Efecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)

ABSTRACT: Chlorpyrifos is a highly toxic insecticide to freshwater organisms and little is known regarding its potential to affect endocrine systems at sublethal concentrations. The induction of vitellogenin (Vtg) is a fairly sensitive marker of the effects of estrogenic compounds in juvenile fish....

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Autores:
Aranzazu Taborda, Diego Alfonso
Rodríguez, Berardo de Jesús
Vieco Durán, Beatriz
Restrepo Betancur, Luis Fernando
Tipo de recurso:
Article of investigation
Fecha de publicación:
2012
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
spa
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/8321
Acceso en línea:
http://hdl.handle.net/10495/8321
Palabra clave:
Biomarcadores
Disruptores endocrinos
Inmunohistoquímica
Inmunohistoquímica organofosforados
Tilapia roja: Oreochromis sp.
Vitelogenina
Biomarkers
Endocrine disruption
Immunohistochemistry organophosphate
Compounds
Vitellogenin
Rights
openAccess
License
Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
id UDEA2_1ae9ce88be0d32f4f533bb532a4c9d25
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/8321
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Efecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
dc.title.alternative.spa.fl_str_mv Effect of Chlorpyrifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) in juvenile of tilapia (Oreochromis spp) males
title Efecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
spellingShingle Efecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
Biomarcadores
Disruptores endocrinos
Inmunohistoquímica
Inmunohistoquímica organofosforados
Tilapia roja: Oreochromis sp.
Vitelogenina
Biomarkers
Endocrine disruption
Immunohistochemistry organophosphate
Compounds
Vitellogenin
title_short Efecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
title_full Efecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
title_fullStr Efecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
title_full_unstemmed Efecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
title_sort Efecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
dc.creator.fl_str_mv Aranzazu Taborda, Diego Alfonso
Rodríguez, Berardo de Jesús
Vieco Durán, Beatriz
Restrepo Betancur, Luis Fernando
dc.contributor.author.none.fl_str_mv Aranzazu Taborda, Diego Alfonso
Rodríguez, Berardo de Jesús
Vieco Durán, Beatriz
Restrepo Betancur, Luis Fernando
dc.subject.none.fl_str_mv Biomarcadores
Disruptores endocrinos
Inmunohistoquímica
Inmunohistoquímica organofosforados
Tilapia roja: Oreochromis sp.
Vitelogenina
Biomarkers
Endocrine disruption
Immunohistochemistry organophosphate
Compounds
Vitellogenin
topic Biomarcadores
Disruptores endocrinos
Inmunohistoquímica
Inmunohistoquímica organofosforados
Tilapia roja: Oreochromis sp.
Vitelogenina
Biomarkers
Endocrine disruption
Immunohistochemistry organophosphate
Compounds
Vitellogenin
description ABSTRACT: Chlorpyrifos is a highly toxic insecticide to freshwater organisms and little is known regarding its potential to affect endocrine systems at sublethal concentrations. The induction of vitellogenin (Vtg) is a fairly sensitive marker of the effects of estrogenic compounds in juvenile fish. Objective: to evaluate histological changes and Vtg production in juvenile male tilapia (Oreochromis spp) exposed to sublethal concentrations of the insecticide Lorsban® EC (active ingredient chlorpyriphos). Methods: juvenile tilapia were exposed to 4, 8, and 12 µg/L of chlorpyrifos for 28 days in a semistatic system, with tanks receiving a 50% (v/v) daily water change to maintain nominal concentrations of the insecticide throughout the experiment. Subgroups of 3 animals from each concentration batch were euthanized on days 7, 14, 21 and 28 days, and samples of liver, gonads, gills, kidney and brain tissues were taken for routine histopathology examination. Liver and gonads were also processed by immunohistochemistry using monoclonal anti-killifish vitellogenin Vtg ND - 5F8 to detect Vtg. Results: we found significant statistical difference (p < 0.05) for some injuries to the brain (degeneration and gliosis of the optic tectum), kidneys (vacuolar nephrosis and tubular hyaline granules), and liver (karyomegaly, binucleation, and hyaline granules in hepatocytes). Similarly we verified the induction of vitellogenin synthesis in liver and gonads, finding significant difference (p < 0.05) in the expression of this protein between the control group and 4 mg / L with respect to treatment of 8 and 12 mg / L. Conclusion: the results obtained on the induction of vitellogenin in males suggest a general effect of blocking concentrations of Chlorpyrifos for the possible induction of this protein. The mechanisms are not known at this time.
publishDate 2012
dc.date.issued.none.fl_str_mv 2012
dc.date.accessioned.none.fl_str_mv 2017-09-26T13:15:32Z
dc.date.available.none.fl_str_mv 2017-09-26T13:15:32Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.local.spa.fl_str_mv Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv Aranzazu DA, Rodríguez B de J, Vieco B, Restrepo LF. Efecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp). Rev. Colomb. Cienc. Pecu. 2012;25(2):276-291.
dc.identifier.issn.none.fl_str_mv 0120-0690
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/8321
dc.identifier.eissn.none.fl_str_mv 2256-2958
identifier_str_mv Aranzazu DA, Rodríguez B de J, Vieco B, Restrepo LF. Efecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp). Rev. Colomb. Cienc. Pecu. 2012;25(2):276-291.
0120-0690
2256-2958
url http://hdl.handle.net/10495/8321
dc.language.iso.spa.fl_str_mv spa
language spa
dc.relation.ispartofjournalabbrev.spa.fl_str_mv Rev. Colomb. Cienc. Pecu.
dc.rights.*.fl_str_mv Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
https://creativecommons.org/licenses/by-nc-sa/2.5/co/
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eu_rights_str_mv openAccess
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv Universidad de Antioquia, Facultad de Ciencias Agrarias
dc.publisher.group.spa.fl_str_mv CENTAURO
dc.publisher.place.spa.fl_str_mv Medellín, Colombia
institution Universidad de Antioquia
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spelling Aranzazu Taborda, Diego AlfonsoRodríguez, Berardo de JesúsVieco Durán, BeatrizRestrepo Betancur, Luis Fernando2017-09-26T13:15:32Z2017-09-26T13:15:32Z2012Aranzazu DA, Rodríguez B de J, Vieco B, Restrepo LF. Efecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp). Rev. Colomb. Cienc. Pecu. 2012;25(2):276-291.0120-0690http://hdl.handle.net/10495/83212256-2958ABSTRACT: Chlorpyrifos is a highly toxic insecticide to freshwater organisms and little is known regarding its potential to affect endocrine systems at sublethal concentrations. The induction of vitellogenin (Vtg) is a fairly sensitive marker of the effects of estrogenic compounds in juvenile fish. Objective: to evaluate histological changes and Vtg production in juvenile male tilapia (Oreochromis spp) exposed to sublethal concentrations of the insecticide Lorsban® EC (active ingredient chlorpyriphos). Methods: juvenile tilapia were exposed to 4, 8, and 12 µg/L of chlorpyrifos for 28 days in a semistatic system, with tanks receiving a 50% (v/v) daily water change to maintain nominal concentrations of the insecticide throughout the experiment. Subgroups of 3 animals from each concentration batch were euthanized on days 7, 14, 21 and 28 days, and samples of liver, gonads, gills, kidney and brain tissues were taken for routine histopathology examination. Liver and gonads were also processed by immunohistochemistry using monoclonal anti-killifish vitellogenin Vtg ND - 5F8 to detect Vtg. Results: we found significant statistical difference (p < 0.05) for some injuries to the brain (degeneration and gliosis of the optic tectum), kidneys (vacuolar nephrosis and tubular hyaline granules), and liver (karyomegaly, binucleation, and hyaline granules in hepatocytes). Similarly we verified the induction of vitellogenin synthesis in liver and gonads, finding significant difference (p < 0.05) in the expression of this protein between the control group and 4 mg / L with respect to treatment of 8 and 12 mg / L. Conclusion: the results obtained on the induction of vitellogenin in males suggest a general effect of blocking concentrations of Chlorpyrifos for the possible induction of this protein. The mechanisms are not known at this time.RESUMEN: Hasta donde se conoce, no hay estudios en tilapias juveniles (Oreochromis spp), que valoren el potencial de disrupción endocrina del Clorpirifos por análisis histológico e inmunohistoquímico de la inducción de la Vitelogenina (Vtg) hepática. Objetivo: determinar los efectos de la exposición subaguda al Clorpirifos en órganos blanco de disrupción en peces juveniles machos de tilapia. Métodos: el experimento de dosis subletal, se realizó en un sistema semiestático, con recambio diario del 50% del volumen de agua manteniendo la concentración nominal en cada grupo experimental mediante la adición de la mitad de la dosis hasta el día 28. Las concentraciones de Clorpirifos para la exposición fueron 4, 8, y 12 μg/L. Con cada concentración se trataron 12 juveniles, con tres replicas para cada concentración. Los 12 peces del grupo control no recibieron tratamiento. Se realizó el estudio anatomopatológico de tres animales por grupo, por cada semana los días 7, 14, 21 y 28 de estudio. Se efectuó la toma de muestras para estudio histopatológico de hígado, gónadas, branquias, riñón y encéfalo y se procesaron por histopatología de rutina. Las muestras de hígado y gónada también se procesaron por inmunohistoquímica. Resultados: el análisis MANOVA encontró diferencia significativa (p < 0.05) para lesiones en encéfalo (degeneración tectum óptico), riñón (gránulos hialinos) e hígado (cariomegalia), constituyéndose en órganos de impacto de los efectos del Clorpirifos a bajas dosis. Se verificó la inducción de Vtg en hígado y gónada de los animales expuestos, encontrando diferencia estadística significativa (p < 0.05) en la expresión de esta proteína entre el grupo control y de 4 μg/L con relación a los grupos de 8 y 12 μg/L. Conclusión: los resultados obtenidos sobre la inducción de Vtg en machos sugieren un efecto antogonista del Clorpirifos sobre los Receptores Estrogénicos (REs), con una posible disminución en la síntesis de ésta proteína. Los mecanismos no se conocen en el momento.COL0001262application/pdfspaUniversidad de Antioquia, Facultad de Ciencias AgrariasCENTAUROMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/BiomarcadoresDisruptores endocrinosInmunohistoquímicaInmunohistoquímica organofosforadosTilapia roja: Oreochromis sp.VitelogeninaBiomarkersEndocrine disruptionImmunohistochemistry organophosphateCompoundsVitellogeninEfecto del Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)Effect of Chlorpyrifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) in juvenile of tilapia (Oreochromis spp) malesRev. Colomb. Cienc. Pecu.Revista Colombiana de Ciencias Pecuarias276291252LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/8321/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://bibliotecadigital.udea.edu.co/bitstream/10495/8321/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/8321/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/8321/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54ORIGINALAranzazuDiego_2012_EfectoClorpirifosMachosTilapa.pdfAranzazuDiego_2012_EfectoClorpirifosMachosTilapa.pdfArtículo de investigaciónapplication/pdf1402023http://bibliotecadigital.udea.edu.co/bitstream/10495/8321/1/AranzazuDiego_2012_EfectoClorpirifosMachosTilapa.pdf4f1a2e0cb862835b29d3f143e41b9863MD5110495/8321oai:bibliotecadigital.udea.edu.co:10495/83212021-05-21 20:29:30.515Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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