A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae)
ABSTRACT: Cytogenetics of triatomines have been a valuable biological tool for the study of evolution, taxonomy, and epidemiology of these vectors of Trypanosoma cruzi. Here we present a single microtube protocol that combines micro-centrifugation and micro-spreading, allowing high quality cytogenet...
- Autores:
-
Camargo Guerrero, Mauricio
Duque Correa, María Adelaida
Berrío, Alejandro
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2006
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/23665
- Acceso en línea:
- http://hdl.handle.net/10495/23665
- Palabra clave:
- Triatominae
Chromosomes
Cromosomas
Reduviidae
Micro-spreading
- Rights
- openAccess
- License
- http://creativecommons.org/licenses/by/2.5/co/
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| dc.title.spa.fl_str_mv |
A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae) |
| title |
A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae) |
| spellingShingle |
A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae) Triatominae Chromosomes Cromosomas Reduviidae Micro-spreading |
| title_short |
A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae) |
| title_full |
A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae) |
| title_fullStr |
A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae) |
| title_full_unstemmed |
A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae) |
| title_sort |
A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae) |
| dc.creator.fl_str_mv |
Camargo Guerrero, Mauricio Duque Correa, María Adelaida Berrío, Alejandro |
| dc.contributor.author.none.fl_str_mv |
Camargo Guerrero, Mauricio Duque Correa, María Adelaida Berrío, Alejandro |
| dc.subject.decs.none.fl_str_mv |
Triatominae Chromosomes Cromosomas Reduviidae |
| topic |
Triatominae Chromosomes Cromosomas Reduviidae Micro-spreading |
| dc.subject.proposal.spa.fl_str_mv |
Micro-spreading |
| description |
ABSTRACT: Cytogenetics of triatomines have been a valuable biological tool for the study of evolution, taxonomy, and epidemiology of these vectors of Trypanosoma cruzi. Here we present a single microtube protocol that combines micro-centrifugation and micro-spreading, allowing high quality cytogenetic preparations from male gonadal material of Rhodnius prolixus and Triatoma lecticularia. The amount of cellular scattering can be modulated, which can be useful if small aggregates of synchronous cells are desired. Moreover, a higher number of slides per gonad can be obtained with fully flattened clean chromosomal spreads with minimum overlaps, optimal for classical and modern molecular cytogenetic analyses. |
| publishDate |
2006 |
| dc.date.issued.none.fl_str_mv |
2006 |
| dc.date.accessioned.none.fl_str_mv |
2021-10-30T00:07:05Z |
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2021-10-30T00:07:05Z |
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info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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https://purl.org/redcol/resource_type/ARTCORT |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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0074-0276 |
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http://hdl.handle.net/10495/23665 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762006000300021 |
| dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
| identifier_str_mv |
0074-0276 10.1590/S0074-02762006000300021 1678-8060 |
| url |
http://hdl.handle.net/10495/23665 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Mem. Inst. Oswaldo Cruz. |
| dc.rights.spa.fl_str_mv |
info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/2.5/co/ |
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https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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http://creativecommons.org/licenses/by/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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2 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Instituto Oswaldo Cruz |
| dc.publisher.group.spa.fl_str_mv |
Biología y Control de Enfermedades Infecciosas Genética Regeneración y Cáncer |
| dc.publisher.place.spa.fl_str_mv |
Río de Janeiro, Brasil |
| institution |
Universidad de Antioquia |
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Camargo Guerrero, MauricioDuque Correa, María AdelaidaBerrío, Alejandro2021-10-30T00:07:05Z2021-10-30T00:07:05Z20060074-0276http://hdl.handle.net/10495/2366510.1590/S0074-027620060003000211678-8060ABSTRACT: Cytogenetics of triatomines have been a valuable biological tool for the study of evolution, taxonomy, and epidemiology of these vectors of Trypanosoma cruzi. Here we present a single microtube protocol that combines micro-centrifugation and micro-spreading, allowing high quality cytogenetic preparations from male gonadal material of Rhodnius prolixus and Triatoma lecticularia. The amount of cellular scattering can be modulated, which can be useful if small aggregates of synchronous cells are desired. Moreover, a higher number of slides per gonad can be obtained with fully flattened clean chromosomal spreads with minimum overlaps, optimal for classical and modern molecular cytogenetic analyses.COL0007865COL00067692application/pdfengInstituto Oswaldo CruzBiología y Control de Enfermedades InfecciosasGenética Regeneración y CáncerRío de Janeiro, Brasilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTCORTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/A micro-spreading improvement for spermatogenic chromosomes from Triatominae (Hemiptera-Reduviidae)TriatominaeChromosomesCromosomasReduviidaeMicro-spreadingMem. Inst. Oswaldo Cruz.Memórias do Instituto Oswaldo Cruz3393401013CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927http://bibliotecadigital.udea.edu.co/bitstream/10495/23665/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/23665/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53ORIGINALCamargoMauricio_2006_MicroSpreadingSpermatogenic.pdfCamargoMauricio_2006_MicroSpreadingSpermatogenic.pdfArtículo de investigaciónapplication/pdf437439http://bibliotecadigital.udea.edu.co/bitstream/10495/23665/1/CamargoMauricio_2006_MicroSpreadingSpermatogenic.pdf2364cba96a5c3ffe70b547971155c2bcMD5110495/23665oai:bibliotecadigital.udea.edu.co:10495/236652021-10-29 19:07:06.284Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |