Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in Colombia

ABSTRACT : Objectives: Carbapenem-resistant Gram-negative bacilli (CRGNB) have been reported in different wastewater treatment plants (WWTPs) throughout the world; however, few studies have described the antimicrobial resistance profile in different CRGNB throughout WWTPs, information that would ide...

Full description

Autores:
Rodríguez Tamayo, Erika Andrea
Garzón Hernández, Lina Marcela
Gómez Alzate, Iván Darío
Jiménez Quiceno, Judy Natalia
Tipo de recurso:
Article of investigation
Fecha de publicación:
2020
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/31153
Acceso en línea:
https://hdl.handle.net/10495/31153
Palabra clave:
Plantas de Tratamiento de Aguas Residuales
Wastewater Treatment Plants
Resistencia a Múltiples Medicamentos
Drug Resistance, Multiple
Carbapenémicos
Carbapenems
Enterobacteriaceae
Aeromonadaceae
ARN Ribosómico 16S
RNA, Ribosomal, 16S
Purificación del Agua
Water Purification
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
id UDEA2_2dc2a6519dc9be786770c39ef01abb8b
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/31153
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in Colombia
title Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in Colombia
spellingShingle Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in Colombia
Plantas de Tratamiento de Aguas Residuales
Wastewater Treatment Plants
Resistencia a Múltiples Medicamentos
Drug Resistance, Multiple
Carbapenémicos
Carbapenems
Enterobacteriaceae
Aeromonadaceae
ARN Ribosómico 16S
RNA, Ribosomal, 16S
Purificación del Agua
Water Purification
title_short Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in Colombia
title_full Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in Colombia
title_fullStr Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in Colombia
title_full_unstemmed Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in Colombia
title_sort Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in Colombia
dc.creator.fl_str_mv Rodríguez Tamayo, Erika Andrea
Garzón Hernández, Lina Marcela
Gómez Alzate, Iván Darío
Jiménez Quiceno, Judy Natalia
dc.contributor.author.none.fl_str_mv Rodríguez Tamayo, Erika Andrea
Garzón Hernández, Lina Marcela
Gómez Alzate, Iván Darío
Jiménez Quiceno, Judy Natalia
dc.subject.decs.none.fl_str_mv Plantas de Tratamiento de Aguas Residuales
Wastewater Treatment Plants
Resistencia a Múltiples Medicamentos
Drug Resistance, Multiple
Carbapenémicos
Carbapenems
Enterobacteriaceae
Aeromonadaceae
ARN Ribosómico 16S
RNA, Ribosomal, 16S
Purificación del Agua
Water Purification
topic Plantas de Tratamiento de Aguas Residuales
Wastewater Treatment Plants
Resistencia a Múltiples Medicamentos
Drug Resistance, Multiple
Carbapenémicos
Carbapenems
Enterobacteriaceae
Aeromonadaceae
ARN Ribosómico 16S
RNA, Ribosomal, 16S
Purificación del Agua
Water Purification
description ABSTRACT : Objectives: Carbapenem-resistant Gram-negative bacilli (CRGNB) have been reported in different wastewater treatment plants (WWTPs) throughout the world; however, few studies have described the antimicrobial resistance profile in different CRGNB throughout WWTPs, information that would identify points of selection of resistant bacteria. The objective of this work was to characterize the resistance profile of CRGNB harbouring blaKPC-2 from a Colombian WWTP. Methods: Six samples were taken from four points of a WWTP. CRGNB were selected in chromID1 CARBA and identified by 16S rRNA. Carbapenemases were determined by polymerase chain reaction (PCR), and susceptibility was assessed using VITEK2. Results: One hundred and forty-two CRGNB harbouring blaKPC-2 were detected: 41% corresponded to Aeromonas spp. (n = 58) and 59% to Enterobacteriaceae. To establish the resistance profile, 50% of the isolates were selected proportionally by family and sampling point (26 Aeromonadaceae and 45 Enterobacteriaceae). All Enterobacteriaceae showed resistance to carbapenems and penicillins + inhibitors, high percentages of resistance to ceftriaxone (88.9%), and ciprofloxacin (44.4%), and low resistance to other antibiotics (>30%). In Aeromonadaceae, 76.9% were resistant to ceftriaxone, 58% to carbapenems, and 65.4% to ciprofloxacin. Twenty-one resistance profiles were observed, the most common of which were resistant to penicillins + inhibitor, cephalosporins (third to fourth generation), and carbapenems (19%). The percentage of multidrug resistance was 91% and was similar at all points of the WWTP. Conclusions: The high frequency of multidrug resistance and great diversity of resistance profiles observed throughout the WWTP is of concern, and shows the role of WWTP as a reservoir and dissemination source of antimicrobial resistance to water sources.
publishDate 2020
dc.date.issued.none.fl_str_mv 2020
dc.date.accessioned.none.fl_str_mv 2022-10-08T21:03:06Z
dc.date.available.none.fl_str_mv 2022-10-08T21:03:06Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.spa.fl_str_mv Rodríguez EA, Garzón LM, Gómez ID, Jiménez JN. Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in Colombia. J Glob Antimicrob Resist. 2020 Sep;22:358-366. doi: 10.1016/j.jgar.2020.02.033.
dc.identifier.issn.none.fl_str_mv 2213-7165
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10495/31153
dc.identifier.doi.none.fl_str_mv 10.1016/j.jgar.2020.02.033
dc.identifier.eissn.none.fl_str_mv 2213-7173
identifier_str_mv Rodríguez EA, Garzón LM, Gómez ID, Jiménez JN. Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in Colombia. J Glob Antimicrob Resist. 2020 Sep;22:358-366. doi: 10.1016/j.jgar.2020.02.033.
2213-7165
10.1016/j.jgar.2020.02.033
2213-7173
url https://hdl.handle.net/10495/31153
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv J. Glob. Antimicrob. Resist.
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dc.publisher.spa.fl_str_mv Elsevier
dc.publisher.group.spa.fl_str_mv Grupo de Investigación en Microbiología Básica y Aplicada-Microba
dc.publisher.place.spa.fl_str_mv Ámsterdam, Países Bajos
institution Universidad de Antioquia
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spelling Rodríguez Tamayo, Erika AndreaGarzón Hernández, Lina MarcelaGómez Alzate, Iván DaríoJiménez Quiceno, Judy Natalia2022-10-08T21:03:06Z2022-10-08T21:03:06Z2020Rodríguez EA, Garzón LM, Gómez ID, Jiménez JN. Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in Colombia. J Glob Antimicrob Resist. 2020 Sep;22:358-366. doi: 10.1016/j.jgar.2020.02.033.2213-7165https://hdl.handle.net/10495/3115310.1016/j.jgar.2020.02.0332213-7173ABSTRACT : Objectives: Carbapenem-resistant Gram-negative bacilli (CRGNB) have been reported in different wastewater treatment plants (WWTPs) throughout the world; however, few studies have described the antimicrobial resistance profile in different CRGNB throughout WWTPs, information that would identify points of selection of resistant bacteria. The objective of this work was to characterize the resistance profile of CRGNB harbouring blaKPC-2 from a Colombian WWTP. Methods: Six samples were taken from four points of a WWTP. CRGNB were selected in chromID1 CARBA and identified by 16S rRNA. Carbapenemases were determined by polymerase chain reaction (PCR), and susceptibility was assessed using VITEK2. Results: One hundred and forty-two CRGNB harbouring blaKPC-2 were detected: 41% corresponded to Aeromonas spp. (n = 58) and 59% to Enterobacteriaceae. To establish the resistance profile, 50% of the isolates were selected proportionally by family and sampling point (26 Aeromonadaceae and 45 Enterobacteriaceae). All Enterobacteriaceae showed resistance to carbapenems and penicillins + inhibitors, high percentages of resistance to ceftriaxone (88.9%), and ciprofloxacin (44.4%), and low resistance to other antibiotics (>30%). In Aeromonadaceae, 76.9% were resistant to ceftriaxone, 58% to carbapenems, and 65.4% to ciprofloxacin. Twenty-one resistance profiles were observed, the most common of which were resistant to penicillins + inhibitor, cephalosporins (third to fourth generation), and carbapenems (19%). The percentage of multidrug resistance was 91% and was similar at all points of the WWTP. Conclusions: The high frequency of multidrug resistance and great diversity of resistance profiles observed throughout the WWTP is of concern, and shows the role of WWTP as a reservoir and dissemination source of antimicrobial resistance to water sources.COL01261319application/pdfengElsevierGrupo de Investigación en Microbiología Básica y Aplicada-MicrobaÁmsterdam, Países Bajosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Multidrug resistance and diversity of resistance profiles in carbapenem-resistant Gram-negative bacilli throughout a wastewater treatment plant in ColombiaPlantas de Tratamiento de Aguas ResidualesWastewater Treatment PlantsResistencia a Múltiples MedicamentosDrug Resistance, MultipleCarbapenémicosCarbapenemsEnterobacteriaceaeAeromonadaceaeARN Ribosómico 16SRNA, Ribosomal, 16SPurificación del AguaWater PurificationJ. Glob. Antimicrob. Resist.Journal of Global Antimicrobial Resistance35836622ORIGINALRodriguezErika_2020_MultidrugResistance.pdfRodriguezErika_2020_MultidrugResistance.pdfArtículo de investigaciónapplication/pdf1571708https://bibliotecadigital.udea.edu.co/bitstream/10495/31153/1/RodriguezErika_2020_MultidrugResistance.pdf22e1dde3f5df06ec167da5d2bb8b1cf2MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstream/10495/31153/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/31153/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/31153oai:bibliotecadigital.udea.edu.co:10495/311532022-10-08 16:33:01.579Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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