Diversidad genética de Pseudomonas aeruginosa y su relación con la resistencia bacteriana y producción de biopelículas en la ciudad de Cartagena de Indias D.C.T.-Colombia

P. aeruginosa es un patógeno oportunista que representa un problema de salud pública, siendo uno de microorganismos frecuentemente aislado en casos de infecciones asociadas a la atención en salud y que expresa resistencia a la mayoría de los antibióticos de uso rutinario. En este trabajo, realizamos...

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Autores:
Valle Molinares, Roger Humberto
Tipo de recurso:
Doctoral thesis
Fecha de publicación:
2020
Institución:
Universidad de Cartagena
Repositorio:
Repositorio Universidad de Cartagena
Idioma:
spa
OAI Identifier:
oai:repositorio.unicartagena.edu.co:11227/15503
Acceso en línea:
https://hdl.handle.net/11227/15503
http://dx.doi.org/10.57799/11227/147
Palabra clave:
Microorganismos
Antibióticos - Efectos fisiológicos
Antibióticos
Microorganismo - Efecto de los antibióticos
Bacterias - Fisiología
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openAccess
License
Derechos Reservados - Universidad de Cartagena, 2020
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network_acronym_str UCART2
network_name_str Repositorio Universidad de Cartagena
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dc.title.spa.fl_str_mv Diversidad genética de Pseudomonas aeruginosa y su relación con la resistencia bacteriana y producción de biopelículas en la ciudad de Cartagena de Indias D.C.T.-Colombia
title Diversidad genética de Pseudomonas aeruginosa y su relación con la resistencia bacteriana y producción de biopelículas en la ciudad de Cartagena de Indias D.C.T.-Colombia
spellingShingle Diversidad genética de Pseudomonas aeruginosa y su relación con la resistencia bacteriana y producción de biopelículas en la ciudad de Cartagena de Indias D.C.T.-Colombia
Microorganismos
Antibióticos - Efectos fisiológicos
Antibióticos
Microorganismo - Efecto de los antibióticos
Bacterias - Fisiología
title_short Diversidad genética de Pseudomonas aeruginosa y su relación con la resistencia bacteriana y producción de biopelículas en la ciudad de Cartagena de Indias D.C.T.-Colombia
title_full Diversidad genética de Pseudomonas aeruginosa y su relación con la resistencia bacteriana y producción de biopelículas en la ciudad de Cartagena de Indias D.C.T.-Colombia
title_fullStr Diversidad genética de Pseudomonas aeruginosa y su relación con la resistencia bacteriana y producción de biopelículas en la ciudad de Cartagena de Indias D.C.T.-Colombia
title_full_unstemmed Diversidad genética de Pseudomonas aeruginosa y su relación con la resistencia bacteriana y producción de biopelículas en la ciudad de Cartagena de Indias D.C.T.-Colombia
title_sort Diversidad genética de Pseudomonas aeruginosa y su relación con la resistencia bacteriana y producción de biopelículas en la ciudad de Cartagena de Indias D.C.T.-Colombia
dc.creator.fl_str_mv Valle Molinares, Roger Humberto
dc.contributor.advisor.none.fl_str_mv Gómez Camargo, Doris Esther
dc.contributor.author.none.fl_str_mv Valle Molinares, Roger Humberto
dc.subject.armarc.none.fl_str_mv Microorganismos
Antibióticos - Efectos fisiológicos
Antibióticos
Microorganismo - Efecto de los antibióticos
Bacterias - Fisiología
topic Microorganismos
Antibióticos - Efectos fisiológicos
Antibióticos
Microorganismo - Efecto de los antibióticos
Bacterias - Fisiología
description P. aeruginosa es un patógeno oportunista que representa un problema de salud pública, siendo uno de microorganismos frecuentemente aislado en casos de infecciones asociadas a la atención en salud y que expresa resistencia a la mayoría de los antibióticos de uso rutinario. En este trabajo, realizamos un análisis de los perfiles de susceptibilidad, producción de biopelículas y expresión de genes asociados a resistencia antibiótica, de un total de 60 aislados (56 de origen clínico y 4 de origen ambiental). Los resultados obtenidos permitieron realizar una descripción fenotípica y genotípica de la población de P. aeruginosa que circula en la ciudad de Cartagena. La mayoría de los aislamientos mostraron elevados niveles de resistencia (30% MDR, 32% XDR y 15% PDR). Así mismo, se encontró que los marcadores de resistencia blaTEM y blaSHV son los más frecuentes en la población de P. aeruginosa con frecuencias de aparición del 26% y 100% respectivamente. Por otro lado, en este trabajo aportamos evidencia de la sobreexpresión en la biopelícula de los genes blaSHV lo que puede ser de utilidad a la hora es escoger el tratamiento antibiótico más adecuado para el manejo de infecciones crónicas de P. aeruginosa. También, aportamos información preliminar sobre el potencial uso de moléculas de origen natural (extractos crudos de animales marinos) y sintéticos (Tedrahydroquinolinas) en el tratamiento de infecciones de P. aeruginosa.
publishDate 2020
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dc.date.accessioned.none.fl_str_mv 2022-07-28T18:28:12Z
dc.date.available.none.fl_str_mv 2022-07-28T18:28:12Z
dc.type.spa.fl_str_mv Trabajo de grado - Doctorado
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dc.rights.spa.fl_str_mv Derechos Reservados - Universidad de Cartagena, 2020
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dc.publisher.spa.fl_str_mv Universidad de Cartagena
dc.publisher.faculty.spa.fl_str_mv Facultad de Medicina
dc.publisher.place.spa.fl_str_mv Cartagena de Indias
dc.publisher.program.spa.fl_str_mv Doctorado en Medicina Tropical
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spelling Gómez Camargo, Doris Estherdfad495a08442d6ad11fa7be55f7b1ffValle Molinares, Roger Humbertod1ae1acb921558a33c7d0eeba72e67672022-07-28T18:28:12Z2022-07-28T18:28:12Z2020https://hdl.handle.net/11227/15503http://dx.doi.org/10.57799/11227/147P. aeruginosa es un patógeno oportunista que representa un problema de salud pública, siendo uno de microorganismos frecuentemente aislado en casos de infecciones asociadas a la atención en salud y que expresa resistencia a la mayoría de los antibióticos de uso rutinario. En este trabajo, realizamos un análisis de los perfiles de susceptibilidad, producción de biopelículas y expresión de genes asociados a resistencia antibiótica, de un total de 60 aislados (56 de origen clínico y 4 de origen ambiental). Los resultados obtenidos permitieron realizar una descripción fenotípica y genotípica de la población de P. aeruginosa que circula en la ciudad de Cartagena. La mayoría de los aislamientos mostraron elevados niveles de resistencia (30% MDR, 32% XDR y 15% PDR). Así mismo, se encontró que los marcadores de resistencia blaTEM y blaSHV son los más frecuentes en la población de P. aeruginosa con frecuencias de aparición del 26% y 100% respectivamente. Por otro lado, en este trabajo aportamos evidencia de la sobreexpresión en la biopelícula de los genes blaSHV lo que puede ser de utilidad a la hora es escoger el tratamiento antibiótico más adecuado para el manejo de infecciones crónicas de P. aeruginosa. También, aportamos información preliminar sobre el potencial uso de moléculas de origen natural (extractos crudos de animales marinos) y sintéticos (Tedrahydroquinolinas) en el tratamiento de infecciones de P. aeruginosa.DoctoradoDoctor(a) en Medicina Tropicalapplication/pdfspaUniversidad de CartagenaFacultad de MedicinaCartagena de IndiasDoctorado en Medicina TropicalDerechos Reservados - Universidad de Cartagena, 2020https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)http://purl.org/coar/access_right/c_abf2Diversidad genética de Pseudomonas aeruginosa y su relación con la resistencia bacteriana y producción de biopelículas en la ciudad de Cartagena de Indias D.C.T.-ColombiaTrabajo de grado - Doctoradoinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_db06Textinfo:eu-repo/semantics/doctoralThesishttps://purl.org/redcol/resource_type/TDhttp://purl.org/coar/version/c_970fb48d4fbd8a85MicroorganismosAntibióticos - Efectos fisiológicosAntibióticosMicroorganismo - Efecto de los antibióticosBacterias - FisiologíaAbdel-Aziz, S.M., A, A., 2014. Bacterial Biofilm: Dispersal and Inhibition Strategies. Sch. J. Biotechnol. 1. https://doi.org/10.18875/2375-6713.1.105Abdel-Gawad, S.M., El-Gaby, M.S.A., Heiba, H.I., Aly, H.M., Ghorab, M.M., 2005. Synthesis and Radiation Stability of Some New Biologically Active Hydroquinoline and Pyrimido[4,5- b ]quinoline Derivatives. J. Chinese Chem. Soc. 52, 1227–1236. https://doi.org/10.1002/jccs.200500177Angeles, L., 1984. The Clinical Challenge of Infections Due to Pseudomonas aeruginosa. Rev. Infect. Dis. 6, 603–607.Arzanlou, M., Chai, W.C., Venter, H., 2017. Intrinsic, adaptive and acquired antimicrobial resistance in Gram-negative bacteria. Essays Biochem. 61, 49–60. https://doi.org/10.1042/EBC20160063Azam, M.W., Khan, A.U., 2019. Updates on the pathogenicity status of Pseudomonas aeruginosa. Drug Discov. Today 24, 350–359. https://doi.org/10.1016/j.drudis.2018.07.003Azimi, S., Kafil, H.S., Baghi, H.B., Shokrian, S., Najaf, K., Asgharzadeh, M., Yousefi, M., Shahrivar, F., Aghazadeh, M., 2016. Presence of exoY, exoS, exoU and exoT genes, antibiotic resistance and biofilm production among Pseudomonas aeruginosa isolates in Northwest Iran. GMS Hyg. Infect. Control 11, Doc04. https://doi.org/10.3205/dgkh000264Baena del Valle, J.A., Gomez Alegria, C.J., Gomez Camargo, D.E., 2014. Antimicrobial susceptibility and genotypification of Pseudomonas aeruginosa from cystic fibrosis patients and other diseases in Cartagena (Colombia). Salud UNINORTE 30, 104– 120.Baena Del Valle, J.A., Gómez Alegría, C.J., Gómez Camargo, D.E., 2011. Coexistencia de Pseudomonas aeruginosa y Candida albicans en infecciones nosocomiales en Cartagena de Indias (Colombia). Nov. Publicación Científica en Ciencias Biomédicas 9, 22–30.Baena, J., Gomez, C., Gomez, D., 2014. 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