Diversity of Toxigenic Bacillus cereus in Powder Milk
ABSTRACT : Bacillus cereus is a bacterium commonly isolated from food and produces multiple toxins such as hemolysin BL (HBL), non-hemolytic enterotoxin (NHE), cytotoxin K (CytK) and emetic toxin or cereulide that cause human food poisoning. The detection of B. cereus by traditional plating does not...
- Autores:
-
Sánchez Chica, Jennifer
Correa Ochoa, Margarita María
Aceves Díez, Angel Emilio
Castañeda Sandoval, Laura Margarita
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2014
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/26453
- Acceso en línea:
- http://hdl.handle.net/10495/26453
https://revistas.unal.edu.co/index.php/refame/issue/view/5130
- Palabra clave:
- Enterotoxins
Enterotoxinas
Reacción en Cadena de la Polimerasa Multiplex
Multiplex Polymerase Chain Reaction
Microorganismos patógenos
Micro-organisms, pathogenic
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by-nc-sa/2.5/co/
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| dc.title.spa.fl_str_mv |
Diversity of Toxigenic Bacillus cereus in Powder Milk |
| dc.title.alternative.spa.fl_str_mv |
Diversidad de Bacillus cereus Toxigénicos en Leche en Polvo |
| title |
Diversity of Toxigenic Bacillus cereus in Powder Milk |
| spellingShingle |
Diversity of Toxigenic Bacillus cereus in Powder Milk Enterotoxins Enterotoxinas Reacción en Cadena de la Polimerasa Multiplex Multiplex Polymerase Chain Reaction Microorganismos patógenos Micro-organisms, pathogenic |
| title_short |
Diversity of Toxigenic Bacillus cereus in Powder Milk |
| title_full |
Diversity of Toxigenic Bacillus cereus in Powder Milk |
| title_fullStr |
Diversity of Toxigenic Bacillus cereus in Powder Milk |
| title_full_unstemmed |
Diversity of Toxigenic Bacillus cereus in Powder Milk |
| title_sort |
Diversity of Toxigenic Bacillus cereus in Powder Milk |
| dc.creator.fl_str_mv |
Sánchez Chica, Jennifer Correa Ochoa, Margarita María Aceves Díez, Angel Emilio Castañeda Sandoval, Laura Margarita |
| dc.contributor.author.none.fl_str_mv |
Sánchez Chica, Jennifer Correa Ochoa, Margarita María Aceves Díez, Angel Emilio Castañeda Sandoval, Laura Margarita |
| dc.subject.decs.none.fl_str_mv |
Enterotoxins Enterotoxinas Reacción en Cadena de la Polimerasa Multiplex Multiplex Polymerase Chain Reaction |
| topic |
Enterotoxins Enterotoxinas Reacción en Cadena de la Polimerasa Multiplex Multiplex Polymerase Chain Reaction Microorganismos patógenos Micro-organisms, pathogenic |
| dc.subject.lemb.none.fl_str_mv |
Microorganismos patógenos Micro-organisms, pathogenic |
| description |
ABSTRACT : Bacillus cereus is a bacterium commonly isolated from food and produces multiple toxins such as hemolysin BL (HBL), non-hemolytic enterotoxin (NHE), cytotoxin K (CytK) and emetic toxin or cereulide that cause human food poisoning. The detection of B. cereus by traditional plating does not allow detecting the toxic potential and toxigenic diversity of the strains in food. Therefore, in this work, a multiplex PCR to direct detection of toxigenic B. cereus from powder milk samples collected in Medellín, Colombia, was applied. According to the pattern of toxic genes found in the samples, was possible to establish five different toxigenic consortia: I (nheA, hblC), II (I (nheA, hblC, cytK), III (hblC), IV (hblC, cytK) and V (nheA, cesB). These results suggest a wide diversity of toxigenic B. cereus in the evaluated samples. |
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2014 |
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2014 |
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2022-03-08T16:30:21Z |
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2022-03-08T16:30:21Z |
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Artículo de investigación |
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0304-2847 |
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http://hdl.handle.net/10495/26453 |
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2248-7026 |
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https://revistas.unal.edu.co/index.php/refame/issue/view/5130 |
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0304-2847 2248-7026 |
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http://hdl.handle.net/10495/26453 https://revistas.unal.edu.co/index.php/refame/issue/view/5130 |
