Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913)
ABSTRACT: Spermatozoa in Characid fish remain immobile in seminal plasma and are activated when freed into water where the ionic balance seems to be the main factor starting the activation process. This process was the target of the present study with emphasis on the activation of motility and on mo...
- Autores:
-
Tabares Serna, Carlos Javier
Ruiz Cortés, Zulma Tatiana
Arboleda, Lucy
Olivera Ángel, Martha
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2007
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/22955
- Acceso en línea:
- http://hdl.handle.net/10495/22955
https://revistas.unal.edu.co/index.php/actabiol/article/view/27173
- Palabra clave:
- Membrane Potentials
Potenciales de la Membrana
Antagonistas del calcio
Calcium - Antagonists
Ionic balance
Equilibrio ionico
Oxonol
http://aims.fao.org/aos/agrovoc/c_3933
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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|
dc.title.spa.fl_str_mv |
Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913) |
dc.title.alternative.spa.fl_str_mv |
Efecto de algunos iones sobre la activación espermática en Brycon henni (Eigenmann 1913) |
title |
Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913) |
spellingShingle |
Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913) Membrane Potentials Potenciales de la Membrana Antagonistas del calcio Calcium - Antagonists Ionic balance Equilibrio ionico Oxonol http://aims.fao.org/aos/agrovoc/c_3933 |
title_short |
Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913) |
title_full |
Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913) |
title_fullStr |
Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913) |
title_full_unstemmed |
Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913) |
title_sort |
Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913) |
dc.creator.fl_str_mv |
Tabares Serna, Carlos Javier Ruiz Cortés, Zulma Tatiana Arboleda, Lucy Olivera Ángel, Martha |
dc.contributor.author.none.fl_str_mv |
Tabares Serna, Carlos Javier Ruiz Cortés, Zulma Tatiana Arboleda, Lucy Olivera Ángel, Martha |
dc.subject.decs.none.fl_str_mv |
Membrane Potentials Potenciales de la Membrana |
topic |
Membrane Potentials Potenciales de la Membrana Antagonistas del calcio Calcium - Antagonists Ionic balance Equilibrio ionico Oxonol http://aims.fao.org/aos/agrovoc/c_3933 |
dc.subject.lemb.none.fl_str_mv |
Antagonistas del calcio Calcium - Antagonists |
dc.subject.agrovoc.none.fl_str_mv |
Ionic balance Equilibrio ionico |
dc.subject.proposal.spa.fl_str_mv |
Oxonol |
dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_3933 |
description |
ABSTRACT: Spermatozoa in Characid fish remain immobile in seminal plasma and are activated when freed into water where the ionic balance seems to be the main factor starting the activation process. This process was the target of the present study with emphasis on the activation of motility and on motility maintenance over time. The effect of isosmotic solutions was analyzed taking into account the possible combinations of the following ions, Ca2, K', Mg2 and Na' as well as the effect of channel blocking agents. The parameters measured were cells with motility (%), duration of motility (s), plasma membrane potential, and the effect of channel blockers on activation time and on motility. There was an increased motility when the semen was incubated in solutions containing K' (p<0.05) compared with the control (CaNaMgK solution); the longest duration of motility was attained when the incubation was performed in solutions containing Na and Mg2 (p<O.OS). All solutions induced a change in membrane potential detected after 15 s of activation. Blocking K', Ca2, and Na channels did not alter motility but decreased the activation time (p<0.05). Potassium induced activation at all concentrations up to 105 mM, but motility was drastically decreased at concentrations higher than 140 mM (p<0.05). The conclusion is that interaction of the ionic environment with the cell membrane leads to changes in membrane potential and intracellular signalling that trigger sperm motility in Brycon henni |
publishDate |
2007 |
dc.date.issued.none.fl_str_mv |
2007 |
dc.date.accessioned.none.fl_str_mv |
2021-10-06T21:13:50Z |
dc.date.available.none.fl_str_mv |
2021-10-06T21:13:50Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.citation.spa.fl_str_mv |
Tabares, J., Ruíz, T., Arboleda, L., & Olivera, M. (2007). Efecto de algunos iones sobre la activación espermática en Brycon henni (Eigenmann 1913). Acta Biológica Colombiana, 12(1), 87-97. Recuperado a partir de https://revistas.unal.edu.co/index.php/actabiol/article/view/27173 |
dc.identifier.issn.none.fl_str_mv |
0120-548X |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/22955 |
dc.identifier.eissn.none.fl_str_mv |
1900-1649 |
dc.identifier.url.spa.fl_str_mv |
https://revistas.unal.edu.co/index.php/actabiol/article/view/27173 |
identifier_str_mv |
Tabares, J., Ruíz, T., Arboleda, L., & Olivera, M. (2007). Efecto de algunos iones sobre la activación espermática en Brycon henni (Eigenmann 1913). Acta Biológica Colombiana, 12(1), 87-97. Recuperado a partir de https://revistas.unal.edu.co/index.php/actabiol/article/view/27173 0120-548X 1900-1649 |
url |
http://hdl.handle.net/10495/22955 https://revistas.unal.edu.co/index.php/actabiol/article/view/27173 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Acta Biolo. Colomb. |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-sa/4.0/ |
