La estimulación de TLR, receptores tipo NOD y dectina-1 en neutrófilos humanos induce la producción de citocinas proinflamatorias

ABSTRACT: To evaluate the expression and function of pattern recognition receptors such as Toll-like receptors, RIG-I/MDA5, NOD-like receptors, Dectin-1 and adaptor proteins, in human neutrophils. Methods: Neutrophils from peripheral blood of healthy individuals were purified and cultured in RPMI-1640, in the presence or absence of specific agonists of the receptor of interest. The expression of pattern recognition receptors was determined by RT-PCR and the secretion of proinflammatory cytokines, by ELISA. Results: We observed that neutrophils express diverse patterns recognition receptors and adaptor molecules. Stimulation of TLR4, TLR5 and TLR7/8 induces the production of IL-1β and IL-6, and activation of Dectin-1 leads to secretion of high levels of TNF-α, but low levels of IL-1β and IL-6. Conclusion: Neutrophils express a large number of pattern recognition receptors and their activation leads to the expression of proinflammatory cytokines.

• Autores: Martínez Moreno, Jahnnyer Anilio; Hernández López, Juan Carlos; Urcuqui Inchima, Silvio
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2014
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: spa
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/12886
• Acceso en línea: http://hdl.handle.net/10495/12886
• Palabra clave: Citocinas; Inflamación; Neutrófilos; Proteínas Adaptadoras de Señalización NOD; Receptores de Reconocimiento de Patrones; Receptores Toll-Like
• Rights: openAccess
• License: Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)