Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913)

ABSTRACT: Spermatozoa in Characid fish remain immobile in seminal plasma and are activated when freed into water where the ionic balance seems to be the main factor starting the activation process. This process was the target of the present study with emphasis on the activation of motility and on mo...

Full description

Autores:
Tabares Serna, Carlos Javier
Ruiz Cortés, Zulma Tatiana
Arboleda, Lucy
Olivera Ángel, Martha
Tipo de recurso:
Article of investigation
Fecha de publicación:
2007
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/22955
Acceso en línea:
http://hdl.handle.net/10495/22955
https://revistas.unal.edu.co/index.php/actabiol/article/view/27173
Palabra clave:
Membrane Potentials
Potenciales de la Membrana
Antagonistas del calcio
Calcium - Antagonists
Ionic balance
Equilibrio ionico
Oxonol
http://aims.fao.org/aos/agrovoc/c_3933
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-sa/2.5/co/
id UDEA2_41c516761e84c296c34e67f834f5cba7
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/22955
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913)
dc.title.alternative.spa.fl_str_mv Efecto de algunos iones sobre la activación espermática en Brycon henni (Eigenmann 1913)
title Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913)
spellingShingle Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913)
Membrane Potentials
Potenciales de la Membrana
Antagonistas del calcio
Calcium - Antagonists
Ionic balance
Equilibrio ionico
Oxonol
http://aims.fao.org/aos/agrovoc/c_3933
title_short Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913)
title_full Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913)
title_fullStr Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913)
title_full_unstemmed Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913)
title_sort Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913)
dc.creator.fl_str_mv Tabares Serna, Carlos Javier
Ruiz Cortés, Zulma Tatiana
Arboleda, Lucy
Olivera Ángel, Martha
dc.contributor.author.none.fl_str_mv Tabares Serna, Carlos Javier
Ruiz Cortés, Zulma Tatiana
Arboleda, Lucy
Olivera Ángel, Martha
dc.subject.decs.none.fl_str_mv Membrane Potentials
Potenciales de la Membrana
topic Membrane Potentials
Potenciales de la Membrana
Antagonistas del calcio
Calcium - Antagonists
Ionic balance
Equilibrio ionico
Oxonol
http://aims.fao.org/aos/agrovoc/c_3933
dc.subject.lemb.none.fl_str_mv Antagonistas del calcio
Calcium - Antagonists
dc.subject.agrovoc.none.fl_str_mv Ionic balance
Equilibrio ionico
dc.subject.proposal.spa.fl_str_mv Oxonol
dc.subject.agrovocuri.none.fl_str_mv http://aims.fao.org/aos/agrovoc/c_3933
description ABSTRACT: Spermatozoa in Characid fish remain immobile in seminal plasma and are activated when freed into water where the ionic balance seems to be the main factor starting the activation process. This process was the target of the present study with emphasis on the activation of motility and on motility maintenance over time. The effect of isosmotic solutions was analyzed taking into account the possible combinations of the following ions, Ca2, K', Mg2 and Na' as well as the effect of channel blocking agents. The parameters measured were cells with motility (%), duration of motility (s), plasma membrane potential, and the effect of channel blockers on activation time and on motility. There was an increased motility when the semen was incubated in solutions containing K' (p<0.05) compared with the control (CaNaMgK solution); the longest duration of motility was attained when the incubation was performed in solutions containing Na and Mg2 (p<O.OS). All solutions induced a change in membrane potential detected after 15 s of activation. Blocking K', Ca2, and Na channels did not alter motility but decreased the activation time (p<0.05). Potassium induced activation at all concentrations up to 105 mM, but motility was drastically decreased at concentrations higher than 140 mM (p<0.05). The conclusion is that interaction of the ionic environment with the cell membrane leads to changes in membrane potential and intracellular signalling that trigger sperm motility in Brycon henni
publishDate 2007
dc.date.issued.none.fl_str_mv 2007
dc.date.accessioned.none.fl_str_mv 2021-10-06T21:13:50Z
dc.date.available.none.fl_str_mv 2021-10-06T21:13:50Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.type.local.spa.fl_str_mv Artículo de investigación
format http://purl.org/coar/resource_type/c_2df8fbb1
status_str publishedVersion
dc.identifier.citation.spa.fl_str_mv Tabares, J., Ruíz, T., Arboleda, L., & Olivera, M. (2007). Efecto de algunos iones sobre la activación espermática en Brycon henni (Eigenmann 1913). Acta Biológica Colombiana, 12(1), 87-97. Recuperado a partir de https://revistas.unal.edu.co/index.php/actabiol/article/view/27173
dc.identifier.issn.none.fl_str_mv 0120-548X
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/22955
dc.identifier.eissn.none.fl_str_mv 1900-1649
dc.identifier.url.spa.fl_str_mv https://revistas.unal.edu.co/index.php/actabiol/article/view/27173
identifier_str_mv Tabares, J., Ruíz, T., Arboleda, L., & Olivera, M. (2007). Efecto de algunos iones sobre la activación espermática en Brycon henni (Eigenmann 1913). Acta Biológica Colombiana, 12(1), 87-97. Recuperado a partir de https://revistas.unal.edu.co/index.php/actabiol/article/view/27173
0120-548X
1900-1649
url http://hdl.handle.net/10495/22955
https://revistas.unal.edu.co/index.php/actabiol/article/view/27173
