Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells
ABSTRACT: AIM: Procyanidins (Pcy) are oligomers of catechins and epicatechins. In order to get more insight into the apoptotic effects of Pcy, we aimed to compare the death signaling pathways triggered by Pcy in TRAIL-sensitive human colon cancer cells (SW480) and in their derived TRAIL-resistant me...
- Autores:
-
Maldonado Celis, María Elena
Bousserouel, Souad
Gossé, Francine
Minker, Carole
Lobstein, Annelise
Raul, Francis
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2009
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/21982
- Acceso en línea:
- http://hdl.handle.net/10495/21982
- Palabra clave:
- Apoptosis
Colorectal Neoplasms
Neoplasias Colorrectales
Flavonoids
Flavonoides
Trail Making Test
Prueba de Secuencia Alfanumérica
Mitochondria
Mitocondrias
- Rights
- openAccess
- License
- https://creativecommons.org/licenses/by-nc-nd/2.5/co/
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oai:bibliotecadigital.udea.edu.co:10495/21982 |
network_acronym_str |
UDEA2 |
network_name_str |
Repositorio UdeA |
repository_id_str |
|
dc.title.spa.fl_str_mv |
Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells |
title |
Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells |
spellingShingle |
Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells Apoptosis Colorectal Neoplasms Neoplasias Colorrectales Flavonoids Flavonoides Trail Making Test Prueba de Secuencia Alfanumérica Mitochondria Mitocondrias |
title_short |
Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells |
title_full |
Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells |
title_fullStr |
Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells |
title_full_unstemmed |
Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells |
title_sort |
Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells |
dc.creator.fl_str_mv |
Maldonado Celis, María Elena Bousserouel, Souad Gossé, Francine Minker, Carole Lobstein, Annelise Raul, Francis |
dc.contributor.author.none.fl_str_mv |
Maldonado Celis, María Elena Bousserouel, Souad Gossé, Francine Minker, Carole Lobstein, Annelise Raul, Francis |
dc.subject.decs.none.fl_str_mv |
Apoptosis Colorectal Neoplasms Neoplasias Colorrectales Flavonoids Flavonoides Trail Making Test Prueba de Secuencia Alfanumérica Mitochondria Mitocondrias |
topic |
Apoptosis Colorectal Neoplasms Neoplasias Colorrectales Flavonoids Flavonoides Trail Making Test Prueba de Secuencia Alfanumérica Mitochondria Mitocondrias |
description |
ABSTRACT: AIM: Procyanidins (Pcy) are oligomers of catechins and epicatechins. In order to get more insight into the apoptotic effects of Pcy, we aimed to compare the death signaling pathways triggered by Pcy in TRAIL-sensitive human colon cancer cells (SW480) and in their derived TRAIL-resistant metastatic cells (SW620). METHODS: Cell death, DR4/DR5, Bcl-2/Bax proteins, mitochondrial membrane potential, and reactive oxygen species (ROS) production were analyzed by flow cytometry. Bid and cytochrome c were studied by immunoblotting and ELISA. RESULTS: Pcy (80 µg/ml) combined with exogenous TRAIL (30 ng/ml) enhanced the apoptotic effects of TRAIL in SW480 but also in SW620 cells, indicating that Pcy sensitized SW620 cells to TRAIL treatment. Pcy up-regulated the expression of death receptors DR4/DR5 of TRAIL. Activation of caspase-8 and -3 was observed in both cell lines but caspase-9 was activated only in SW620 cells. Pcy-treated SW620 cells exhibited a decrease in Bid protein level, in Bcl-2/Bax ratio, and enhanced intracellular ROS production. These events were associated with the decrease of mitochondrial membrane potential and the release of cytochrome c into the cytosol. CONCLUSION: Our data suggest that Pcy triggers apoptosis via caspase-8 and may enhance TRAIL-induced apoptosis by activation of the extrinsic pathway in both cell lines. In the TRAIL-resistant SW620 cells, the apoptotic effects of Pcy were complex and may involve a cross-talk between the extrinsic and the mitochondrial apoptotic pathways and a Pcy-triggered intracellular ROS production favoring mitochondrial disruption. These data highlight the chemopreventive potentials of apple Pcy in colorectal cancer treatments. |
publishDate |
2009 |
dc.date.issued.none.fl_str_mv |
2009 |
dc.date.accessioned.none.fl_str_mv |
2021-08-30T16:48:52Z |
dc.date.available.none.fl_str_mv |
2021-08-30T16:48:52Z |
dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.coarversion.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
dc.type.hasversion.spa.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_2df8fbb1 |
dc.type.redcol.spa.fl_str_mv |
https://purl.org/redcol/resource_type/ART |
dc.type.local.spa.fl_str_mv |
Artículo de investigación |
format |
http://purl.org/coar/resource_type/c_2df8fbb1 |
status_str |
publishedVersion |
dc.identifier.issn.none.fl_str_mv |
1816-0735 |
dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/21982 |
dc.identifier.eissn.none.fl_str_mv |
1817-4256 |
identifier_str_mv |
1816-0735 1817-4256 |
url |
http://hdl.handle.net/10495/21982 |
dc.language.iso.spa.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
J Cancer Mol |
dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
dc.rights.uri.*.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/co/ |
dc.rights.accessrights.spa.fl_str_mv |
http://purl.org/coar/access_right/c_abf2 |
dc.rights.creativecommons.spa.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/ |
dc.format.extent.spa.fl_str_mv |
10 |
dc.format.mimetype.spa.fl_str_mv |
application/pdf |
dc.publisher.spa.fl_str_mv |
MedUnion Press |
dc.publisher.group.spa.fl_str_mv |
Alimentación y Nutrición Humana Impacto de Componentes Alimentarios en la Salud |
dc.publisher.place.spa.fl_str_mv |
Taipei, Taiwán |
institution |
Universidad de Antioquia |
bitstream.url.fl_str_mv |
https://bibliotecadigital.udea.edu.co/bitstream/10495/21982/2/license.txt https://bibliotecadigital.udea.edu.co/bitstream/10495/21982/3/MaldonadoMaria_2009_DifferentialInductionApoptosis.pdf |
bitstream.checksum.fl_str_mv |
8a4605be74aa9ea9d79846c1fba20a33 78c8a0f90a7391103864cfaca4538108 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositorio Institucional Universidad de Antioquia |
repository.mail.fl_str_mv |
andres.perez@udea.edu.co |
_version_ |
1812173300554530816 |
spelling |
Maldonado Celis, María ElenaBousserouel, SouadGossé, FrancineMinker, CaroleLobstein, AnneliseRaul, Francis2021-08-30T16:48:52Z2021-08-30T16:48:52Z20091816-0735http://hdl.handle.net/10495/219821817-4256ABSTRACT: AIM: Procyanidins (Pcy) are oligomers of catechins and epicatechins. In order to get more insight into the apoptotic effects of Pcy, we aimed to compare the death signaling pathways triggered by Pcy in TRAIL-sensitive human colon cancer cells (SW480) and in their derived TRAIL-resistant metastatic cells (SW620). METHODS: Cell death, DR4/DR5, Bcl-2/Bax proteins, mitochondrial membrane potential, and reactive oxygen species (ROS) production were analyzed by flow cytometry. Bid and cytochrome c were studied by immunoblotting and ELISA. RESULTS: Pcy (80 µg/ml) combined with exogenous TRAIL (30 ng/ml) enhanced the apoptotic effects of TRAIL in SW480 but also in SW620 cells, indicating that Pcy sensitized SW620 cells to TRAIL treatment. Pcy up-regulated the expression of death receptors DR4/DR5 of TRAIL. Activation of caspase-8 and -3 was observed in both cell lines but caspase-9 was activated only in SW620 cells. Pcy-treated SW620 cells exhibited a decrease in Bid protein level, in Bcl-2/Bax ratio, and enhanced intracellular ROS production. These events were associated with the decrease of mitochondrial membrane potential and the release of cytochrome c into the cytosol. CONCLUSION: Our data suggest that Pcy triggers apoptosis via caspase-8 and may enhance TRAIL-induced apoptosis by activation of the extrinsic pathway in both cell lines. In the TRAIL-resistant SW620 cells, the apoptotic effects of Pcy were complex and may involve a cross-talk between the extrinsic and the mitochondrial apoptotic pathways and a Pcy-triggered intracellular ROS production favoring mitochondrial disruption. These data highlight the chemopreventive potentials of apple Pcy in colorectal cancer treatments.COL0000407COL008381110application/pdfengMedUnion PressAlimentación y Nutrición HumanaImpacto de Componentes Alimentarios en la SaludTaipei, Taiwáninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic CellsApoptosisColorectal NeoplasmsNeoplasias ColorrectalesFlavonoidsFlavonoidesTrail Making TestPrueba de Secuencia AlfanuméricaMitochondriaMitocondriasJ Cancer MolJournal of Cancer Molecules213051LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/21982/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALMaldonadoMaria_2009_DifferentialInductionApoptosis.pdfMaldonadoMaria_2009_DifferentialInductionApoptosis.pdfArtículo de investigaciónapplication/pdf1406076https://bibliotecadigital.udea.edu.co/bitstream/10495/21982/3/MaldonadoMaria_2009_DifferentialInductionApoptosis.pdf78c8a0f90a7391103864cfaca4538108MD5310495/21982oai:bibliotecadigital.udea.edu.co:10495/219822021-10-25 13:40:28.237Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.coTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo= |