells to be evaluated; yielding a total of 20 cells in each case for the mast cell tumors and for the squamous cell tumors, moreover a total of 30 cells in each case for the basal cell tumors. The data was statistically anallyzed using the variance analysis and by comparing the medians by the Fischer (F) test, revealing significant level of p<0.05 value. The AgNORs stain is viable in the evaluated tumors for what 30 minutes were needed for the incubation period. The statistical significant analysis (p<0.05) shows that the grade II mast cell tumors have a smaller nuclear area. Furthermore it shows significant difference (p>0.05) in the number and the area of the AgNORs between the three histopathological levels of this tumor. The stained AgNORs revealed two cellular layers in the mast cell tumors that differ from the grade classification that is used today.

• Autores: Giraldo Mejía, Gloria Eugenia; Aranzazu Taborda, Diego Alfonso; Rodríguez, Berardo de Jesús; Pérez, Mónica M.; Ramírez Rojas, María Consuelo
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2003
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: spa
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/7280
• Acceso en línea: http://hdl.handle.net/10495/7280
• Palabra clave: Neoplasias cutáneas; Neoplasias; Caninos
• Rights: openAccess
• License: Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)