Caracterización de bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco arenosos, como alternativa de biofertilización

Los microorganismos del suelo son fundamentales para el equilibrio ecológico, debido a que participan activamente en los ciclos de elementos esenciales como el carbono, nitrógeno, azufre y fósforo. Entre estos, las bacterias solubilizadoras de fósforo y productoras de ácido indol acético desempeñan...

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Autores:: Mercado Rosso, Sara Yomar

Tipo de recurso:: Trabajo de grado de pregrado

Fecha de publicación:: 2024

Institución:: Universidad de Córdoba

Repositorio:: Repositorio Institucional Unicórdoba

Idioma:: spa

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network_acronym_str	UCORDOBA2
network_name_str	Repositorio Institucional Unicórdoba
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dc.title.spa.fl_str_mv	Caracterización de bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco arenosos, como alternativa de biofertilización
title	Caracterización de bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco arenosos, como alternativa de biofertilización
spellingShingle	Caracterización de bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco arenosos, como alternativa de biofertilización Biofertilizantes Bacterias promotoras del crecimiento vegetal Departamento de Córdoba Biofertilizers plant growth promoting bacteria Department of Córdoba
title_short	Caracterización de bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco arenosos, como alternativa de biofertilización
title_full	Caracterización de bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco arenosos, como alternativa de biofertilización
title_fullStr	Caracterización de bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco arenosos, como alternativa de biofertilización
title_full_unstemmed	Caracterización de bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco arenosos, como alternativa de biofertilización
title_sort	Caracterización de bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco arenosos, como alternativa de biofertilización
dc.creator.fl_str_mv	Mercado Rosso, Sara Yomar
dc.contributor.advisor.none.fl_str_mv	Cantero Guevara, Miriam Elena Betin Ruiz , Andrés José
dc.contributor.author.none.fl_str_mv	Mercado Rosso, Sara Yomar
dc.contributor.jury.none.fl_str_mv	Oviedo Zumaqué, Luis Eliécer Villalba Anaya, Mara de la Concepción
dc.subject.proposal.none.fl_str_mv	Biofertilizantes Bacterias promotoras del crecimiento vegetal Departamento de Córdoba
topic	Biofertilizantes Bacterias promotoras del crecimiento vegetal Departamento de Córdoba Biofertilizers plant growth promoting bacteria Department of Córdoba
dc.subject.keywords.none.fl_str_mv	Biofertilizers plant growth promoting bacteria Department of Córdoba
description	Los microorganismos del suelo son fundamentales para el equilibrio ecológico, debido a que participan activamente en los ciclos de elementos esenciales como el carbono, nitrógeno, azufre y fósforo. Entre estos, las bacterias solubilizadoras de fósforo y productoras de ácido indol acético desempeñan un papel crucial en la rizosfera. Estas bacterias han atraído un gran interés en el campo de la agricultura por su potencial uso como biofertilizantes para mejorar la nutrición de los cultivos. El objetivo de esta investigación fue caracterizar bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco-arenoso como alternativa de biofertilización. A las cepas aisladas se les realizó la prueba de solubilización de fósforo empleando el Método de Molibdovanadato y la producción de ácido indol acético se evaluó a través del reactivo de Salkowski. Las cepas fueron identificadas molecularmente utilizando los cebadores universales 27F, 5′-AGAGTTTGATCMTGGCTCAG-3′ y 1492R, 5′-TACGGYTACCTTGTTACGACTT-3′, que amplifican el gen 16S rRNA. Se realizó un análisis de varianza unidireccional (ANOVA) siguiendo la prueba post hoc de Tukey (p <0.05), con un nivel de significancia del 5%. La secuenciación del gen 16S rRNA revela la confirmación de aislamientos de Enterobacter cloacae (cepa S105E PP405613.1 y S106F PP2688674632) y Enterobacter hormaechei (cepa S104B PP 2688674632. En condiciones in vitro, se encontró que la cepa S105E solubilizó una concentración de fósforo de 2224.73 ± 26.16 mg L-1, seguida por S104B con 2169.11 ± 49.31 mg L-1 y S106F con 2028.62 ± 44.03 mg L-1, por otro lado, las cepas 11 y 19 produjeron una concentración de ácido indol acético de 27.60 ± 0.25 mg L-1. Las cepas nativas S105E, S106F, S104B, 11 y 19 demostraron gran capacidad de solubilización de fósforo y producción de ácido indol acético en condiciones in vitro.
publishDate	2024
dc.date.accessioned.none.fl_str_mv	2024-11-16T16:34:05Z
dc.date.available.none.fl_str_mv	2024-11-16T16:34:05Z 2025-11-14
dc.date.issued.none.fl_str_mv	2024-11-15
dc.type.none.fl_str_mv	Trabajo de grado - Pregrado
dc.type.driver.none.fl_str_mv	info:eu-repo/semantics/bachelorThesis
dc.type.coar.none.fl_str_mv	http://purl.org/coar/resource_type/c_7a1f
dc.type.version.none.fl_str_mv	info:eu-repo/semantics/acceptedVersion
dc.type.content.none.fl_str_mv	Text
format	http://purl.org/coar/resource_type/c_7a1f
status_str	acceptedVersion
dc.identifier.uri.none.fl_str_mv	https://repositorio.unicordoba.edu.co/handle/ucordoba/8746
dc.identifier.instname.none.fl_str_mv	Universidad de Córdoba
dc.identifier.reponame.none.fl_str_mv	Repositorio Institucional Unicórdoba
dc.identifier.repourl.none.fl_str_mv	https://repositorio.unicordoba.edu.co
url	https://repositorio.unicordoba.edu.co/handle/ucordoba/8746 https://repositorio.unicordoba.edu.co
identifier_str_mv	Universidad de Córdoba Repositorio Institucional Unicórdoba
dc.language.iso.none.fl_str_mv	spa
language	spa
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spelling	Cantero Guevara, Miriam Elena3e8eda8e-66c5-4436-b6e6-bec1bc12b57f-1Betin Ruiz , Andrés José7f3427db-1e75-48be-ba50-571dc44cba38600Mercado Rosso, Sara Yomar0be5e25d-f2fc-462c-9466-7cbfd6300477-1Oviedo Zumaqué, Luis Eliécer16244141-aa15-45d4-a620-56014ced68f7-1Villalba Anaya, Mara de la Concepciónfedd63bd-a245-463e-8b72-9f6cfd07234d-12024-11-16T16:34:05Z2025-11-142024-11-16T16:34:05Z2024-11-15https://repositorio.unicordoba.edu.co/handle/ucordoba/8746Universidad de CórdobaRepositorio Institucional Unicórdobahttps://repositorio.unicordoba.edu.coLos microorganismos del suelo son fundamentales para el equilibrio ecológico, debido a que participan activamente en los ciclos de elementos esenciales como el carbono, nitrógeno, azufre y fósforo. Entre estos, las bacterias solubilizadoras de fósforo y productoras de ácido indol acético desempeñan un papel crucial en la rizosfera. Estas bacterias han atraído un gran interés en el campo de la agricultura por su potencial uso como biofertilizantes para mejorar la nutrición de los cultivos. El objetivo de esta investigación fue caracterizar bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco-arenoso como alternativa de biofertilización. A las cepas aisladas se les realizó la prueba de solubilización de fósforo empleando el Método de Molibdovanadato y la producción de ácido indol acético se evaluó a través del reactivo de Salkowski. Las cepas fueron identificadas molecularmente utilizando los cebadores universales 27F, 5′-AGAGTTTGATCMTGGCTCAG-3′ y 1492R, 5′-TACGGYTACCTTGTTACGACTT-3′, que amplifican el gen 16S rRNA. Se realizó un análisis de varianza unidireccional (ANOVA) siguiendo la prueba post hoc de Tukey (p <0.05), con un nivel de significancia del 5%. La secuenciación del gen 16S rRNA