Fecal culture and two fecal-PCR methods for the diagnosis of Mycobacterium avium subsp. paratuberculosis in a seropositive herd

ABSTRACT: paratuberculosis is a slow-developing infectious disease, characterized by chronic granulomatous enterocolitis. This disease has a variable incubation period from 6 months to over 15 years, and is caused by Mycobacterium avium subsp. paratuberculosis (MAP). Its detection by direct and indirect diagnostic techniques has been of special interest. Objective: to report the diagnosis and detection of MAP using several diagnostic testsin a herd of the Northern region ofAntioquia, Colombia. Methods:serum samples from the study herd were analyzed, using a commercial ELISA (enzyme-linked immunosorbent assay) kit. Fecalsamples were cultured by duplicate using Herrold ́s egg yolk medium (HEYM), and analyzed by an end- point IS900-specific nested PCR protocol, and a commercial F57-real-time PCR kit. Results: eight out of 27 serum samples in the study herd resulted ELISA-positive. None of fecal samples resulted positive to HEYM culture by duplicate and none were found to be positive by F57-real-time PCR. Were found to be positive by end-point IS900-specific nested PCR.Agreement was found between ELISA and end-point IS900-specific nested PCR in one of the animals. Conclusion: the present study gives information about the agreement between direct and indirect MAP-detection techniques, using different matrixes from animals under the same husbandry conditions.

• Autores: Correa Valencia, Nathalia María del Pilar; Ramírez Vásquez, Nicolás Fernando; Bulte, Michael; Fernández Silva, Jorge Arturo
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2017
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: eng
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/10670
• Acceso en línea: http://hdl.handle.net/10495/10670
• Palabra clave: Ensayo de Inmunoadsorción Enzimática; Enzyme-Linked Immunosorbent Assay; Técnicas de Diagnóstico Molecular; Molecular Diagnostic Techniques; Mycobacterium avium subsp. paratuberculosis; Paratuberculosis; http://aims.fao.org/aos/agrovoc/c_37851; http://aims.fao.org/aos/agrovoc/c_16452
• Rights: openAccess
• License: Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)