Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells

ABSTRACT: AIM: Procyanidins (Pcy) are oligomers of catechins and epicatechins. In order to get more insight into the apoptotic effects of Pcy, we aimed to compare the death signaling pathways triggered by Pcy in TRAIL-sensitive human colon cancer cells (SW480) and in their derived TRAIL-resistant me...

Full description

Autores:
Maldonado Celis, María Elena
Bousserouel, Souad
Gossé, Francine
Minker, Carole
Lobstein, Annelise
Raul, Francis
Tipo de recurso:
Article of investigation
Fecha de publicación:
2009
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/21982
Acceso en línea:
http://hdl.handle.net/10495/21982
Palabra clave:
Apoptosis
Colorectal Neoplasms
Neoplasias Colorrectales
Flavonoids
Flavonoides
Trail Making Test
Prueba de Secuencia Alfanumérica
Mitochondria
Mitocondrias
Rights
openAccess
License
https://creativecommons.org/licenses/by-nc-nd/2.5/co/
id UDEA2_15c5f1284daebc27cfbca461e2e1ce22
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/21982
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells
title Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells
spellingShingle Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells
Apoptosis
Colorectal Neoplasms
Neoplasias Colorrectales
Flavonoids
Flavonoides
Trail Making Test
Prueba de Secuencia Alfanumérica
Mitochondria
Mitocondrias
title_short Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells
title_full Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells
title_fullStr Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells
title_full_unstemmed Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells
title_sort Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic Cells
dc.creator.fl_str_mv Maldonado Celis, María Elena
Bousserouel, Souad
Gossé, Francine
Minker, Carole
Lobstein, Annelise
Raul, Francis
dc.contributor.author.none.fl_str_mv Maldonado Celis, María Elena
Bousserouel, Souad
Gossé, Francine
Minker, Carole
Lobstein, Annelise
Raul, Francis
dc.subject.decs.none.fl_str_mv Apoptosis
Colorectal Neoplasms
Neoplasias Colorrectales
Flavonoids
Flavonoides
Trail Making Test
Prueba de Secuencia Alfanumérica
Mitochondria
Mitocondrias
topic Apoptosis
Colorectal Neoplasms
Neoplasias Colorrectales
Flavonoids
Flavonoides
Trail Making Test
Prueba de Secuencia Alfanumérica
Mitochondria
Mitocondrias
description ABSTRACT: AIM: Procyanidins (Pcy) are oligomers of catechins and epicatechins. In order to get more insight into the apoptotic effects of Pcy, we aimed to compare the death signaling pathways triggered by Pcy in TRAIL-sensitive human colon cancer cells (SW480) and in their derived TRAIL-resistant metastatic cells (SW620). METHODS: Cell death, DR4/DR5, Bcl-2/Bax proteins, mitochondrial membrane potential, and reactive oxygen species (ROS) production were analyzed by flow cytometry. Bid and cytochrome c were studied by immunoblotting and ELISA. RESULTS: Pcy (80 µg/ml) combined with exogenous TRAIL (30 ng/ml) enhanced the apoptotic effects of TRAIL in SW480 but also in SW620 cells, indicating that Pcy sensitized SW620 cells to TRAIL treatment. Pcy up-regulated the expression of death receptors DR4/DR5 of TRAIL. Activation of caspase-8 and -3 was observed in both cell lines but caspase-9 was activated only in SW620 cells. Pcy-treated SW620 cells exhibited a decrease in Bid protein level, in Bcl-2/Bax ratio, and enhanced intracellular ROS production. These events were associated with the decrease of mitochondrial membrane potential and the release of cytochrome c into the cytosol. CONCLUSION: Our data suggest that Pcy triggers apoptosis via caspase-8 and may enhance TRAIL-induced apoptosis by activation of the extrinsic pathway in both cell lines. In the TRAIL-resistant SW620 cells, the apoptotic effects of Pcy were complex and may involve a cross-talk between the extrinsic and the mitochondrial apoptotic pathways and a Pcy-triggered intracellular ROS production favoring mitochondrial disruption. These data highlight the chemopreventive potentials of apple Pcy in colorectal cancer treatments.
publishDate 2009
dc.date.issued.none.fl_str_mv 2009
dc.date.accessioned.none.fl_str_mv 2021-08-30T16:48:52Z
dc.date.available.none.fl_str_mv 2021-08-30T16:48:52Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.local.spa.fl_str_mv Artículo de investigación
format http://purl.org/coar/resource_type/c_2df8fbb1
status_str publishedVersion
dc.identifier.issn.none.fl_str_mv 1816-0735
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/21982
dc.identifier.eissn.none.fl_str_mv 1817-4256
identifier_str_mv 1816-0735
1817-4256
url http://hdl.handle.net/10495/21982
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv J Cancer Mol
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/co/
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.extent.spa.fl_str_mv 10
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv MedUnion Press
dc.publisher.group.spa.fl_str_mv Alimentación y Nutrición Humana
Impacto de Componentes Alimentarios en la Salud
dc.publisher.place.spa.fl_str_mv Taipei, Taiwán
institution Universidad de Antioquia
bitstream.url.fl_str_mv https://bibliotecadigital.udea.edu.co/bitstream/10495/21982/2/license.txt
https://bibliotecadigital.udea.edu.co/bitstream/10495/21982/3/MaldonadoMaria_2009_DifferentialInductionApoptosis.pdf
bitstream.checksum.fl_str_mv 8a4605be74aa9ea9d79846c1fba20a33
78c8a0f90a7391103864cfaca4538108
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
repository.mail.fl_str_mv andres.perez@udea.edu.co
_version_ 1812173300554530816
spelling Maldonado Celis, María ElenaBousserouel, SouadGossé, FrancineMinker, CaroleLobstein, AnneliseRaul, Francis2021-08-30T16:48:52Z2021-08-30T16:48:52Z20091816-0735http://hdl.handle.net/10495/219821817-4256ABSTRACT: AIM: Procyanidins (Pcy) are oligomers of catechins and epicatechins. In order to get more insight into the apoptotic effects of Pcy, we aimed to compare the death signaling pathways triggered by Pcy in TRAIL-sensitive human colon cancer cells (SW480) and in their derived TRAIL-resistant metastatic cells (SW620). METHODS: Cell death, DR4/DR5, Bcl-2/Bax proteins, mitochondrial membrane potential, and reactive oxygen species (ROS) production were analyzed by flow cytometry. Bid and cytochrome c were studied by immunoblotting and ELISA. RESULTS: Pcy (80 µg/ml) combined with exogenous TRAIL (30 ng/ml) enhanced the apoptotic effects of TRAIL in SW480 but also in SW620 cells, indicating that Pcy sensitized SW620 cells to TRAIL treatment. Pcy up-regulated the expression of death receptors DR4/DR5 of TRAIL. Activation of caspase-8 and -3 was observed in both cell lines but caspase-9 was activated only in SW620 cells. Pcy-treated SW620 cells exhibited a decrease in Bid protein level, in Bcl-2/Bax ratio, and enhanced intracellular ROS production. These events were associated with the decrease of mitochondrial membrane potential and the release of cytochrome c into the cytosol. CONCLUSION: Our data suggest that Pcy triggers apoptosis via caspase-8 and may enhance TRAIL-induced apoptosis by activation of the extrinsic pathway in both cell lines. In the TRAIL-resistant SW620 cells, the apoptotic effects of Pcy were complex and may involve a cross-talk between the extrinsic and the mitochondrial apoptotic pathways and a Pcy-triggered intracellular ROS production favoring mitochondrial disruption. These data highlight the chemopreventive potentials of apple Pcy in colorectal cancer treatments.COL0000407COL008381110application/pdfengMedUnion PressAlimentación y Nutrición HumanaImpacto de Componentes Alimentarios en la SaludTaipei, Taiwáninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/Differential Induction of Apoptosis by Apple Procyanidins in TRAIL-Sensitive Human Colon Tumor Cells and Derived TRAIL-Resistant Metastatic CellsApoptosisColorectal NeoplasmsNeoplasias ColorrectalesFlavonoidsFlavonoidesTrail Making TestPrueba de Secuencia AlfanuméricaMitochondriaMitocondriasJ Cancer MolJournal of Cancer Molecules213051LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/21982/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52ORIGINALMaldonadoMaria_2009_DifferentialInductionApoptosis.pdfMaldonadoMaria_2009_DifferentialInductionApoptosis.pdfArtículo de investigaciónapplication/pdf1406076https://bibliotecadigital.udea.edu.co/bitstream/10495/21982/3/MaldonadoMaria_2009_DifferentialInductionApoptosis.pdf78c8a0f90a7391103864cfaca4538108MD5310495/21982oai:bibliotecadigital.udea.edu.co:10495/219822021-10-25 13:40:28.237Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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