Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines
ABSTRACT: Marine sponges are considered an important source of substances with pharmacological potential. They play a key role in the intensive investigation of promising new compounds to treat cancer and other diseases. Objective: To evaluate in CHOK1 and Jurkat cell lines, the cytotoxic, genotoxic...
- Autores:
-
De la Ossa, Mauricio
López Ortiz, Juan Bautista
Márquez Fernández, Diana Margarita
Martínez Martínez, Alejandro
Márquez Fernández, María Elena
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2016
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/13119
- Acceso en línea:
- http://hdl.handle.net/10495/13119
http://www.revfarmacia.sld.cu/index.php/far/rt/printerFriendly/63/67
- Palabra clave:
- Actividad biológica
Efectos citotóxicos
Lotrochota Birotulata
Células de mamíferos
Jurkat
CHOK1
- Rights
- openAccess
- License
- Atribución-NoComercial-SinDerivadas 2.5 Colombia
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| dc.title.spa.fl_str_mv |
Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines |
| dc.title.alternative.spa.fl_str_mv |
Actividad biológica de fracciones de la esponja marina Iotrochota birotulata en células de mamíferos |
| title |
Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines |
| spellingShingle |
Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines Actividad biológica Efectos citotóxicos Lotrochota Birotulata Células de mamíferos Jurkat CHOK1 |
| title_short |
Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines |
| title_full |
Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines |
| title_fullStr |
Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines |
| title_full_unstemmed |
Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines |
| title_sort |
Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines |
| dc.creator.fl_str_mv |
De la Ossa, Mauricio López Ortiz, Juan Bautista Márquez Fernández, Diana Margarita Martínez Martínez, Alejandro Márquez Fernández, María Elena |
| dc.contributor.author.none.fl_str_mv |
De la Ossa, Mauricio López Ortiz, Juan Bautista Márquez Fernández, Diana Margarita Martínez Martínez, Alejandro Márquez Fernández, María Elena |
| dc.subject.none.fl_str_mv |
Actividad biológica Efectos citotóxicos Lotrochota Birotulata Células de mamíferos Jurkat CHOK1 |
| topic |
Actividad biológica Efectos citotóxicos Lotrochota Birotulata Células de mamíferos Jurkat CHOK1 |
| description |
ABSTRACT: Marine sponges are considered an important source of substances with pharmacological potential. They play a key role in the intensive investigation of promising new compounds to treat cancer and other diseases. Objective: To evaluate in CHOK1 and Jurkat cell lines, the cytotoxic, genotoxic and antiproliferative effects of two fractions of I. birotulata sponge from Colombian Caribbean. Methods: The cell viability (cytotoxic effect) was determined by Trypan blue exclusion and MTT assays. Genotoxicity was assessed by single cell gel electrophoresis, the antiproliferative effect was monitored with clonogenic test, sister chromatid exchange proliferative kinetic, and accumulation function. Data was analyzed with lineal regression, oneway ANOVA, and Bonferroni tests. Results: Both cytotoxic assays showed a similar dose dependent effect for the CHOK1 and Jurkat cell lines treated with both fractions (F5 y F6) of I. birotulata. They also revealed an effect on the cell membrane and mitochondrial activity of both cell lines. Fraction F5 exhibited a greater genotoxic effect on both cell lines, which is consistent with the antiproliferation results obtained by the clonogenic assay. These results are also consistent with the inhibitory effect on the cell cycle, which was evaluated with SCE, proliferative kinetic, and the accumulation function tests. Consequently, the results showed differential sensitivity to the treatment of the Jurkat cells compared to the CHOK1 cell line. Conclusions: Together, the results show a differential effect of the two assessed fractions on cell membrane integrity, mitochondrial activity, and antiproliferative effect on both mammalian cell lines. |
| publishDate |
2016 |
| dc.date.issued.none.fl_str_mv |
2016 |
| dc.date.accessioned.none.fl_str_mv |
2020-01-14T02:58:37Z |
| dc.date.available.none.fl_str_mv |
2020-01-14T02:58:37Z |
| dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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De la Ossa, M., López Ortiz, J. B., Márquez Fernández, D. M., Martínez Martínez, A., & Márquez Fernández, M. E. (2016). Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines. Revista Cubana de Farmacia, 50(4), 1-16. |
| dc.identifier.issn.none.fl_str_mv |
0034-7515 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/13119 |
| dc.identifier.doi.none.fl_str_mv |
http://www.revfarmacia.sld.cu/index.php/far/rt/printerFriendly/63/67 |
| dc.identifier.eissn.none.fl_str_mv |
1561-2988 |
| identifier_str_mv |
De la Ossa, M., López Ortiz, J. B., Márquez Fernández, D. M., Martínez Martínez, A., & Márquez Fernández, M. E. (2016). Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines. Revista Cubana de Farmacia, 50(4), 1-16. 0034-7515 1561-2988 |
| url |
http://hdl.handle.net/10495/13119 http://www.revfarmacia.sld.cu/index.php/far/rt/printerFriendly/63/67 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.rights.*.fl_str_mv |
Atribución-NoComercial-SinDerivadas 2.5 Colombia |
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info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
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Atribución-NoComercial-SinDerivadas 2.5 Colombia http://creativecommons.org/licenses/by-nc-nd/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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application/pdf |
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Editorial Ciencias Médicas |
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Productos Naturales Marinos |
| institution |
Universidad de Antioquia |
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De la Ossa, MauricioLópez Ortiz, Juan BautistaMárquez Fernández, Diana MargaritaMartínez Martínez, AlejandroMárquez Fernández, María Elena2020-01-14T02:58:37Z2020-01-14T02:58:37Z2016De la Ossa, M., López Ortiz, J. B., Márquez Fernández, D. M., Martínez Martínez, A., & Márquez Fernández, M. E. (2016). Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell lines. Revista Cubana de Farmacia, 50(4), 1-16.0034-7515http://hdl.handle.net/10495/13119http://www.revfarmacia.sld.cu/index.php/far/rt/printerFriendly/63/671561-2988ABSTRACT: Marine sponges are considered an important source of substances with pharmacological potential. They play a key role in the intensive investigation of promising new compounds to treat cancer and other diseases. Objective: To evaluate in CHOK1 and Jurkat cell lines, the cytotoxic, genotoxic and antiproliferative effects of two fractions of I. birotulata sponge from Colombian Caribbean. Methods: The cell viability (cytotoxic effect) was determined by Trypan blue exclusion and MTT assays. Genotoxicity was assessed by single cell gel electrophoresis, the antiproliferative effect was monitored with clonogenic test, sister chromatid exchange proliferative kinetic, and accumulation function. Data was analyzed with lineal regression, oneway ANOVA, and Bonferroni tests. Results: Both cytotoxic assays showed a similar dose dependent effect for the CHOK1 and Jurkat cell lines treated with both fractions (F5 y F6) of I. birotulata. They also revealed an effect on the cell membrane and mitochondrial activity of both cell lines. Fraction F5 exhibited a greater genotoxic effect on both cell lines, which is consistent with the antiproliferation results obtained by the clonogenic assay. These results are also consistent with the inhibitory effect on the cell cycle, which was evaluated with SCE, proliferative kinetic, and the accumulation function tests. Consequently, the results showed differential sensitivity to the treatment of the Jurkat cells compared to the CHOK1 cell line. Conclusions: Together, the results show a differential effect of the two assessed fractions on cell membrane integrity, mitochondrial activity, and antiproliferative effect on both mammalian cell lines.RESUMEN: Las esponjas marinas son una fuente importante de compuestos con propiedades farmacológicas potenciales. Desempeñan una función esencial en la intensa en la búsqueda de nuevas y prometedores compuestos que brinden tratamiento para el cáncer y otras enfermedades. Objetivo: Evaluar los efectos citotóxico, genotóxico y antiproliferativo en las líneas celulares CHOK1 y Jurkat de dos fracciones de la esponja Iotrochota birotulata del Caribe colombiano. Métodos: La viabilidad celular (efecto citotóxico) se determinó mediante ensayos de azul de Tripano y MTT. La genotoxicidad se evaluó mediante la electroforesis en gel de células individuales y el efecto antiproliferativo se monitoreó con pruebas clonogénica, cinética proliferativa por intercambio de cromátides hermanas y la función de acumulación. Los datos se analizaron mediante regresión lineal, ANOVA de una vía, y la prueba de Bonferroni. Resultados: Ambas pruebas citotóxicas mostraron un efecto similar dependiente de la dosis en las líneas celulares CHOK1 y Jurkat tratadas con dos fracciones (F5 y F6) de I. birotulata. Las pruebas citotóxicas también revelaron efectos en la membrana celular y en la actividad mitocondrial en ambas líneas celulares. La fracción F5 mostró mayor efecto genotóxico en las dos líneas celulares, consistente con los resultados de antiproliferación del ensayo clonogénico y con el efecto inhibitorio en el ciclo celular evaluado por las pruebas de intercambio de cromátides hermanas, cinética proliferativa y función de acumulación. Por consiguiente, los resultados revelaron mayor sensibilidad diferencial al tratamiento en las células Jurkat comparada con la línea celular CHOK1. Conclusiones: Los datos obtenidos demuestran el efecto diferencial de las fracciones F5 y F6, en la integridad de la membrana celular, la actividad mitocondrial y el efecto antiproliferativo en las dos líneas celulares de mamíferos evaluadas.application/pdfengEditorial Ciencias MédicasProductos Naturales Marinosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-SinDerivadas 2.5 Colombiainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Actividad biológicaEfectos citotóxicosLotrochota BirotulataCélulas de mamíferosJurkatCHOK1Biological activity of fractions from the marine sponge Iotrochota birotulata IN mammalian cell linesActividad biológica de fracciones de la esponja marina Iotrochota birotulata en células de mamíferosRevista Cubana de Farmacia116504CC-LICENSElicense_urllicense_urlArtículo de investigacióntext/plain; charset=utf-849http://bibliotecadigital.udea.edu.co/bitstream/10495/13119/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/13119/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/13119/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/13119/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55ORIGINALMartinezAlejandro_2016_BiologicalActivityFractions.pdfMartinezAlejandro_2016_BiologicalActivityFractions.pdfArtículo de revistaapplication/pdf525525http://bibliotecadigital.udea.edu.co/bitstream/10495/13119/1/MartinezAlejandro_2016_BiologicalActivityFractions.pdf90e4925aa882974b80ec78e5bbdefc66MD5110495/13119oai:bibliotecadigital.udea.edu.co:10495/131192021-05-16 11:49:30.562Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |