DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species

ABSTRACT: The diversity of Trichoderma (Hypocreales, Ascomycota) colonizing leaf litter as well as the rhizosphere of Garcinia macrophylla (Clusiaceae) was investigated in primary and secondary rain forests in Colombian Amazonia. DNA barcoding of 107 strains based on the internal transcribed spacers...

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Autores:
López Quintero, Carlos Alberto
Atanasova, Lea
Franco Molano, Ana Esperanza
Gams, Walter
Komon Zelazowska, Monika
Theelen, Bart
Muller, Wally H.
Boekhout, Teun
Druzhinina, Irina
Tipo de recurso:
Article of investigation
Fecha de publicación:
2013
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/32710
Acceso en línea:
https://hdl.handle.net/10495/32710
Palabra clave:
Biodiversidad
Biodiversity
Hypocrea
Rhizosphere
Rizosfera
Análisis por Conglomerados
Cluster Analysis
Código de Barras del ADN Taxonómico
DNA Barcoding, Taxonomic
ADN de Hongos
DNA, Fungal
Técnicas Microbiológicas
Microbiological Techniques
Datos de Secuencia Molecular
Molecular Sequence Data
Filogenia
Phylogeny
Trichoderma
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
id UDEA2_2d40e5d7bc4df19a764ee57985ff5bbc
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/32710
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species
title DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species
spellingShingle DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species
Biodiversidad
Biodiversity
Hypocrea
Rhizosphere
Rizosfera
Análisis por Conglomerados
Cluster Analysis
Código de Barras del ADN Taxonómico
DNA Barcoding, Taxonomic
ADN de Hongos
DNA, Fungal
Técnicas Microbiológicas
Microbiological Techniques
Datos de Secuencia Molecular
Molecular Sequence Data
Filogenia
Phylogeny
Trichoderma
title_short DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species
title_full DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species
title_fullStr DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species
title_full_unstemmed DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species
title_sort DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species
dc.creator.fl_str_mv López Quintero, Carlos Alberto
Atanasova, Lea
Franco Molano, Ana Esperanza
Gams, Walter
Komon Zelazowska, Monika
Theelen, Bart
Muller, Wally H.
Boekhout, Teun
Druzhinina, Irina
dc.contributor.author.none.fl_str_mv López Quintero, Carlos Alberto
Atanasova, Lea
Franco Molano, Ana Esperanza
Gams, Walter
Komon Zelazowska, Monika
Theelen, Bart
Muller, Wally H.
Boekhout, Teun
Druzhinina, Irina
dc.subject.decs.none.fl_str_mv Biodiversidad
Biodiversity
Hypocrea
Rhizosphere
Rizosfera
Análisis por Conglomerados
Cluster Analysis
Código de Barras del ADN Taxonómico
DNA Barcoding, Taxonomic
ADN de Hongos
DNA, Fungal
Técnicas Microbiológicas
Microbiological Techniques
Datos de Secuencia Molecular
Molecular Sequence Data
Filogenia
Phylogeny
Trichoderma
topic Biodiversidad
Biodiversity
Hypocrea
Rhizosphere
Rizosfera
Análisis por Conglomerados
Cluster Analysis
Código de Barras del ADN Taxonómico
DNA Barcoding, Taxonomic
ADN de Hongos
DNA, Fungal
Técnicas Microbiológicas
Microbiological Techniques
Datos de Secuencia Molecular
Molecular Sequence Data
Filogenia
Phylogeny
Trichoderma
description ABSTRACT: The diversity of Trichoderma (Hypocreales, Ascomycota) colonizing leaf litter as well as the rhizosphere of Garcinia macrophylla (Clusiaceae) was investigated in primary and secondary rain forests in Colombian Amazonia. DNA barcoding of 107 strains based on the internal transcribed spacers 1 and 2 (ITS1 and 2) of the ribosomal RNA gene cluster and the partial sequence of the translation elongation factor 1 alpha (tef1) gene revealed that the diversity of Trichoderma was dominated (71 %) by three common cosmopolitan species, namely Trichoderma harzianum sensu lato (41 %), Trichoderma spirale (17 %) and Trichoderma koningiopsis (13 %). Four ITS 1 and 2 phylotypes (13 strains) could not be identified with certainty. Multigene phylogenetic analysis and phenotype profiling of four strains with an ITS1 and 2 phylotype similar to Trichoderma strigosum revealed a new sister species of the latter that is described here as Trichoderma strigosellum sp. nov. Sequence similarity searches revealed that this species also occurs in soils of Malaysia and Cameroon, suggesting a pantropical distribution.
publishDate 2013
dc.date.issued.none.fl_str_mv 2013
dc.date.accessioned.none.fl_str_mv 2022-12-11T15:33:09Z
dc.date.available.none.fl_str_mv 2022-12-11T15:33:09Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv López, Carlos & Atanasova, Lea & Molano, Ana & Komon-Zelazowska, Monika & Theelen, B.J.F. & Muller, W.H. & Boekhout, Teun & Druzhinina, Irina. (2013). DNA barcoding survey of Trichoderma diversity in soil and litter of the Colombian lowland Amazonian rainforest reveals Trichoderma strigosellum Sp. Nov. and other species. Antonie van Leeuwenhoek. 104. 10.1007/s10482-013-9975-4.
dc.identifier.issn.none.fl_str_mv 0003-6072
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/10495/32710
dc.identifier.doi.none.fl_str_mv 10.1007/s10482-013-9975-4
dc.identifier.eissn.none.fl_str_mv 1572-9699
identifier_str_mv López, Carlos & Atanasova, Lea & Molano, Ana & Komon-Zelazowska, Monika & Theelen, B.J.F. & Muller, W.H. & Boekhout, Teun & Druzhinina, Irina. (2013). DNA barcoding survey of Trichoderma diversity in soil and litter of the Colombian lowland Amazonian rainforest reveals Trichoderma strigosellum Sp. Nov. and other species. Antonie van Leeuwenhoek. 104. 10.1007/s10482-013-9975-4.
0003-6072
10.1007/s10482-013-9975-4
1572-9699
url https://hdl.handle.net/10495/32710
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Antonie Van Leeuwenhoek
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
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dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/co/
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https://creativecommons.org/licenses/by/4.0/
dc.format.extent.spa.fl_str_mv 18
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Springer
dc.publisher.group.spa.fl_str_mv Taxonomía y Ecología de Hongos
dc.publisher.place.spa.fl_str_mv Wageningen, Países Bajos
institution Universidad de Antioquia
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spelling López Quintero, Carlos AlbertoAtanasova, LeaFranco Molano, Ana EsperanzaGams, WalterKomon Zelazowska, MonikaTheelen, BartMuller, Wally H.Boekhout, TeunDruzhinina, Irina2022-12-11T15:33:09Z2022-12-11T15:33:09Z2013López, Carlos & Atanasova, Lea & Molano, Ana & Komon-Zelazowska, Monika & Theelen, B.J.F. & Muller, W.H. & Boekhout, Teun & Druzhinina, Irina. (2013). DNA barcoding survey of Trichoderma diversity in soil and litter of the Colombian lowland Amazonian rainforest reveals Trichoderma strigosellum Sp. Nov. and other species. Antonie van Leeuwenhoek. 104. 10.1007/s10482-013-9975-4.0003-6072https://hdl.handle.net/10495/3271010.1007/s10482-013-9975-41572-9699ABSTRACT: The diversity of Trichoderma (Hypocreales, Ascomycota) colonizing leaf litter as well as the rhizosphere of Garcinia macrophylla (Clusiaceae) was investigated in primary and secondary rain forests in Colombian Amazonia. DNA barcoding of 107 strains based on the internal transcribed spacers 1 and 2 (ITS1 and 2) of the ribosomal RNA gene cluster and the partial sequence of the translation elongation factor 1 alpha (tef1) gene revealed that the diversity of Trichoderma was dominated (71 %) by three common cosmopolitan species, namely Trichoderma harzianum sensu lato (41 %), Trichoderma spirale (17 %) and Trichoderma koningiopsis (13 %). Four ITS 1 and 2 phylotypes (13 strains) could not be identified with certainty. Multigene phylogenetic analysis and phenotype profiling of four strains with an ITS1 and 2 phylotype similar to Trichoderma strigosum revealed a new sister species of the latter that is described here as Trichoderma strigosellum sp. nov. Sequence similarity searches revealed that this species also occurs in soils of Malaysia and Cameroon, suggesting a pantropical distribution.COL001176918application/pdfengSpringerTaxonomía y Ecología de HongosWageningen, Países Bajosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other speciesBiodiversidadBiodiversityHypocreaRhizosphereRizosferaAnálisis por ConglomeradosCluster AnalysisCódigo de Barras del ADN TaxonómicoDNA Barcoding, TaxonomicADN de HongosDNA, FungalTécnicas MicrobiológicasMicrobiological TechniquesDatos de Secuencia MolecularMolecular Sequence DataFilogeniaPhylogenyTrichodermaAntonie Van LeeuwenhoekAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology6576741045ORIGINALLopezCarlos_2013_DnaBarcodingSurvey.pdfLopezCarlos_2013_DnaBarcodingSurvey.pdfArtículo de investigaciónapplication/pdf819353https://bibliotecadigital.udea.edu.co/bitstream/10495/32710/1/LopezCarlos_2013_DnaBarcodingSurvey.pdf1a9600ef01767a6a89ab6cc1a3c1e2b5MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstream/10495/32710/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/32710/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/32710oai:bibliotecadigital.udea.edu.co:10495/327102022-12-11 10:33:09.532Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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