Análisis químico y evaluación de la actividad biológica de extractos orgánicos de holothuria spp. frente a patógenos intrahospitalarios en Montería, Colombia

La biodiversidad marina ha dado lugar a una extraordinaria variedad de compuestos químicos, muchos de ellos con propiedades biológicas excepcionales. Estos metabolitos secundarios, le confieren ventajas adaptativas a los organismos marinos, las cuales han sido utilizadas por su gran potencial en áre...

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Autores:: Julio Berrio, Mirleth

Tipo de recurso:

Fecha de publicación:: 2025

Institución:: Universidad de Córdoba

Repositorio:: Repositorio Institucional Unicórdoba

Idioma:: spa

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network_acronym_str	UCORDOBA2
network_name_str	Repositorio Institucional Unicórdoba
repository_id_str
dc.title.spa.fl_str_mv	Análisis químico y evaluación de la actividad biológica de extractos orgánicos de holothuria spp. frente a patógenos intrahospitalarios en Montería, Colombia
title	Análisis químico y evaluación de la actividad biológica de extractos orgánicos de holothuria spp. frente a patógenos intrahospitalarios en Montería, Colombia
spellingShingle	Análisis químico y evaluación de la actividad biológica de extractos orgánicos de holothuria spp. frente a patógenos intrahospitalarios en Montería, Colombia Holoturoideos Ácidos grasos Actividad biológica Actividad antioxidante Córdoba Sea cucumbers Fatty acids Biological activity Antioxidant activity Córdoba
title_short	Análisis químico y evaluación de la actividad biológica de extractos orgánicos de holothuria spp. frente a patógenos intrahospitalarios en Montería, Colombia
title_full	Análisis químico y evaluación de la actividad biológica de extractos orgánicos de holothuria spp. frente a patógenos intrahospitalarios en Montería, Colombia
title_fullStr	Análisis químico y evaluación de la actividad biológica de extractos orgánicos de holothuria spp. frente a patógenos intrahospitalarios en Montería, Colombia
title_full_unstemmed	Análisis químico y evaluación de la actividad biológica de extractos orgánicos de holothuria spp. frente a patógenos intrahospitalarios en Montería, Colombia
title_sort	Análisis químico y evaluación de la actividad biológica de extractos orgánicos de holothuria spp. frente a patógenos intrahospitalarios en Montería, Colombia
dc.creator.fl_str_mv	Julio Berrio, Mirleth
dc.contributor.advisor.none.fl_str_mv	Santafé Patiño, Gilmar Quirós-Rodríguez, Jorge A.
dc.contributor.author.none.fl_str_mv	Julio Berrio, Mirleth
dc.contributor.jury.none.fl_str_mv	Oviedo Zumaqué, Luis Eliécer Valle Zapata, Hernan Alonso
dc.subject.proposal.spa.fl_str_mv	Holoturoideos Ácidos grasos Actividad biológica Actividad antioxidante Córdoba
topic	Holoturoideos Ácidos grasos Actividad biológica Actividad antioxidante Córdoba Sea cucumbers Fatty acids Biological activity Antioxidant activity Córdoba
dc.subject.keywords.eng.fl_str_mv	Sea cucumbers Fatty acids Biological activity Antioxidant activity Córdoba
description	La biodiversidad marina ha dado lugar a una extraordinaria variedad de compuestos químicos, muchos de ellos con propiedades biológicas excepcionales. Estos metabolitos secundarios, le confieren ventajas adaptativas a los organismos marinos, las cuales han sido utilizadas por su gran potencial en áreas como la medicina, debido a sus propiedades anticancerígenas, antimicrobianas, antifúngicas y antioxidantes. En este estudio se identificaron estructuralmente 23 compuestos mediante la cromatografía de gases acoplado a espectrometría de masas de impacto electrónico. De los 23 compuestos aislados 22 correspondían a ácidos grasos saturados e insaturados y uno de naturaleza esterólica. Por otro lado, mediante la evaluación de la actividad antioxidante del extracto etanólico de la especie Holothuria grisea frente al radical catiónico ABTS+● se determinó el valor de IC50 de 235,32 μg/mL. En cuanto al ensayo antioxidante por el método del radical DPPH● se determinó un IC50 de 1604,27 μg/mL. La evaluación de la actividad antibacteriana de las dos especies de holotúridos se realizó frente a los aislados clínicos Gram positivas (Staphylococcus aureus, Enterococcus faecium) y Gram negativas (Escherichia coli, Stenotrophomonas maltophilia) por el método de microdilución. El extracto etanólico de H. grisea presentó porcentajes significativos a 2000 mg/L reduciendo el crecimiento de estos microorganismos, destacándose la mayor sensibilidad en la especie S. maltophilia con un porcentaje de reducción de crecimiento del 100% a 1250 mg/L, seguido de E. coli y E. faecium con porcentajes de reducción de 99,34% y 91,85% respectivamente. Por su parte, el extracto etanólico de H. princeps, no presentó una considerable inhibición del crecimiento en estas cepas bacterianas. Para la evaluación de la actividad antifúngica frente a las cepas del género Candida (C. albicans, C. tropicalis, C. glabrata y C. auris), a nivel general los mejores resultados de inhibición de crecimiento se mostraron a mayores concentraciones de los extractos etanólicos (desde 2500 mg/L). Se evidenció que los aislados clínicos C. tropicalis y C. glabrata, fueron las cepas fúngicas más susceptible al extracto etanólico de H. grisea, presentando una reducción de crecimiento del 100% a 2500 mg/L y para H. princeps la cepa fúngica más suceptible fue C. glabrata, la cual presentó una reducción de crecimiento de 95,7% a 5000 mg/L.
publishDate	2025
dc.date.accessioned.none.fl_str_mv	2025-02-05T17:13:39Z
dc.date.available.none.fl_str_mv	2025-02-05T17:13:39Z 2026-12-31
dc.date.issued.none.fl_str_mv	2025-02-03
dc.type.none.fl_str_mv	Trabajo de grado - Maestría
dc.type.driver.none.fl_str_mv	info:eu-repo/semantics/masterThesis
dc.type.version.none.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.none.fl_str_mv	Text
dc.type.redcol.none.fl_str_mv	http://purl.org/redcol/resource_type/TM
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unicordoba.edu.co/handle/ucordoba/9007
dc.identifier.instname.none.fl_str_mv	Universidad de Córdoba
dc.identifier.reponame.none.fl_str_mv	Repositorio Universidad de Córdoba
dc.identifier.repourl.none.fl_str_mv	https://repositorio.unicordoba.edu.co/
url	https://repositorio.unicordoba.edu.co/handle/ucordoba/9007 https://repositorio.unicordoba.edu.co/
identifier_str_mv	Universidad de Córdoba Repositorio Universidad de Córdoba
dc.language.iso.none.fl_str_mv	spa
language	spa
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Wang, F., Cao, Y., Guo, Y., Zhu, Z., & Zhang, C. (2021). Evaluation of antioxidant and antibacterial activities of lipid extracts from Eustigmatos cf. polyphem (Eustigmatophyceae) and preliminary identification of bioactive compound. Algal Research, 59. https://doi.org/10.1016/j.algal.2021.102446 Wargasetia, T. L., Ratnawati, H., Widodo, N., & Widyananda, M. H. (2023). Antioxidant and anti-inflammatory activity of sea cucumber (Holothuria scabra) active compounds against KEAP1 and iNOS protein. Bioinformatics and Biology Insights, 17, 11779322221149613. Yuan, H., Ma, Q., Ye, L., & Piao, G. (2016). The Traditional Medicine and Modern Medicine from Natural Products. Molecules, 21(5), 559. https://doi.org/10.3390/molecules21050559 Zhao, Y., Wang, Y., Zhang, X., Liu, H., Wang, G., & Liu, H. (2012). Effects of potential probiotic Bacillus subtilis T13 on growth, immunity and disease resistance against Vibrio splendidus infection in juvenile sea cucumber Apostichopus japonicus. 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spelling	Santafé Patiño, Gilmar80ca173d-544e-4b89-adc4-c08cc8c350b7600Quirós-Rodríguez, Jorge A.ebba5734-522e-4d6b-b400-3cc24b8bb851600Julio Berrio, Mirlethcd33245d-aa0a-403c-abe7-dcd4dc077729600Oviedo Zumaqué, Luis Eliécer16244141-aa15-45d4-a620-56014ced68f7600Valle Zapata, Hernan Alonso744348e2-deca-4ed4-9b02-05a6766f4f346002025-02-05T17:13:39Z2026-12-312025-02-05T17:13:39Z2025-02-03https://repositorio.unicordoba.edu.co/handle/ucordoba/9007Universidad de CórdobaRepositorio Universidad de Córdobahttps://repositorio.unicordoba.edu.co/La biodiversidad marina ha dado lugar a una extraordinaria variedad de compuestos químicos, muchos de ellos con propiedades biológicas excepcionales. Estos metabolitos secundarios, le confieren ventajas adaptativas a los organismos marinos, las cuales han sido utilizadas por su gran potencial en áreas como la medicina, debido a sus propiedades anticancerígenas, antimicrobianas, antifúngicas y antioxidantes. En este estudio se identificaron estructuralmente 23 compuestos mediante la cromatografía de gases acoplado a espectrometría de masas de impacto electrónico. De los 23 compuestos aislados 22 correspondían a ácidos grasos saturados e insaturados y uno de naturaleza esterólica. Por otro lado, mediante la evaluación de la actividad antioxidante del extracto etanólico de la especie Holothuria grisea frente al radical catiónico ABTS+● se determinó el valor de IC50 de 235,32 μg/mL. En cuanto al ensayo antioxidante por el método del radical DPPH● se determinó un IC50 de 1604,27 μg/mL. La evaluación de la actividad antibacteriana de las dos especies de holotúridos se realizó frente a los aislados clínicos Gram positivas (Staphylococcus aureus, Enterococcus faecium) y Gram negativas (Escherichia coli, Stenotrophomonas maltophilia) por el método de microdilución. El extracto etanólico de H. grisea presentó porcentajes significativos a 2000 mg/L reduciendo el crecimiento de estos microorganismos, destacándose la mayor sensibilidad en la especie S. maltophilia con un porcentaje de reducción de crecimiento del 100% a 1250 mg/L, seguido de E. coli y E. faecium con porcentajes de reducción de 99,34% y 91,85% respectivamente. Por su parte, el extracto etanólico de H. princeps, no presentó una considerable inhibición del crecimiento en estas cepas bacterianas. Para la evaluación de la actividad antifúngica frente a las cepas del género Candida (C. albicans, C. tropicalis, C. glabrata y C. auris), a nivel general los mejores resultados de inhibición de crecimiento se mostraron a mayores concentraciones de los extractos etanólicos (desde 2500 mg/L). Se evidenció que los aislados clínicos C. tropicalis y C. glabrata, fueron las cepas fúngicas más susceptible al extracto etanólico de H. grisea, presentando una reducción de crecimiento del 100% a 2500 mg/L y para H. princeps la cepa fúngica más suceptible fue C. glabrata, la cual presentó una reducción de crecimiento de 95,7% a 5000 mg/L.Marine biodiversity has led to an extraordinary variety of chemical compounds, many of them with exceptional biological properties. These secondary metabolites confer adaptive advantages to marine organisms, which have been used for their great potential in areas such as medicine, due to their anticancer, antimicrobial, antifungal and antioxidant properties. In this study, 23 compounds were structurally identified by gas chromatography coupled to electron impact mass spectrometry. Of the 23 compounds isolated, 22 corresponded to saturated and unsaturated fatty acids and one of a sterol nature. On the other hand, by evaluating the antioxidant activity of the ethanolic extract of the species Holothuria grisea against the cationic radical ABTS+●, the IC50 value of 235.32 μg/mL was determined. Regarding the antioxidant assay by the DPPH● radical method, an IC50 of 1604.27 μg/mL was determined. The evaluation of the antibacterial activity of the two species of sea cucumbers was carried out against the Gram-positive (Staphylococcus aureus, Enterococcus faecium) and Gram-negative (Escherichia coli, Stenotrophomonas maltophilia) clinical isolates by the microdilution method. The ethanolic extract of H. grisea presented significant percentages at 2000 mg/L, reducing the growth of these microorganisms, with the greatest sensitivity being highlighted in the S. maltophilia species with a growth reduction percentage of 100% at 1250 mg/L, followed by E. coli and E. faecium with reduction percentages of 99.34% and 91.85%, respectively. On the other hand, the ethanolic extract of H. princeps did not present a considerable inhibition of growth in these bacterial strains. For the evaluation of antifungal activity against strains of the genus Candida (C. albicans, C. tropicalis, C. glabrata and C. auris), in general the best results of growth inhibition were shown Marine biodiversity has led to an extraordinary variety of chemical compounds, many of them with exceptional biological properties. These secondary metabolites confer adaptive advantages to marine organisms, which have been used for their great potential in areas such as medicine, due to their anticancer, antimicrobial, antifungal and antioxidant properties. In this study, 23 compounds were structurally identified by gas chromatography coupled to electron impact mass spectrometry. Of the 23 compounds isolated, 22 corresponded to saturated and unsaturated fatty acids and one of a sterol nature. On the other hand, by evaluating the antioxidant activity of the ethanolic extract of the species Holothuria grisea against the cationic radical ABTS+●, the IC50 value of 235.32 μg/mL was determined. Regarding the antioxidant assay by the DPPH● radical method, an IC50 of 1604.27 μg/mL was determined. The evaluation of the antibacterial activity of the two species of sea cucumbers was carried out against the Gram-positive (Staphylococcus aureus, Enterococcus faecium) and Gram-negative (Escherichia coli, Stenotrophomonas maltophilia) clinical isolates by the microdilution method. The ethanolic extract of H. grisea presented significant percentages at 2000 mg/L, reducing the growth of these microorganisms, with the greatest sensitivity being highlighted in the S. maltophilia species with a growth reduction percentage of 100% at 1250 mg/L, followed by E. coli and E. faecium with reduction percentages of 99.34% and 91.85%, respectively. On the other hand, the ethanolic extract of H. princeps did not present a considerable inhibition of growth in these bacterial strains. For the evaluation of antifungal activity against strains of the genus Candida (C. albicans, C. tropicalis, C. glabrata and C. auris), in general the best results of growth inhibition were shown Marine biodiversity has led to an extraordinary variety of chemical compounds, many of them with exceptional biological properties. These secondary metabolites confer adaptive advantages to marine organisms, which have been used for their great potential in areas such as medicine, due to their anticancer, antimicrobial, antifungal and antioxidant properties. In this study, 23 compounds were structurally identified by gas chromatography coupled to electron impact mass spectrometry. Of the 23 compounds isolated, 22 corresponded to saturated and unsaturated fatty acids and one of a sterol nature. On the other hand, by evaluating the antioxidant activity of the ethanolic extract of the species Holothuria grisea against the cationic radical ABTS+●, the IC50 value of 235.32 μg/mL was determined. Regarding the antioxidant assay by the DPPH● radical method, an IC50 of 1604.27 μg/mL was determined. The evaluation of the antibacterial activity of the two species of sea cucumbers was carried out against the Gram-positive (Staphylococcus aureus, Enterococcus faecium) and Gram-negative (Escherichia coli, Stenotrophomonas maltophilia) clinical isolates by the microdilution method. The ethanolic extract of H. grisea presented significant percentages at 2000 mg/L, reducing the growth of these microorganisms, with the greatest sensitivity being highlighted in the S. maltophilia species with a growth reduction percentage of 100% at 1250 mg/L, followed by E. coli and E. faecium with reduction percentages of 99.34% and 91.85%, respectively. On the other hand, the ethanolic extract of H. princeps did not present a considerable inhibition of growth in these bacterial strains. For the evaluation of antifungal activity against strains of the genus Candida (C. albicans, C. tropicalis, C. glabrata and C. auris), in general the best results of growth inhibition were shown at higher concentrations of the ethanolic extracts (from 2500 mg/L). It was evidenced that the clinical isolates C. tropicalis and C. glabrata were the most susceptible fungal strains to the ethanolic extract of H. grisea, presenting a 100% reduction in growth at 2500 mg/L, and for H. princeps the most susceptible fungal strain was C. glabrata, which presented a 95.7% reduction in growth at 5000 mg/L.INTRODUCCIÓN ..............................................151. OBJETIVOS ...................................................191.1 OBJETIVO GENERAL ...........................................191.2 OBJETIVOS ESPECÍFICOS ................................192. MARCO TEÓRICO ............................................202.1 MARCO REFERENCIAL ........................................212.2 PHYLUM ECHINODERMATA ...............242.3 CLASE Holothuroidea ............................252.3.1 Descripción general y taxonomía de la especie de Holothuria grisea. .........272.3.2 Descripción general y taxonomía de la especie de Holothuria princeps. .........282.4 ENFERMEDADES CAUSADAS POR MICROORGANISMOS Y EFECTOS DEL PROCESO DE OXIDACIÓN ..........302.4.1 Estrés oxidativo ...................................302.4.2 Enfermedades causadas por microorganismos ..............................312.4.3 Infecciones Asociadas a la Atención en Salud (IAAS) de microorganismos patógenos .......................... 323. MATERIALES Y MÉTODOS ...............343.1 RECOLECCIÓN DEL MATERIAL BIOLÓGICO ..............343.2 PREPARACIÓN DEL MATERIAL BIOLÓGICO ................343.3 ESTUDIO QUÍMICO DE H. grisea MEDIANTE EL USO DE TÉCNICAS CROMATOGRÁFICAS COMO CROMATOGRAFÍA EN COLUMNA (CC), CROMATOGRAFÍA EN CAPA DELGADA (CCD) y CGAR-EM ......353.3.1 FRACCIONAMIENTO CROMATOGRÁFICO DE H. grisea ........................353.3.2 DERIVATIZACIÓN DE ÁCIDOS GRASOS ...........................................353.3.3 IDENTIFICACIÓN QUÍMICA DE LOS METABOLITOS SECUNDARIOS MAYORITARIOS AISLADOS DE H. grisea, MEDIANTE CGAR-EM .............363.4 EVALUACIÓN DE LA ACTIVIDAD ANTIOXIDANTE FRENTE A LOS RADICALES ABTS+•, DPPH• DEL EXTRACTO ETANÓLICO DE LA ESPECIE H. grisea ...............363.4.1 Protocolo del ensayo ABTS+• ............................373.4.2 Protocolo del ensayo DPPH• ..............................393.5 ACTIVIDAD ANTIBACTERIANA Y ANTIFÚNGICA DE LOS EXTRACTOS ETANÓLICOS DE LAS ESPECIES Holothuria princeps Y Holothuria grisea SOBRE LOS AISLADOS CLÍNICOS INTRAHOSPITALARIOS .....403.5.1 Evaluación de la actividad antibacteriana ............403.5.1.1 Pruebas de susceptibilidad antibacteriana ..................413.5.2 Evaluación de la actividad antifúngica ............................433.5.2.1 Pruebas de susceptibilidad antifúngica ..............................433.6 ANÁLISIS ESTADÍSTICO ...............................444. RESULTADOS Y ANÁLISIS DE RESULTADOS .......................464.1 IDENTIFICACIÓN QUÍMICA DE LOS METABOLITOS SECUNDARIOS MAYORITARIOS AISLADOS DE H. grisea, MEDIANTE CGAR-EM. ................................................................464.1.1 Compuestos aislados de H. grisea ................................464.1.1.1 Compuesto HG4 de H. grisea ...........................484.1.1.2 Compuesto HG11 de H. grisea .........................504.1.1.3 Compuesto HG14 de H. grisea .............................524.1.1.4 Compuesto HG19 de H. grisea .................................554.2 EVALUACIÓN DE LA ACTIVIDAD ANTIOXIDANTE FRENTE A LOS RADICALES ABTS+• y DPPH• DEL EXTRACTO ETANÓLICO DE LA ESPECIE H. grisea. ..........644.2.1 Evaluación de la actividad antioxidante frente al radical ABTS+• del extracto etanólico de la especie H. grisea. ...................................654.2.2 Evaluación de la actividad antioxidante frente al radical DPPH• del extracto etanólico de la especie H. grisea. ...................................674.3 EVALUACIÓN DE LA ACTIVIDAD ANTIMICROBIANA DE LOS EXTRACTOS ETANÓLICOS DE LAS ESPECIES Holothuria princeps y Holothuria grisea SOBRE LOS AISLADOS CLÍNICOS INTRAHOSPITALARIOS. .................................................................724.3.1 Ensayo de la actividad antibacteriana de las especies Holothuria princeps y Holothuria grisea sobre los aislados clínicos intrahospitalarios .............................724.3.2 Ensayo de la actividad antifúngica de las especies Holothuria grisea y Holothuria princeps sobre los aislados clínicos intrahospitalarios ...................795. CONCLUSIONES ............................................876. BIBLIOGRAFIA ......................................897. ANEXOS ......................................................... 102MaestríaMagíster en Ciencias QuímicasTrabajos de Investigación y/o Extensiónapplication/pdfspaUniversidad de CórdobaFacultad de Ciencias BásicasMontería, Córdoba, ColombiaMaestría en Ciencias QuímicasCopyright Universidad de Córdoba, 2025https://creativecommons.org/licenses/by-nc-nd/4.0/Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)info:eu-repo/semantics/embargoedAccesshttp://purl.org/coar/access_right/c_f1cfAnálisis químico y evaluación de la actividad biológica de extractos orgánicos de holothuria spp. frente a patógenos intrahospitalarios en Montería, ColombiaTrabajo de grado - Maestríainfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/acceptedVersionTexthttp://purl.org/redcol/resource_type/TMAhmed, H. O., Mahdy, A., Nasser, S. A. M., El-Wakeil, K. F. 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

Análisis químico y evaluación de la actividad biológica de extractos orgánicos de holothuria spp. frente a patógenos intrahospitalarios en Montería, Colombia

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