Concordance analysis between different methodologies used for identification of oral isolates of Candida species

ABSTRACT: Background: The yeasts species determination is fundamental not only for an accurate diagnosis but also for establishing a suitable patient treatment. We performed a concordance study of five methodologies for the species identification of oral isolates of Candida in Colombia. Methods: Six...

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Autores:
Zuluaga, Alejandra
Arango Bustamante, Karen
Cáceres, Diego
Sánchez Quitian, Zilpa
Velásquez, Verónica
Gómez, Beatriz
Parra Giraldo, Claudia
Maldonado, Natalia
Cano Restrepo, Luz Elena
De Bedout, Catalina
Rivera, Raúl
Tipo de recurso:
Article of investigation
Fecha de publicación:
2018
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/21503
Acceso en línea:
http://hdl.handle.net/10495/21503
Palabra clave:
Mucosa Bucal
Mouth Mucosa
Diagnóstico
Diagnosis
Reacción en Cadena de la Polimerasa
Polymerase Chain Reaction
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Métodos de Análisis
Analytical Methods
Candida
http://aims.fao.org/aos/agrovoc/c_1247
Rights
openAccess
License
http://creativecommons.org/licenses/by-nc-nd/2.5/co/
Description
Summary:ABSTRACT: Background: The yeasts species determination is fundamental not only for an accurate diagnosis but also for establishing a suitable patient treatment. We performed a concordance study of five methodologies for the species identification of oral isolates of Candida in Colombia. Methods: Sixty-seven Candida isolates were tested by; API® 20C-AUX,Vitek®2 Compact, Vitek®MS, Microflex® and a molecular test (panfungal PCR and sequencing). The commercial cost and processing time of the samples was done by graphical analysis. Results: Panfungal PCR differentiated 12 species of Candida, Vitek®MS and Microflex® methods identified 9 species, and API® 20C-AUX and Vitek®2 Compact methods identified 8 species each. Weighted Kappa (wK) showed a high agreement between Panfungal PCR, Vitek®MS, Microflex® and API® 20C-AUX (wK 0.62-0.93). The wK that involved the Vitek®2 Compact method presented moderate or good concordances compared with the other methods (wK 0.56-0.73). Methodologies based on MALDI TOF MS required 4 minutes to generate results and the Microflex® method had the lowest selling price. Conclusion: The methods evaluated showed high concordance in their results, being higher for the molecular methods and the methodologies based on MALDI TOF. The latter are faster and cheaper, presenting as promising alternatives for the routine identification of yeast species of the genus Candida.