Intestinal parasitic infection alters bacterial gut microbiota in children

ABSTRACT: The study of the burden that parasites can exert upon the bacterial gut microbiota was restricted by the available technologies and their costs. Currently, next-generation sequencing coupled with traditional methodologies allows the study of eukaryotic parasites (protozoa and helminths) an...

Full description

Autores:
Toro Londoño, Miguel Ángel
Bedoya Urrego, Katherine
García Montoya, Gisela María
Galván Díaz, Ana Luz
Alzate Restrepo, Juan Fernando
Tipo de recurso:
Article of investigation
Fecha de publicación:
2019
Institución:
Universidad de Antioquia
Repositorio:
Repositorio UdeA
Idioma:
eng
OAI Identifier:
oai:bibliotecadigital.udea.edu.co:10495/23305
Acceso en línea:
http://hdl.handle.net/10495/23305
Palabra clave:
Helmintiasis
Helminthiasis
Gusanos parásitos e intestinales
Worms, intestinal and parasitic
Niños
Children
Parásitos intestinales
Rights
openAccess
License
http://creativecommons.org/licenses/by/2.5/co/
id UDEA2_13de337d3629d9457396a7d4df77cda5
oai_identifier_str oai:bibliotecadigital.udea.edu.co:10495/23305
network_acronym_str UDEA2
network_name_str Repositorio UdeA
repository_id_str
dc.title.spa.fl_str_mv Intestinal parasitic infection alters bacterial gut microbiota in children
title Intestinal parasitic infection alters bacterial gut microbiota in children
spellingShingle Intestinal parasitic infection alters bacterial gut microbiota in children
Helmintiasis
Helminthiasis
Gusanos parásitos e intestinales
Worms, intestinal and parasitic
Niños
Children
Parásitos intestinales
title_short Intestinal parasitic infection alters bacterial gut microbiota in children
title_full Intestinal parasitic infection alters bacterial gut microbiota in children
title_fullStr Intestinal parasitic infection alters bacterial gut microbiota in children
title_full_unstemmed Intestinal parasitic infection alters bacterial gut microbiota in children
title_sort Intestinal parasitic infection alters bacterial gut microbiota in children
dc.creator.fl_str_mv Toro Londoño, Miguel Ángel
Bedoya Urrego, Katherine
García Montoya, Gisela María
Galván Díaz, Ana Luz
Alzate Restrepo, Juan Fernando
dc.contributor.author.none.fl_str_mv Toro Londoño, Miguel Ángel
Bedoya Urrego, Katherine
García Montoya, Gisela María
Galván Díaz, Ana Luz
Alzate Restrepo, Juan Fernando
dc.subject.decs.none.fl_str_mv Helmintiasis
Helminthiasis
topic Helmintiasis
Helminthiasis
Gusanos parásitos e intestinales
Worms, intestinal and parasitic
Niños
Children
Parásitos intestinales
dc.subject.lemb.none.fl_str_mv Gusanos parásitos e intestinales
Worms, intestinal and parasitic
Niños
Children
dc.subject.proposal.spa.fl_str_mv Parásitos intestinales
description ABSTRACT: The study of the burden that parasites can exert upon the bacterial gut microbiota was restricted by the available technologies and their costs. Currently, next-generation sequencing coupled with traditional methodologies allows the study of eukaryotic parasites (protozoa and helminths) and its effects on the human bacterial gut microbiota diversity. This diversity can be altered by a variety of factors such as agediet, genetics and parasitic infections among others. The disturbances of the gut microbiota have been associated with a variety of illnesses. Children population in developing countries, are especially susceptible to parasitic infections because of the lack of proper sanitation and undernutrition, allowing both, the thriving of intestinal parasites and profound alteration of the gut microbiota. In this work, we have sampled the stool of 23 children from four different children’s care-centers in Medellin, Colombia, and we have identified the eukaryotic parasites by traditional and molecular methodologies coupled with microbial profiling using 16S rDNA sequencing. This mixed methodology approach has allowed us to establish an interesting relationship between Giardia intestinalis and helminth infection, having both effects upon the bacterial gut microbiota enterotypes, causing a switch from a type I to a type II enterotype upon infection.
publishDate 2019
dc.date.issued.none.fl_str_mv 2019
dc.date.accessioned.none.fl_str_mv 2021-10-20T17:57:27Z
dc.date.available.none.fl_str_mv 2021-10-20T17:57:27Z
dc.type.spa.fl_str_mv info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.redcol.spa.fl_str_mv https://purl.org/redcol/resource_type/ART
dc.type.local.spa.fl_str_mv Artículo de investigación
format http://purl.org/coar/resource_type/c_2df8fbb1
status_str publishedVersion
dc.identifier.citation.spa.fl_str_mv Toro-Londono MA, Bedoya-Urrego K, Garcia-Montoya GM, Galvan-Diaz AL, Alzate JF. 2019. Intestinal parasitic infection alters bacterial gut microbiota in children. PeerJ 7:e6200 http://doi.org/10.7717/peerj.6200
dc.identifier.issn.none.fl_str_mv 2167-8359
dc.identifier.uri.none.fl_str_mv http://hdl.handle.net/10495/23305
dc.identifier.doi.none.fl_str_mv 10.7717/peerj.6200
identifier_str_mv Toro-Londono MA, Bedoya-Urrego K, Garcia-Montoya GM, Galvan-Diaz AL, Alzate JF. 2019. Intestinal parasitic infection alters bacterial gut microbiota in children. PeerJ 7:e6200 http://doi.org/10.7717/peerj.6200
2167-8359
10.7717/peerj.6200
url http://hdl.handle.net/10495/23305
dc.language.iso.spa.fl_str_mv eng
language eng
dc.relation.ispartofjournalabbrev.spa.fl_str_mv PeerJ
dc.rights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv http://creativecommons.org/licenses/by/2.5/co/
dc.rights.accessrights.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
dc.rights.creativecommons.spa.fl_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/co/
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.format.extent.spa.fl_str_mv 23
dc.format.mimetype.spa.fl_str_mv application/pdf
dc.publisher.spa.fl_str_mv PeerJ Inc.
dc.publisher.group.spa.fl_str_mv Grupo de Parasitología Universidad de Antioquia
Microbiología Ambiental
dc.publisher.place.spa.fl_str_mv Corte Madera, Estados Unidos
institution Universidad de Antioquia
bitstream.url.fl_str_mv http://bibliotecadigital.udea.edu.co/bitstream/10495/23305/1/MiguelToro_2019_ParasiticBacterialchildren.pdf
http://bibliotecadigital.udea.edu.co/bitstream/10495/23305/3/license.txt
http://bibliotecadigital.udea.edu.co/bitstream/10495/23305/2/license_rdf
bitstream.checksum.fl_str_mv f6933087e716e14316ac68cf5b664c62
8a4605be74aa9ea9d79846c1fba20a33
1646d1f6b96dbbbc38035efc9239ac9c
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional Universidad de Antioquia
repository.mail.fl_str_mv andres.perez@udea.edu.co
_version_ 1812173122275639296
spelling Toro Londoño, Miguel ÁngelBedoya Urrego, KatherineGarcía Montoya, Gisela MaríaGalván Díaz, Ana LuzAlzate Restrepo, Juan Fernando2021-10-20T17:57:27Z2021-10-20T17:57:27Z2019Toro-Londono MA, Bedoya-Urrego K, Garcia-Montoya GM, Galvan-Diaz AL, Alzate JF. 2019. Intestinal parasitic infection alters bacterial gut microbiota in children. PeerJ 7:e6200 http://doi.org/10.7717/peerj.62002167-8359http://hdl.handle.net/10495/2330510.7717/peerj.6200ABSTRACT: The study of the burden that parasites can exert upon the bacterial gut microbiota was restricted by the available technologies and their costs. Currently, next-generation sequencing coupled with traditional methodologies allows the study of eukaryotic parasites (protozoa and helminths) and its effects on the human bacterial gut microbiota diversity. This diversity can be altered by a variety of factors such as agediet, genetics and parasitic infections among others. The disturbances of the gut microbiota have been associated with a variety of illnesses. Children population in developing countries, are especially susceptible to parasitic infections because of the lack of proper sanitation and undernutrition, allowing both, the thriving of intestinal parasites and profound alteration of the gut microbiota. In this work, we have sampled the stool of 23 children from four different children’s care-centers in Medellin, Colombia, and we have identified the eukaryotic parasites by traditional and molecular methodologies coupled with microbial profiling using 16S rDNA sequencing. This mixed methodology approach has allowed us to establish an interesting relationship between Giardia intestinalis and helminth infection, having both effects upon the bacterial gut microbiota enterotypes, causing a switch from a type I to a type II enterotype upon infection.COL0007506COL004018923application/pdfengPeerJ Inc.Grupo de Parasitología Universidad de AntioquiaMicrobiología AmbientalCorte Madera, Estados Unidosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by/4.0/Intestinal parasitic infection alters bacterial gut microbiota in childrenHelmintiasisHelminthiasisGusanos parásitos e intestinalesWorms, intestinal and parasiticNiñosChildrenParásitos intestinalesPeerJPeerJ1247ORIGINALMiguelToro_2019_ParasiticBacterialchildren.pdfMiguelToro_2019_ParasiticBacterialchildren.pdfArtículo de investigaciónapplication/pdf5577710http://bibliotecadigital.udea.edu.co/bitstream/10495/23305/1/MiguelToro_2019_ParasiticBacterialchildren.pdff6933087e716e14316ac68cf5b664c62MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/23305/3/license.txt8a4605be74aa9ea9d79846c1fba20a33MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8927http://bibliotecadigital.udea.edu.co/bitstream/10495/23305/2/license_rdf1646d1f6b96dbbbc38035efc9239ac9cMD5210495/23305oai:bibliotecadigital.udea.edu.co:10495/233052022-01-25 15:23:42.585Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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