Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution
ABSTRACT : Silk from Bombyx mori has two main proteins: fibroin and sericin. Fibroin is a protein that exhibits good biocompatibility and high surface reactivity, desirable properties for many biomedical applications. Sericin is related with adverse immune response in some medical uses. Therefore, i...
- Autores:
-
Echeverri Correa, Estefanía
Grajales Lopera, David Orlando
Gutiérrez Restrepo, Santiago
Ossa Orozco, Claudia Patricia
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2020
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/24955
- Acceso en línea:
- http://hdl.handle.net/10495/24955
https://revistas.udea.edu.co/index.php/ingenieria/article/view/336655
- Palabra clave:
- Fibroínas
Fibroins
Diálisis
Dialysis
Ingeniería de Tejidos
Tissue Engineering
Membrana
Membranes
Proceso de desgomado
http://aims.fao.org/aos/agrovoc/c_28457
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| dc.title.alternative.spa.fl_str_mv |
Separación desericina/fibroína deseda del Bombyx moriyremoción asequible desales |
| title |
Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| spellingShingle |
Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution Fibroínas Fibroins Diálisis Dialysis Ingeniería de Tejidos Tissue Engineering Membrana Membranes Proceso de desgomado http://aims.fao.org/aos/agrovoc/c_28457 |
| title_short |
Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| title_full |
Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| title_fullStr |
Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| title_full_unstemmed |
Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| title_sort |
Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution |
| dc.creator.fl_str_mv |
Echeverri Correa, Estefanía Grajales Lopera, David Orlando Gutiérrez Restrepo, Santiago Ossa Orozco, Claudia Patricia |
| dc.contributor.author.none.fl_str_mv |
Echeverri Correa, Estefanía Grajales Lopera, David Orlando Gutiérrez Restrepo, Santiago Ossa Orozco, Claudia Patricia |
| dc.subject.decs.none.fl_str_mv |
Fibroínas Fibroins Diálisis Dialysis Ingeniería de Tejidos Tissue Engineering |
| topic |
Fibroínas Fibroins Diálisis Dialysis Ingeniería de Tejidos Tissue Engineering Membrana Membranes Proceso de desgomado http://aims.fao.org/aos/agrovoc/c_28457 |
| dc.subject.agrovoc.none.fl_str_mv |
Membrana Membranes |
| dc.subject.proposal.spa.fl_str_mv |
Proceso de desgomado |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_28457 |
| description |
ABSTRACT : Silk from Bombyx mori has two main proteins: fibroin and sericin. Fibroin is a protein that exhibits good biocompatibility and high surface reactivity, desirable properties for many biomedical applications. Sericin is related with adverse immune response in some medical uses. Therefore, its removal (degumming) is desirable in almost all fields, including the textile industry and biological applications. In this study, three degumming methods (distilled water, liquid neutral detergent and sodium carbonate solution) were evaluated using Raman spectroscopy. Degummed silk treated with sodium carbonate solution exhibits a pattern consistent with previous studies for sericin-free fibroin, and significant differences with the untreated silk pattern. Also, degummed fibroin fibers were dissolved in a calcium chloride solution, in pursuit of a more versatile material. Additionally, different porous membranes of dialysis tubes were tested to remove residual salts, and were compared by conductivity measurements and EDS analysis, identifying good performance for an affordable food cellulose membrane. The results showed that it is possible to obtain fibroin with adequate chemical properties, using low-cost process and membrane of dialysis tubes. |
| publishDate |
2020 |
| dc.date.issued.none.fl_str_mv |
2020 |
| dc.date.accessioned.none.fl_str_mv |
2021-12-10T21:03:35Z |
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2021-12-10T21:03:35Z |
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info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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Echeverri-Correa E., Grajales-Lopera D. O., Gutiérrez-Restrepo S., and Ossa-Orozco C. P., “Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution”, Rev.Fac.Ing.Univ.Antioquia, no. 94, pp. 97-101, Oct. 2020. |
| dc.identifier.issn.none.fl_str_mv |
0120-6230 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/24955 |
| dc.identifier.doi.none.fl_str_mv |
10.17533/udea.redin.20190731 |
| dc.identifier.eissn.none.fl_str_mv |
2422-2844 |
| dc.identifier.url.spa.fl_str_mv |
https://revistas.udea.edu.co/index.php/ingenieria/article/view/336655 |
| identifier_str_mv |
Echeverri-Correa E., Grajales-Lopera D. O., Gutiérrez-Restrepo S., and Ossa-Orozco C. P., “Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution”, Rev.Fac.Ing.Univ.Antioquia, no. 94, pp. 97-101, Oct. 2020. 0120-6230 10.17533/udea.redin.20190731 2422-2844 |
| url |
http://hdl.handle.net/10495/24955 https://revistas.udea.edu.co/index.php/ingenieria/article/view/336655 |
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eng |
