Evaluación de los factores bióticos y abióticos en una cría de planarias
Las planarias, también llamados gusanos planos, son organismos triploblásticos, acoelomatosos, pertenecientes al filo de platelmintos, los cuales se han estudiado ampliamente en áreas de medicina regenerativa y la biología del desarrollo a causa de su gran capacidad de regeneración de tejido a parti...
- Autores:
-
Gonzalez Fontecha, Jossie Esteban
Patiño Herrera, Ariana Yineth
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2022
- Institución:
- Colegio Mayor de Cundinamarca
- Repositorio:
- Repositorio Colegio Mayor de Cundinamarca
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.unicolmayor.edu.co:unicolmayor/5708
- Acceso en línea:
- https://repositorio.unicolmayor.edu.co/handle/unicolmayor/5708
- Palabra clave:
- Planarias
Microbiota
Factores bióticos
Factores abióticos
- Rights
- closedAccess
- License
- Derechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2022
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Evaluación de los factores bióticos y abióticos en una cría de planarias |
| title |
Evaluación de los factores bióticos y abióticos en una cría de planarias |
| spellingShingle |
Evaluación de los factores bióticos y abióticos en una cría de planarias Planarias Microbiota Factores bióticos Factores abióticos |
| title_short |
Evaluación de los factores bióticos y abióticos en una cría de planarias |
| title_full |
Evaluación de los factores bióticos y abióticos en una cría de planarias |
| title_fullStr |
Evaluación de los factores bióticos y abióticos en una cría de planarias |
| title_full_unstemmed |
Evaluación de los factores bióticos y abióticos en una cría de planarias |
| title_sort |
Evaluación de los factores bióticos y abióticos en una cría de planarias |
| dc.creator.fl_str_mv |
Gonzalez Fontecha, Jossie Esteban Patiño Herrera, Ariana Yineth |
| dc.contributor.advisor.none.fl_str_mv |
Sánchez Leal, Ligia Consuelo |
| dc.contributor.author.none.fl_str_mv |
Gonzalez Fontecha, Jossie Esteban Patiño Herrera, Ariana Yineth |
| dc.subject.proposal.spa.fl_str_mv |
Planarias Microbiota Factores bióticos Factores abióticos |
| topic |
Planarias Microbiota Factores bióticos Factores abióticos |
| description |
Las planarias, también llamados gusanos planos, son organismos triploblásticos, acoelomatosos, pertenecientes al filo de platelmintos, los cuales se han estudiado ampliamente en áreas de medicina regenerativa y la biología del desarrollo a causa de su gran capacidad de regeneración de tejido a partir de sus células madre llamadas neoblastos. A pesar de ser un modelo biológico con gran bioprospección no se han estudiado en conjunto los factores bióticos y abióticos que intervienen en su biología y comportamiento. En el presente proyecto se estudiaron los factores bióticos y abióticos que pueden tener algún efecto en el establecimiento y el mantenimiento de una cría de planarias. Se caracterizó la microbiota externa de las planarias, la población bacteriana las piedras, la materia orgánica de la que se alimentan las planarias y el agua de la quebrada La Vieja y se verificó la vitalidad y el comportamiento de las planarias en el establecimiento de su cría en el laboratorio. Se evidenció que las planarias son metazoos con gran capacidad de adaptación a diferentes factores bióticos y abióticos, tolerando cambios de temperatura, conductividad y TDS. Por otra parte, son organismos que albergan una amplia variedad microbiana, y conviven con una gran diversidad de bacterias en su hábitat natural. De esta manera se pudo determinar que son organismos viables para el cultivo en un ambiente controlado de laboratorio, condiciones adecuadas de alimentación, temperatura y otras características que indican la continuidad de una población estable en el tiempo, y de hábitos y comportamiento definidos |
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2022 |
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2022-10-18T13:49:58Z |
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2022-10-18T13:49:58Z |
| dc.date.issued.none.fl_str_mv |
2022-04 |
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Trabajo de grado - Pregrado |
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http://purl.org/coar/resource_type/c_7a1f |
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Text |
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Ivanovic Z, Vlaski-Lafarge M. Metabolic and Genetic Features of Ancestral Eukaryotes versus Metabolism and “Master Pluripotency Genes” of Stem Cells. Anaerobiosis and Stemness [Internet]. 2016 [cited 24 Mar 2021 ];:211-234. Available from: doi: 10.1016/B978-0-12-800540-8.00011-9 https://www.sciencedirect.com/science/article/pii/B9780128005408000119 Cabej NR. Cambrian explosion. In: Cabej NR, editor. Epigenetic Mechanisms of the Cambrian Explosion [Internet]. 2020. [cited 24Mar 2021] p. 137–211. Available from: https://www.sciencedirect.com/science/article/pii/B9780128143117000044 Foster JA. Induction of Neoplasms in Planarians with Carcinogens. Cancer Res. [Internet]. 1963 [cited 10 oct 2020]; 23:300–3. Available in: https://cancerres.aacrjournals.org/content/23/2_Part_1/300.long Schaeffer DJ. Planarians as a model system for in vivo tumorigenesis studies. Ecotoxicol Environ Saf. [Internet]. 1993 [cited 24 Mar 2021]; 25(1):1-18. Available in:https://www.sciencedirect.com/science/article/abs/pii/S0147651383710018?via%3 Dihub doi:10.1006/eesa.1993.1001 Arnold CP, Merryman MS, Harris AA, McKinney S, Seidel C, Loethen S et al. Pathogenic shifts in endogenous microbiota impede tissue regeneration via distinct activation of TAK1/MKK/p38. eLife [Internet]. 2016 [cited 24 Mar 2021];5. Available from: https://pubmed.ncbi.nlm.nih.gov/27441386/ Rompolas P, Patel-King RS, King SM. Schmidtea mediterranea: a model system for analysis of motile cilia. In: Methods in cell biology [Internet]. First edit. Elsevier; 2009 [cited 24 Mar 2021]. p. 81–98. Available from: http://dx.doi.org/10.1016/S0091- 679X(08)93004-1 . Legner EF, Tsai TC, Medved RA. Environmental stimulants to asexual reproduction in the planarian,Dugesia dorotocephala. Biol Control [Internet]. 1976;[cited 27 Mar 2021]21(4):415–23. Available from: https://link.springer.com/article/10.1007/BF02371640 . Lee F, Williams K, Levin M, Wolfe B. The Bacterial Metabolite Indole Inhibits Regeneration of the Planarian Flatworm Dugesia japonica. iScience [Internet]. 2018 [cited 12 nov 2020];10:135-148. Available from: https://www.sciencedirect.com/science/article/pii/S2589004218302116 Pellettieri J, Sánchez A. Cell turnover and adult tissue homeostasis: from humans to planarians. Annu Rev Genet. [Internet] 2007 [cited 14 oct 2020]; 41:83-105. Available in: doi:10.1146/annurev.genet.41.110306.130244 Schaeffer DJ. Planarians as a model system for in vivo tumorigenesis studies. Ecotoxicol Environ Saf [Internet]. 1993 [cited 10 oct 2020];25(1):1–18. Available from: https://www.sciencedirect.com/science/article/abs/pii/S0147651383710018?via%3Di hub. Alonso Á, Camargo JA. Ammonia Toxicity to the Freshwater Planarian Polycelis felina: Contrasting Effects of Continuous Versus Discontinuous Exposures. Arch Environ Contam Toxicol [Internet]. 2015 [cited 10 oct 2020];68(4):689–95. Available from: https://pubmed.ncbi.nlm.nih.gov/25604922/ Best JB, Rubinstein I. Maze learning and associated behavior in planaria. J Comp Physiol Psychol [Internet]. 1962 [cited 2021 Oct 10];55(4):560–6. Available from: https://psycnet.apa.org/record/1964-00492-001 Morita M, Boyd Best J. Electron microscopic studies on planaria. II. Fine structure of the neurosecretory system in the planarian Dugesia dorotocephala. J Ultrasructure Res [Internet]. 1965 [cited 2021 Oct 10];13(5–6):396–408. Available from: https://pubmed.ncbi.nlm.nih.gov/5848838/ Arees EA. Absence of light response in eyeless planaria. Physiol Behav [Internet]. 1986 [cited 2021 Oct 10];36(3):445–9. Available from: https://pubmed.ncbi.nlm.nih.gov/3703973/ Rivera VR, Perich MJ. Effects of water quality on survival and reproduction of four species of planaria (Turbellaria: Tricladida). Invertebr Reprod Dev [Internet]. 1994[cited 2021 Oct 10];25(1):1–7. Available from: https://www.tandfonline.com/doi/abs/10.1080/07924259.1994.9672362 Dasheiff BD, Dasheiff RM. Photonegative response in brown planaria (Dugesia tigrina) following regeneration. Ecotoxicol Environ Saf [Internet]. 2002;[cited 2021 Oct 10] 53(2):196–9. Available from: https://www.sciencedirect.com/science/article/abs/pii/S014765130292227X Lombardo P, Giustini M, Miccoli FP, Cicolani B. Fine-scale differences in diel activity among nocturnal freshwater planarians (Platyhelminthes: Tricladida). J Circadian Rhythms [Internet]. 2011[cited 2021 Oct 10];9:1–10. Available from: https://jcircadianrhythms.biomedcentral.com/articles/10.1186/1740-3391-9-2 Bocchinfuso DG, Taylor P, Ross E, Ignatchenko A, Ignatchenko V, Kislinger T, et al. Proteomic profiling of the planarian Schmidtea mediterranea and its mucous reveals similarities with human secretions and those predicted for parasitic flatworms. Mol Cell Proteomics [Internet]. 2012; [cited 2021 Oct 10]11(9):681–91. Available from: https://www.mcponline.org/article/S1535-9476(20)32581-0/fulltext# Abnave P, Mottola G, Gimenez G, Boucherit N, Trouplin V, Torre C, et al. Screening in planarians identifies MORN2 as a key component in LC3-associated phagocytosis and resistance to bacterial infection. Cell Host Microbe [Internet]. 2014 [cited 2021 Oct 10];16(3):338–50. Available from: http://dx.doi.org/10.1016/j.chom.2014.08.002 Akiyama Y, Agata K, Inoue T. Spontaneous behaviors and wall-curvature lead to apparent wall preference in planarian. PLoS One [Internet]. 