Selección, cultivo y agrupamiento de embriones bovinos

ABSTRACT: In vitro production of embryos (1VP) is a well established methodology, nevertheless the ratio of morulae (M) and blastocyst (B) obtained from fertilized oocytes remains low (<30%). The aim of this study was to evaluate the effect of grouping the embryos based on their development at 72 hours postinsemination (hpi) and their culture in fresh or old medium. The collection, maturation, fertilization and culture were performed as reported by Camargo (4). At 72 hpi, embryos were divided into four groups according to their development (2, 3-4,5-8,9-16 cells) and cultured for other 96 hours. Some cultures were performed in the same medium and others in a new one. The proportion of M and B was recorded. Our results show the beneficial effects of selection (p<0.05). Microdrops with unselected zygotes were used as control. Advanced stage embryos (5-8 and 9-16 cells) show better developmental rates. The medium being new or old had not effect on the development. We propose as a culture strategy to discard slow-cleaving embryos at 72 hpi to improve the efficiency of the IVP practice. Our results open an exciting field of research in the early development and kinetics of embryos. It could be possible that fast-cleaving embryos produce factor(s) that promote development and/or slow cleaving embryos produce toxic factors deleterious for the other embryos.

• Autores: Sierra Mondragón, Rosa Amparo; Berdugo Gutiérrez, Jesús Alfredo; Echavarría Sanchez, Hernán; Olivera Ángel, Martha
• Tipo de recurso: Article of investigation
• Fecha de publicación: 2000
• Institución: Universidad de Antioquia
• Repositorio: Repositorio UdeA
• Idioma: spa
• OAI Identifier: oai:bibliotecadigital.udea.edu.co:10495/7289
• Acceso en línea: http://hdl.handle.net/10495/7289
• Palabra clave: Desarrollo embrionario; Embriones; Producción in vitro; Embryonic developement; In vitro production; Fertilización in vitro; Bovinos
• Rights: openAccess
• License: Atribución-NoComercial-CompartirIgual 2.5 Colombia (CC BY-NC-SA 2.5 CO)