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eng |
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eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Fac. Nac. Agron. Medellín |
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http://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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Universidad Nacional de Colombia, Facultad de Ciencias Agrarias |
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BIOMICRO Microbiología Molecular |
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Medellín, Colombia |
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Universidad de Antioquia |
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http://bibliotecadigital.udea.edu.co/bitstream/10495/26453/1/SanchezJennifer_2014_DiversityToxigenicBacillus.pdf http://bibliotecadigital.udea.edu.co/bitstream/10495/26453/2/license_rdf http://bibliotecadigital.udea.edu.co/bitstream/10495/26453/3/license.txt |
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andres.perez@udea.edu.co |
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Sánchez Chica, JenniferCorrea Ochoa, Margarita MaríaAceves Díez, Angel EmilioCastañeda Sandoval, Laura Margarita2022-03-08T16:30:21Z2022-03-08T16:30:21Z20140304-2847http://hdl.handle.net/10495/264532248-7026https://revistas.unal.edu.co/index.php/refame/issue/view/5130ABSTRACT : Bacillus cereus is a bacterium commonly isolated from food and produces multiple toxins such as hemolysin BL (HBL), non-hemolytic enterotoxin (NHE), cytotoxin K (CytK) and emetic toxin or cereulide that cause human food poisoning. The detection of B. cereus by traditional plating does not allow detecting the toxic potential and toxigenic diversity of the strains in food. Therefore, in this work, a multiplex PCR to direct detection of toxigenic B. cereus from powder milk samples collected in Medellín, Colombia, was applied. According to the pattern of toxic genes found in the samples, was possible to establish five different toxigenic consortia: I (nheA, hblC), II (I (nheA, hblC, cytK), III (hblC), IV (hblC, cytK) and V (nheA, cesB). These results suggest a wide diversity of toxigenic B. cereus in the evaluated samples.RESUMEN : Bacillus cereus es una bacteria comúnmente aislada de alimentos, la cual produce toxinas como la hemolisina BL (HBL), enterotoxina no hemolítica (NHE), citotoxina K y toxina emética o cereúlida, que causan intoxicaciones alimentarias al hombre. La detección de B. cereus mediante pruebas tradicionales de cultivo en placa no permite determinar el potencial tóxico y diversidad toxigénica de las cepas; por lo cual, en este trabajo se aplicó una PCR múltiple que permitió la detección directa de B. cereus toxigénicos en muestras de leche en polvo colectadas en Medellín, Colombia. De acuerdo a los patrones de genes tóxicos encontrados en las muestras, fue posible establecer cinco consorcios toxigénicos diferentes: I (nheA, hblC), II (I (nheA, hblC, cytK), III (hblC), IV (hblC, cytK) y V (nheA, cesB). Estos resultados sugieren una amplia diversidad de B. cereus toxigénicos en las muestras evaluadas.COL0111863COL00137463application/pdfengUniversidad Nacional de Colombia, Facultad de Ciencias AgrariasBIOMICROMicrobiología MolecularMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Diversity of Toxigenic Bacillus cereus in Powder MilkDiversidad de Bacillus cereus Toxigénicos en Leche en PolvoEnterotoxinsEnterotoxinasReacción en Cadena de la Polimerasa MultiplexMultiplex Polymerase Chain ReactionMicroorganismos patógenosMicro-organisms, pathogenicRev. Fac. Nac. Agron. MedellínRevista Facultad Nacional de Agronomía Medellín85185367Suplemento 2ORIGINALSanchezJennifer_2014_DiversityToxigenicBacillus.pdfSanchezJennifer_2014_DiversityToxigenicBacillus.pdfArtículo de investigaciónapplication/pdf193525http://bibliotecadigital.udea.edu.co/bitstream/10495/26453/1/SanchezJennifer_2014_DiversityToxigenicBacillus.pdfa04dcf77b241ec2fe56c7ec6b276cd1cMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-81051http://bibliotecadigital.udea.edu.co/bitstream/10495/26453/2/license_rdfe2060682c9c70d4d30c83c51448f4eedMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/26453/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5310495/26453oai:bibliotecadigital.udea.edu.co:10495/264532022-04-22 10:23:16.567Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |