Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd
ABSTRACT: paratuberculosis is a slow-developing infectious disease, characterized by chronic granulomatous enterocolitis. This disease has a variable incubation period from 6 months to over 15 years, and is caused by Mycobacterium avium subsp. paratuberculosis (MAP). Its detection by direct and indi...
- Autores:
-
Correa Valencia, Nathalia María del Pilar
Ramírez Vásquez, Nicolás Fernando
Bulte, Michael
Fernández Silva, Jorge Arturo
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2017
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- eng
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/10670
- Acceso en línea:
- http://hdl.handle.net/10495/10670
- Palabra clave:
- Ensayo de Inmunoadsorción Enzimática
Enzyme-Linked Immunosorbent Assay
Técnicas de Diagnóstico Molecular
Molecular Diagnostic Techniques
Mycobacterium avium subsp. paratuberculosis
Paratuberculosis
http://aims.fao.org/aos/agrovoc/c_37851
http://aims.fao.org/aos/agrovoc/c_16452
- Rights
- openAccess
- License
- Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
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| dc.title.spa.fl_str_mv |
Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd |
| dc.title.alternative.spa.fl_str_mv |
Cultivo fecal y dos metodos de PCR en materia fecal para el diagnostico de Mycobacterium avium subsp. paratuberculosis en un hato seropositivo |
| title |
Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd |
| spellingShingle |
Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd Ensayo de Inmunoadsorción Enzimática Enzyme-Linked Immunosorbent Assay Técnicas de Diagnóstico Molecular Molecular Diagnostic Techniques Mycobacterium avium subsp. paratuberculosis Paratuberculosis http://aims.fao.org/aos/agrovoc/c_37851 http://aims.fao.org/aos/agrovoc/c_16452 |
| title_short |
Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd |
| title_full |
Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd |
| title_fullStr |
Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd |
| title_full_unstemmed |
Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd |
| title_sort |
Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd |
| dc.creator.fl_str_mv |
Correa Valencia, Nathalia María del Pilar Ramírez Vásquez, Nicolás Fernando Bulte, Michael Fernández Silva, Jorge Arturo |
| dc.contributor.author.none.fl_str_mv |
Correa Valencia, Nathalia María del Pilar Ramírez Vásquez, Nicolás Fernando Bulte, Michael Fernández Silva, Jorge Arturo |
| dc.subject.decs.none.fl_str_mv |
Ensayo de Inmunoadsorción Enzimática Enzyme-Linked Immunosorbent Assay Técnicas de Diagnóstico Molecular Molecular Diagnostic Techniques |
| topic |
Ensayo de Inmunoadsorción Enzimática Enzyme-Linked Immunosorbent Assay Técnicas de Diagnóstico Molecular Molecular Diagnostic Techniques Mycobacterium avium subsp. paratuberculosis Paratuberculosis http://aims.fao.org/aos/agrovoc/c_37851 http://aims.fao.org/aos/agrovoc/c_16452 |
| dc.subject.agrovoc.none.fl_str_mv |
Mycobacterium avium subsp. paratuberculosis Paratuberculosis |
| dc.subject.agrovocuri.none.fl_str_mv |
http://aims.fao.org/aos/agrovoc/c_37851 http://aims.fao.org/aos/agrovoc/c_16452 |
| description |
ABSTRACT: paratuberculosis is a slow-developing infectious disease, characterized by chronic granulomatous enterocolitis. This disease has a variable incubation period from 6 months to over 15 years, and is caused by Mycobacterium avium subsp. paratuberculosis (MAP). Its detection by direct and indirect diagnostic techniques has been of special interest. Objective: to report the diagnosis and detection of MAP using several diagnostic testsin a herd of the Northern region ofAntioquia, Colombia. Methods:serum samples from the study herd were analyzed, using a commercial ELISA (enzyme-linked immunosorbent assay) kit. Fecalsamples were cultured by duplicate using Herrold ́s egg yolk medium (HEYM), and analyzed by an end- point IS900-specific nested PCR protocol, and a commercial F57-real-time PCR kit. Results: eight out of 27 serum samples in the study herd resulted ELISA-positive. None of fecal samples resulted positive to HEYM culture by duplicate and none were found to be positive by F57-real-time PCR. Were found to be positive by end-point IS900-specific nested PCR.Agreement was found between ELISA and end-point IS900-specific nested PCR in one of the animals. Conclusion: the present study gives information about the agreement between direct and indirect MAP-detection techniques, using different matrixes from animals under the same husbandry conditions. |
| publishDate |
2017 |
| dc.date.issued.none.fl_str_mv |
2017 |
| dc.date.accessioned.none.fl_str_mv |
2019-03-04T13:00:00Z |
| dc.date.available.none.fl_str_mv |
2019-03-04T13:00:00Z |
| dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
| dc.type.local.spa.fl_str_mv |
Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
| dc.identifier.citation.spa.fl_str_mv |
Correa NM, Ramírez NF, Bülte M, Fernández JA. Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd. Rev Colomb Cienc Pecu. 2017; 30:101-115. |
| dc.identifier.issn.none.fl_str_mv |
0120-0690 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/10670 |
| dc.identifier.doi.none.fl_str_mv |
10.17533/udea.rccp.v30n2a02 |
| dc.identifier.eissn.none.fl_str_mv |
2256-2958 |
| identifier_str_mv |
Correa NM, Ramírez NF, Bülte M, Fernández JA. Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd. Rev Colomb Cienc Pecu. 2017; 30:101-115. 0120-0690 10.17533/udea.rccp.v30n2a02 2256-2958 |
| url |
http://hdl.handle.net/10495/10670 |
| dc.language.iso.spa.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Rev. Colomb. Cienc. Pecu. |
| dc.rights.*.fl_str_mv |
Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
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info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) https://creativecommons.org/licenses/by-nc-sa/2.5/co/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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15 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad de Antioquia, Facultad de Ciencias Agrarias |
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CENTAURO |
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Medellín, Colombia |
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Universidad de Antioquia |
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Correa Valencia, Nathalia María del PilarRamírez Vásquez, Nicolás FernandoBulte, MichaelFernández Silva, Jorge Arturo2019-03-04T13:00:00Z2019-03-04T13:00:00Z2017Correa NM, Ramírez NF, Bülte M, Fernández JA. Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd. Rev Colomb Cienc Pecu. 2017; 30:101-115.0120-0690http://hdl.handle.net/10495/1067010.17533/udea.rccp.v30n2a022256-2958ABSTRACT: paratuberculosis is a slow-developing infectious disease, characterized by chronic granulomatous enterocolitis. This disease has a variable incubation period from 6 months to over 15 years, and is caused by Mycobacterium avium subsp. paratuberculosis (MAP). Its detection by direct and indirect diagnostic techniques has been of special interest. Objective: to report the diagnosis and detection of MAP using several diagnostic testsin a herd of the Northern region ofAntioquia, Colombia. Methods:serum samples from the study herd were analyzed, using a commercial ELISA (enzyme-linked immunosorbent assay) kit. Fecalsamples were cultured by duplicate using Herrold ́s egg yolk medium (HEYM), and analyzed by an end- point IS900-specific nested PCR protocol, and a commercial F57-real-time PCR kit. Results: eight out of 27 serum samples in the study herd resulted ELISA-positive. None of fecal samples resulted positive to HEYM culture by duplicate and none were found to be positive by F57-real-time PCR. Were found to be positive by end-point IS900-specific nested PCR.Agreement was found between ELISA and end-point IS900-specific nested PCR in one of the animals. Conclusion: the present study gives information about the agreement between direct and indirect MAP-detection techniques, using different matrixes from animals under the same husbandry conditions.RESUMEN: la paratuberculosis es una enfermedad infecciosa de desarrollo lento, caracterizada por una enterocolitis granulomatosa crónica. Esta enfermedad tiene un periodo de incubación que varía entre los 6 meses hasta por más de 15 años, y es causada por Mycobacterium avium subsp. paratuberculosis (MAP). Su detección por técnicas diagnósticas directas e indirectas ha sido de interés especial. Objetivo: reportar el diagnóstico y detección de MAP utilizando varias técnicas diagnósticas en un hato de la región norte de Antioquia, Colombia. Métodos: se analizaron las muestras de suero del hato de estudio utilizando un kit comercial de ELISA (enzyme-linked immunosorbent assay). Las muestras de materia fecal fueron cultivadas por duplicado en Herrold´s egg yolk medium (HEYM), y analizadas mediante un protocolo de PCR anidado específico de IS900 y un kit comercial de PCR en tiempo real para F57. Resultados: ocho de las 27 muestras de suero resultaron positivas por ELISA. Ninguna de las muestras de materia fecal resultó positiva al cultivo en HEYM por duplicado ni por PCR en tiempo real para F57. Siete de las 27 muestras de materia fecal resultaron positivas a PCR anidado específico de IS900. Se encontró concordancia entre el resultado de ELISA y de PCR anidado específico de IS900 en uno de los animales. Conclusión: el presente estudio brinda información acerca de la concordancia entre técnicas directas e indirectas de detección de MAP, utilizando diferentes matrices a partir de animales bajo las mismas condiciones de manejoCOL000126215application/pdfengUniversidad de Antioquia, Facultad de Ciencias AgrariasCENTAUROMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/2.5/co/Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herdCultivo fecal y dos metodos de PCR en materia fecal para el diagnostico de Mycobacterium avium subsp. paratuberculosis en un hato seropositivoEnsayo de Inmunoadsorción EnzimáticaEnzyme-Linked Immunosorbent AssayTécnicas de Diagnóstico MolecularMolecular Diagnostic TechniquesMycobacterium avium subsp. paratuberculosisParatuberculosishttp://aims.fao.org/aos/agrovoc/c_37851http://aims.fao.org/aos/agrovoc/c_16452Rev. Colomb. Cienc. Pecu.Revista Colombiana de Ciencias Pecuarias302CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849https://bibliotecadigital.udea.edu.co/bitstream/10495/10670/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80https://bibliotecadigital.udea.edu.co/bitstream/10495/10670/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfLicenciaapplication/rdf+xml; charset=utf-80https://bibliotecadigital.udea.edu.co/bitstream/10495/10670/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/10670/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55ORIGINALCorreaNathalia_2016_FecalPCRMycobacteriumAvium.pdfCorreaNathalia_2016_FecalPCRMycobacteriumAvium.pdfArtículo de investigaciónapplication/pdf1584589https://bibliotecadigital.udea.edu.co/bitstream/10495/10670/1/CorreaNathalia_2016_FecalPCRMycobacteriumAvium.pdf732b7c68f5a6b0750cc4235f2365fd20MD5110495/10670oai:bibliotecadigital.udea.edu.co:10495/106702022-11-10 15:32:25.248Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |