Dirofilaria immitis en perros domésticos de la ciudad de Barranquilla: aspectos epidemiológicos y clínicos

En la actualidad, las enfermedades infecciosas y enfermedades zoonóticas son en su mayor proporción el resultado o causa de enfermedades transmitidas por vectores. La dirofilariasis es una enfermedad zoonótica causada por el nematodo Dirofilaria immitis, el cual afecta perros, gatos y humanos que ha...

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Autores:
Lavalle Galvis, Rodrigo José de
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Fecha de publicación:
2024
Institución:
Universidad de Córdoba
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Repositorio Institucional Unicórdoba
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https://repositorio.unicordoba.edu.co/handle/ucordoba/8076
https://repositorio.unicordoba.edu.co
Palabra clave:
Dirofilariosis
Salud pública
Zoonosis
Diagnóstico molecular
Mosquito
Heartworm
Public health
Zoonosis
Molecular diagnosis
Mosquito
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openAccess
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Copyright Universidad de Córdoba, 2024
id UCORDOBA2_8d9fc23b66fbd150db7a92eca7167665
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network_acronym_str UCORDOBA2
network_name_str Repositorio Institucional Unicórdoba
repository_id_str
dc.title.none.fl_str_mv Dirofilaria immitis en perros domésticos de la ciudad de Barranquilla: aspectos epidemiológicos y clínicos
title Dirofilaria immitis en perros domésticos de la ciudad de Barranquilla: aspectos epidemiológicos y clínicos
spellingShingle Dirofilaria immitis en perros domésticos de la ciudad de Barranquilla: aspectos epidemiológicos y clínicos
Dirofilariosis
Salud pública
Zoonosis
Diagnóstico molecular
Mosquito
Heartworm
Public health
Zoonosis
Molecular diagnosis
Mosquito
title_short Dirofilaria immitis en perros domésticos de la ciudad de Barranquilla: aspectos epidemiológicos y clínicos
title_full Dirofilaria immitis en perros domésticos de la ciudad de Barranquilla: aspectos epidemiológicos y clínicos
title_fullStr Dirofilaria immitis en perros domésticos de la ciudad de Barranquilla: aspectos epidemiológicos y clínicos
title_full_unstemmed Dirofilaria immitis en perros domésticos de la ciudad de Barranquilla: aspectos epidemiológicos y clínicos
title_sort Dirofilaria immitis en perros domésticos de la ciudad de Barranquilla: aspectos epidemiológicos y clínicos
dc.creator.fl_str_mv Lavalle Galvis, Rodrigo José de
dc.contributor.advisor.none.fl_str_mv Oviedo Socarrás, Teresa
Oviedo, Misael
Badillo Viloria, María Auxiliadora
dc.contributor.author.none.fl_str_mv Lavalle Galvis, Rodrigo José de
dc.contributor.jury.none.fl_str_mv Tique Salleg, Vaneza Paulin
Flórez Muños, Ángel Alberto
dc.subject.proposal.spa.fl_str_mv Dirofilariosis
Salud pública
Zoonosis
Diagnóstico molecular
Mosquito
topic Dirofilariosis
Salud pública
Zoonosis
Diagnóstico molecular
Mosquito
Heartworm
Public health
Zoonosis
Molecular diagnosis
Mosquito
dc.subject.keywords.eng.fl_str_mv Heartworm
Public health
Zoonosis
Molecular diagnosis
Mosquito
description En la actualidad, las enfermedades infecciosas y enfermedades zoonóticas son en su mayor proporción el resultado o causa de enfermedades transmitidas por vectores. La dirofilariasis es una enfermedad zoonótica causada por el nematodo Dirofilaria immitis, el cual afecta perros, gatos y humanos que habitan zonas tropicales y templadas a nivel mundial con condiciones climáticas de altas temperaturas y humedad. La dirofilariasis canina está ampliamente distribuida a nivel mundial y es favorecida por factores ambientales como temperatura y humedad, siendo predominante en regiones de climas cálidos, tropicales y subtropicales (Simon et al. 2012). Se compararon diferentes métodos de diagnóstico para la dirofilariosis en caninos infectados naturalmente con D. immits , 59 perros fueron positivos por PCR, 59 fueron positivos por test de Knott modificado (TKM), 80 perros fueron positivos a TKM-PCR, 96 fueron positivos a la prueba de antígenos sin tratamiento térmico (TIM) y 104 perros fueron positivos con la prueba de antígenos con tratamiento térmico (TIM-TT). La sensibilidad y especificidad de TKM fue 53,0% y 92,0%, respectivamente; para PCR fue 45,0% y 84,0%, para TIM fue 92,0% y 99,0%, y para TIM-TT 95.2% y 99% respectivamente. El índice de Youden determinó el mejor punto de corte entre sensibilidad y especificidad para TIM-TT (0,94); seguido de TKM (0,55) y PCR (0,34). Kappa de Cohen estimó bajo desempeño para PCR, moderado desempeños para TKM y buen desempeño para TIM. La prevalencia real de D. immitis en los perros fue de 55,8%.
publishDate 2024
dc.date.accessioned.none.fl_str_mv 2024-01-23T20:54:58Z
dc.date.available.none.fl_str_mv 2024-01-23T20:54:58Z
dc.date.issued.none.fl_str_mv 2024-01-03
dc.type.none.fl_str_mv Trabajo de grado - Maestría
dc.type.driver.none.fl_str_mv info:eu-repo/semantics/masterThesis
dc.type.version.none.fl_str_mv info:eu-repo/semantics/acceptedVersion
dc.type.content.none.fl_str_mv Text
dc.type.redcol.none.fl_str_mv http://purl.org/redcol/resource_type/TM
status_str acceptedVersion
dc.identifier.uri.none.fl_str_mv https://repositorio.unicordoba.edu.co/handle/ucordoba/8076
dc.identifier.instname.none.fl_str_mv Universidad de Córdoba
dc.identifier.reponame.none.fl_str_mv Repositorio Universidad de Córdoba
dc.identifier.repourl.none.fl_str_mv https://repositorio.unicordoba.edu.co
url https://repositorio.unicordoba.edu.co/handle/ucordoba/8076
https://repositorio.unicordoba.edu.co
identifier_str_mv Universidad de Córdoba
Repositorio Universidad de Córdoba
dc.language.iso.none.fl_str_mv spa
language spa
dc.relation.references.none.fl_str_mv Arcila-Quiceno VH. Registro Electrocardiográfico de canino positivo a Dirofilarias en la Ciudad de Barrancabermeja (Santander - Colombia). Revista Electrónica de Veterinaria. 2005 VI, 05.
Blanco JA. Geografía Física de Barranquilla. En Rodolfo Zambrano, ed. Historia generla de Barranquilla (1 Ed). Mejoras. 1997; Pag: 13-22. ISBN 958-96185-0-2.
Bowman DD, Atkins C. Heartworm biology, treatment, and control. Vet Clin North Am Small Anim Pract. 2009; 39(6): 1127-58.
Ciucă L, Musella V, Miron LD, Maurelli MP, Cringoli G, Bosco A, Rinaldi L, 2016. Geographic distribution of canine heartworm (Dirofilaria immitis) infection in stray dogs of eastern Romania Geospat Health. 2016; 11(3): 499. v
Clavert CA, Rawlings CA. Pulmonary manifestations of heartworm disease. Vet Clin North Am Small AnimPract. 1985; 15(5): 991-1009.
Danta-Torres F, Otranto D. Overview on Dirofilaria immitis in the Americas, with notes on other filarial worms infecting dogs. Vet Parasitol. 2020; 228: 109113.
Dantas-Torres F, Otranto D. Dirofilariosis in the Americas: a more virulent Dirofilaria immitis?. Parasit Vectors. 2013; 6(1):288.
De la puente M, De Lavalle R, Guzman JR, Mendoza DA, Ramos AR, De la puente M, Viloria AB. Use of biomarkers as prognostic indicators in dogs with natural heartworm. Hum Vet Med. 2018; 10(3): 99-103.
De Lavalle R, De la puente M, Oviedo TS, Lugo E, Ovallos D. Wolbachia pipientis: endosymbiont bacteria of Dirofilaria immitis and its role in feline Dirofilariosis. Hum Vet Med. 2020; 12(2): 59-64.
De Lavalle R, Martinez Cruz A, Oviedo TS. Case series: Myocarditis in 6 dogs whit Dirofilaria immitis. J Vet Med. 2018: 32(6): 2169-70.
Espinosa N, Rosero A, Villegas CL, García IC, Gaviria-Catín T, Peña A, Ferro BE, Nieto LM. Canine Filarias outbreack in southwestern Colombia: A molecular and Epidemiological study. Biochemestry. 2020; 10.
Esteban-Mendoza MV, Arcila-quceno V. Albarracín-Navas J, Hernández I, Flechas-Alarcón M, Morchon R. Current situation of the precense of Dirofilaria immitis in dogs and Humans in Bucaramanga, Colombia.Front Vet Sci. 2020; 7: 488.
Florez AA, Martines AR, Pinilla JC. Prevalence of Dirofilaria immitis in shelter dogs in Bucaramanga metropolitan area, Colombia. Vet Parasitol Reg Stud Reports. 2020; 22:100489.
Furtado AP, Melo FTV, Giese EG, Dos Santos JN. Morphological redescriptionof Dirofilaria immitis. J Parasitol. 2010; 96 (3): 499-504.
Gomez L, Alzate JG, Orozco S. Reporte de un caso de Dirofilaria immitis en un perro. Hallazgos de antigenos y confirmacion del parasito a la necropsia. Rev Colom Cienc Pecua. 2006; 19(1):70-79.
Genchi C, Kramer LH. The prevalende of Dirofilaria immiits and D. repens in the old world. Vet Parasitol. 2020; 280:108995.
Genchi M, Manguia C, Ferrari N, Loukeri S. Evaluation of a rapid immunochromatographic test for the detection of low burden Dirofilaria immitis (heartworm) in dogs and cats. Parasitol Res. 2018; 117(1): 31-34.
Genchi C, Mortarino M, Rinaldi L, Cringoli G, Traldi G, Genchi M. Changing climate and changing vector-borne disease distribution: the example of Dirofilaria in Europe.Vet Parasitol. 2011; 176 (4): 295-9.
Genchi C, Rinaldi L, Mortarino M, Genchi M, Cringoli G. Climate and Dirofilaria infection in Europe. Vet Parasitol 2005; 24, 133(2-3):137-48.
Guerrero J, Ducos J, Genchi C, Rojo F, Gomez-Bautisd M, Carvalho MV, Labarthe N, Bordinl E, Gonzalez G. Update on the distribution of Dirofilaria immitis in dogs from Southern Europe and Latin America. In: Soll, M.D. (Ed.). Proceedings of the Heartworm Symposium’92. American Heart- worm Society.1992; pp. 31–37.
Guerrero J, Genchi C, Vezzoni A, Duccos de la Hitte JD, Bussieras J, Rojo FA, Ortega LM, Rodenas A, Bulman GM, Larson MH, Labarthe N, Charles T, Bordin E. Distribution of Dirofilaria immitis in selected areas of Europe and South America. In: Otto, G.F. (Ed.), Proceedings of the Heartworm Symposium’89. American Heartworm Society. 1989,;pp: 13–18.Guerrero J, Genchi C, Vezzoni A, Duccos de la Hitte JD, Bussieras J, Rojo FA, Ortega LM, Rodenas A, Bulman GM, Larson MH, Labarthe N, Charles T, Bordin E. Distribution of Dirofilaria immitis in selected areas of Europe and South America. In: Otto, G.F. (Ed.), Proceedings of the Heartworm Symposium’89. American Heartworm Society. 1989,;pp: 13–18.
Gomez L, Alzate G, Orozco S. Reporte de un caso de Dirofilaria immitis en un perro. Hallazgo de antígenos y confirmación del parásito a la necropsia. Rev Col Cienc Pec. 2006; 19:1.Gomez L, Alzate G, Orozco S. Reporte de un caso de Dirofilaria immitis en un perro. Hallazgo de antígenos y confirmación del parásito a la necropsia. Rev Col Cienc Pec. 2006; 19:1.
Hayasaki M. Re-migration of fifth-stage juvenile Dirofilaria immitis into pulmonary arteries after subcutaneous transplantation in dogs, cats, and rabbits.J Parasitol. 1996; 82(5): 835-7. Hayasaki M. Re-migration of fifth-stage juvenile Dirofilaria immitis into pulmonary arteries after subcutaneous transplantation in dogs, cats, and rabbits.J Parasitol. 1996; 82(5): 835-7.
Hendrix CM, Wagner MJ, Bemrick WJ, Schlotthauer JC, Stromberg BE. A scanning electron microscopic study of third-stage larvae of Dirofilaria immitis. J Parasitol. 1984; 70(1): 149-51.
Kabuusu RM, Donna DF, Pinckney R, Chriestmon J, Alexander R, Richards C, Macpherson C. An analysis of time trends for canine heartworm disease in Grenada and its associated risk factors based on veterinary clinical pathology laboratory data base records between 2003 and 2015. Prev Vet Med. 2020; 179:104989. Kabuusu RM, Donna DF, Pinckney R, Chriestmon J, Alexander R, Richards C, Macpherson C. An analysis of time trends for canine heartworm disease in Grenada and its associated risk factors based on veterinary clinical pathology laboratory data base records between 2003 and 2015. Prev Vet Med. 2020; 179:104989.
Khanmohammdi M, Akhlaghi L, Razmjou E, Falak R, Zolfaghari E Mokhtarian K, Arashadi M, Tasbihi M, Reza AM. Morphological description, phylogenetic and molecular analisis of Dirofilaria immitis isolated from dogs in the northwest of Iran. Iran J Parasitol. 2020; 15(1): 57-56.
Klotins KC, Martin SW, Bonnett BN, Peregrine AS. Canine heartworm testing in Canada: are we being effective?. Can Vet J. 2000; 41(12): 929-37.
Knott J. A method for making microfilarial surveys on day blood. Trans R Soc Trop Med Hyg. 1939; 33(2): 191-96.
Kulldorff M. A spatial scan statistic. Bernoulli, Discrete Poisson and Continuous Poisson Models. Communications in Statistics. Theory Meth. 1997; 26: 1481-1496.
Labarthe N, Rodriguez N, Couto G, Mende-de-Almedia F, Guerrero J. A pilot survey of vector-transmitted diseases in Cartagena and Barranquilla Colombia. Intern J Appl Res Vet Med. 2018; 16n(1): 63-73.
Labarthe N. Guerrero J. Epidemiology of heartworm: What is happening in South America and Mexico?. Vet Parasitol. 2005; 133 (2-3); 149-56.
Lee AC, Atkins C. Understanding feline heartworm infection: disease, diagnosis, and treatment. Top Companion Anim Med. 2010; 25 (4): 224-230.
Little M, Vaughn J, Williams L. Canine filariasis in Colombia. J Parasitol. 1968; 54:634-635.
Little S, Saleh M, Wohltjen M, Nagamori Y. Prime detection of Dirofilaria immiitis: understanding the influence of blocked antigen on heartworm test performance. Parasit Vectors. 2018; 11(1): 186.
Lok JB, Harpaz T, Knight DH. Abnormal patterns of embryogenesis in Dirofilaria immitis treated with ivermectin. J Helminthol. 1988; 62(3): 175-80.
McCall JW, Genchi C, Kramer LH, Guerrero J, Venco L. Heartworm disease in animals and humans. Adv Parasitol. 2008;66:193-285.
McCown ME, Monterroso VH, Cardona W. Surveillance for Ehrlichia canis, Anaplasma phagocytophilum, Borrelia burgdorferi, and Dirofilaria immitis in Dogs From Three Cities in Colombia. J SpeC Oper Med. 2014; 14 (1): 86-90.
Medlock JM, Barrass I, Kerrood E, Taylor Ma, Leach S. Analysis of climatic predictions for extrinsic incubation of Dirofilaria in the United kingdom. Vector Borne Zoonotic Dis. 2007; 7(1): 4-14.
Monteriro LC, Souza JR, Albuquerque CM. Eclosion rate, development and survivorship of Aedes albopictus (Skude)(Diptera: Culicidae) under different water temperatures.2007; 36(6): 966-071.
Montoya JA, Morales M, Ferrer O, Molina JM, Corbera JA. The prevalence of Dirofilaria immitis in Gran Canaria, Canary Islands, Spain (1994-1996). Vet Parasitol. 1998; 75(2-3): 221-26.
Morchón R, Carretón E, González-Miguel J, Mellado-Hernández I. Heartworm disease (Dirofilaria immitis) and their vectors in Europe-New Distribution trends. Front Physiol. 2012; 3:196.
Nelson TC, McCall JW, Jones S, Moorhead A. American Heartworm Society (AHS) 2018 Guideline Committee. 2018. Prevention, Diagnosis, and Management of Heartworm Infection in Dogs.
Orozco SC, Cardona W. Deteccion de antigenos de Dirofilaria immitis en caninos del Área metropolitana del Valle de Aburrá. Rev Colom Cienc Pec. 2006: 19 (3) :280-290. Orozco SC, Cardona W. Deteccion de antigenos de Dirofilaria immitis en caninos del Área metropolitana del Valle de Aburrá. Rev Colom Cienc Pec. 2006: 19 (3) :280-290.
Oviedo P, Lage B, Romero N, Fonseca D, Amaral H. Spatial and statistical methodologies to determine the distribution of dengue in Brazilian municipalities and relate incidence with the Health Vulnerability Index”. Spat Spatiotemporal Epidemiol. 2014; 11: 143–151.
Paes-de-Almeida, Ferreira AM, Labarthe NV, Caldas ML, Mcwall JW. Kidney ultrastructural lesions in dogs experimentally infected with Dirofilaria immitis (Leidy, 1856). Vet Parasitol. 2003; 113 (2), 157-168.
Reifur L, Thomaz-Soccol V, Montiani-Ferreira F. Epidemiological aspects of filariosis in dogs on the coast of Paraná state, Brazil: whith emphasis on Dirofilaria immitis. Vet Parasitol. 2004; 122 (4): 273-86.
Swartzentruber S, LeMonte A, Witt J, Fuller D, Davis T, Hage C, Connolly P, Durkin M, Wheat LJ. Improved detection of Histo- plasma antigenemia following dissociation of immune complexes. Clin Vacc Immunol. 2009. 16; 320–322.
Selby LA, Corwin RM, Hayes HM. Risk factors associated with canine heartworm infection. J Am Vet Med Assoc. 1980; 176(1): 33-5.
Simón F, Morchón R, González-Miguel J, Marcos-Atxutegui C, Siles-Lucas M. What is new about animal and human dirofilariosis? Trends Parasitol. 2009; 25(9): 404-9.
Simón F, Siles-Lucas M, Morchón R, Gónzales-Miguel J, Mellado I, Carretón E, Montoya-Alonso JA. Human and Animal Dirofilariasis: the Emergence of a Zoonotic Mosaic. Clin Microbiol Rev. 2012; 25 (3), 507-544.
Simon M, Genchi C. Dirofilariosis and other zoonotic filariases: an emerging public health problem in developed countries. Research and reviews in parasitology. 2000; 60 (1-2): 1-16.
Taíssa AL, Lima N, Barbosa AS, Leles D, Monteriro AB, Labarthe NV, Machado Oantunes MC. Detection of Dirofilaria immitis using microscopic, serological and molecular techniques among dogs in Cabo Frio, RJ, Brazil. Rev Bras Parasitol Vet. 2020; 29(1): 1-12.
Turba ME, Zambon E, Zannoni A, Russo S, Gentilini F. Detection of Wolbachia DNA in Blood for Diagnosing Filaria-Associated Syndromes in Cats. J Clin Microbiol. 2012; 50(8): 2624-30.
Young O, Kyung K, Joong H. Molecular detection of Dirofilaria immitis specific gene from infected dog blood sample using polymerase chain reaction. Iran J Parasitol.2017; 12(3):433-440.
Velasquez L, Blagburn BL, Duncan-Decoq R, Johson EM, Meinkoth J, Gruntmeir J, Little S. Increased prevalence of Dirofilaria immitis antigen in canine samples after heat treatment. Vet Parasitol. 2014; 206(1-2): 67.70.
Vezzani D, Eiras DF, Wisnivesky C. Dirofilariasis in Argentina: Historical review and first report of Dirofilaria immitis in a natural mosquito population. Vet Parasitol. 2006; 136 (3-4):259-73.
Vieira C, Montoya M, Agudelo S, Velez ID, Simón F. Human antibody response to a 56-kDa purified excretory/secretory product of Dirofilaria immitis. Trop Med Int Health. 2000; 5 (12): 855-859.
Vieria C, Vélez ID, Montoya MN, Agudelo S, Alvarez MI, Genchi C, Simón F. Dirofilaria immitis in Tikuna Indians and their dogs in the Colombian Amazon. Ann Trop Med Parasitol. 1998; 92(1): 123-5.
Wei L, Patrick K, Ackerson K, El.Mahallawy HS, Kaltenboeck B, Wang C. Molecular detection of Dirofilaria immitis, Hepatozoon canis, Babesia spp., Anaplasma platys and Ehrlichia canis in dogs on Costa Rica. Ata Parasitol. 2014; 60(1): 21-5.
Weil GJ, Malane MS, Powers KG. Detection of Circulating Parasite Antigens in Canine Dirofilariasis by Counterimmunoelectrophoresis. Am J Med Hyg. 1984; 33(3): 425-30.
Weil GJ, Malane MS, Powers KG, Blair LS. Monoclonal antibodies to parasite antigens found in the serum of Dirofilaria immitis-infected dogs. J. Immunol. 1985; 134: 1185–1191.
Zueva T, Morchón R, Carretón E, Ollari-Ib´ñez C, Pericacho M, Rodríguez-Barbero A, Simón F. Angiogenesis in cardiopulmonary dirofilariosis: does teh wolbachia surface protein have a pro- or anti-angiogenbic effect. J Heminthol. 2020; 10(94): 162.
dc.rights.none.fl_str_mv Copyright Universidad de Córdoba, 2024
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dc.publisher.program.none.fl_str_mv Maestría en Ciencias Veterinarias del Trópico
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spelling Oviedo Socarrás, Teresaaa5990de-e2db-4827-8428-250c66decf4e-1Oviedo, Misael5a55094d-863b-410a-9bd8-e725bfb4f228-1Badillo Viloria, María Auxiliadora7583f49b-2cfe-4ec1-8721-9adffd130998Lavalle Galvis, Rodrigo José decd87435f-67b6-4a12-9550-31b4c984c288-1Tique Salleg, Vaneza Paulina53d3295-81c9-4112-81f8-1d740ddbb059600Flórez Muños, Ángel Alberto813d7d94-a873-48a5-ac76-29b1915b38912024-01-23T20:54:58Z2024-01-23T20:54:58Z2024-01-03https://repositorio.unicordoba.edu.co/handle/ucordoba/8076Universidad de CórdobaRepositorio Universidad de Córdobahttps://repositorio.unicordoba.edu.coEn la actualidad, las enfermedades infecciosas y enfermedades zoonóticas son en su mayor proporción el resultado o causa de enfermedades transmitidas por vectores. La dirofilariasis es una enfermedad zoonótica causada por el nematodo Dirofilaria immitis, el cual afecta perros, gatos y humanos que habitan zonas tropicales y templadas a nivel mundial con condiciones climáticas de altas temperaturas y humedad. La dirofilariasis canina está ampliamente distribuida a nivel mundial y es favorecida por factores ambientales como temperatura y humedad, siendo predominante en regiones de climas cálidos, tropicales y subtropicales (Simon et al. 2012). Se compararon diferentes métodos de diagnóstico para la dirofilariosis en caninos infectados naturalmente con D. immits , 59 perros fueron positivos por PCR, 59 fueron positivos por test de Knott modificado (TKM), 80 perros fueron positivos a TKM-PCR, 96 fueron positivos a la prueba de antígenos sin tratamiento térmico (TIM) y 104 perros fueron positivos con la prueba de antígenos con tratamiento térmico (TIM-TT). La sensibilidad y especificidad de TKM fue 53,0% y 92,0%, respectivamente; para PCR fue 45,0% y 84,0%, para TIM fue 92,0% y 99,0%, y para TIM-TT 95.2% y 99% respectivamente. El índice de Youden determinó el mejor punto de corte entre sensibilidad y especificidad para TIM-TT (0,94); seguido de TKM (0,55) y PCR (0,34). Kappa de Cohen estimó bajo desempeño para PCR, moderado desempeños para TKM y buen desempeño para TIM. La prevalencia real de D. immitis en los perros fue de 55,8%.At present, infectious diseases and zoonotic diseases are mostly the result or cause of vector-borne diseases. Heartworm is a zoonotic disease caused by the Dirofilaria immitis nematode, which affects dogs, cats and humans that inhabit tropical and temperate areas worldwide with climatic conditions of high temperatures and humidity. Canine heartworm is widely distributed worldwide and is favored by environmental factors such as temperature and humidity, being predominant in regions with hot, tropical and subtropical climates (Simon et al. 2012). Different diagnostic methods for heartworm disease were compared in canines naturally infected with D. immits, 59 dogs were positive by PCR, 59 were positive by modified Knott test (TKM), 80 dogs were positive by TKM-PCR, 96 were positive by the antigen test without heat treatment (TIM) and 104 dogs were positive with the test heat treated antigens (TIM-TT). The sensitivity and specificity of TKM were 53.0% and 92.0%, respectively; for PCR it was 45.0% and 84.0%, for TIM it was 92.0% and 99.0% and for TIM-TT 95.2% y 99%, respectively. The Youden index determined the best cut-off point between sensitivity and specificity for TIM-TT (0.94); followed by TKM (0.55) and PCR (0.34). Cohen's Kappa estimated low performance for CRP, moderate performance for TKM and good performance for TIM. The actual prevalence of D. immitis in dogs was 55.8.RESUMEN GENERAL....................8ABSTRACT.....................10Palabras clave....................11Key words..................11INTRODUCCIÓN..................121. OBJETIVOS..................151.1. Objetivo General:..................151.2. Objetivos específicos:..................152. MARCO TEÓRICO..................162.1. Descripción y taxonomía..................162.2. Epidemiología..................162.3. Ciclo de biológico..................202.4. Patofisiología..................232.5. Presentación clínica..................262.6. Diagnóstico..................272.6.1.Diagnóstico microscópico: Knott modificado..................282.6.2.Diagnóstico serológico..................292.6.3.Diagnóstico molecular..................302.6.4.Diagnóstico paraclínico..................12.6.5.Diagnóstico clínico..................313.CAPÍTULO 2..................33Comparación de 3 métodos diagnósticos para la detección de infección por Dirofilaria immitis en caninos...................33Resumen..................33Abstract..................34Palabras claves:..................35Key words..................35Introducción..................35Materiales y métodos..................37Resultados..................41Discusión..................43Conclusiones..................46Referencias..................474.CAPÍTULO 3..................52Distribución de Dirofilaria immitis en perros en el área metropolitana del Caribe Colombiano y su correlación clínica y epidemiológica...................52Resumen..................52Palabras claves..................53Introducción..................53Materiales y métodos...................55Discusión...................63Conclusiones...................66Referencias...................68CONCLUSIONES GENERALES..................74RECOMENDACIONES GENERALES..................755.REFERENCIAS..................76MaestríaMagíster en Ciencias Veterinarias del TrópicoTrabajos de Investigación y/o Extensiónapplication/pdfspaUniversidad de CórdobaFacultad de Medicina Veterinaria y ZootecniaBerastegui, Córdoba, ColombiaMaestría en Ciencias Veterinarias del TrópicoCopyright Universidad de Córdoba, 2024https://creativecommons.org/licenses/by-nc-nd/4.0/Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2https://repositorio.unicordoba.edu.coDirofilaria immitis en perros domésticos de la ciudad de Barranquilla: aspectos epidemiológicos y clínicosTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMArcila-Quiceno VH. Registro Electrocardiográfico de canino positivo a Dirofilarias en la Ciudad de Barrancabermeja (Santander - Colombia). Revista Electrónica de Veterinaria. 2005 VI, 05.Blanco JA. Geografía Física de Barranquilla. En Rodolfo Zambrano, ed. Historia generla de Barranquilla (1 Ed). Mejoras. 1997; Pag: 13-22. ISBN 958-96185-0-2.Bowman DD, Atkins C. Heartworm biology, treatment, and control. Vet Clin North Am Small Anim Pract. 2009; 39(6): 1127-58.Ciucă L, Musella V, Miron LD, Maurelli MP, Cringoli G, Bosco A, Rinaldi L, 2016. Geographic distribution of canine heartworm (Dirofilaria immitis) infection in stray dogs of eastern Romania Geospat Health. 2016; 11(3): 499. vClavert CA, Rawlings CA. Pulmonary manifestations of heartworm disease. Vet Clin North Am Small AnimPract. 1985; 15(5): 991-1009.Danta-Torres F, Otranto D. Overview on Dirofilaria immitis in the Americas, with notes on other filarial worms infecting dogs. Vet Parasitol. 2020; 228: 109113.Dantas-Torres F, Otranto D. Dirofilariosis in the Americas: a more virulent Dirofilaria immitis?. Parasit Vectors. 2013; 6(1):288.De la puente M, De Lavalle R, Guzman JR, Mendoza DA, Ramos AR, De la puente M, Viloria AB. Use of biomarkers as prognostic indicators in dogs with natural heartworm. Hum Vet Med. 2018; 10(3): 99-103.De Lavalle R, De la puente M, Oviedo TS, Lugo E, Ovallos D. Wolbachia pipientis: endosymbiont bacteria of Dirofilaria immitis and its role in feline Dirofilariosis. Hum Vet Med. 2020; 12(2): 59-64.De Lavalle R, Martinez Cruz A, Oviedo TS. Case series: Myocarditis in 6 dogs whit Dirofilaria immitis. J Vet Med. 2018: 32(6): 2169-70.Espinosa N, Rosero A, Villegas CL, García IC, Gaviria-Catín T, Peña A, Ferro BE, Nieto LM. Canine Filarias outbreack in southwestern Colombia: A molecular and Epidemiological study. Biochemestry. 2020; 10.Esteban-Mendoza MV, Arcila-quceno V. Albarracín-Navas J, Hernández I, Flechas-Alarcón M, Morchon R. Current situation of the precense of Dirofilaria immitis in dogs and Humans in Bucaramanga, Colombia.Front Vet Sci. 2020; 7: 488.Florez AA, Martines AR, Pinilla JC. Prevalence of Dirofilaria immitis in shelter dogs in Bucaramanga metropolitan area, Colombia. Vet Parasitol Reg Stud Reports. 2020; 22:100489.Furtado AP, Melo FTV, Giese EG, Dos Santos JN. Morphological redescriptionof Dirofilaria immitis. J Parasitol. 2010; 96 (3): 499-504.Gomez L, Alzate JG, Orozco S. Reporte de un caso de Dirofilaria immitis en un perro. Hallazgos de antigenos y confirmacion del parasito a la necropsia. Rev Colom Cienc Pecua. 2006; 19(1):70-79.Genchi C, Kramer LH. The prevalende of Dirofilaria immiits and D. repens in the old world. Vet Parasitol. 2020; 280:108995.Genchi M, Manguia C, Ferrari N, Loukeri S. Evaluation of a rapid immunochromatographic test for the detection of low burden Dirofilaria immitis (heartworm) in dogs and cats. Parasitol Res. 2018; 117(1): 31-34.Genchi C, Mortarino M, Rinaldi L, Cringoli G, Traldi G, Genchi M. Changing climate and changing vector-borne disease distribution: the example of Dirofilaria in Europe.Vet Parasitol. 2011; 176 (4): 295-9.Genchi C, Rinaldi L, Mortarino M, Genchi M, Cringoli G. Climate and Dirofilaria infection in Europe. Vet Parasitol 2005; 24, 133(2-3):137-48.Guerrero J, Ducos J, Genchi C, Rojo F, Gomez-Bautisd M, Carvalho MV, Labarthe N, Bordinl E, Gonzalez G. Update on the distribution of Dirofilaria immitis in dogs from Southern Europe and Latin America. In: Soll, M.D. (Ed.). Proceedings of the Heartworm Symposium’92. American Heart- worm Society.1992; pp. 31–37.Guerrero J, Genchi C, Vezzoni A, Duccos de la Hitte JD, Bussieras J, Rojo FA, Ortega LM, Rodenas A, Bulman GM, Larson MH, Labarthe N, Charles T, Bordin E. Distribution of Dirofilaria immitis in selected areas of Europe and South America. In: Otto, G.F. (Ed.), Proceedings of the Heartworm Symposium’89. American Heartworm Society. 1989,;pp: 13–18.Guerrero J, Genchi C, Vezzoni A, Duccos de la Hitte JD, Bussieras J, Rojo FA, Ortega LM, Rodenas A, Bulman GM, Larson MH, Labarthe N, Charles T, Bordin E. Distribution of Dirofilaria immitis in selected areas of Europe and South America. In: Otto, G.F. (Ed.), Proceedings of the Heartworm Symposium’89. American Heartworm Society. 1989,;pp: 13–18.Gomez L, Alzate G, Orozco S. Reporte de un caso de Dirofilaria immitis en un perro. Hallazgo de antígenos y confirmación del parásito a la necropsia. Rev Col Cienc Pec. 2006; 19:1.Gomez L, Alzate G, Orozco S. Reporte de un caso de Dirofilaria immitis en un perro. Hallazgo de antígenos y confirmación del parásito a la necropsia. Rev Col Cienc Pec. 2006; 19:1.Hayasaki M. Re-migration of fifth-stage juvenile Dirofilaria immitis into pulmonary arteries after subcutaneous transplantation in dogs, cats, and rabbits.J Parasitol. 1996; 82(5): 835-7. Hayasaki M. Re-migration of fifth-stage juvenile Dirofilaria immitis into pulmonary arteries after subcutaneous transplantation in dogs, cats, and rabbits.J Parasitol. 1996; 82(5): 835-7.Hendrix CM, Wagner MJ, Bemrick WJ, Schlotthauer JC, Stromberg BE. A scanning electron microscopic study of third-stage larvae of Dirofilaria immitis. J Parasitol. 1984; 70(1): 149-51.Kabuusu RM, Donna DF, Pinckney R, Chriestmon J, Alexander R, Richards C, Macpherson C. An analysis of time trends for canine heartworm disease in Grenada and its associated risk factors based on veterinary clinical pathology laboratory data base records between 2003 and 2015. Prev Vet Med. 2020; 179:104989. Kabuusu RM, Donna DF, Pinckney R, Chriestmon J, Alexander R, Richards C, Macpherson C. An analysis of time trends for canine heartworm disease in Grenada and its associated risk factors based on veterinary clinical pathology laboratory data base records between 2003 and 2015. Prev Vet Med. 2020; 179:104989.Khanmohammdi M, Akhlaghi L, Razmjou E, Falak R, Zolfaghari E Mokhtarian K, Arashadi M, Tasbihi M, Reza AM. Morphological description, phylogenetic and molecular analisis of Dirofilaria immitis isolated from dogs in the northwest of Iran. Iran J Parasitol. 2020; 15(1): 57-56.Klotins KC, Martin SW, Bonnett BN, Peregrine AS. Canine heartworm testing in Canada: are we being effective?. Can Vet J. 2000; 41(12): 929-37.Knott J. A method for making microfilarial surveys on day blood. Trans R Soc Trop Med Hyg. 1939; 33(2): 191-96.Kulldorff M. A spatial scan statistic. Bernoulli, Discrete Poisson and Continuous Poisson Models. Communications in Statistics. Theory Meth. 1997; 26: 1481-1496.Labarthe N, Rodriguez N, Couto G, Mende-de-Almedia F, Guerrero J. A pilot survey of vector-transmitted diseases in Cartagena and Barranquilla Colombia. Intern J Appl Res Vet Med. 2018; 16n(1): 63-73.Labarthe N. Guerrero J. Epidemiology of heartworm: What is happening in South America and Mexico?. Vet Parasitol. 2005; 133 (2-3); 149-56.Lee AC, Atkins C. Understanding feline heartworm infection: disease, diagnosis, and treatment. Top Companion Anim Med. 2010; 25 (4): 224-230.Little M, Vaughn J, Williams L. Canine filariasis in Colombia. J Parasitol. 1968; 54:634-635.Little S, Saleh M, Wohltjen M, Nagamori Y. Prime detection of Dirofilaria immiitis: understanding the influence of blocked antigen on heartworm test performance. Parasit Vectors. 2018; 11(1): 186.Lok JB, Harpaz T, Knight DH. Abnormal patterns of embryogenesis in Dirofilaria immitis treated with ivermectin. J Helminthol. 1988; 62(3): 175-80.McCall JW, Genchi C, Kramer LH, Guerrero J, Venco L. Heartworm disease in animals and humans. Adv Parasitol. 2008;66:193-285.McCown ME, Monterroso VH, Cardona W. Surveillance for Ehrlichia canis, Anaplasma phagocytophilum, Borrelia burgdorferi, and Dirofilaria immitis in Dogs From Three Cities in Colombia. J SpeC Oper Med. 2014; 14 (1): 86-90.Medlock JM, Barrass I, Kerrood E, Taylor Ma, Leach S. Analysis of climatic predictions for extrinsic incubation of Dirofilaria in the United kingdom. Vector Borne Zoonotic Dis. 2007; 7(1): 4-14.Monteriro LC, Souza JR, Albuquerque CM. Eclosion rate, development and survivorship of Aedes albopictus (Skude)(Diptera: Culicidae) under different water temperatures.2007; 36(6): 966-071.Montoya JA, Morales M, Ferrer O, Molina JM, Corbera JA. The prevalence of Dirofilaria immitis in Gran Canaria, Canary Islands, Spain (1994-1996). Vet Parasitol. 1998; 75(2-3): 221-26.Morchón R, Carretón E, González-Miguel J, Mellado-Hernández I. Heartworm disease (Dirofilaria immitis) and their vectors in Europe-New Distribution trends. Front Physiol. 2012; 3:196.Nelson TC, McCall JW, Jones S, Moorhead A. American Heartworm Society (AHS) 2018 Guideline Committee. 2018. Prevention, Diagnosis, and Management of Heartworm Infection in Dogs.Orozco SC, Cardona W. Deteccion de antigenos de Dirofilaria immitis en caninos del Área metropolitana del Valle de Aburrá. Rev Colom Cienc Pec. 2006: 19 (3) :280-290. Orozco SC, Cardona W. Deteccion de antigenos de Dirofilaria immitis en caninos del Área metropolitana del Valle de Aburrá. Rev Colom Cienc Pec. 2006: 19 (3) :280-290.Oviedo P, Lage B, Romero N, Fonseca D, Amaral H. Spatial and statistical methodologies to determine the distribution of dengue in Brazilian municipalities and relate incidence with the Health Vulnerability Index”. Spat Spatiotemporal Epidemiol. 2014; 11: 143–151.Paes-de-Almeida, Ferreira AM, Labarthe NV, Caldas ML, Mcwall JW. Kidney ultrastructural lesions in dogs experimentally infected with Dirofilaria immitis (Leidy, 1856). Vet Parasitol. 2003; 113 (2), 157-168.Reifur L, Thomaz-Soccol V, Montiani-Ferreira F. Epidemiological aspects of filariosis in dogs on the coast of Paraná state, Brazil: whith emphasis on Dirofilaria immitis. Vet Parasitol. 2004; 122 (4): 273-86.Swartzentruber S, LeMonte A, Witt J, Fuller D, Davis T, Hage C, Connolly P, Durkin M, Wheat LJ. Improved detection of Histo- plasma antigenemia following dissociation of immune complexes. Clin Vacc Immunol. 2009. 16; 320–322.Selby LA, Corwin RM, Hayes HM. Risk factors associated with canine heartworm infection. J Am Vet Med Assoc. 1980; 176(1): 33-5.Simón F, Morchón R, González-Miguel J, Marcos-Atxutegui C, Siles-Lucas M. What is new about animal and human dirofilariosis? Trends Parasitol. 2009; 25(9): 404-9.Simón F, Siles-Lucas M, Morchón R, Gónzales-Miguel J, Mellado I, Carretón E, Montoya-Alonso JA. Human and Animal Dirofilariasis: the Emergence of a Zoonotic Mosaic. Clin Microbiol Rev. 2012; 25 (3), 507-544.Simon M, Genchi C. Dirofilariosis and other zoonotic filariases: an emerging public health problem in developed countries. Research and reviews in parasitology. 2000; 60 (1-2): 1-16.Taíssa AL, Lima N, Barbosa AS, Leles D, Monteriro AB, Labarthe NV, Machado Oantunes MC. Detection of Dirofilaria immitis using microscopic, serological and molecular techniques among dogs in Cabo Frio, RJ, Brazil. Rev Bras Parasitol Vet. 2020; 29(1): 1-12.Turba ME, Zambon E, Zannoni A, Russo S, Gentilini F. Detection of Wolbachia DNA in Blood for Diagnosing Filaria-Associated Syndromes in Cats. J Clin Microbiol. 2012; 50(8): 2624-30.Young O, Kyung K, Joong H. Molecular detection of Dirofilaria immitis specific gene from infected dog blood sample using polymerase chain reaction. Iran J Parasitol.2017; 12(3):433-440.Velasquez L, Blagburn BL, Duncan-Decoq R, Johson EM, Meinkoth J, Gruntmeir J, Little S. Increased prevalence of Dirofilaria immitis antigen in canine samples after heat treatment. Vet Parasitol. 2014; 206(1-2): 67.70.Vezzani D, Eiras DF, Wisnivesky C. Dirofilariasis in Argentina: Historical review and first report of Dirofilaria immitis in a natural mosquito population. Vet Parasitol. 2006; 136 (3-4):259-73.Vieira C, Montoya M, Agudelo S, Velez ID, Simón F. Human antibody response to a 56-kDa purified excretory/secretory product of Dirofilaria immitis. Trop Med Int Health. 2000; 5 (12): 855-859.Vieria C, Vélez ID, Montoya MN, Agudelo S, Alvarez MI, Genchi C, Simón F. Dirofilaria immitis in Tikuna Indians and their dogs in the Colombian Amazon. Ann Trop Med Parasitol. 1998; 92(1): 123-5.Wei L, Patrick K, Ackerson K, El.Mahallawy HS, Kaltenboeck B, Wang C. Molecular detection of Dirofilaria immitis, Hepatozoon canis, Babesia spp., Anaplasma platys and Ehrlichia canis in dogs on Costa Rica. Ata Parasitol. 2014; 60(1): 21-5.Weil GJ, Malane MS, Powers KG. Detection of Circulating Parasite Antigens in Canine Dirofilariasis by Counterimmunoelectrophoresis. Am J Med Hyg. 1984; 33(3): 425-30.Weil GJ, Malane MS, Powers KG, Blair LS. Monoclonal antibodies to parasite antigens found in the serum of Dirofilaria immitis-infected dogs. J. Immunol. 1985; 134: 1185–1191.Zueva T, Morchón R, Carretón E, Ollari-Ib´ñez C, Pericacho M, Rodríguez-Barbero A, Simón F. Angiogenesis in cardiopulmonary dirofilariosis: does teh wolbachia surface protein have a pro- or anti-angiogenbic effect. J Heminthol. 2020; 10(94): 162.DirofilariosisSalud públicaZoonosisDiagnóstico molecularMosquitoHeartwormPublic healthZoonosisMolecular diagnosisMosquitoPublicationLICENSElicense.txtlicense.txttext/plain; charset=utf-815543https://repositorio.unicordoba.edu.co/bitstreams/769cbe78-87d2-48cc-8c9e-5133666412ee/download73a5432e0b76442b22b026844140d683MD51ORIGINALdelavallegalvisrodrigo.pdfdelavallegalvisrodrigo.pdfapplication/pdf2262369https://repositorio.unicordoba.edu.co/bitstreams/131570e9-8281-4fc0-9f9b-845c9490d499/download5f9c4434dd660a83a7c5326cede2e16fMD52Formato de autorizacion.pdfFormato de autorizacion.pdfapplication/pdf376955https://repositorio.unicordoba.edu.co/bitstreams/b70742f7-4d1c-4958-a4e2-fce52bb752c3/downloadeec00a47f48774fe565b839b46e14e5eMD53TEXTdelavallegalvisrodrigo.pdf.txtdelavallegalvisrodrigo.pdf.txtExtracted texttext/plain101427https://repositorio.unicordoba.edu.co/bitstreams/ae7fe46f-ef27-42b6-8298-d33840865ab7/download85b87cef75e81e498edd55db24674c42MD54Formato 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