Diversity of Toxigenic Bacillus cereus in Powder Milk

ABSTRACT : Bacillus cereus is a bacterium commonly isolated from food and produces multiple toxins such as hemolysin BL (HBL), non-hemolytic enterotoxin (NHE), cytotoxin K (CytK) and emetic toxin or cereulide that cause human food poisoning. The detection of B. cereus by traditional plating does not allow detecting the toxic potential and toxigenic diversity of the strains in food. Therefore, in this work, a multiplex PCR to direct detection of toxigenic B. cereus from powder milk samples collected in Medellín, Colombia, was applied. According to the pattern of toxic genes found in the samples, was possible to establish five different toxigenic consortia: I (nheA, hblC), II (I (nheA, hblC, cytK), III (hblC), IV (hblC, cytK) and V (nheA, cesB). These results suggest a wide diversity of toxigenic B. cereus in the evaluated samples.

• Autores: Sánchez Chica, Jennifer; Correa Ochoa, Margarita María; Aceves Díez, Angel Emilio; Castañeda Sandoval, Laura Margarita
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2014
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/26453
• Acceso en línea: http://hdl.handle.net/10495/26453; https://revistas.unal.edu.co/index.php/refame/issue/view/5130
• Palabra clave: Enterotoxins; Enterotoxinas; Reacción en Cadena de la Polimerasa Multiplex; Multiplex Polymerase Chain Reaction; Microorganismos patógenos; Micro-organisms, pathogenic
• Rights: openAccess
• License: http://creativecommons.org/licenses/by-nc-sa/2.5/co/