Inmunolocalizacion hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)

ABSTRACT: In Colombia the diagnostic techniques for implementing immunohistochemical biomarkers to fulfill biomonitoring of environmental contamination are limited. Objective: To implement an immunohistochemical technique to immunolocate CYP 1A in the tissue of fish, performing a subacute exposition...

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Autores:: Chaparro Gutiérrez, Jenny Jovana
Aranzazu Taborda, Diego Alfonso
Urrea, Laura
Rodríguez, Berardo de Jesús

Tipo de recurso:: Article of investigation

Fecha de publicación:: 2013

Institución:: Universidad de Antioquia

Repositorio:: Repositorio UdeA

Idioma:: spa

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network_acronym_str	UDEA2
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dc.title.spa.fl_str_mv	Inmunolocalizacion hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
dc.title.alternative.spa.fl_str_mv	Liver immunolocation of CYP P450 in an subacute toxicity experiment with Clorpirifos o,o-(3, 5, 6- trichloride -2-pyridyl phosphorotioato in young male tilapia (Oreochromis spp)
title	Inmunolocalizacion hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
spellingShingle	Inmunolocalizacion hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp) Biomarcadores Biomarkers Disrupción endocrina Endocrine disruption Inmunohistoquímica Immunohistochemistry Compuestos organofosforados Organophosphate compounds Vitelogenina Vitellogenin
title_short	Inmunolocalizacion hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
title_full	Inmunolocalizacion hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
title_fullStr	Inmunolocalizacion hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
title_full_unstemmed	Inmunolocalizacion hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
title_sort	Inmunolocalizacion hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)
dc.creator.fl_str_mv	Chaparro Gutiérrez, Jenny Jovana Aranzazu Taborda, Diego Alfonso Urrea, Laura Rodríguez, Berardo de Jesús
dc.contributor.author.none.fl_str_mv	Chaparro Gutiérrez, Jenny Jovana Aranzazu Taborda, Diego Alfonso Urrea, Laura Rodríguez, Berardo de Jesús
dc.subject.none.fl_str_mv	Biomarcadores Biomarkers Disrupción endocrina Endocrine disruption Inmunohistoquímica Immunohistochemistry Compuestos organofosforados Organophosphate compounds Vitelogenina Vitellogenin
topic	Biomarcadores Biomarkers Disrupción endocrina Endocrine disruption Inmunohistoquímica Immunohistochemistry Compuestos organofosforados Organophosphate compounds Vitelogenina Vitellogenin
description	ABSTRACT: In Colombia the diagnostic techniques for implementing immunohistochemical biomarkers to fulfill biomonitoring of environmental contamination are limited. Objective: To implement an immunohistochemical technique to immunolocate CYP 1A in the tissue of fish, performing a subacute exposition experiment of Clofopirifos in the liver of young male tilapia. Methods: the sublethal dosage was carried out in semi-static state, with a daily refill of 50% of the water volume, maintaining the corresponding concentration in each group through the addition of half of the dosage until the 28th day. The Cloropirifos concentrations for the exposition were 4, 8, and 12 μg/L in the treated group and 0.0 μg/L in the control group. Samples were taken for histopathological and immunohistochemical study. Results: A significant difference (p<0.05) for some liver lesions, confirming that it is an organ affected by low dosages of Cloropirifos. It verified that the induction of CYP 1A in hepatocyte, endothelium and biliary cells equally in exposed animals and those that weren’t exposed. From a multidimensional perspective, no statistical difference was found between the treatments for the variables under study. Conclusion: this study implemented and normalized an immunohistochemical technique for the tissue immunolocation of CYP 1A that could be used in future investigations to fulfill biomonitoring of contamination in Colombia’s hydrographic basins.
