DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species
ABSTRACT: The diversity of Trichoderma (Hypocreales, Ascomycota) colonizing leaf litter as well as the rhizosphere of Garcinia macrophylla (Clusiaceae) was investigated in primary and secondary rain forests in Colombian Amazonia. DNA barcoding of 107 strains based on the internal transcribed spacers...
- Autores:
-
López Quintero, Carlos Alberto
Atanasova, Lea
Franco Molano, Ana Esperanza
Gams, Walter
Komon Zelazowska, Monika
Theelen, Bart
Muller, Wally H.
Boekhout, Teun
Druzhinina, Irina
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2013
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/32710
- Acceso en línea:
- https://hdl.handle.net/10495/32710
- Palabra clave:
- Biodiversidad
Biodiversity
Hypocrea
Rhizosphere
Rizosfera
Análisis por Conglomerados
Cluster Analysis
Código de Barras del ADN Taxonómico
DNA Barcoding, Taxonomic
ADN de Hongos
DNA, Fungal
Técnicas Microbiológicas
Microbiological Techniques
Datos de Secuencia Molecular
Molecular Sequence Data
Filogenia
Phylogeny
Trichoderma
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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|
dc.title.spa.fl_str_mv |
DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species |
title |
DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species |
spellingShingle |
DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species Biodiversidad Biodiversity Hypocrea Rhizosphere Rizosfera Análisis por Conglomerados Cluster Analysis Código de Barras del ADN Taxonómico DNA Barcoding, Taxonomic ADN de Hongos DNA, Fungal Técnicas Microbiológicas Microbiological Techniques Datos de Secuencia Molecular Molecular Sequence Data Filogenia Phylogeny Trichoderma |
title_short |
DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species |
title_full |
DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species |
title_fullStr |
DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species |
title_full_unstemmed |
DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species |
title_sort |
DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species |
dc.creator.fl_str_mv |
López Quintero, Carlos Alberto Atanasova, Lea Franco Molano, Ana Esperanza Gams, Walter Komon Zelazowska, Monika Theelen, Bart Muller, Wally H. Boekhout, Teun Druzhinina, Irina |
dc.contributor.author.none.fl_str_mv |
López Quintero, Carlos Alberto Atanasova, Lea Franco Molano, Ana Esperanza Gams, Walter Komon Zelazowska, Monika Theelen, Bart Muller, Wally H. Boekhout, Teun Druzhinina, Irina |
dc.subject.decs.none.fl_str_mv |
Biodiversidad Biodiversity Hypocrea Rhizosphere Rizosfera Análisis por Conglomerados Cluster Analysis Código de Barras del ADN Taxonómico DNA Barcoding, Taxonomic ADN de Hongos DNA, Fungal Técnicas Microbiológicas Microbiological Techniques Datos de Secuencia Molecular Molecular Sequence Data Filogenia Phylogeny Trichoderma |
topic |
Biodiversidad Biodiversity Hypocrea Rhizosphere Rizosfera Análisis por Conglomerados Cluster Analysis Código de Barras del ADN Taxonómico DNA Barcoding, Taxonomic ADN de Hongos DNA, Fungal Técnicas Microbiológicas Microbiological Techniques Datos de Secuencia Molecular Molecular Sequence Data Filogenia Phylogeny Trichoderma |
description |
ABSTRACT: The diversity of Trichoderma (Hypocreales, Ascomycota) colonizing leaf litter as well as the rhizosphere of Garcinia macrophylla (Clusiaceae) was investigated in primary and secondary rain forests in Colombian Amazonia. DNA barcoding of 107 strains based on the internal transcribed spacers 1 and 2 (ITS1 and 2) of the ribosomal RNA gene cluster and the partial sequence of the translation elongation factor 1 alpha (tef1) gene revealed that the diversity of Trichoderma was dominated (71 %) by three common cosmopolitan species, namely Trichoderma harzianum sensu lato (41 %), Trichoderma spirale (17 %) and Trichoderma koningiopsis (13 %). Four ITS 1 and 2 phylotypes (13 strains) could not be identified with certainty. Multigene phylogenetic analysis and phenotype profiling of four strains with an ITS1 and 2 phylotype similar to Trichoderma strigosum revealed a new sister species of the latter that is described here as Trichoderma strigosellum sp. nov. Sequence similarity searches revealed that this species also occurs in soils of Malaysia and Cameroon, suggesting a pantropical distribution. |
publishDate |
2013 |
dc.date.issued.none.fl_str_mv |
2013 |
dc.date.accessioned.none.fl_str_mv |
2022-12-11T15:33:09Z |
dc.date.available.none.fl_str_mv |
2022-12-11T15:33:09Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.citation.spa.fl_str_mv |
López, Carlos & Atanasova, Lea & Molano, Ana & Komon-Zelazowska, Monika & Theelen, B.J.F. & Muller, W.H. & Boekhout, Teun & Druzhinina, Irina. (2013). DNA barcoding survey of Trichoderma diversity in soil and litter of the Colombian lowland Amazonian rainforest reveals Trichoderma strigosellum Sp. Nov. and other species. Antonie van Leeuwenhoek. 104. 10.1007/s10482-013-9975-4. |
dc.identifier.issn.none.fl_str_mv |
0003-6072 |
dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/10495/32710 |
dc.identifier.doi.none.fl_str_mv |
10.1007/s10482-013-9975-4 |
dc.identifier.eissn.none.fl_str_mv |
1572-9699 |
identifier_str_mv |
López, Carlos & Atanasova, Lea & Molano, Ana & Komon-Zelazowska, Monika & Theelen, B.J.F. & Muller, W.H. & Boekhout, Teun & Druzhinina, Irina. (2013). DNA barcoding survey of Trichoderma diversity in soil and litter of the Colombian lowland Amazonian rainforest reveals Trichoderma strigosellum Sp. Nov. and other species. Antonie van Leeuwenhoek. 104. 10.1007/s10482-013-9975-4. 0003-6072 10.1007/s10482-013-9975-4 1572-9699 |
url |
https://hdl.handle.net/10495/32710 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Antonie Van Leeuwenhoek |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.uri.*.fl_str_mv |
http://creativecommons.org/licenses/by/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
dc.format.extent.spa.fl_str_mv |
18 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
Springer |
dc.publisher.group.spa.fl_str_mv |
Taxonomía y Ecología de Hongos |
dc.publisher.place.spa.fl_str_mv |
Wageningen, Países Bajos |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstream/10495/32710/1/LopezCarlos_2013_DnaBarcodingSurvey.pdf https://bibliotecadigital.udea.edu.co/bitstream/10495/32710/2/license_rdf https://bibliotecadigital.udea.edu.co/bitstream/10495/32710/3/license.txt |
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bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad de Antioquia |
repository.mail.fl_str_mv |
andres.perez@udea.edu.co |
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1812173107582992384 |
spelling |
López Quintero, Carlos AlbertoAtanasova, LeaFranco Molano, Ana EsperanzaGams, WalterKomon Zelazowska, MonikaTheelen, BartMuller, Wally H.Boekhout, TeunDruzhinina, Irina2022-12-11T15:33:09Z2022-12-11T15:33:09Z2013López, Carlos & Atanasova, Lea & Molano, Ana & Komon-Zelazowska, Monika & Theelen, B.J.F. & Muller, W.H. & Boekhout, Teun & Druzhinina, Irina. (2013). DNA barcoding survey of Trichoderma diversity in soil and litter of the Colombian lowland Amazonian rainforest reveals Trichoderma strigosellum Sp. Nov. and other species. Antonie van Leeuwenhoek. 104. 10.1007/s10482-013-9975-4.0003-6072https://hdl.handle.net/10495/3271010.1007/s10482-013-9975-41572-9699ABSTRACT: The diversity of Trichoderma (Hypocreales, Ascomycota) colonizing leaf litter as well as the rhizosphere of Garcinia macrophylla (Clusiaceae) was investigated in primary and secondary rain forests in Colombian Amazonia. DNA barcoding of 107 strains based on the internal transcribed spacers 1 and 2 (ITS1 and 2) of the ribosomal RNA gene cluster and the partial sequence of the translation elongation factor 1 alpha (tef1) gene revealed that the diversity of Trichoderma was dominated (71 %) by three common cosmopolitan species, namely Trichoderma harzianum sensu lato (41 %), Trichoderma spirale (17 %) and Trichoderma koningiopsis (13 %). Four ITS 1 and 2 phylotypes (13 strains) could not be identified with certainty. Multigene phylogenetic analysis and phenotype profiling of four strains with an ITS1 and 2 phylotype similar to Trichoderma strigosum revealed a new sister species of the latter that is described here as Trichoderma strigosellum sp. nov. Sequence similarity searches revealed that this species also occurs in soils of Malaysia and Cameroon, suggesting a pantropical distribution.COL001176918application/pdfengSpringerTaxonomía y Ecología de HongosWageningen, Países Bajosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other speciesBiodiversidadBiodiversityHypocreaRhizosphereRizosferaAnálisis por ConglomeradosCluster AnalysisCódigo de Barras del ADN TaxonómicoDNA Barcoding, TaxonomicADN de HongosDNA, FungalTécnicas MicrobiológicasMicrobiological TechniquesDatos de Secuencia MolecularMolecular Sequence DataFilogeniaPhylogenyTrichodermaAntonie Van LeeuwenhoekAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology6576741045ORIGINALLopezCarlos_2013_DnaBarcodingSurvey.pdfLopezCarlos_2013_DnaBarcodingSurvey.pdfArtículo de investigaciónapplication/pdf819353https://bibliotecadigital.udea.edu.co/bitstream/10495/32710/1/LopezCarlos_2013_DnaBarcodingSurvey.pdf1a9600ef01767a6a89ab6cc1a3c1e2b5MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927https://bibliotecadigital.udea.edu.co/bitstream/10495/32710/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/32710/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/32710oai:bibliotecadigital.udea.edu.co:10495/327102022-12-11 10:33:09.532Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.coTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo= |