2015 [cited 2021 Oct 11];10(11):1–17. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0142214 .Lewallen M, Burggren W. Metabolic physiology of the freshwater planaria girardia dorotocephela and Schmidtea mediterranea: Reproductive mode, specific dynamic action, and temperature. Am J Physiol - Regul Integr Comp Physiol [Internet]. 2020 [cited 2021 Oct 15];319(4):R428–38. Available from: https://journals.physiology.org/doi/pdf/10.1152/ajpregu.00099.2020 Collins JJ, Hou X, Romanova EV, Lambrus BG, Miller CM, Saberi A et al. GenomeWide Analyses Reveal a Role for Peptide Hormones in Planarian Germline Development. PLoS Biology [Internet]. 2010 [cited 12 nov 2020];8(10). Available from: https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1000509 Deochand N, Costello MS, Deochand ME. Behavioral Research with Planaria. Perspect Behav Sci [Internet]. 2018 [cited 2021 Mar] 24;41(2):447–64. Available from: https://www.researchgate.net/publication/328840677_Behavioral_Research_with_Pl anaria Muñoz MA, Vélez I. Redescripción y algunos aspectos ecológicos de Girardia tigrina, G. cameliae y G. paramensis (Dugesiidae, Tricladida) en Antioquia, Colombia. Rev Mex Biodivers [Internet]. 2007;78(2):291–301. Available from: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870- 34532007000200006 Aquanovel. Aquanovel. [Online]. Available from: http://aquanovel.com/control-deplanarias-platelmintos-en-el-acuario/ [Accessed 24 March 2021] Rink JC. Planarian Regeneration: Methods and Protocols (Methods in Molecular Biology 1774) [Internet]. Rink JC, editor. Springer New York; Humana Press; 2018. 574 p. Available from: http://www.springer.com/series/7651 Brusa F, Negrete L, Herrera-Martínez Y, Herrando-Pérez S. Girardia festae (Borelli, 1898) (Platyhelminthes: Tricladida: Dugesiidae): Distribution extension in a high-altitude lake from Colombia. Check List [Internet]. 2012;8(2):276–9. Available from: https://www.researchgate.net/publication/239731364_Girardia_festae_Borelli_1898_ Platyhelminthes_Tricladida_Dugesiidae_Distribution_extension_in_a_highaltitude_lake_from_Colombia Protocol 1: Planarian maintenance | Planarian Educational Resource [Internet]. Cuttingclass.stowers.org. 2021 [cited 21 March 2021]. Available from: https://cuttingclass.stowers.org/es/node/121413 Diagrama de representación de la anatomía de una planaria. Issigonis M, Newmark P. From worm to germ: Germ cell development and regeneration in planarians. Current Topics in Developmental Biology [Internet]. 2019 [cited 11 nov 2020]; 135:127-153. Available from: https://www.sciencedirect.com/science/article/abs/pii/S0070215319300389 Bowen ID, Ryder TA, Thompson JA. The fine structure of the planarian Polycelis tenuis Iijima. Protoplasma [Internet]. 1974 [cited 12 nov 2020]; 79:1–17. Available in: https://doi.org/10.1007/BF02055779 Hyman LH. The invertebrates. Platyhelminthes and Rhynchocoela, the acoelomate Bilateria. Vol 2. New York. McGraw-Hill [Internet]. 1951 [cited 12 nov 2020]. Available in: ISBN: 0070316619 Durant F, Lobo D, Hammelman J, Levin M. Physiological controls of large‐scale patterning in planarian regeneration: a molecular and computational perspective on growth and form. Regeneration [Internet]. 2016 [cited 12 nov 2020];3(2):78-102. Available from: https://www.researchgate.net/publication/301712239_Physiological_controls_of_larg escale_patterning_in_planarian_regeneration_a_molecular_and_computational_persp ective_on_growth_and_form Pedersen KJ. Cytological studies on the planarian neoblast. Zeitschrift für Zellforsch und Mikroskopische Anat [Internet]. 1959 [cited 2021 Sep 20];50(6):799– 817. Available from: https://link.springer.com/article/10.1007/BF00342367 Ruiz K. Validación de métodos de ensayo para determinación de pH, Conductividad, Sólidos Sedimentables, Sólidos Suspendidos y Sólidos disueltos en aguas en el Laboratorio Ambiental Environovalab. J Chem Inf Model [Internet]. 2018;127. Available from: http://www.dspace.uce.edu.ec/bitstream/25000/15362/1/TUCE-0017-0095-2018.pdf De Vos P, Garrity G, Jones D, Krieg N, Ludwig W, Rainey F et al. BERGEY’S MANUAL OF Systematic Bacteriology. 2nd ed. Springer; 2009. Becton Dickinson & Company.Crystal SBBL. Sistemas BBL Crystal. Equipo para la identificación de patógenos entéricos/no fermentantes 2012;31–8. Available from: http://winklerltda.cl/quimicav2/wp-content/uploads/2017/04/entericos.pdf . Becton Dickinson & Company. Sistemas de Identificación BBL Crystal ID. Equipo para la identificación de bacterias grampositivas. 2015;3(Id):3–8. Available from: http://winklerltda.cl/quimicav2/wp-content/uploads/2017/04/grampositivos.pdf Rink J. Stem cell systems and regeneration in planaria. Development Genes and Evolution. 2012 [cited 2022 January 20];223(1-2):67-84. de Oliveira M, Lopes K, Leite P, Morais F, de Campos Velho N. Physiological evaluation of the behavior and epidermis of freshwater planarians (Girardia tigrina and Girardia sp.) exposed to stressors. Biology Open. 2018 [cited 2022 January 20];7(6). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031348/ An M, Choi C. Activity of antioxidant enzymes and physiological responses in ark shell, Scapharca broughtonii, exposed to thermal and osmotic stress: Effects on hemolymph and biochemical parameters. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2010 [cited 2022 January 20];155(1):34- 42. Available from: https://www.sciencedirect.com/science/article/abs/pii/S1096495909002358 Huey R, Stevenson R. Integrating Thermal Physiology and Ecology of Ectotherms: A Discussion of Approaches. American Zoologist [Internet]. 1979 [cited 20 January 2022];19(1):357-366. Available from: https://academic.oup.com/icb/article/19/1/357/174499?login=true Hammoudi N, Torre C, Ghigo E, Drancourt M. Temperature affects the biology of Schmidtea mediterranea. Scientific Reports [Internet]. 2018 [cited 20 January 2022];8(1). Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175859/#CR13 Abnave P, Mottola G, Gimenez G, Boucherit N, Trouplin V, Torre C et al. Screening in Planarians Identifies MORN2 as a Key Component in LC3-Associated Phagocytosis and Resistance to Bacterial Infection. Cell Host & Microbe [Internet]. 2014 [cited 20 January 2022];16(3):338-350. Available from: https://www.cell.com/cell-hostmicrobe/fulltext/S1931-3128(14)00293- 5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1 931312814002935%3Fshowall%3Dtrue Chapman CCP, Robinson M, Mcpherson FC, Dwyer J. TOTAL DISSOLVED SOLIDS (TDS) STUDY – TASK 1 – LITERATURE REVIEW – FINAL. 2011;1(604). Available from: https://registry.mvlwb.ca/Documents/MV2011L2-0004/MV2011L2- 0004 - De Beers Snap Lake - April 2011 TDS Literature Review by Golder -Aug18- 11.PDF Ministerio de Salud -. Decreto 0475 de 1998 [Internet]. Diario Oficial 1998 p. 14. Available from: http://www.anla.gov.co/sites/default/files/normativa_ambiental/dec_0475- 98_normas_tecnicas_sobre_calidad_del_agua_potable.pdf De Vos P. Bergey's manual of systematic bacteriology. 2nd ed. Dordrecht: Springer; 2009. Park H, Bideshi D, Federici B. Properties and applied use of the mosquitocidal bacterium, Bacillus sphaericus. Journal of Asia-Pacific Entomology [Internet]. 2010 [cited 21 January 2022];13(3):159-168. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584542/ Suckow M, Stevens K, Wilson R. The laboratory rabbit, guinea pig, hamster, and other rodents. Oxford: Academic Press; 2012. Remington J. Infectious Diseases of the Fetus and Newborn Infant (Seventh Edition). 7th ed. W B Saunders Company; 2011. 0. Babar Z, Awaisu A, Chen T. Encyclopedia of pharmacy practice and clinical pharmacy. Academic Press; 2019. Fischer M, Long S, Prober C. Principles and practice of pediatric infectious diseases. 5th ed. Elsevier; 2018. Von Graevenitz A. Rothia dentocariosa: taxonomy and differential diagnosis. Clinical Microbiology and Infection. 2004 [cited 21 January 2022];10(5):399-402. Available from: https://www.sciencedirect.com/science/article/pii/S1198743X14628251 Franconieri F, Join-Lambert O, Creveuil C, Auzou M, Labombarda F, Aouba A et al. Rothia spp. infective endocarditis: A systematic literature review. Infectious Diseases Now [Internet]. 2021 [cited 21 January 2022];51(3):228-235. Available from: https://www.sciencedirect.com/science/article/pii/S0399077X20307617#bbib0900 Salimiyan Rizi K, Farsiani H, Ghazvini K, Youssefi M. Peritonitis Due to Rothia dentocariosa in Iran: A Case Report. Reviews in Clinical Medicine [Internet]. 2019 [cited 21 January 2022];6(1). Available from: http://eprints.mums.ac.ir/11400/1/RCM_Volume%206_Issue%201_Pages%2037- 40.pdf Boudewijns M, Magerman K, Verhaegen J, Debrock G, Peetermans W, Donkersloot P et al. Rothia dentocariosa, endocarditis and mycotic aneurysms: case report and review of the literature. Clinical Microbiology and Infection [Internet]. 2003 [cited 21 January 2022];9(3):222-229. Available from: https://www.sciencedirect.com/science/article/pii/S1198743X1462471X#tbl3 Sakurada Z A. Chryseobacterium indologenes [Internet]. Scielo. 2008 [cited 22 January 2022]. Available from: https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182008000600005 Rojas-Rojas F, López-Sánchez D, Meza-Radilla G, Méndez-Canarios A, Ibarra J, Estrada-de los Santos P. El controvertido complejo Burkholderia cepacia, un grupo de especies promotoras del crecimiento vegetal y patógenas de plantas, animales y humanos. Revista Argentina de Microbiología [Internet]. 2019 [cited 22 January 2022];51(1):84-92. Available from: https://www.sciencedirect.com/science/article/pii/S0325754118300038 Cabrales Escobar I, Juárez Cruz A. Bacteriemia asociada a catéter por Kluyvera cryocrescens: reporte de caso y revisión. Enfermedades Infecciosas y Microbiología [Internet]. 2021 [cited 21 January 2022];41(1):35-38. Available from: https://www.medigraphic.com/pdfs/micro/ei-2021/ei211g.pdf Barón S. Medical Microbiology. 4th ed. Galveston: University of Texas; 1996 |
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Derechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2022 |
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https://creativecommons.org/licenses/by-nc-sa/4.0/ |
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Derechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2022 https://creativecommons.org/licenses/by-nc-sa/4.0/ Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) http://purl.org/coar/access_right/c_14cb |
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Facultad de Ciencias de la Salud |
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Bogotá |
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Bacteriología y Laboratorio Clínico |
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Sánchez Leal, Ligia Consuelo3285fc07f487979b774cdc46534973da600Gonzalez Fontecha, Jossie Esteban9a60ece2e3f218a867f6030674564803Patiño Herrera, Ariana Yineth8f67f5b817bd07e145ddeca6cce2afa06002022-10-18T13:49:58Z2022-10-18T13:49:58Z2022-04https://repositorio.unicolmayor.edu.co/handle/unicolmayor/5708Las planarias, también llamados gusanos planos, son organismos triploblásticos, acoelomatosos, pertenecientes al filo de platelmintos, los cuales se han estudiado ampliamente en áreas de medicina regenerativa y la biología del desarrollo a causa de su gran capacidad de regeneración de tejido a partir de sus células madre llamadas neoblastos. A pesar de ser un modelo biológico con gran bioprospección no se han estudiado en conjunto los factores bióticos y abióticos que intervienen en su biología y comportamiento. En el presente proyecto se estudiaron los factores bióticos y abióticos que pueden tener algún efecto en el establecimiento y el mantenimiento de una cría de planarias. Se caracterizó la microbiota externa de las planarias, la población bacteriana las piedras, la materia orgánica de la que se alimentan las planarias y el agua de la quebrada La Vieja y se verificó la vitalidad y el comportamiento de las planarias en el establecimiento de su cría en el laboratorio. Se evidenció que las planarias son metazoos con gran capacidad de adaptación a diferentes factores bióticos y abióticos, tolerando cambios de temperatura, conductividad y TDS. Por otra parte, son organismos que albergan una amplia variedad microbiana, y conviven con una gran diversidad de bacterias en su hábitat natural. De esta manera se pudo determinar que son organismos viables para el cultivo en un ambiente controlado de laboratorio, condiciones adecuadas de alimentación, temperatura y otras características que indican la continuidad de una población estable en el tiempo, y de hábitos y comportamiento definidosTabla de Contenido Resumen 9 Introducción 11 Objetivo general 13 Objetivos específicos 13 1. Antecedentes 14 2. Marco referencial 20 2.1. Generalidades de las Planarias 20 2.2. Anatomía y Fisiología 21 2.3. Características y distribución de los neoblastos 22 2.4. Factores Bióticos 25 2.5. Factores Abióticos 27 3. Diseño metodológico 29 3.1. Universo, población y muestra 29 3.2. Hipótesis, variables, indicadores 29 3.3. Técnicas y procedimientos 29 4. Resultados 41 5. Discusión 47 6. Conclusiones 57 Referencias Bibliográficas 58PregradoBacteriólogo(a) y Laboratorista Clínico69p.application/pdfspaDerechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2022https://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/closedAccessAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)http://purl.org/coar/access_right/c_14cbEvaluación de los factores bióticos y abióticos en una cría de planariasTrabajo de grado - Pregradohttp://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85Textinfo:eu-repo/semantics/bachelorThesishttps://purl.org/redcol/resource_type/TPinfo:eu-repo/semantics/publishedVersionFacultad de Ciencias de la SaludBogotáBacteriología y Laboratorio ClínicoIvanovic Z, Vlaski-Lafarge M. Metabolic and Genetic Features of Ancestral Eukaryotes versus Metabolism and “Master Pluripotency Genes” of Stem Cells. Anaerobiosis and Stemness [Internet]. 2016 [cited 24 Mar 2021 ];:211-234. Available from: doi: 10.1016/B978-0-12-800540-8.00011-9 https://www.sciencedirect.com/science/article/pii/B9780128005408000119Cabej NR. Cambrian explosion. In: Cabej NR, editor. Epigenetic Mechanisms of the Cambrian Explosion [Internet]. 2020. [cited 24Mar 2021] p. 137–211. Available from: https://www.sciencedirect.com/science/article/pii/B9780128143117000044Foster JA. Induction of Neoplasms in Planarians with Carcinogens. Cancer Res. [Internet]. 1963 [cited 10 oct 2020]; 23:300–3. Available in: https://cancerres.aacrjournals.org/content/23/2_Part_1/300.longSchaeffer DJ. Planarians as a model system for in vivo tumorigenesis studies. Ecotoxicol Environ Saf. [Internet]. 1993 [cited 24 Mar 2021]; 25(1):1-18. Available in:https://www.sciencedirect.com/science/article/abs/pii/S0147651383710018?via%3 Dihub doi:10.1006/eesa.1993.1001Arnold CP, Merryman MS, Harris AA, McKinney S, Seidel C, Loethen S et al. Pathogenic shifts in endogenous microbiota impede tissue regeneration via distinct activation of TAK1/MKK/p38. eLife [Internet]. 2016 [cited 24 Mar 2021];5. Available from: https://pubmed.ncbi.nlm.nih.gov/27441386/Rompolas P, Patel-King RS, King SM. Schmidtea mediterranea: a model system for analysis of motile cilia. In: Methods in cell biology [Internet]. First edit. Elsevier; 2009 [cited 24 Mar 2021]. p. 81–98. Available from: http://dx.doi.org/10.1016/S0091- 679X(08)93004-1. Legner EF, Tsai TC, Medved RA. Environmental stimulants to asexual reproduction in the planarian,Dugesia dorotocephala. Biol Control [Internet]. 1976;[cited 27 Mar 2021]21(4):415–23. Available from: https://link.springer.com/article/10.1007/BF02371640. Lee F, Williams K, Levin M, Wolfe B. The Bacterial Metabolite Indole Inhibits Regeneration of the Planarian Flatworm Dugesia japonica. iScience [Internet]. 2018 [cited 12 nov 2020];10:135-148. Available from: https://www.sciencedirect.com/science/article/pii/S2589004218302116Pellettieri J, Sánchez A. Cell turnover and adult tissue homeostasis: from humans to planarians. Annu Rev Genet. [Internet] 2007 [cited 14 oct 2020]; 41:83-105. Available in: doi:10.1146/annurev.genet.41.110306.130244Schaeffer DJ. Planarians as a model system for in vivo tumorigenesis studies. Ecotoxicol Environ Saf [Internet]. 1993 [cited 10 oct 2020];25(1):1–18. Available from: https://www.sciencedirect.com/science/article/abs/pii/S0147651383710018?via%3Di hub.Alonso Á, Camargo JA. Ammonia Toxicity to the Freshwater Planarian Polycelis felina: Contrasting Effects of Continuous Versus Discontinuous Exposures. Arch Environ Contam Toxicol [Internet]. 2015 [cited 10 oct 2020];68(4):689–95. Available from: https://pubmed.ncbi.nlm.nih.gov/25604922/Best JB, Rubinstein I. Maze learning and associated behavior in planaria. J Comp Physiol Psychol [Internet]. 1962 [cited 2021 Oct 10];55(4):560–6. Available from: https://psycnet.apa.org/record/1964-00492-001Morita M, Boyd Best J. Electron microscopic studies on planaria. II. Fine structure of the neurosecretory system in the planarian Dugesia dorotocephala. J Ultrasructure Res [Internet]. 1965 [cited 2021 Oct 10];13(5–6):396–408. Available from: https://pubmed.ncbi.nlm.nih.gov/5848838/Arees EA. Absence of light response in eyeless planaria. Physiol Behav [Internet]. 1986 [cited 2021 Oct 10];36(3):445–9. Available from: https://pubmed.ncbi.nlm.nih.gov/3703973/Rivera VR, Perich MJ. Effects of water quality on survival and reproduction of four species of planaria (Turbellaria: Tricladida). Invertebr Reprod Dev [Internet]. 1994[cited 2021 Oct 10];25(1):1–7. Available from: https://www.tandfonline.com/doi/abs/10.1080/07924259.1994.9672362Dasheiff BD, Dasheiff RM. Photonegative response in brown planaria (Dugesia tigrina) following regeneration. Ecotoxicol Environ Saf [Internet]. 2002;[cited 2021 Oct 10] 53(2):196–9. Available from: https://www.sciencedirect.com/science/article/abs/pii/S014765130292227XLombardo P, Giustini M, Miccoli FP, Cicolani B. Fine-scale differences in diel activity among nocturnal freshwater planarians (Platyhelminthes: Tricladida). J Circadian Rhythms [Internet]. 2011[cited 2021 Oct 10];9:1–10. Available from: https://jcircadianrhythms.biomedcentral.com/articles/10.1186/1740-3391-9-2Bocchinfuso DG, Taylor P, Ross E, Ignatchenko A, Ignatchenko V, Kislinger T, et al. Proteomic profiling of the planarian Schmidtea mediterranea and its mucous reveals similarities with human secretions and those predicted for parasitic flatworms. Mol Cell Proteomics [Internet]. 2012; [cited 2021 Oct 10]11(9):681–91. Available from: https://www.mcponline.org/article/S1535-9476(20)32581-0/fulltext#Abnave P, Mottola G, Gimenez G, Boucherit N, Trouplin V, Torre C, et al. Screening in planarians identifies MORN2 as a key component in LC3-associated phagocytosis and resistance to bacterial infection. Cell Host Microbe [Internet]. 2014 [cited 2021 Oct 10];16(3):338–50. Available from: http://dx.doi.org/10.1016/j.chom.2014.08.002Akiyama Y, Agata K, Inoue T. Spontaneous behaviors and wall-curvature lead to apparent wall preference in planarian. PLoS One [Internet]. 2015 [cited 2021 Oct 11];10(11):1–17. Available from: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0142214.Lewallen M, Burggren W. Metabolic physiology of the freshwater planaria girardia dorotocephela and Schmidtea mediterranea: Reproductive mode, specific dynamic action, and temperature. Am J Physiol - Regul Integr Comp Physiol [Internet]. 2020 [cited 2021 Oct 15];319(4):R428–38. Available from: https://journals.physiology.org/doi/pdf/10.1152/ajpregu.00099.2020Collins JJ, Hou X, Romanova EV, Lambrus BG, Miller CM, Saberi A et al. GenomeWide Analyses Reveal a Role for Peptide Hormones in Planarian Germline Development. PLoS Biology [Internet]. 2010 [cited 12 nov 2020];8(10). Available from: https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1000509Deochand N, Costello MS, Deochand ME. Behavioral Research with Planaria. Perspect Behav Sci [Internet]. 2018 [cited 2021 Mar] 24;41(2):447–64. Available from: https://www.researchgate.net/publication/328840677_Behavioral_Research_with_Pl anariaMuñoz MA, Vélez I. Redescripción y algunos aspectos ecológicos de Girardia tigrina, G. cameliae y G. paramensis (Dugesiidae, Tricladida) en Antioquia, Colombia. Rev Mex Biodivers [Internet]. 2007;78(2):291–301. Available from: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1870- 34532007000200006Aquanovel. Aquanovel. [Online]. Available from: http://aquanovel.com/control-deplanarias-platelmintos-en-el-acuario/ [Accessed 24 March 2021]Rink JC. Planarian Regeneration: Methods and Protocols (Methods in Molecular Biology 1774) [Internet]. Rink JC, editor. Springer New York; Humana Press; 2018. 574 p. Available from: http://www.springer.com/series/7651Brusa F, Negrete L, Herrera-Martínez Y, Herrando-Pérez S. Girardia festae (Borelli, 1898) (Platyhelminthes: Tricladida: Dugesiidae): Distribution extension in a high-altitude lake from Colombia. Check List [Internet]. 2012;8(2):276–9. Available from: https://www.researchgate.net/publication/239731364_Girardia_festae_Borelli_1898_ Platyhelminthes_Tricladida_Dugesiidae_Distribution_extension_in_a_highaltitude_lake_from_ColombiaProtocol 1: Planarian maintenance | Planarian Educational Resource [Internet]. Cuttingclass.stowers.org. 2021 [cited 21 March 2021]. Available from: https://cuttingclass.stowers.org/es/node/121413Diagrama de representación de la anatomía de una planaria. Issigonis M, Newmark P. From worm to germ: Germ cell development and regeneration in planarians. Current Topics in Developmental Biology [Internet]. 2019 [cited 11 nov 2020]; 135:127-153. Available from: https://www.sciencedirect.com/science/article/abs/pii/S0070215319300389Bowen ID, Ryder TA, Thompson JA. The fine structure of the planarian Polycelis tenuis Iijima. Protoplasma [Internet]. 1974 [cited 12 nov 2020]; 79:1–17. Available in: https://doi.org/10.1007/BF02055779Hyman LH. The invertebrates. Platyhelminthes and Rhynchocoela, the acoelomate Bilateria. Vol 2. New York. McGraw-Hill [Internet]. 1951 [cited 12 nov 2020]. Available in: ISBN: 0070316619Durant F, Lobo D, Hammelman J, Levin M. Physiological controls of large‐scale patterning in planarian regeneration: a molecular and computational perspective on growth and form. Regeneration [Internet]. 2016 [cited 12 nov 2020];3(2):78-102. Available from: https://www.researchgate.net/publication/301712239_Physiological_controls_of_larg escale_patterning_in_planarian_regeneration_a_molecular_and_computational_persp ective_on_growth_and_formPedersen KJ. Cytological studies on the planarian neoblast. Zeitschrift für Zellforsch und Mikroskopische Anat [Internet]. 1959 [cited 2021 Sep 20];50(6):799– 817. Available from: https://link.springer.com/article/10.1007/BF00342367Ruiz K. Validación de métodos de ensayo para determinación de pH, Conductividad, Sólidos Sedimentables, Sólidos Suspendidos y Sólidos disueltos en aguas en el Laboratorio Ambiental Environovalab. J Chem Inf Model [Internet]. 2018;127. Available from: http://www.dspace.uce.edu.ec/bitstream/25000/15362/1/TUCE-0017-0095-2018.pdfDe Vos P, Garrity G, Jones D, Krieg N, Ludwig W, Rainey F et al. BERGEY’S MANUAL OF Systematic Bacteriology. 2nd ed. Springer; 2009.Becton Dickinson & Company.Crystal SBBL. Sistemas BBL Crystal. Equipo para la identificación de patógenos entéricos/no fermentantes 2012;31–8. Available from: http://winklerltda.cl/quimicav2/wp-content/uploads/2017/04/entericos.pdf. Becton Dickinson & Company. Sistemas de Identificación BBL Crystal ID. Equipo para la identificación de bacterias grampositivas. 2015;3(Id):3–8. Available from: http://winklerltda.cl/quimicav2/wp-content/uploads/2017/04/grampositivos.pdfRink J. Stem cell systems and regeneration in planaria. Development Genes and Evolution. 2012 [cited 2022 January 20];223(1-2):67-84.de Oliveira M, Lopes K, Leite P, Morais F, de Campos Velho N. Physiological evaluation of the behavior and epidermis of freshwater planarians (Girardia tigrina and Girardia sp.) exposed to stressors. Biology Open. 2018 [cited 2022 January 20];7(6). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031348/An M, Choi C. Activity of antioxidant enzymes and physiological responses in ark shell, Scapharca broughtonii, exposed to thermal and osmotic stress: Effects on hemolymph and biochemical parameters. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2010 [cited 2022 January 20];155(1):34- 42. Available from: https://www.sciencedirect.com/science/article/abs/pii/S1096495909002358Huey R, Stevenson R. Integrating Thermal Physiology and Ecology of Ectotherms: A Discussion of Approaches. American Zoologist [Internet]. 1979 [cited 20 January 2022];19(1):357-366. Available from: https://academic.oup.com/icb/article/19/1/357/174499?login=trueHammoudi N, Torre C, Ghigo E, Drancourt M. Temperature affects the biology of Schmidtea mediterranea. Scientific Reports [Internet]. 2018 [cited 20 January 2022];8(1). Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175859/#CR13Abnave P, Mottola G, Gimenez G, Boucherit N, Trouplin V, Torre C et al. Screening in Planarians Identifies MORN2 as a Key Component in LC3-Associated Phagocytosis and Resistance to Bacterial Infection. Cell Host & Microbe [Internet]. 2014 [cited 20 January 2022];16(3):338-350. Available from: https://www.cell.com/cell-hostmicrobe/fulltext/S1931-3128(14)00293- 5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1 931312814002935%3Fshowall%3DtrueChapman CCP, Robinson M, Mcpherson FC, Dwyer J. TOTAL DISSOLVED SOLIDS (TDS) STUDY – TASK 1 – LITERATURE REVIEW – FINAL. 2011;1(604). Available from: https://registry.mvlwb.ca/Documents/MV2011L2-0004/MV2011L2- 0004 - De Beers Snap Lake - April 2011 TDS Literature Review by Golder -Aug18- 11.PDFMinisterio de Salud -. Decreto 0475 de 1998 [Internet]. Diario Oficial 1998 p. 14. Available from: http://www.anla.gov.co/sites/default/files/normativa_ambiental/dec_0475- 98_normas_tecnicas_sobre_calidad_del_agua_potable.pdfDe Vos P. Bergey's manual of systematic bacteriology. 2nd ed. Dordrecht: Springer; 2009.Park H, Bideshi D, Federici B. Properties and applied use of the mosquitocidal bacterium, Bacillus sphaericus. Journal of Asia-Pacific Entomology [Internet]. 2010 [cited 21 January 2022];13(3):159-168. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5584542/Suckow M, Stevens K, Wilson R. The laboratory rabbit, guinea pig, hamster, and other rodents. Oxford: Academic Press; 2012.Remington J. Infectious Diseases of the Fetus and Newborn Infant (Seventh Edition). 7th ed. W B Saunders Company; 2011.0. Babar Z, Awaisu A, Chen T. Encyclopedia of pharmacy practice and clinical pharmacy. Academic Press; 2019.Fischer M, Long S, Prober C. Principles and practice of pediatric infectious diseases. 5th ed. Elsevier; 2018.Von Graevenitz A. Rothia dentocariosa: taxonomy and differential diagnosis. Clinical Microbiology and Infection. 2004 [cited 21 January 2022];10(5):399-402. Available from: https://www.sciencedirect.com/science/article/pii/S1198743X14628251Franconieri F, Join-Lambert O, Creveuil C, Auzou M, Labombarda F, Aouba A et al. Rothia spp. infective endocarditis: A systematic literature review. Infectious Diseases Now [Internet]. 2021 [cited 21 January 2022];51(3):228-235. Available from: https://www.sciencedirect.com/science/article/pii/S0399077X20307617#bbib0900Salimiyan Rizi K, Farsiani H, Ghazvini K, Youssefi M. Peritonitis Due to Rothia dentocariosa in Iran: A Case Report. Reviews in Clinical Medicine [Internet]. 2019 [cited 21 January 2022];6(1). Available from: http://eprints.mums.ac.ir/11400/1/RCM_Volume%206_Issue%201_Pages%2037- 40.pdfBoudewijns M, Magerman K, Verhaegen J, Debrock G, Peetermans W, Donkersloot P et al. Rothia dentocariosa, endocarditis and mycotic aneurysms: case report and review of the literature. Clinical Microbiology and Infection [Internet]. 2003 [cited 21 January 2022];9(3):222-229. Available from: https://www.sciencedirect.com/science/article/pii/S1198743X1462471X#tbl3Sakurada Z A. Chryseobacterium indologenes [Internet]. Scielo. 2008 [cited 22 January 2022]. Available from: https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-10182008000600005Rojas-Rojas F, López-Sánchez D, Meza-Radilla G, Méndez-Canarios A, Ibarra J, Estrada-de los Santos P. El controvertido complejo Burkholderia cepacia, un grupo de especies promotoras del crecimiento vegetal y patógenas de plantas, animales y humanos. Revista Argentina de Microbiología [Internet]. 2019 [cited 22 January 2022];51(1):84-92. Available from: https://www.sciencedirect.com/science/article/pii/S0325754118300038Cabrales Escobar I, Juárez Cruz A. Bacteriemia asociada a catéter por Kluyvera cryocrescens: reporte de caso y revisión. Enfermedades Infecciosas y Microbiología [Internet]. 2021 [cited 21 January 2022];41(1):35-38. Available from: https://www.medigraphic.com/pdfs/micro/ei-2021/ei211g.pdfBarón S. Medical Microbiology. 4th ed. 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Unicolmayorrepositorio@unicolmayor.edu.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