publishDate	2013
dc.date.issued.none.fl_str_mv	2013
dc.date.accessioned.none.fl_str_mv	2019-03-11T21:33:14Z
dc.date.available.none.fl_str_mv	2019-03-11T21:33:14Z
dc.type.spa.fl_str_mv	info:eu-repo/semantics/article
dc.type.coarversion.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.hasversion.spa.fl_str_mv	info:eu-repo/semantics/publishedVersion
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dc.type.local.spa.fl_str_mv	Artículo de investigación
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dc.identifier.citation.spa.fl_str_mv	Chaparro J, Aranzazu D, Urrea L, Rodríguez B. Liver immunolocation of CYP P450 in an subacute toxicity experiment with Clorpirifos o,o-(3, 5, 6- trichloride -2-pyridyl phosphorotioato in young male tilapia (Oreochromis spp). Rev CES Med Zootec. 2013; Vol 8 (2): 61-72.
dc.identifier.uri.none.fl_str_mv	http://hdl.handle.net/10495/10753
dc.identifier.eissn.none.fl_str_mv	1900-9607
dc.identifier.url.spa.fl_str_mv	https://revistas.ces.edu.co/index.php/mvz/article/view/2847
identifier_str_mv	Chaparro J, Aranzazu D, Urrea L, Rodríguez B. Liver immunolocation of CYP P450 in an subacute toxicity experiment with Clorpirifos o,o-(3, 5, 6- trichloride -2-pyridyl phosphorotioato in young male tilapia (Oreochromis spp). Rev CES Med Zootec. 2013; Vol 8 (2): 61-72. 1900-9607
url	http://hdl.handle.net/10495/10753 https://revistas.ces.edu.co/index.php/mvz/article/view/2847
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.ispartofjournalabbrev.spa.fl_str_mv	Ces. Med. Vet. Zoote.
dc.rights.*.fl_str_mv	Atribución-NoComercial-SinDerivadas 2.5 Colombia
dc.rights.spa.fl_str_mv	info:eu-repo/semantics/openAccess
dc.rights.uri.*.fl_str_mv	http://creativecommons.org/licenses/by-nc-nd/2.5/co/
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dc.rights.creativecommons.spa.fl_str_mv	https://creativecommons.org/licenses/by-nc-nd/4.0/
rights_invalid_str_mv	Atribución-NoComercial-SinDerivadas 2.5 Colombia http://creativecommons.org/licenses/by-nc-nd/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv	openAccess
dc.format.mimetype.spa.fl_str_mv	application/pdf
dc.publisher.spa.fl_str_mv	Universidad CES, Facultad de Medicina Veterinaria y Zootecnia
dc.publisher.group.spa.fl_str_mv	Grupo de Investigación en Patobiología - QUIRÓN
dc.publisher.place.spa.fl_str_mv	Medellín, Colombia
institution	Universidad de Antioquia
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spelling	Chaparro Gutiérrez, Jenny JovanaAranzazu Taborda, Diego AlfonsoUrrea, LauraRodríguez, Berardo de Jesús2019-03-11T21:33:14Z2019-03-11T21:33:14Z2013Chaparro J, Aranzazu D, Urrea L, Rodríguez B. Liver immunolocation of CYP P450 in an subacute toxicity experiment with Clorpirifos o,o-(3, 5, 6- trichloride -2-pyridyl phosphorotioato in young male tilapia (Oreochromis spp). Rev CES Med Zootec. 2013; Vol 8 (2): 61-72.http://hdl.handle.net/10495/107531900-9607https://revistas.ces.edu.co/index.php/mvz/article/view/2847ABSTRACT: In Colombia the diagnostic techniques for implementing immunohistochemical biomarkers to fulfill biomonitoring of environmental contamination are limited. Objective: To implement an immunohistochemical technique to immunolocate CYP 1A in the tissue of fish, performing a subacute exposition experiment of Clofopirifos in the liver of young male tilapia. Methods: the sublethal dosage was carried out in semi-static state, with a daily refill of 50% of the water volume, maintaining the corresponding concentration in each group through the addition of half of the dosage until the 28th day. The Cloropirifos concentrations for the exposition were 4, 8, and 12 μg/L in the treated group and 0.0 μg/L in the control group. Samples were taken for histopathological and immunohistochemical study. Results: A significant difference (p<0.05) for some liver lesions, confirming that it is an organ affected by low dosages of Cloropirifos. It verified that the induction of CYP 1A in hepatocyte, endothelium and biliary cells equally in exposed animals and those that weren’t exposed. From a multidimensional perspective, no statistical difference was found between the treatments for the variables under study. Conclusion: this study implemented and normalized an immunohistochemical technique for the tissue immunolocation of CYP 1A that could be used in future investigations to fulfill biomonitoring of contamination in Colombia’s hydrographic basins.RESUMEN: En Colombia son escasas las técnicas de diagnóstico implementadas con biomarcadores inmunohistoquímicos para realizar biomonitoreo de contaminación ambiental. Objetivo: Implementar una técnica inmunohistoquímica para inmunolocalizar CYP 1A en tejidos de peces, luego de realizar un experimento de exposición subaguda al Clorpirifos en tilapias juveniles machos. Métodos: el experimento de dosis subletal, se realizó en un sistema semiestático, con recambio diario del 50% del volumen de agua manteniendo la concentración correspondiente en cada grupo experimental mediante la adición de la mitad de la dosis hasta el día 28. Las concentraciones de Clorpirifos para la exposición fueron 4, 8, y 12 μg/L, en los grupos tratados y 0,0 μg/L en el grupo control. Se tomaron muestras para estudio histopatológico e inmunohistoquímico. Resultados: se encontró diferencia significativa (p<0,05) para algunas lesiones en el hígado, confirmando que este es un órgano de impacto de los efectos del Clorpirifos a bajas dosis. Se verifico la inducción de CYP 1A en el hepatocitos, endotelio y celulas biliares tanto de los animales expuestos como en los no expuestos. Desde el punto de vista multidimensional no se encontró diferencia estadística entre los tratamientos para las variables estudiadas. Conclusión: se implementó y normalizó una técnica de inmunohistoquímica para la inmunolocalización tisular de CYP 1A, que podrá utilizarse en futuras investigaciones para realizar biomonitoreo de contaminación en las cuencas hidrográficas de Colombia.COL0092964application/pdfspaUniversidad CES, Facultad de Medicina Veterinaria y ZootecniaGrupo de Investigación en Patobiología - QUIRÓNMedellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-SinDerivadas 2.5 Colombiainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-nd/4.0/BiomarcadoresBiomarkersDisrupción endocrinaEndocrine disruptionInmunohistoquímicaImmunohistochemistryCompuestos organofosforadosOrganophosphate compoundsVitelogeninaVitellogeninInmunolocalizacion hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)Liver immunolocation of CYP P450 in an subacute toxicity experiment with Clorpirifos o,o-(3, 5, 6- trichloride -2-pyridyl phosphorotioato in young male tilapia (Oreochromis spp)Ces. Med. Vet. Zoote.Revista CES Medicina Veterinaria y Zootecnia82CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849https://bibliotecadigital.udea.edu.co/bitstream/10495/10753/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80https://bibliotecadigital.udea.edu.co/bitstream/10495/10753/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfLicenciaapplication/rdf+xml; charset=utf-80https://bibliotecadigital.udea.edu.co/bitstream/10495/10753/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://bibliotecadigital.udea.edu.co/bitstream/10495/10753/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD55ORIGINALChaparroJenny_2013_ToxicidadClorpirifosTilapiaMacho.pdfChaparroJenny_2013_ToxicidadClorpirifosTilapiaMacho.pdfArtículo de investigaciónapplication/pdf1058006https://bibliotecadigital.udea.edu.co/bitstream/10495/10753/1/ChaparroJenny_2013_ToxicidadClorpirifosTilapiaMacho.pdf5c8f01999f5487ec45a5d0c503570a52MD5110495/10753oai:bibliotecadigital.udea.edu.co:10495/107532022-10-28 14:54:52.353Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.coTk9URTogUExBQ0UgWU9VUiBPV04gTElDRU5TRSBIRVJFClRoaXMgc2FtcGxlIGxpY2Vuc2UgaXMgcHJvdmlkZWQgZm9yIGluZm9ybWF0aW9uYWwgcHVycG9zZXMgb25seS4KCk5PTi1FWENMVVNJVkUgRElTVFJJQlVUSU9OIExJQ0VOU0UKCkJ5IHNpZ25pbmcgYW5kIHN1Ym1pdHRpbmcgdGhpcyBsaWNlbnNlLCB5b3UgKHRoZSBhdXRob3Iocykgb3IgY29weXJpZ2h0Cm93bmVyKSBncmFudHMgdG8gRFNwYWNlIFVuaXZlcnNpdHkgKERTVSkgdGhlIG5vbi1leGNsdXNpdmUgcmlnaHQgdG8gcmVwcm9kdWNlLAp0cmFuc2xhdGUgKGFzIGRlZmluZWQgYmVsb3cpLCBhbmQvb3IgZGlzdHJpYnV0ZSB5b3VyIHN1Ym1pc3Npb24gKGluY2x1ZGluZwp0aGUgYWJzdHJhY3QpIHdvcmxkd2lkZSBpbiBwcmludCBhbmQgZWxlY3Ryb25pYyBmb3JtYXQgYW5kIGluIGFueSBtZWRpdW0sCmluY2x1ZGluZyBidXQgbm90IGxpbWl0ZWQgdG8gYXVkaW8gb3IgdmlkZW8uCgpZb3UgYWdyZWUgdGhhdCBEU1UgbWF5LCB3aXRob3V0IGNoYW5naW5nIHRoZSBjb250ZW50LCB0cmFuc2xhdGUgdGhlCnN1Ym1pc3Npb24gdG8gYW55IG1lZGl1bSBvciBmb3JtYXQgZm9yIHRoZSBwdXJwb3NlIG9mIHByZXNlcnZhdGlvbi4KCllvdSBhbHNvIGFncmVlIHRoYXQgRFNVIG1heSBrZWVwIG1vcmUgdGhhbiBvbmUgY29weSBvZiB0aGlzIHN1Ym1pc3Npb24gZm9yCnB1cnBvc2VzIG9mIHNlY3VyaXR5LCBiYWNrLXVwIGFuZCBwcmVzZXJ2YXRpb24uCgpZb3UgcmVwcmVzZW50IHRoYXQgdGhlIHN1Ym1pc3Npb24gaXMgeW91ciBvcmlnaW5hbCB3b3JrLCBhbmQgdGhhdCB5b3UgaGF2ZQp0aGUgcmlnaHQgdG8gZ3JhbnQgdGhlIHJpZ2h0cyBjb250YWluZWQgaW4gdGhpcyBsaWNlbnNlLiBZb3UgYWxzbyByZXByZXNlbnQKdGhhdCB5b3VyIHN1Ym1pc3Npb24gZG9lcyBub3QsIHRvIHRoZSBiZXN0IG9mIHlvdXIga25vd2xlZGdlLCBpbmZyaW5nZSB1cG9uCmFueW9uZSdzIGNvcHlyaWdodC4KCklmIHRoZSBzdWJtaXNzaW9uIGNvbnRhaW5zIG1hdGVyaWFsIGZvciB3aGljaCB5b3UgZG8gbm90IGhvbGQgY29weXJpZ2h0LAp5b3UgcmVwcmVzZW50IHRoYXQgeW91IGhhdmUgb2J0YWluZWQgdGhlIHVucmVzdHJpY3RlZCBwZXJtaXNzaW9uIG9mIHRoZQpjb3B5cmlnaHQgb3duZXIgdG8gZ3JhbnQgRFNVIHRoZSByaWdodHMgcmVxdWlyZWQgYnkgdGhpcyBsaWNlbnNlLCBhbmQgdGhhdApzdWNoIHRoaXJkLXBhcnR5IG93bmVkIG1hdGVyaWFsIGlzIGNsZWFybHkgaWRlbnRpZmllZCBhbmQgYWNrbm93bGVkZ2VkCndpdGhpbiB0aGUgdGV4dCBvciBjb250ZW50IG9mIHRoZSBzdWJtaXNzaW9uLgoKSUYgVEhFIFNVQk1JU1NJT04gSVMgQkFTRUQgVVBPTiBXT1JLIFRIQVQgSEFTIEJFRU4gU1BPTlNPUkVEIE9SIFNVUFBPUlRFRApCWSBBTiBBR0VOQ1kgT1IgT1JHQU5JWkFUSU9OIE9USEVSIFRIQU4gRFNVLCBZT1UgUkVQUkVTRU5UIFRIQVQgWU9VIEhBVkUKRlVMRklMTEVEIEFOWSBSSUdIVCBPRiBSRVZJRVcgT1IgT1RIRVIgT0JMSUdBVElPTlMgUkVRVUlSRUQgQlkgU1VDSApDT05UUkFDVCBPUiBBR1JFRU1FTlQuCgpEU1Ugd2lsbCBjbGVhcmx5IGlkZW50aWZ5IHlvdXIgbmFtZShzKSBhcyB0aGUgYXV0aG9yKHMpIG9yIG93bmVyKHMpIG9mIHRoZQpzdWJtaXNzaW9uLCBhbmQgd2lsbCBub3QgbWFrZSBhbnkgYWx0ZXJhdGlvbiwgb3RoZXIgdGhhbiBhcyBhbGxvd2VkIGJ5IHRoaXMKbGljZW5zZSwgdG8geW91ciBzdWJtaXNzaW9uLgo=

Inmunolocalizacion hepática de CYP P450 en un experimento de toxicidad subaguda por Clorpirifos 0,0-dietil 0-(3, 5, 6-tricloro-2-piridil fosforotioato) en machos juveniles de tilapia (Oreochromis spp)

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