Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia
ABSTRACT: Gestational malaria affects both the mother and the development of her embryo or fetus. Rapid diagnosis and timely and effective treatment are required to prevent complications and deaths. Objective: To compare thick blood smear with nested PCR and real-time PCR (qRT-PCR) for the diagnosis...
- Autores:
-
Blanquiceth Melendez, Yurany Patricia
Murillo Gómez, Óscar Javier
Maestre Buitrago, Amanda Elena
Corredor Rodríguez, Mauricio
- Tipo de recurso:
- Article of investigation
- Fecha de publicación:
- 2014
- Institución:
- Universidad de Antioquia
- Repositorio:
- Repositorio UdeA
- Idioma:
- spa
- OAI Identifier:
- oai:bibliotecadigital.udea.edu.co:10495/12978
- Acceso en línea:
- http://hdl.handle.net/10495/12978
- Palabra clave:
- Embarazo
Gota Gruesa
Malaria
PCR en Tiempo Real y Cuantitativa (qRT-PCR)
Plasmodium Falciparum
Plasmodium Vivax
- Rights
- openAccess
- License
- Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)
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Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia |
| dc.title.alternative.spa.fl_str_mv |
Detection of submicroscopic infection with Plasmodium spp., using classical and molecular techniques in pregnant patients from Córdoba, Colombia |
| title |
Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia |
| spellingShingle |
Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia Embarazo Gota Gruesa Malaria PCR en Tiempo Real y Cuantitativa (qRT-PCR) Plasmodium Falciparum Plasmodium Vivax |
| title_short |
Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia |
| title_full |
Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia |
| title_fullStr |
Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia |
| title_full_unstemmed |
Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia |
| title_sort |
Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia |
| dc.creator.fl_str_mv |
Blanquiceth Melendez, Yurany Patricia Murillo Gómez, Óscar Javier Maestre Buitrago, Amanda Elena Corredor Rodríguez, Mauricio |
| dc.contributor.author.none.fl_str_mv |
Blanquiceth Melendez, Yurany Patricia Murillo Gómez, Óscar Javier Maestre Buitrago, Amanda Elena Corredor Rodríguez, Mauricio |
| dc.subject.none.fl_str_mv |
Embarazo Gota Gruesa Malaria PCR en Tiempo Real y Cuantitativa (qRT-PCR) Plasmodium Falciparum Plasmodium Vivax |
| topic |
Embarazo Gota Gruesa Malaria PCR en Tiempo Real y Cuantitativa (qRT-PCR) Plasmodium Falciparum Plasmodium Vivax |
| description |
ABSTRACT: Gestational malaria affects both the mother and the development of her embryo or fetus. Rapid diagnosis and timely and effective treatment are required to prevent complications and deaths. Objective: To compare thick blood smear with nested PCR and real-time PCR (qRT-PCR) for the diagnosis of submicroscopic infections with Plasmodium falciparum and P. vivax. Methodology: 21 women with clinical manifestations of malaria, including both pregnant and non-pregnant, were studied in Puerto Libertador, Córdoba, Colombia. Peripheral blood specimens were obtained from all of them; umbilical cord and placenta blood specimens were taken in the pregnant ones. DNA was extracted and amplified for nested PCR or qRT-PCR. Statistical analysis was done using Graphpad PRISM and EPIDAT softwares. Results: The three techniques were satisfactory for the detection of Plasmodium falciparum and P. vivax in peripheral blood and in the umbilical cord and placenta specimens. Molecular tests were 100% sensitive and specific. Two submicroscopic cases of P. falciparum infection were detected with the two PCR techniques. Conclusion: qRT-PCR is advantageous over nested PCR because its standardization is shorter, it requires lesser infrastructure and it allows the quantification of DNA. |
| publishDate |
2014 |
| dc.date.issued.none.fl_str_mv |
2014 |
| dc.date.accessioned.none.fl_str_mv |
2020-01-10T16:51:33Z |
| dc.date.available.none.fl_str_mv |
2020-01-10T16:51:33Z |
| dc.type.spa.fl_str_mv |
info:eu-repo/semantics/article |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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info:eu-repo/semantics/publishedVersion |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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https://purl.org/redcol/resource_type/ART |
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Artículo de investigación |
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http://purl.org/coar/resource_type/c_2df8fbb1 |
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publishedVersion |
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Blanquiceth Melendez YP, Murillo Gómez OJ, Maestre Buitrago AE, Corredor Rodríguez M. Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia. Iatreia. 2014 Jul-Sep;27(3):277-289 |
| dc.identifier.issn.none.fl_str_mv |
0121-0793 |
| dc.identifier.uri.none.fl_str_mv |
http://hdl.handle.net/10495/12978 |
| dc.identifier.eissn.none.fl_str_mv |
2011-7965 |
| identifier_str_mv |
Blanquiceth Melendez YP, Murillo Gómez OJ, Maestre Buitrago AE, Corredor Rodríguez M. Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia. Iatreia. 2014 Jul-Sep;27(3):277-289 0121-0793 2011-7965 |
| url |
http://hdl.handle.net/10495/12978 |
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spa |
| language |
spa |
| dc.relation.ispartofjournalabbrev.spa.fl_str_mv |
Iatreia |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) |
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info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-sa/2.5/co/ |
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Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO) https://creativecommons.org/licenses/by-nc-sa/2.5/co/ http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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openAccess |
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12 |
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application/pdf |
| dc.publisher.spa.fl_str_mv |
Universidad de Antioquia, Facultad de Medicina |
| dc.publisher.group.spa.fl_str_mv |
GEBIOMIC (Genética y Bioquímica de Microorganismos) |
| dc.publisher.place.spa.fl_str_mv |
Medellín, Colombia |
| institution |
Universidad de Antioquia |
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andres.perez@udea.edu.co |
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Blanquiceth Melendez, Yurany PatriciaMurillo Gómez, Óscar JavierMaestre Buitrago, Amanda ElenaCorredor Rodríguez, Mauricio2020-01-10T16:51:33Z2020-01-10T16:51:33Z2014Blanquiceth Melendez YP, Murillo Gómez OJ, Maestre Buitrago AE, Corredor Rodríguez M. Detección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, Colombia. Iatreia. 2014 Jul-Sep;27(3):277-2890121-0793http://hdl.handle.net/10495/129782011-7965ABSTRACT: Gestational malaria affects both the mother and the development of her embryo or fetus. Rapid diagnosis and timely and effective treatment are required to prevent complications and deaths. Objective: To compare thick blood smear with nested PCR and real-time PCR (qRT-PCR) for the diagnosis of submicroscopic infections with Plasmodium falciparum and P. vivax. Methodology: 21 women with clinical manifestations of malaria, including both pregnant and non-pregnant, were studied in Puerto Libertador, Córdoba, Colombia. Peripheral blood specimens were obtained from all of them; umbilical cord and placenta blood specimens were taken in the pregnant ones. DNA was extracted and amplified for nested PCR or qRT-PCR. Statistical analysis was done using Graphpad PRISM and EPIDAT softwares. Results: The three techniques were satisfactory for the detection of Plasmodium falciparum and P. vivax in peripheral blood and in the umbilical cord and placenta specimens. Molecular tests were 100% sensitive and specific. Two submicroscopic cases of P. falciparum infection were detected with the two PCR techniques. Conclusion: qRT-PCR is advantageous over nested PCR because its standardization is shorter, it requires lesser infrastructure and it allows the quantification of DNA.RESUMEN: La malaria gestacional afecta a las madres y al embrión o feto en desarrollo; requiere diagnóstico rápido y tratamiento oportuno y efectivo para evitar las complicaciones y muertes. Objetivo: comparar las técnicas de gota gruesa, PCR anidada y PCR en tiempo real (qRT-PCR), para diagnosticar infecciones submicroscópicas por Plasmodium falciparum y P. vivax. Metodología: se estudiaron 21 mujeres con manifestaciones clínicas de malaria, incluyendo gestantes y no gestantes, en Puerto Libertador, Córdoba, Colombia; de todas se obtuvieron muestras de sangre periférica y, en las gestantes, de placenta y cordón umbilical. Se extrajo el ADN y se lo amplificó por PCR anidada y cuantitativa (qRT-PCR). Para el análisis estadístico se usaron los programas Graphpad PRISM y EPIDAT. Resultados: las tres técnicas diagnosticaron satisfactoriamente la presencia de P. falciparum y P. vivax en sangre periférica, cordón y placenta. Las pruebas moleculares presentaron sensibilidad y especificidad del 100%; dos casos de infección por P. falciparum no identificados por gota gruesa (submicroscópicos) se diagnosticaron con las dos técnicas de PCR. Conclusión: la qRT-PCR es ventajosa en comparación con la PCR anidada porque su estandarización es más corta, requiere menos infraestructura y permite cuantificar el ADN.12application/pdfspaUniversidad de Antioquia, Facultad de MedicinaGEBIOMIC (Genética y Bioquímica de Microorganismos)Medellín, Colombiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_2df8fbb1https://purl.org/redcol/resource_type/ARTArtículo de investigaciónhttp://purl.org/coar/version/c_970fb48d4fbd8a85Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/co/http://purl.org/coar/access_right/c_abf2https://creativecommons.org/licenses/by-nc-sa/4.0/EmbarazoGota GruesaMalariaPCR en Tiempo Real y Cuantitativa (qRT-PCR)Plasmodium FalciparumPlasmodium VivaxDetección de casos submicroscópicos de Plasmodium spp. utilizando técnicas clásicas y moleculares en pacientes gestantes de Córdoba, ColombiaDetection of submicroscopic infection with Plasmodium spp., using classical and molecular techniques in pregnant patients from Córdoba, ColombiaIatreiaIatreia277289273ORIGINALBlanquicethYurany_2014_CasosSubmicroscopicosPlasmodiumspp.pdfBlanquicethYurany_2014_CasosSubmicroscopicosPlasmodiumspp.pdfArtículo de investigaciónapplication/pdf1020503http://bibliotecadigital.udea.edu.co/bitstream/10495/12978/1/BlanquicethYurany_2014_CasosSubmicroscopicosPlasmodiumspp.pdf9333e32664707b5eca464d4ee7a99f7dMD51CC-LICENSElicense_urllicense_urltext/plain; charset=utf-849http://bibliotecadigital.udea.edu.co/bitstream/10495/12978/2/license_url4afdbb8c545fd630ea7db775da747b2fMD52license_textlicense_texttext/html; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/12978/3/license_textd41d8cd98f00b204e9800998ecf8427eMD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-80http://bibliotecadigital.udea.edu.co/bitstream/10495/12978/4/license_rdfd41d8cd98f00b204e9800998ecf8427eMD54LICENSElicense.txtlicense.txttext/plain; charset=utf-81748http://bibliotecadigital.udea.edu.co/bitstream/10495/12978/5/license.txt8a4605be74aa9ea9d79846c1fba20a33MD5510495/12978oai:bibliotecadigital.udea.edu.co:10495/129782021-04-03 16:31:44.198Repositorio Institucional Universidad de Antioquiaandres.perez@udea.edu.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 |