Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus
ABSTRACT: Objective In various chronic inflammatory processes, both the proportion and numbers of monocyte subsets are altered. In systemic lupus erythematosus (SLE), this has not been clearly determined. The monocyte subpopulations in patients with SLE, patients with other autoimmune diseases, and...
- Autores:
-
Burbano Arciniegas, Catalina
Vásquez Duque, Gloria María
Rojas López, Mauricio
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2014
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/25903
- Acceso en línea:
- http://hdl.handle.net/10495/25903
- Palabra clave:
- Monocitos
Monocytes
Receptores de IgG
Receptors, IgG
Receptores de Lipopolisacáridos
Lipopolysaccharide Receptors
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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|
dc.title.spa.fl_str_mv |
Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus |
title |
Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus |
spellingShingle |
Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus Monocitos Monocytes Receptores de IgG Receptors, IgG Receptores de Lipopolisacáridos Lipopolysaccharide Receptors |
title_short |
Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus |
title_full |
Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus |
title_fullStr |
Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus |
title_full_unstemmed |
Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus |
title_sort |
Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus |
dc.creator.fl_str_mv |
Burbano Arciniegas, Catalina Vásquez Duque, Gloria María Rojas López, Mauricio |
dc.contributor.author.none.fl_str_mv |
Burbano Arciniegas, Catalina Vásquez Duque, Gloria María Rojas López, Mauricio |
dc.subject.decs.none.fl_str_mv |
Monocitos Monocytes Receptores de IgG Receptors, IgG Receptores de Lipopolisacáridos Lipopolysaccharide Receptors |
topic |
Monocitos Monocytes Receptores de IgG Receptors, IgG Receptores de Lipopolisacáridos Lipopolysaccharide Receptors |
description |
ABSTRACT: Objective In various chronic inflammatory processes, both the proportion and numbers of monocyte subsets are altered. In systemic lupus erythematosus (SLE), this has not been clearly determined. The monocyte subpopulations in patients with SLE, patients with other autoimmune diseases, and healthy controls were evaluated. The effects of nonclassic monocytes and apoptotic cells (ACs) on the differentiation and function of CD14++CD16− monocytes were also studied. Methods Monocyte subpopulations derived from the blood samples of SLE patients (n = 88), patients with other autoimmune diseases (n = 37), and healthy control subjects (n = 61) were separated by fluorescence-activated cell sorting. To evaluate the effect of CD14+CD16++ monocytes and ACs on the differentiation of CD14++CD16− monocytes, we developed a coculture model of highly purified sorted monocyte subpopulations, which were reconstituted with defined proportions of CD14++CD16− and CD14+CD16++ monocytes in the presence or absence of ACs. After differentiation into macrophages, CD3+ lymphocytes were added, and the proliferating cells and CD3+IFNγ+ cells were evaluated. A cytokine bead array panel was used to test the coculture supernatants. Results There was a reduction in CD14+CD16++ monocytes in patients with active SLE. Monocytes from SLE patients had decreased expression of HLA–DR and decreased ability to bind and phagocytize ACs. In healthy controls, but not SLE patients, treatment with macrophages derived from CD14+CD16++ monocytes reduced T cell proliferation and proliferating CD3+IFNγ+ cells and increased the accumulation of tumor necrosis factor α, interleukin-10 (IL-10), and IL-1β. Conclusion Our findings show that CD14+CD16++ monocytes, a population that is reduced and nonfunctional in SLE patients, have modulatory effects on CD14++CD16− monocytes and T cells. |
publishDate |
2014 |
dc.date.issued.none.fl_str_mv |
2014 |
dc.date.accessioned.none.fl_str_mv |
2022-02-09T14:05:38Z |
dc.date.available.none.fl_str_mv |
2022-02-09T14:05:38Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.issn.none.fl_str_mv |
0004-3591 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/25903 |