dc.format.extent.spa.fl_str_mv |
11 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Biología |
dc.publisher.group.spa.fl_str_mv |
Biogénesis |
dc.publisher.place.spa.fl_str_mv |
Bogotá, Colombia |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
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MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad de Antioquia |
repository.mail.fl_str_mv |
andres.perez@udea.edu.co |
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1812173213099098112 |
spelling |
Tabares Serna, Carlos JavierRuiz Cortés, Zulma TatianaArboleda, LucyOlivera Ángel, Martha2021-10-06T21:13:50Z2021-10-06T21:13:50Z2007Tabares, J., Ruíz, T., Arboleda, L., & Olivera, M. (2007). Efecto de algunos iones sobre la activación espermática en Brycon henni (Eigenmann 1913). Acta Biológica Colombiana, 12(1), 87-97. Recuperado a partir de https://revistas.unal.edu.co/index.php/actabiol/article/view/271730120-548Xhttp://hdl.handle.net/10495/229551900-1649https://revistas.unal.edu.co/index.php/actabiol/article/view/27173ABSTRACT: Spermatozoa in Characid fish remain immobile in seminal plasma and are activated when freed into water where the ionic balance seems to be the main factor starting the activation process. This process was the target of the present study with emphasis on the activation of motility and on motility maintenance over time. The effect of isosmotic solutions was analyzed taking into account the possible combinations of the following ions, Ca2, K', Mg2 and Na' as well as the effect of channel blocking agents. The parameters measured were cells with motility (%), duration of motility (s), plasma membrane potential, and the effect of channel blockers on activation time and on motility. There was an increased motility when the semen was incubated in solutions containing K' (p<0.05) compared with the control (CaNaMgK solution); the longest duration of motility was attained when the incubation was performed in solutions containing Na and Mg2 (p<O.OS). All solutions induced a change in membrane potential detected after 15 s of activation. Blocking K', Ca2, and Na channels did not alter motility but decreased the activation time (p<0.05). Potassium induced activation at all concentrations up to 105 mM, but motility was drastically decreased at concentrations higher than 140 mM (p<0.05). The conclusion is that interaction of the ionic environment with the cell membrane leads to changes in membrane potential and intracellular signalling that trigger sperm motility in Brycon henniRESUMEN: Los espermatozoides en los carácidos se mantienen inm6viles en plasma seminal, activándose cuando se liberan en el agua. EI objetivo de este estudio fue conocer algunos de los mecanismos que inducen la activación espermática (porcentaje de espermatozoides que adquieren movilidad y el tiempo que dura la misma). EI efecto de las soluciones isoosmóticas, se analiz6 considerando las combinaciones de los iones Ca", K', Mg2 Y Na, y el efecto de algunos bloqueadores de canales i6nicos. Se evalu6 el porcentaje y duración de la movilidad, as! como el potencial de la membrana plasmática, La motilidad espermática fue mayor cuando el semen se incubo en las soluciones que contengan K' (p<O,OS) comparado con el control positivo. La mayor duración de la movilidad se obtuvo cuando se incubaron los espermatozoides en soluciones que contengan Na' y Mg2 (p<O,OS). Todas las soluciones probadas indujeron un cambio en el potencial de membrana, detectado después de 15 segundos de Ia activaci6n. Bloqueo de los canales de K', Ca' y Na' no altere la movilidad, sino que disminuyo el tiempo de activaci6n (p<0,05). Los iones potasio indujeron la activaci6n en codas las concentraciones hasta 105 mM, pero la movilidad disminuy6 drásticamente cuando las concentraciones fueron mayores de 140 mM (p<0,05). Se concluye que la interacción del ambiente iónico con la membrana de la célula conduce a cambios en el potencial de la membrana que conllevan a señales intracelulares que desencadenan la movilidad del esperma.COL006656111application/pdfengUniversidad Nacional de Colombia, Facultad de Ciencias, Departamento de BiologíaBiogénesisBogotá, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Effect of some ions on sperm activation in Brycon henni (EIGENMANN 1913)Efecto de algunos iones sobre la activación espermática en Brycon henni (Eigenmann 1913)Membrane PotentialsPotenciales de la MembranaAntagonistas del calcioCalcium - AntagonistsIonic balanceEquilibrio ionicoOxonolhttp://aims.fao.org/aos/agrovoc/c_3933Acta Biolo. Colomb.Acta Biológica Colombiana8798121CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051https://bibliotecadigital.udea.edu.co/bitstream/10495/22955/2/license_rdfe2060682c9c70d4d30c83c51448f4eedMD52ORIGINALRuizTatiana_2007_EfectosIonesActivacionEspermatica.pdfRuizTatiana_2007_EfectosIonesActivacionEspermatica.pdfArtículo de investigaciónapplication/pdf3230201https://bibliotecadigital.udea.edu.co/bitstream/10495/22955/1/RuizTatiana_2007_EfectosIonesActivacionEspermatica.pdfd25744236c2bb8871a24301f42e74e59MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/22955/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/22955oai:bibliotecadigital.udea.edu.co:10495/229552022-10-28 20:42:32.929Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |