Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis
ABSTRACT: Extracts from a variety of fruit trees have been used for therapeutic applications for preventing oxidative stress associated to chronic diseases. Objective: To investigate the antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits of Passiflora...
- Autores:
-
Aguillón Osma, Johanny
Maldonado Celis, María Elena
Loango Chamorro, Nelsy
Arango Varela, Sandra Sulay
Landázuri, Patricia
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2013
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- spa
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/11380
- Acceso en línea:
- http://hdl.handle.net/10495/11380
- Palabra clave:
- Passiflora edulis
Antioxidant
Antiproliferative
Cytotoxic
- Rights
- openAccess
- License
- Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
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| dc.title.spa.fl_str_mv |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| dc.title.alternative.spa.fl_str_mv |
Actividad antioxidante y antiproliferativa de extractos etanólico y acuoso de las hojas y el jugo del fruto de Passiflora edulis |
| title |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| spellingShingle |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis Passiflora edulis Antioxidant Antiproliferative Cytotoxic |
| title_short |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| title_full |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| title_fullStr |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| title_full_unstemmed |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| title_sort |
Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis |
| dc.creator.fl_str_mv |
Aguillón Osma, Johanny Maldonado Celis, María Elena Loango Chamorro, Nelsy Arango Varela, Sandra Sulay Landázuri, Patricia |
| dc.contributor.author.none.fl_str_mv |
Aguillón Osma, Johanny Maldonado Celis, María Elena Loango Chamorro, Nelsy Arango Varela, Sandra Sulay Landázuri, Patricia |
| dc.subject.none.fl_str_mv |
Passiflora edulis Antioxidant Antiproliferative Cytotoxic |
| topic |
Passiflora edulis Antioxidant Antiproliferative Cytotoxic |
| description |
ABSTRACT: Extracts from a variety of fruit trees have been used for therapeutic applications for preventing oxidative stress associated to chronic diseases. Objective: To investigate the antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits of Passiflora edulis. Materials and methods: A preliminary phytochemical screening was performed; antioxidant activity was evaluated through DPPH assay, the scavenging activity for hydroxyl radical, antihemolytic activity and total phenolic content; cytotoxic and antiproliferative activities were evaluated by MTT and sulforhodamine B assays respectively in colon adenocarcinoma SW480 cells and their metastatic-derived SW620 cells. Results: Phytochemical analyses revealed presence of tannins, flavonoids and cardiotonic glycosides. Ethanolic extract from leaves showed the best antioxidant activity (EC50 = 0.096 mg/ml) in the DPPH assay and the juice (EC50 = 0.022 mg/ml) for the Hydroxyl free radical-scavenging activity. All extracts inhibited more than 98% the hemolysis induced by H2O2. The aqueous extract from leaves showed the highest cytotoxic activity against SW480 and SW620 cells. Conclusions: Findings from this study suggest that P. edulis is a potential source of phytochemical compounds with antioxidant and antiproliferative properties. |
| publishDate |
2013 |
| dc.date.issued.none.fl_str_mv |
2013 |
| dc.date.accessioned.none.fl_str_mv |
2019-07-09T14:44:05Z |
| dc.date.available.none.fl_str_mv |
2019-07-09T14:44:05Z |
| dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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Aguillón J, Maldonado ME, Loango N, Arango SS, Landázuri P. Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis. Perspect. Nutr. Humana.. 2013; 15:13-25. |
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0124-4108 |
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http://hdl.handle.net/10495/11380 |
| dc.identifier.eissn.none.fl_str_mv |
2248-454X |
| identifier_str_mv |
Aguillón J, Maldonado ME, Loango N, Arango SS, Landázuri P. Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis. Perspect. Nutr. Humana.. 2013; 15:13-25. 0124-4108 2248-454X |
| url |
http://hdl.handle.net/10495/11380 |
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spa |
| language |
spa |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Perspect. Nutr. Humana. |
| dc.rights.*.fl_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
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info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) https://creativecommons.org/licenses/by-nc-sa/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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openAccess |
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application/pdf |
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Universidad de Antioquia, Escuela de Nutrición y Dietética |
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Impacto de Componentes Alimentarios en la Salud |
| dc.publisher.place.spa.fl_str_mv |