dc.identifier.doi.none.fl_str_mv |
10.1002/art.38860 |
dc.identifier.eissn.none.fl_str_mv |
1529-0131 |
identifier_str_mv |
0004-3591 10.1002/art.38860 1529-0131 |
url |
http://hdl.handle.net/10495/25903 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Arthritis Rheum. |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.format.extent.spa.fl_str_mv |
11 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
American College of Rheumatology Wiley |
dc.publisher.group.spa.fl_str_mv |
Grupo de Inmunología Celular e Inmunogenética |
dc.publisher.place.spa.fl_str_mv |
Malden, Estados Unidos |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstream/10495/25903/2/license_rdf https://bibliotecadigital.udea.edu.co/bitstream/10495/25903/3/license.txt https://bibliotecadigital.udea.edu.co/bitstream/10495/25903/1/VasquezGloria_2014_MonocytesCD14CD16.pdf |
bitstream.checksum.fl_str_mv |
b88b088d9957e670ce3b3fbe2eedbc13 8a4605be74aa9ea9d79846c1fba20a33 c2e86f1b3fb44ed35e4ce5be7e2e92d9 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad de Antioquia |
repository.mail.fl_str_mv |
andres.perez@udea.edu.co |
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1812173179723972608 |
spelling |
Burbano Arciniegas, CatalinaVásquez Duque, Gloria MaríaRojas López, Mauricio2022-02-09T14:05:38Z2022-02-09T14:05:38Z20140004-3591http://hdl.handle.net/10495/2590310.1002/art.388601529-0131ABSTRACT: Objective In various chronic inflammatory processes, both the proportion and numbers of monocyte subsets are altered. In systemic lupus erythematosus (SLE), this has not been clearly determined. The monocyte subpopulations in patients with SLE, patients with other autoimmune diseases, and healthy controls were evaluated. The effects of nonclassic monocytes and apoptotic cells (ACs) on the differentiation and function of CD14++CD16− monocytes were also studied. Methods Monocyte subpopulations derived from the blood samples of SLE patients (n = 88), patients with other autoimmune diseases (n = 37), and healthy control subjects (n = 61) were separated by fluorescence-activated cell sorting. To evaluate the effect of CD14+CD16++ monocytes and ACs on the differentiation of CD14++CD16− monocytes, we developed a coculture model of highly purified sorted monocyte subpopulations, which were reconstituted with defined proportions of CD14++CD16− and CD14+CD16++ monocytes in the presence or absence of ACs. After differentiation into macrophages, CD3+ lymphocytes were added, and the proliferating cells and CD3+IFNγ+ cells were evaluated. A cytokine bead array panel was used to test the coculture supernatants. Results There was a reduction in CD14+CD16++ monocytes in patients with active SLE. Monocytes from SLE patients had decreased expression of HLA–DR and decreased ability to bind and phagocytize ACs. In healthy controls, but not SLE patients, treatment with macrophages derived from CD14+CD16++ monocytes reduced T cell proliferation and proliferating CD3+IFNγ+ cells and increased the accumulation of tumor necrosis factor α, interleukin-10 (IL-10), and IL-1β. Conclusion Our findings show that CD14+CD16++ monocytes, a population that is reduced and nonfunctional in SLE patients, have modulatory effects on CD14++CD16− monocytes and T cells.COL0000962COL000863911application/pdfengAmerican College of RheumatologyWileyGrupo de Inmunología Celular e InmunogenéticaMalden, Estados Unidosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus ErythematosusMonocitosMonocytesReceptores de IgGReceptors, IgGReceptores de LipopolisacáridosLipopolysaccharide ReceptorsArthritis Rheum.Arthritis & Rheumatism337133816612CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823https://bibliotecadigital.udea.edu.co/bitstream/10495/25903/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/25903/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINALVasquezGloria_2014_MonocytesCD14CD16.pdfVasquezGloria_2014_MonocytesCD14CD16.pdfArtículo de investigaciónapplication/pdf696231https://bibliotecadigital.udea.edu.co/bitstream/10495/25903/1/VasquezGloria_2014_MonocytesCD14CD16.pdfc2e86f1b3fb44ed35e4ce5be7e2e92d9MD5110495/25903oai:bibliotecadigital.udea.edu.co:10495/259032022-11-18 11:03:51.207Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |