Application of process system engineering tools to the fed-batch production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from a vinasses–molasses Mixture

ABSTRACT : Fed-batch production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using vinasses–molasses mixture is carried out in this work by implementing different process systems engineering tools. Two fed-batch strategies are tested experimentally at 5 L scale, considering only offline...

Full description

Autores:
García Echeverry, Cesar Augusto
Alcaraz Zapata, Wilman
Acosta Cárdenas, Alejandro
Ochoa Cáceres, Silvia Mercedes
Tipo de recurso:
Article of investigation
Fecha de publicación:
2019
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/31118
Acceso en línea:
https://hdl.handle.net/10495/31118
Palabra clave:
Técnicas de Cultivo Celular por Lotes
Batch Cell Culture Techniques
Polihidroxialcanoatos
Polyhydroxyalkanoates
Melaza
Molasses
Modelos Biológicos
Models, Biological
Vinazas
Vinasse
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
Description
Summary:ABSTRACT : Fed-batch production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using vinasses–molasses mixture is carried out in this work by implementing different process systems engineering tools. Two fed-batch strategies are tested experimentally at 5 L scale, considering only offline information: (1) offline optimizing control and (2) exponential feeding. Application of these strategies showed that different feeding profiles result in different dynamic behaviour, influencing both, yield and biopolymer properties. As offline-based feeding strategies do not consider information of the culture status, they cannot deal with uncertainties. Therefore, a closed loop control strategy was implemented, which uses biomass and substrate information predicted online by soft-sensors. Results demonstrated the technical feasibility to produce biopolymer using a 75/25%vol. vinasses–molasses mixture. Successful implementation of the soft-sensor-based control strategy was evidenced at pilot plant scale, where sugar concentration was kept almost constant for 14 h, while obtaining the desired copolymer. Thus, proposed control strategy could be of interest at industrial-scale.