revela la confirmación de aislamientos de Enterobacter cloacae (cepa S105E PP405613.1 y S106F PP2688674632) y Enterobacter hormaechei (cepa S104B PP 2688674632. En condiciones in vitro, se encontró que la cepa S105E solubilizó una concentración de fósforo de 2224.73 ± 26.16 mg L-1, seguida por S104B con 2169.11 ± 49.31 mg L-1 y S106F con 2028.62 ± 44.03 mg L-1, por otro lado, las cepas 11 y 19 produjeron una concentración de ácido indol acético de 27.60 ± 0.25 mg L-1. Las cepas nativas S105E, S106F, S104B, 11 y 19 demostraron gran capacidad de solubilización de fósforo y producción de ácido indol acético en condiciones in vitro.Soil microorganisms are essential for ecological balance, since they actively participate in the cycles of essential elements such as carbon, nitrogen, sulfur and phosphorus. Among these, phosphorus-solubilizing and indole-acetic acid-producing bacteria play a crucial role in the rhizosphere. These bacteria have attracted great interest in the field of agriculture for their potential use as biofertilizers to improve crop nutrition. The objective of this research was to characterize native phosphorus-solubilizing and indole-acetic acid-producing bacteria isolated from sandy loam soil as an alternative for biofertilization. The isolated strains were tested for phosphorus solubilization using the Molybdovanadate Method and the production of indole-acetic acid was evaluated through the Salkowski reagent. Strains were molecularly identified using universal primers 27F, 5′-AGAGTTTGATCMTGGCTCAG-3′ and 1492R, 5′-TACGGYTACCTTGTTACGACTT-3′, which amplify the 16S rRNA gene. A one-way analysis of variance (ANOVA) was performed following Tukey's post hoc test (p < 0.05), with a significance level of 5%. 16S rRNA gene sequencing reveals confirmation of Enterobacter cloacae (strain S105E PP405613.1 and S106F PP2688674632) and Enterobacter hormaechei (strain S104B PP 2688674632) isolates. Under in vitro conditions, strain S105E was found to solubilise a phosphorus concentration of 2224.73 ± 26.16 mg L-1, followed by S104B with 2169.11 ± 49.31 mg L-1 and S106F with 2028.62 ± 44.03 mg L-1, on the other hand, strains 11 and 19 produced an indole acetic acid concentration of 27.60 ± 0.25 mg L-1. Native strains S105E, S106F, S104B, 11 and 19 showed great capacity for phosphorus solubilization and indole acetic acid production under in vitro conditions.Resumen....................................................................111. Introducción ................................................... 132. Objetivos...................................................... 152.1. Objetivo General ..........................................152.2. Objetivos Específicos.................................153. Antecedentes................................................. 164. Marco Teórico............................................ 184.1. Formas Básicas y Ciclos del Fósforo en el Suelo ....................... 184.1.1. Fosforo Inorgánico en el Suelo ....................................194.2. Ácido Indol Acético (AIA)........................................ 204.2. Bacterias Promotoras del Crecimiento Vegetal (PGPB) .......................214.2.1. Bacterias Solubilizadoras de Fósforo ........................224.2.1.1. Biodiversidad de Bacterias Solubilizadoras de Fósforo ....................224.2.2.2. Mecanismo de Solubilización de Fósforo................... 235. Metodología ...................................................... 275.1. Tipo de Estudio ..............................................275.2. Área de Estudio ........................................275.3. Identificación de Bacterias Nativas Solubilizadoras de Fósforo y Productoras de Ácido Indol Acético Aisladas a Partir de Muestras de Suelo Franco-Arenoso...........275.3.1. Muestreo de Suelo...................................... 275.3.2. Aislamiento de Bacterias Nativas Solubilizadoras de Fósforo y Productoras de Ácido Indol Acético ............................275.3.3. Identificación Molecular de BSF y Productoras de AIA........................................285.3.3.1. Extracción de ADN Genómico..................................285.3.3.2. Amplificación de ADN Mediante Reacción en Cadena de la Polimerasa (PCR) 285.3.3.3. Secuenciación de Productos de PCR y Análisis de Secuencia............................ 295.4. Evaluación in vitro de la Capacidad Solubilizadora de Fosforo y Productora de AIA de las Cepas Aisladas .................... 295.4.1. Detección in vitro de la Actividad Solubilizadora de Fósforo................................ 295.4.2. Cuantificación in vitro de la Producción de AIA................ 306. Resultados y Discusión...........................................316.1. Aislamiento de BSF y Productora de AIA ..................316.2. Evaluación in vitro de la Actividad Solubilizadora de Fósforo y Productora de AIA....326.2.1. Detección in vitro de la Actividad Solubilizadora de Fósforo................................ 326.2.2. Cuantificación in vitro de la Producción de AIA..................... 366.3. Identificación Molecular de BSF y Productoras de AIA...............................................377. Conclusiones......................................... 398. Recomendaciones..............................................409. Referencias Bibliográficas................................4110. Anexos ............................................. 53PregradoQuímico(a)Trabajos de Investigación y/o Extensiónapplication/pdfspaUniversidad de CórdobaFacultad de Ciencias BásicasMontería, Córdoba, ColombiaQuímicaCopyright Universidad de Córdoba, 2024https://creativecommons.org/licenses/by-nc/4.0/Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)info:eu-repo/semantics/embargoedAccesshttp://purl.org/coar/access_right/c_f1cfCaracterización de bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco arenosos, como alternativa de biofertilizaciónTrabajo de grado - Pregradoinfo:eu-repo/semantics/bachelorThesishttp://purl.org/coar/resource_type/c_7a1finfo:eu-repo/semantics/acceptedVersionTextAlori, E. T., Glick, B. R., & Babalola, O. O. (2017). Microbial Phosphorus Solubilization and Its Potential for Use in Sustainable Agriculture. Frontiers in Microbiology, 8, 971. 42 https://doi.org/10.3389/fmicb.2017.00971Asea, P. 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Science of The Total Environment, 612, 522- 537. https://doi.org/10.1016/j.scitotenv.2017.08.095BiofertilizantesBacterias promotoras del crecimiento vegetalDepartamento de CórdobaBiofertilizersplant growth promoting bacteriaDepartment of CórdobaPublicationORIGINALINFORME FINAL DE TRABAJO DE GRADO- SARA YOMAR MERCADO ROSSO (1).pdfINFORME FINAL DE TRABAJO DE GRADO- SARA YOMAR MERCADO ROSSO (1).pdfapplication/pdf644261https://repositorio.unicordoba.edu.co/bitstreams/83275963-ab68-42b1-9e04-53b5750f0e01/download84c525b710ecc9915f5e13643fb3ab72MD51SARA MERCADO .pdfSARA MERCADO .pdfapplication/pdf773290https://repositorio.unicordoba.edu.co/bitstreams/cac21c8f-f877-4c50-a2a4-a13c52b3ad87/download7f461267e7c9ad4cc24c5bf4f3f6f43eMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-815543https://repositorio.unicordoba.edu.co/bitstreams/46c0b2a8-c7d5-483e-a7f9-9866693dc072/download73a5432e0b76442b22b026844140d683MD53TEXTINFORME FINAL DE TRABAJO DE GRADO- SARA YOMAR MERCADO ROSSO 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

Caracterización de bacterias nativas solubilizadoras de fósforo y productoras de ácido indol acético aisladas de suelo franco arenosos, como alternativa de biofertilización

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