Medellín, Colombia |
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Universidad de Antioquia |
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http://bibliotecadigital.udea.edu.co/bitstream/10495/11380/1/MaldonadoMaria_2013_AntioxidantAntiproliferativeactivityEthanolic.pdf http://bibliotecadigital.udea.edu.co/bitstream/10495/11380/2/license_url http://bibliotecadigital.udea.edu.co/bitstream/10495/11380/3/license_text http://bibliotecadigital.udea.edu.co/bitstream/10495/11380/4/license_rdf http://bibliotecadigital.udea.edu.co/bitstream/10495/11380/5/license.txt |
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andres.perez@udea.edu.co |
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Aguillón Osma, JohannyMaldonado Celis, María ElenaLoango Chamorro, NelsyArango Varela, Sandra SulayLandázuri, Patricia2019-07-09T14:44:05Z2019-07-09T14:44:05Z2013Aguillón J, Maldonado ME, Loango N, Arango SS, Landázuri P. Antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulis. Perspect. Nutr. Humana.. 2013; 15:13-25.0124-4108http://hdl.handle.net/10495/113802248-454XABSTRACT: Extracts from a variety of fruit trees have been used for therapeutic applications for preventing oxidative stress associated to chronic diseases. Objective: To investigate the antioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits of Passiflora edulis. Materials and methods: A preliminary phytochemical screening was performed; antioxidant activity was evaluated through DPPH assay, the scavenging activity for hydroxyl radical, antihemolytic activity and total phenolic content; cytotoxic and antiproliferative activities were evaluated by MTT and sulforhodamine B assays respectively in colon adenocarcinoma SW480 cells and their metastatic-derived SW620 cells. Results: Phytochemical analyses revealed presence of tannins, flavonoids and cardiotonic glycosides. Ethanolic extract from leaves showed the best antioxidant activity (EC50 = 0.096 mg/ml) in the DPPH assay and the juice (EC50 = 0.022 mg/ml) for the Hydroxyl free radical-scavenging activity. All extracts inhibited more than 98% the hemolysis induced by H2O2. The aqueous extract from leaves showed the highest cytotoxic activity against SW480 and SW620 cells. Conclusions: Findings from this study suggest that P. edulis is a potential source of phytochemical compounds with antioxidant and antiproliferative properties.RESUMEN: Extractos de varios árboles frutales se han utilizado en diferentes aplicaciones terapéuticas para prevenir el estrés oxidativo asociado a enfermedades crónicas. Objetivo: investigar la actividad antioxidante y antiproliferativa de extractos etanólico y acuosos de las hojas y del fruto de Passiflora edulis. Materiales y métodos: se realizó marcha fitoquímica preliminar, la actividad antioxidante se evaluó por DPPH, actividad de remoción de radicales hidroxilo, actividad antihemolítica y contenido fenólico total. En células de adenocarcinoma de colon SW480 y sus derivadas metastásicas SW620 se evaluó la actividad citotóxica y antiproliferativa por el método de MTT y sulforodamina B respectivamente. Resultados: el análisis fitoquímico reveló la presencia de taninos, flavonoides y glicósidos cardiotónicos. El extracto etanólico de las hojas mostró la mayor actividad antioxidante (EC50 = 0,096 mg/ml) por DPPH y el jugo (EC50 = 0,022 mg/ml) para remover el radical hidroxilo. Todos los extractos inhibieron más del 98% la hemólisis inducida por H2O2. El extracto acuoso de las hojas mostró la mayor actividad citotóxica y antiproliferativa contra células SW480 y SW620. Conclusiones: los hallazgos de este estudio sugieren que P. edulis es una fuente potencial de compuestos fitoquímicos con propiedades antioxidante y antiproliferativa.application/pdfspaUniversidad de Antioquia, Escuela de Nutrición y DietéticaImpacto de Componentes Alimentarios en la SaludMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/Passiflora edulisAntioxidantAntiproliferativeCytotoxicAntioxidant and antiproliferative activity of ethanolic and aqueous extracts from leaves and fruits juice Passiflora edulisActividad antioxidante y antiproliferativa de extractos etanólico y acuoso de las hojas y el jugo del fruto de Passiflora edulisPerspect. Nutr. Humana.Perspectivas en Nutrición Humana1325151ORIGINALMaldonadoMaria_2013_AntioxidantAntiproliferativeactivityEthanolic.pdfMaldonadoMaria_2013_AntioxidantAntiproliferativeactivityEthanolic.pdfArtículo de investigaciónapplication/pdf1135758http://bibliotecadigital.udea.edu.co/bitstream/10495/11380/1/MaldonadoMaria_2013_AntioxidantAntiproliferativeactivityEthanolic.pdf2968817633cea985064473617b2ed270MD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://bibliotecadigital.udea.edu.co/bitstream/10495/11380/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/11380/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfLicenciaapplication/rdf+xml; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/11380/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/11380/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5510495/11380oai:bibliotecadigital.udea.edu.co:10495/113802021-10-25 13:38:08.99Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |