DNA barcoding survey of Trichoderma diversity in soil and litter of the Amazonian rainforest reveals Trichoderma strigosellun sp, nov. and other species

ABSTRACT: The diversity of Trichoderma (Hypocreales, Ascomycota) colonizing leaf litter as well as the rhizosphere of Garcinia macrophylla (Clusiaceae) was investigated in primary and secondary rain forests in Colombian Amazonia. DNA barcoding of 107 strains based on the internal transcribed spacers...

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Autores:
López Quintero, Carlos Alberto
Atanasova, Lea
Franco Molano, Ana Esperanza
Gams, Walter
Komon Zelazowska, Monika
Theelen, Bart
Muller, Wally H.
Boekhout, Teun
Druzhinina, Irina
Tipo de recurso:
Article of investigation
Fecha de publicación:
2013
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/32710
Acceso en línea:
https://hdl.handle.net/10495/32710
Palabra clave:
Biodiversidad
Biodiversity
Hypocrea
Rhizosphere
Rizosfera
Análisis por Conglomerados
Cluster Analysis
Código de Barras del ADN Taxonómico
DNA Barcoding, Taxonomic
ADN de Hongos
DNA, Fungal
Técnicas Microbiológicas
Microbiological Techniques
Datos de Secuencia Molecular
Molecular Sequence Data
Filogenia
Phylogeny
Trichoderma
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
Description
Summary:ABSTRACT: The diversity of Trichoderma (Hypocreales, Ascomycota) colonizing leaf litter as well as the rhizosphere of Garcinia macrophylla (Clusiaceae) was investigated in primary and secondary rain forests in Colombian Amazonia. DNA barcoding of 107 strains based on the internal transcribed spacers 1 and 2 (ITS1 and 2) of the ribosomal RNA gene cluster and the partial sequence of the translation elongation factor 1 alpha (tef1) gene revealed that the diversity of Trichoderma was dominated (71 %) by three common cosmopolitan species, namely Trichoderma harzianum sensu lato (41 %), Trichoderma spirale (17 %) and Trichoderma koningiopsis (13 %). Four ITS 1 and 2 phylotypes (13 strains) could not be identified with certainty. Multigene phylogenetic analysis and phenotype profiling of four strains with an ITS1 and 2 phylotype similar to Trichoderma strigosum revealed a new sister species of the latter that is described here as Trichoderma strigosellum sp. nov. Sequence similarity searches revealed that this species also occurs in soils of Malaysia and Cameroon, suggesting a pantropical distribution.