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eng |
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Rev. Fac. Ing. Univ. Antioquia |
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openAccess |
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Universidad de Antioquia, Facultad de Ingeniería |
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Grupo de Investigación en Biomateriales |
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Medellín, Colombia |
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Universidad de Antioquia |
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Echeverri Correa, EstefaníaGrajales Lopera, David OrlandoGutiérrez Restrepo, SantiagoOssa Orozco, Claudia Patricia2021-12-10T21:03:35Z2021-12-10T21:03:35Z2020Echeverri-Correa E., Grajales-Lopera D. O., Gutiérrez-Restrepo S., and Ossa-Orozco C. P., “Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solution”, Rev.Fac.Ing.Univ.Antioquia, no. 94, pp. 97-101, Oct. 2020.0120-6230http://hdl.handle.net/10495/2495510.17533/udea.redin.201907312422-2844https://revistas.udea.edu.co/index.php/ingenieria/article/view/336655ABSTRACT : Silk from Bombyx mori has two main proteins: fibroin and sericin. Fibroin is a protein that exhibits good biocompatibility and high surface reactivity, desirable properties for many biomedical applications. Sericin is related with adverse immune response in some medical uses. Therefore, its removal (degumming) is desirable in almost all fields, including the textile industry and biological applications. In this study, three degumming methods (distilled water, liquid neutral detergent and sodium carbonate solution) were evaluated using Raman spectroscopy. Degummed silk treated with sodium carbonate solution exhibits a pattern consistent with previous studies for sericin-free fibroin, and significant differences with the untreated silk pattern. Also, degummed fibroin fibers were dissolved in a calcium chloride solution, in pursuit of a more versatile material. Additionally, different porous membranes of dialysis tubes were tested to remove residual salts, and were compared by conductivity measurements and EDS analysis, identifying good performance for an affordable food cellulose membrane. The results showed that it is possible to obtain fibroin with adequate chemical properties, using low-cost process and membrane of dialysis tubes.RESUMEN : La seda del Bombyx mori tiene dos proteínas principales: fibroína y sericina. La fibroína es una proteína que presenta buena biocompatibilidad y alta reactividad superficial, propiedades deseables para varias aplicaciones biomédicas. La sericina está relacionada con respuestas inmunes adversas en algunos usos médicos. Además, su remoción (desgomado) es deseable en casi todos los campos, incluyendo la industria textil y aplicaciones biológicas. En el presente estudio se evaluaron, mediante espectroscopía Raman, tres métodos de desgomado (agua destilada, detergente líquido neutro y solución de carbonato de sodio). La seda desgomada con solución de carbonato de sodio muestra un patrón consistente con estudios previos de fibroína libre de sericina, y exhibe diferencias significativas respecto al patrón de la seda no tratada. Adicionalmente las fibras de fibroína desgomada se disolvieron en solución de cloruro de calcio, buscando un material más versátil. Además, diferentes membranas porosas fueron probadas para la remoción de sales residuales y comparadas, mediante medición de la conductividad y análisis EDS, identificando buen desempeño de la membrana de celulosa para alimentos que, además, es económica. Los resultados mostraron que es posible obtener fibroína con propiedades químicas adecuadas, usando procesos y membranas de diálisis de bajo costo.COL00550495application/pdfengUniversidad de Antioquia, Facultad de IngenieríaGrupo de Investigación en BiomaterialesMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Effective sericin-fibroin separation from Bombyx mori silkworms fibers and low-cost salt removal from fibroin solutionSeparación desericina/fibroína deseda del Bombyx moriyremoción asequible desalesFibroínasFibroinsDiálisisDialysisIngeniería de TejidosTissue EngineeringMembranaMembranesProceso de desgomadohttp://aims.fao.org/aos/agrovoc/c_28457Rev. Fac. Ing. Univ. AntioquiaRevista Facultad de Ingeniería Universidad de Antioquia9710194ORIGINALEcheverriEstefania_2020_EffectiveSericin-fibroinSeparation.pdfEcheverriEstefania_2020_EffectiveSericin-fibroinSeparation.pdfArtículo de investigaciónapplication/pdf146593http://bibliotecadigital.udea.edu.co/bitstream/10495/24955/1/EcheverriEstefania_2020_EffectiveSericin-fibroinSeparation.pdf29657b6238a1b6420d00983d67ca437eMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051http://bibliotecadigital.udea.edu.co/bitstream/10495/24955/2/license_rdfe2060682c9c70d4d30c83c51448f4eedMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/24955/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/24955oai:bibliotecadigital.udea.edu.co:10495/249552021-12-10 16:03:35.703Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |