Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch
ABSTRACT : Bacillus cereus is a human pathogenic bacterium found in foods with the potential to cause emesis and diarrhea. This study estimated the presence, toxigenic and genomic diversity of B. cereus s.l. obtained from cassava starch samples collected in bakeries and powdered food companies in Me...
- Autores:
-
Sánchez Chica, Jennifer
Correa Ochoa, Margarita María
Aceves Diez, Ángel
Castañeda Sandoval, Laura Margatira
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2021
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/29476
- Acceso en línea:
- http://hdl.handle.net/10495/29476
- Palabra clave:
- Almidones y Féculas
Starch and Fecula
Bacillus cereus
Enterotoxinas
Enterotoxins
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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| dc.title.spa.fl_str_mv |
Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch |
| title |
Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch |
| spellingShingle |
Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch Almidones y Féculas Starch and Fecula Bacillus cereus Enterotoxinas Enterotoxins |
| title_short |
Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch |
| title_full |
Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch |
| title_fullStr |
Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch |
| title_full_unstemmed |
Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch |
| title_sort |
Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava Starch |
| dc.creator.fl_str_mv |
Sánchez Chica, Jennifer Correa Ochoa, Margarita María Aceves Diez, Ángel Castañeda Sandoval, Laura Margatira |
| dc.contributor.author.none.fl_str_mv |
Sánchez Chica, Jennifer Correa Ochoa, Margarita María Aceves Diez, Ángel Castañeda Sandoval, Laura Margatira |
| dc.subject.decs.none.fl_str_mv |
Almidones y Féculas Starch and Fecula Bacillus cereus Enterotoxinas Enterotoxins |
| topic |
Almidones y Féculas Starch and Fecula Bacillus cereus Enterotoxinas Enterotoxins |
| description |
ABSTRACT : Bacillus cereus is a human pathogenic bacterium found in foods with the potential to cause emesis and diarrhea. This study estimated the presence, toxigenic and genomic diversity of B. cereus s.l. obtained from cassava starch samples collected in bakeries and powdered food companies in Medellín (Colombia). Bacillus cereus s.l. was found in 43 of 75 (57%) cassava starch samples and 98 isolates were obtained. The nheABC, hblCDAB, cytK2, entFM and cesB toxin genes were detected by multiplex PCR and the most frequent operon was nheABC, whereas cesB gene was not found. Twelve toxigenic profiles were determined by the detection of toxin genes, and the most frequent profiles harbored all enterotoxin genes. A broad genomic diversity was detected according to GTG5 -PCR fingerprinting results with 76 B. cereus s.l. grouped in sixteen clusters and the 22 isolates clustering separately. No relationship was observed between genomic background and toxigenic profiles. In general, the results showed a high genomic and enterotoxigenic diversity in B. cereus s.l. found in cassava starch. These results should incentive future studies to understand the distribution of B. cereus s.l. isolated on raw materials in comparison with finished products. |
| publishDate |
2021 |
| dc.date.issued.none.fl_str_mv |
2021 |
| dc.date.accessioned.none.fl_str_mv |
2022-06-29T19:59:12Z |
| dc.date.available.none.fl_str_mv |
2022-06-29T19:59:12Z |
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info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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Sánchez-Chica J, Correa MM, Aceves-Diez AE, Castañeda-Sandoval LM. Enterotoxin Gene Distribution and Genotypes of Bacillus cereussensu lato Isolated from Cassava Starch. Toxins (Basel). 2021 Feb 10;13(2):131. doi: 10.3390/toxins13020131. |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/29476 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/toxins13020131. |
| dc.identifier.eissn.none.fl_str_mv |
2072-6651 |
| identifier_str_mv |
Sánchez-Chica J, Correa MM, Aceves-Diez AE, Castañeda-Sandoval LM. Enterotoxin Gene Distribution and Genotypes of Bacillus cereussensu lato Isolated from Cassava Starch. Toxins (Basel). 2021 Feb 10;13(2):131. doi: 10.3390/toxins13020131. 10.3390/toxins13020131. 2072-6651 |
| url |
http://hdl.handle.net/10495/29476 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Toxins |
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info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/2.5/co/ |
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https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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http://creativecommons.org/licenses/by/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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10 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
MDPI (Multidisciplinary Digital Publishing Institute) |
| dc.publisher.group.spa.fl_str_mv |
Microbiología Molecular |
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Basilea, Suiza |
| institution |
Universidad de Antioquia |
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https://bibliotecadigital.udea.edu.co/bitstream/10495/29476/1/SanchezJennifer_2021_Enterotoxin_Gene_Distribution.pdf https://bibliotecadigital.udea.edu.co/bitstream/10495/29476/2/license_rdf https://bibliotecadigital.udea.edu.co/bitstream/10495/29476/3/license.txt |
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andres.perez@udea.edu.co |
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Sánchez Chica, JenniferCorrea Ochoa, Margarita MaríaAceves Diez, ÁngelCastañeda Sandoval, Laura Margatira2022-06-29T19:59:12Z2022-06-29T19:59:12Z2021Sánchez-Chica J, Correa MM, Aceves-Diez AE, Castañeda-Sandoval LM. Enterotoxin Gene Distribution and Genotypes of Bacillus cereussensu lato Isolated from Cassava Starch. Toxins (Basel). 2021 Feb 10;13(2):131. doi: 10.3390/toxins13020131.http://hdl.handle.net/10495/2947610.3390/toxins13020131.2072-6651ABSTRACT : Bacillus cereus is a human pathogenic bacterium found in foods with the potential to cause emesis and diarrhea. This study estimated the presence, toxigenic and genomic diversity of B. cereus s.l. obtained from cassava starch samples collected in bakeries and powdered food companies in Medellín (Colombia). Bacillus cereus s.l. was found in 43 of 75 (57%) cassava starch samples and 98 isolates were obtained. The nheABC, hblCDAB, cytK2, entFM and cesB toxin genes were detected by multiplex PCR and the most frequent operon was nheABC, whereas cesB gene was not found. Twelve toxigenic profiles were determined by the detection of toxin genes, and the most frequent profiles harbored all enterotoxin genes. A broad genomic diversity was detected according to GTG5 -PCR fingerprinting results with 76 B. cereus s.l. grouped in sixteen clusters and the 22 isolates clustering separately. No relationship was observed between genomic background and toxigenic profiles. In general, the results showed a high genomic and enterotoxigenic diversity in B. cereus s.l. found in cassava starch. These results should incentive future studies to understand the distribution of B. cereus s.l. isolated on raw materials in comparison with finished products.COL001374610application/pdfengMDPI (Multidisciplinary Digital Publishing Institute)Microbiología MolecularBasilea, Suizainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Enterotoxin Gene Distribution and Genotypes of Bacillus cereus sensu lato Isolated from Cassava StarchAlmidones y FéculasStarch and FeculaBacillus cereusEnterotoxinasEnterotoxinsToxinsToxins110132ORIGINALSanchezJennifer_2021_Enterotoxin_Gene_Distribution.pdfSanchezJennifer_2021_Enterotoxin_Gene_Distribution.pdfArtículo de investigaciónapplication/pdf1751092https://bibliotecadigital.udea.edu.co/bitstream/10495/29476/1/SanchezJennifer_2021_Enterotoxin_Gene_Distribution.pdf64efc680fca6461166b5056c13319b23MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstream/10495/29476/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/29476/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/29476oai:bibliotecadigital.udea.edu.co:10495/294762022-06-29 14:59:12.561Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.coTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo= |