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Acta Biolo. Colomb.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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dc.format.extent.spa.fl_str_mv 11
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología
dc.publisher.group.spa.fl_str_mv Biogénesis
dc.publisher.place.spa.fl_str_mv Bogotá, Colombia
institution Universidad de Antioquia
bitstream.url.fl_str_mv https://bibliotecadigital.udea.edu.co/bitstream/10495/22955/2/license_rdf
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repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
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spelling Tabares Serna, Carlos JavierRuiz Cortés, Zulma TatianaArboleda, LucyOlivera Ángel, Martha2021-10-06T21:13:50Z2021-10-06T21:13:50Z2007Tabares, J., Ruíz, T., Arboleda, L., & Olivera, M. (2007). Efecto de algunos iones sobre la activación espermática en Brycon henni (Eigenmann 1913). Acta Biológica Colombiana, 12(1), 87-97. Recuperado a partir de https://revistas.unal.edu.co/index.php/actabiol/article/view/271730120-548Xhttp://hdl.handle.net/10495/229551900-1649https://revistas.unal.edu.co/index.php/actabiol/article/view/27173ABSTRACT: Spermatozoa in Characid fish remain immobile in seminal plasma and are activated when freed into water where the ionic balance seems to be the main factor starting the activation process. This process was the target of the present study with emphasis on the activation of motility and on motility maintenance over time. The effect of isosmotic solutions was analyzed taking into account the possible combinations of the following ions, Ca2, K', Mg2 and Na' as well as the effect of channel blocking agents. The parameters measured were cells with motility (%), duration of motility (s), plasma membrane potential, and the effect of channel blockers on activation time and on motility. There was an increased motility when the semen was incubated in solutions containing K' (p<0.05) compared with the control (CaNaMgK solution); the longest duration of motility was attained when the incubation was performed in solutions containing Na and Mg2 (p<O.OS). All solutions induced a change in membrane potential detected after 15 s of activation. Blocking K', Ca2, and Na channels did not alter motility but decreased the activation time (p<0.05). Potassium induced activation at all concentrations up to 105 mM, but motility was drastically decreased at concentrations higher than 140 mM (p<0.05). The conclusion is that interaction of the ionic environment with the cell membrane leads to changes in membrane potential and intracellular signalling that trigger sperm motility in Brycon henniRESUMEN: Los espermatozoides en los carácidos se mantienen inm6viles en plasma seminal, activándose cuando se liberan en el agua. EI objetivo de este estudio fue conocer algunos de los mecanismos que inducen la activación espermática (porcentaje de espermatozoides que adquieren movilidad y el tiempo que dura la misma). EI efecto de las soluciones isoosmóticas, se analiz6 considerando las combinaciones de los iones Ca", K', Mg2 Y Na, y el efecto de algunos bloqueadores de canales i6nicos. Se evalu6 el porcentaje y duración de la movilidad, as! como el potencial de la membrana plasmática, La motilidad espermática fue mayor cuando el semen se incubo en las soluciones que contengan K' (p<O,OS) comparado con el control positivo. La mayor duración de la movilidad se obtuvo cuando se incubaron los espermatozoides en soluciones que contengan Na' y Mg2 (p<O,OS). Todas las soluciones probadas indujeron un cambio en el potencial de membrana, detectado después de 15 segundos de Ia activaci6n. Bloqueo de los canales de K', Ca' y Na' no altere la movilidad, sino que disminuyo el tiempo de activaci6n (p<0,05). Los iones potasio indujeron la activaci6n en codas las concentraciones hasta 105 mM, pero la movilidad disminuy6 drásticamente cuando las concentraciones fueron mayores de 140 mM (p<0,05). Se concluye que la interacción del ambiente iónico con la membrana de la célula conduce a cambios en el potencial de la membrana que conllevan a señales intracelulares que desencadenan la movilidad del esperma.COL006656111application/pdfengUniversidad Nacional de Colombia, Facultad de Ciencias, Departamento de BiologíaBiogénesisBogotá, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913)Efecto de algunos iones sobre la activación espermática en Brycon henni (Eigenmann 1913)Membrane PotentialsPotenciales de la MembranaAntagonistas del calcioCalcium - AntagonistsIonic balanceEquilibrio ionicoOxonolhttp://aims.fao.org/aos/agrovoc/c_3933Acta Biolo. Colomb.Acta Biológica Colombiana8798121CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051https://bibliotecadigital.udea.edu.co/bitstream/10495/22955/2/license_rdfe2060682c9c70d4d30c83c51448f4eedMD52ORIGINALRuizTatiana_2007_EfectosIonesActivacionEspermatica.pdfRuizTatiana_2007_EfectosIonesActivacionEspermatica.pdfArtículo de investigaciónapplication/pdf3230201https://bibliotecadigital.udea.edu.co/bitstream/10495/22955/1/RuizTatiana_2007_EfectosIonesActivacionEspermatica.pdfd25744236c2bb8871a24301f42e74e59MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/22955/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/22955oai:bibliotecadigital.udea.edu.co:10495/229552022-10-28 20:42:32.929Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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