Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch
ABSTRACT : Bacillus cereus is a food contaminant and a known human pathogen that can cause emetic and diarrheal syndromes. In this study we evaluated the presence of toxigenic B. cereus by multiplex PCR directly in dietary complement for children and cassava starch samples collected on Medellin, Col...
- Autores:
-
Sánchez Chica, Jennifer
Correa Ochoa, Margarita María
Aceves Díez, Ángel Emilio
Castañeda Sandoval, Laura Margarita
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2014
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/26459
- Acceso en línea:
- http://hdl.handle.net/10495/26459
https://revistas.unal.edu.co/index.php/rcolquim/article/view/53425
- Palabra clave:
- Bacillus cereus
Enterotoxinas
Enterotoxins
Reacción en Cadena de la Polimerasa Multiplex
Multiplex Polymerase Chain Reaction
Alimentos en Conserva
Food, Preserved
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch |
| dc.title.alternative.spa.fl_str_mv |
Detección directa de Bacillus cereus toxigénicos en complementos dietarios para niños y en almidón de yuca Detecção direta de Bacillus cereus toxigênicos em suplementos alimentares para crianças e amido de mandioca |
| title |
Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch |
| spellingShingle |
Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch Bacillus cereus Enterotoxinas Enterotoxins Reacción en Cadena de la Polimerasa Multiplex Multiplex Polymerase Chain Reaction Alimentos en Conserva Food, Preserved |
| title_short |
Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch |
| title_full |
Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch |
| title_fullStr |
Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch |
| title_full_unstemmed |
Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch |
| title_sort |
Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch |
| dc.creator.fl_str_mv |
Sánchez Chica, Jennifer Correa Ochoa, Margarita María Aceves Díez, Ángel Emilio Castañeda Sandoval, Laura Margarita |
| dc.contributor.author.none.fl_str_mv |
Sánchez Chica, Jennifer Correa Ochoa, Margarita María Aceves Díez, Ángel Emilio Castañeda Sandoval, Laura Margarita |
| dc.subject.decs.none.fl_str_mv |
Bacillus cereus Enterotoxinas Enterotoxins Reacción en Cadena de la Polimerasa Multiplex Multiplex Polymerase Chain Reaction Alimentos en Conserva Food, Preserved |
| topic |
Bacillus cereus Enterotoxinas Enterotoxins Reacción en Cadena de la Polimerasa Multiplex Multiplex Polymerase Chain Reaction Alimentos en Conserva Food, Preserved |
| description |
ABSTRACT : Bacillus cereus is a food contaminant and a known human pathogen that can cause emetic and diarrheal syndromes. In this study we evaluated the presence of toxigenic B. cereus by multiplex PCR directly in dietary complement for children and cassava starch samples collected on Medellin, Colombia. Of 75 dietary complement for children samples evaluated, 70.7% were contaminated with toxigenic B. cereus and four different toxigenic consortia were detected: I: nheA, hblC, cytK (9.8%), II: nheA, hblC (2%), III: hblC, cytK (41.2%), IV: hblC (47%). Of 75 cassava starch samples, 44% were contaminated with toxigenic B. cereus and four different toxigenic consortia were determined: I: nheA, hblC, cytK (48.5%), II: nheA, hblC, cytK, cesB (3%), III: hblC, cytK (30.3%), IV: hblC (18.2%). In general, in dietary complement for children only enterotoxigenic consortia were detected while in cassava starch the enterotoxigenic consortia predominated over the emetic. Multiplex PCR was useful to detect toxigenic B. cereus contamination allowing direct and simultaneous detection of all toxin genes in foods. This study is the first in Colombia to evaluate toxigenic B. cereus, providing information of importance for microbiological risk evaluation in dried foods. |
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2014 |
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2014 |
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2022-03-08T18:23:00Z |
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2022-03-08T18:23:00Z |
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Artículo de investigación |
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Sánchez JA, Correa MM, Aceves Dies Ángel E, Castañeda Sandoval LM. Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch. Rev. Colomb. Quim. [Internet]. 1 de mayo de 2014 [citado 8 de marzo de 2022];43(2):5-9. Disponible en: https://revistas.unal.edu.co/index.php/rcolquim/article/view/53425 |
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0120-2804 |
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http://hdl.handle.net/10495/26459 |
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10.15446/rev.colomb.quim.v43n2.53117 |
| dc.identifier.eissn.none.fl_str_mv |
2357-3791 |
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https://revistas.unal.edu.co/index.php/rcolquim/article/view/53425 |
| identifier_str_mv |
Sánchez JA, Correa MM, Aceves Dies Ángel E, Castañeda Sandoval LM. Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch. Rev. Colomb. Quim. [Internet]. 1 de mayo de 2014 [citado 8 de marzo de 2022];43(2):5-9. Disponible en: https://revistas.unal.edu.co/index.php/rcolquim/article/view/53425 0120-2804 10.15446/rev.colomb.quim.v43n2.53117 2357-3791 |
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eng |
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Rev. Colomb. Quím. |
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Universidad Nacional de Colombia, Facultad de Ciencias, Departamento de Química |
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BIOMICRO |
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Bogotá, Colombia |
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Sánchez Chica, JenniferCorrea Ochoa, Margarita MaríaAceves Díez, Ángel EmilioCastañeda Sandoval, Laura Margarita2022-03-08T18:23:00Z2022-03-08T18:23:00Z2014Sánchez JA, Correa MM, Aceves Dies Ángel E, Castañeda Sandoval LM. Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starch. Rev. Colomb. Quim. [Internet]. 1 de mayo de 2014 [citado 8 de marzo de 2022];43(2):5-9. Disponible en: https://revistas.unal.edu.co/index.php/rcolquim/article/view/534250120-2804http://hdl.handle.net/10495/2645910.15446/rev.colomb.quim.v43n2.531172357-3791https://revistas.unal.edu.co/index.php/rcolquim/article/view/53425ABSTRACT : Bacillus cereus is a food contaminant and a known human pathogen that can cause emetic and diarrheal syndromes. In this study we evaluated the presence of toxigenic B. cereus by multiplex PCR directly in dietary complement for children and cassava starch samples collected on Medellin, Colombia. Of 75 dietary complement for children samples evaluated, 70.7% were contaminated with toxigenic B. cereus and four different toxigenic consortia were detected: I: nheA, hblC, cytK (9.8%), II: nheA, hblC (2%), III: hblC, cytK (41.2%), IV: hblC (47%). Of 75 cassava starch samples, 44% were contaminated with toxigenic B. cereus and four different toxigenic consortia were determined: I: nheA, hblC, cytK (48.5%), II: nheA, hblC, cytK, cesB (3%), III: hblC, cytK (30.3%), IV: hblC (18.2%). In general, in dietary complement for children only enterotoxigenic consortia were detected while in cassava starch the enterotoxigenic consortia predominated over the emetic. Multiplex PCR was useful to detect toxigenic B. cereus contamination allowing direct and simultaneous detection of all toxin genes in foods. This study is the first in Colombia to evaluate toxigenic B. cereus, providing information of importance for microbiological risk evaluation in dried foods.RESUMEN : Bacillus cereus es un contaminante de alimentos conocido por ser patogénico para los humanos, causando síndromes de vómito y diarrea. En este estudio se evaluó la presencia de B. cereus toxigénicos utilizando PCR múltiple directamente en complementos dietarios para niños y en almidón de yuca colectados en Medellín, Colombia. De 75 muestras de complemento dietario para niños, 70,7% estuvieron contaminadas con B. cereus toxigénicos y se detectaron cuatro diferentes consorcios toxigénicos: I: nheA, hblC, cytK (9,8%), II: nheA, hblC (2%), III: hblC, cytK (41.2%), IV: hblC (47%). De 75 muestras de almidón de yuca, 44% estuvieron contaminadas con B. cereus toxigénicos y se determinaron cuatro diferentes consorcios toxigénicos: I: nheA, hblC, cytK (48.5%), II: nheA, hblC, cytK, cesB (3%), III: hblC, cytK (30,3%), IV: hblC (18.2%). En general, en los complementos dietarios para niños sólo se detectaron consorcios enterotoxigénicos, mientras que en el almidón los consorcios enterotoxigénicos predominaron sobre el emético. La PCR múltiple fue de utilidad para detectar contaminación con B. cereus toxigénicos permitiendo la detección directa y simultánea de todos los genes tóxicos en los alimentos. Este estudio es el primero en Colombia en evaluar B. cereus toxigénicos y proporciona información importante para la evaluación de riesgos microbiológicos en los alimentos pulverizados.RESUMO : Bacillus cereus é um contaminante de alimentos e é conhecido por ser patogénico nos seres humanos ocasionando síndromes de vômitos e diarreia. Neste estudo foi avaliada a presença de B. cereus toxigênicos por PCR multiplex diretamente em complementos da dieta para crianças e amido de mandioca, em amostras coletadas em Medellín, na Colômbia. De 75 amostras dos complementos da dieta para crianças, 70,7% estiveram contaminadas com B. cereus toxigênicos e foram detectados quatro diferentes consórcios: I: nheA, hblC, cytK (9,8%), II: nheA, hblC (2%), III: hblC, cytK (41,2%), IV: hblC (47%). De 75 amostras de amido de mandioca, 44% estiveram contaminadas com B. cereus toxigênicos e quatro consórcios diferentes foram determinados: I: nheA, hblC, cytK (48,5%), II: nheA, hblC, cytK, cesB (3%), III: hblC, cytK (30,3%), IV: hblC (18,2%). Em geral, nos complementos da dieta para crianças foram detectados apenas consórcios enterotoxigênicos, não obstante no amido os consórcios enterotoxigênicos predominaram sobre o emético. A PCR multiplex foi útil para detectar contaminação com B. cereus toxigênico permitindo a detecção direta e simultânea de todos os genes tóxicos em alimentos. Este estudo é o primeiro na Colômbia em avaliar B. cereus toxigênico e providencia informação importante para a avaliação de riscos microbiológicos em alimentos pulverizados.COL01118635application/pdfengUniversidad Nacional de Colombia, Facultad de Ciencias, Departamento de QuímicaBIOMICROBogotá, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Direct detection of toxigenic Bacillus cereus in dietary complement for children and cassava starchDetección directa de Bacillus cereus toxigénicos en complementos dietarios para niños y en almidón de yucaDetecção direta de Bacillus cereus toxigênicos em suplementos alimentares para crianças e amido de mandiocaBacillus cereusEnterotoxinasEnterotoxinsReacción en Cadena de la Polimerasa MultiplexMultiplex Polymerase Chain ReactionAlimentos en ConservaFood, PreservedRev. Colomb. Quím.Revista Colombiana de Química59432CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927http://bibliotecadigital.udea.edu.co/bitstream/10495/26459/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/26459/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINALSánchezJennifer_2014_BacillusDietaryChildren.pdfSánchezJennifer_2014_BacillusDietaryChildren.pdfArtículo de investigaciónapplication/pdf161086http://bibliotecadigital.udea.edu.co/bitstream/10495/26459/1/S%c3%a1nchezJennifer_2014_BacillusDietaryChildren.pdfaa8ccf146d2bdaf5d6088a324f0df302MD5110495/26459oai:bibliotecadigital.udea.edu.co:10495/264592022-04-22 10:23:22.045Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |