Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus

ABSTRACT: Objective In various chronic inflammatory processes, both the proportion and numbers of monocyte subsets are altered. In systemic lupus erythematosus (SLE), this has not been clearly determined. The monocyte subpopulations in patients with SLE, patients with other autoimmune diseases, and...

Full description

Autores:
Burbano Arciniegas, Catalina
Vásquez Duque, Gloria María
Rojas López, Mauricio
Tipo de recurso:
Article of investigation
Fecha de publicación:
2014
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/25903
Acceso en línea:
http://hdl.handle.net/10495/25903
Palabra clave:
Monocitos
Monocytes
Receptores de IgG
Receptors, IgG
Receptores de Lipopolisacáridos
Lipopolysaccharide Receptors
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-nd/2.5/co/
id UDEA2_39b5ffa7ffbee0afa5220428882c1b55
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/25903
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus
title Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus
spellingShingle Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus
Monocitos
Monocytes
Receptores de IgG
Receptors, IgG
Receptores de Lipopolisacáridos
Lipopolysaccharide Receptors
title_short Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus
title_full Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus
title_fullStr Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus
title_full_unstemmed Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus
title_sort Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus Erythematosus
dc.creator.fl_str_mv Burbano Arciniegas, Catalina
Vásquez Duque, Gloria María
Rojas López, Mauricio
dc.contributor.author.none.fl_str_mv Burbano Arciniegas, Catalina
Vásquez Duque, Gloria María
Rojas López, Mauricio
dc.subject.decs.none.fl_str_mv Monocitos
Monocytes
Receptores de IgG
Receptors, IgG
Receptores de Lipopolisacáridos
Lipopolysaccharide Receptors
topic Monocitos
Monocytes
Receptores de IgG
Receptors, IgG
Receptores de Lipopolisacáridos
Lipopolysaccharide Receptors
description ABSTRACT: Objective In various chronic inflammatory processes, both the proportion and numbers of monocyte subsets are altered. In systemic lupus erythematosus (SLE), this has not been clearly determined. The monocyte subpopulations in patients with SLE, patients with other autoimmune diseases, and healthy controls were evaluated. The effects of nonclassic monocytes and apoptotic cells (ACs) on the differentiation and function of CD14++CD16− monocytes were also studied. Methods Monocyte subpopulations derived from the blood samples of SLE patients (n = 88), patients with other autoimmune diseases (n = 37), and healthy control subjects (n = 61) were separated by fluorescence-activated cell sorting. To evaluate the effect of CD14+CD16++ monocytes and ACs on the differentiation of CD14++CD16− monocytes, we developed a coculture model of highly purified sorted monocyte subpopulations, which were reconstituted with defined proportions of CD14++CD16− and CD14+CD16++ monocytes in the presence or absence of ACs. After differentiation into macrophages, CD3+ lymphocytes were added, and the proliferating cells and CD3+IFNγ+ cells were evaluated. A cytokine bead array panel was used to test the coculture supernatants. Results There was a reduction in CD14+CD16++ monocytes in patients with active SLE. Monocytes from SLE patients had decreased expression of HLA–DR and decreased ability to bind and phagocytize ACs. In healthy controls, but not SLE patients, treatment with macrophages derived from CD14+CD16++ monocytes reduced T cell proliferation and proliferating CD3+IFNγ+ cells and increased the accumulation of tumor necrosis factor α, interleukin-10 (IL-10), and IL-1β. Conclusion Our findings show that CD14+CD16++ monocytes, a population that is reduced and nonfunctional in SLE patients, have modulatory effects on CD14++CD16− monocytes and T cells.
publishDate 2014
dc.date.issued.none.fl_str_mv 2014
dc.date.accessioned.none.fl_str_mv 2022-02-09T14:05:38Z
dc.date.available.none.fl_str_mv 2022-02-09T14:05:38Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.local.spa.fl_str_mv Artículo de investigación
format http://purl.org/coar/resource_type/c_2df8fbb1
status_str publishedVersion
dc.identifier.issn.none.fl_str_mv 0004-3591
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/25903
dc.identifier.doi.none.fl_str_mv 10.1002/art.38860
dc.identifier.eissn.none.fl_str_mv 1529-0131
identifier_str_mv 0004-3591
10.1002/art.38860
1529-0131
url http://hdl.handle.net/10495/25903
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Arthritis Rheum.
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/co/
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.extent.spa.fl_str_mv 11
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv American College of Rheumatology
Wiley
dc.publisher.group.spa.fl_str_mv Grupo de Inmunología Celular e Inmunogenética
dc.publisher.place.spa.fl_str_mv Malden, Estados Unidos
institution Universidad de Antioquia
bitstream.url.fl_str_mv https://bibliotecadigital.udea.edu.co/bitstream/10495/25903/2/license_rdf
https://bibliotecadigital.udea.edu.co/bitstream/10495/25903/3/license.txt
https://bibliotecadigital.udea.edu.co/bitstream/10495/25903/1/VasquezGloria_2014_MonocytesCD14CD16.pdf
bitstream.checksum.fl_str_mv b88b088d9957e670ce3b3fbe2eedbc13
8a4605be74aa9ea9d79846c1fba20a33
c2e86f1b3fb44ed35e4ce5be7e2e92d9
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
repository.mail.fl_str_mv andres.perez@udea.edu.co
_version_ 1812173179723972608
spelling Burbano Arciniegas, CatalinaVásquez Duque, Gloria MaríaRojas López, Mauricio2022-02-09T14:05:38Z2022-02-09T14:05:38Z20140004-3591http://hdl.handle.net/10495/2590310.1002/art.388601529-0131ABSTRACT: Objective In various chronic inflammatory processes, both the proportion and numbers of monocyte subsets are altered. In systemic lupus erythematosus (SLE), this has not been clearly determined. The monocyte subpopulations in patients with SLE, patients with other autoimmune diseases, and healthy controls were evaluated. The effects of nonclassic monocytes and apoptotic cells (ACs) on the differentiation and function of CD14++CD16− monocytes were also studied. Methods Monocyte subpopulations derived from the blood samples of SLE patients (n = 88), patients with other autoimmune diseases (n = 37), and healthy control subjects (n = 61) were separated by fluorescence-activated cell sorting. To evaluate the effect of CD14+CD16++ monocytes and ACs on the differentiation of CD14++CD16− monocytes, we developed a coculture model of highly purified sorted monocyte subpopulations, which were reconstituted with defined proportions of CD14++CD16− and CD14+CD16++ monocytes in the presence or absence of ACs. After differentiation into macrophages, CD3+ lymphocytes were added, and the proliferating cells and CD3+IFNγ+ cells were evaluated. A cytokine bead array panel was used to test the coculture supernatants. Results There was a reduction in CD14+CD16++ monocytes in patients with active SLE. Monocytes from SLE patients had decreased expression of HLA–DR and decreased ability to bind and phagocytize ACs. In healthy controls, but not SLE patients, treatment with macrophages derived from CD14+CD16++ monocytes reduced T cell proliferation and proliferating CD3+IFNγ+ cells and increased the accumulation of tumor necrosis factor α, interleukin-10 (IL-10), and IL-1β. Conclusion Our findings show that CD14+CD16++ monocytes, a population that is reduced and nonfunctional in SLE patients, have modulatory effects on CD14++CD16− monocytes and T cells.COL0000962COL000863911application/pdfengAmerican College of RheumatologyWileyGrupo de Inmunología Celular e InmunogenéticaMalden, Estados Unidosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Modulatory Effects of CD14+CD16++ Monocytes on CD14++CD16− Monocytes : A Possible Explanation of Monocyte Alterations in Systemic Lupus ErythematosusMonocitosMonocytesReceptores de IgGReceptors, IgGReceptores de LipopolisacáridosLipopolysaccharide ReceptorsArthritis Rheum.Arthritis & Rheumatism337133816612CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8823https://bibliotecadigital.udea.edu.co/bitstream/10495/25903/2/license_rdfb88b088d9957e670ce3b3fbe2eedbc13MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/25903/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINALVasquezGloria_2014_MonocytesCD14CD16.pdfVasquezGloria_2014_MonocytesCD14CD16.pdfArtículo de investigaciónapplication/pdf696231https://bibliotecadigital.udea.edu.co/bitstream/10495/25903/1/VasquezGloria_2014_MonocytesCD14CD16.pdfc2e86f1b3fb44ed35e4ce5be7e2e92d9MD5110495/25903oai:bibliotecadigital.udea.edu.co:10495/259032022-11-18 11:03:51.207Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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