Descelularización y caracterización in vitro de matrices de intestino delgado porcino para el tratamiento de heridas complejas

Introducción: las lesiones cutáneas complicadas se han convertido en un problema de salud mundial, siendo difíciles de tratar debido al limitado proceso de curación del cuerpo. Se han realizado estudios para mejorar los tratamientos tradicionales que tienen muchas desventajas. La investigación en ci...

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Autores:
Ruíz Soto, Juan Pablo
Galvis Escobar, Sara María
Rego Londoño, Maria Antonia
Molina Sierra, Juan David
Pineda Molina, Catalina
Tipo de recurso:
Article of journal
Fecha de publicación:
2023
Institución:
Universidad de Cartagena
Repositorio:
Repositorio Universidad de Cartagena
Idioma:
eng
OAI Identifier:
oai:repositorio.unicartagena.edu.co:11227/17952
Acceso en línea:
https://hdl.handle.net/11227/17952
https://doi.org/10.32997/rcb-3023-4135
Palabra clave:
bioactive scaffold
decellularization
porcine small intestine
skin wound
tissue engineering
matriz bioactiva
descelularización
intestino delgado porcino
herida de piel
ingeniería de tejidos
Rights
openAccess
License
https://creativecommons.org/licenses/by-nc-nd/4.0
id UCART2_7abc74c1a8764b39191cc68dc9d1644c
oai_identifier_str oai:repositorio.unicartagena.edu.co:11227/17952
network_acronym_str UCART2
network_name_str Repositorio Universidad de Cartagena
repository_id_str
dc.title.spa.fl_str_mv Descelularización y caracterización in vitro de matrices de intestino delgado porcino para el tratamiento de heridas complejas
dc.title.translated.eng.fl_str_mv Decellularization and in vitro characterization of porcine small intestine scaffolds for complex wound treatments
title Descelularización y caracterización in vitro de matrices de intestino delgado porcino para el tratamiento de heridas complejas
spellingShingle Descelularización y caracterización in vitro de matrices de intestino delgado porcino para el tratamiento de heridas complejas
bioactive scaffold
decellularization
porcine small intestine
skin wound
tissue engineering
matriz bioactiva
descelularización
intestino delgado porcino
herida de piel
ingeniería de tejidos
title_short Descelularización y caracterización in vitro de matrices de intestino delgado porcino para el tratamiento de heridas complejas
title_full Descelularización y caracterización in vitro de matrices de intestino delgado porcino para el tratamiento de heridas complejas
title_fullStr Descelularización y caracterización in vitro de matrices de intestino delgado porcino para el tratamiento de heridas complejas
title_full_unstemmed Descelularización y caracterización in vitro de matrices de intestino delgado porcino para el tratamiento de heridas complejas
title_sort Descelularización y caracterización in vitro de matrices de intestino delgado porcino para el tratamiento de heridas complejas
dc.creator.fl_str_mv Ruíz Soto, Juan Pablo
Galvis Escobar, Sara María
Rego Londoño, Maria Antonia
Molina Sierra, Juan David
Pineda Molina, Catalina
dc.contributor.author.spa.fl_str_mv Ruíz Soto, Juan Pablo
Galvis Escobar, Sara María
Rego Londoño, Maria Antonia
Molina Sierra, Juan David
Pineda Molina, Catalina
dc.subject.eng.fl_str_mv bioactive scaffold
decellularization
porcine small intestine
skin wound
tissue engineering
topic bioactive scaffold
decellularization
porcine small intestine
skin wound
tissue engineering
matriz bioactiva
descelularización
intestino delgado porcino
herida de piel
ingeniería de tejidos
dc.subject.spa.fl_str_mv matriz bioactiva
descelularización
intestino delgado porcino
herida de piel
ingeniería de tejidos
description Introducción: las lesiones cutáneas complicadas se han convertido en un problema de salud mundial, siendo difíciles de tratar debido al limitado proceso de curación del cuerpo. Se han realizado estudios para mejorar los tratamientos tradicionales que tienen muchas desventajas. La investigación en cicatrización de heridas apunta a opciones con ingeniería de tejidos, como las matrices descelularizadas, con buenas propiedades de cicatrización y biocompatibilidad. Objetivo: obtener y caracterizar las propiedades de una matriz biológica descelularizada derivada del intestino delgado de animales. Métodos: el intestino delgado porcino se preparó y descelularizó utilizando cuatro métodos diferentes: Triton X-100 (TX-100), dodecil sulfato de sodio (SDS) y desoxicolato de sodio (SDC) para uno o dos ciclos de 6 horas o 24 horas, y ácido peracético para un ciclo de 2 horas. El ADN remanente se cuantificó con Nanodrop y electroforesis. Se realizaron tinciones histológicas y microscopía electrónica de barrido (SEM) para evaluar la estructura e integridad de la superficie. Se realizaron ensayos mecánicos para medir la resistencia de las matrices. Finalmente, se realizaron ensayos de degradabilidad con diferentes soluciones. Resultados: no se encontraron diferencias entre los protocolos de descelularización con respecto al ADN remanente, siendo más eficientes los protocolos de un ciclo de seis horas. Con el menor contenido de ADN remanente y una mejor preservación de la estructura, TX-100 podría considerarse como el mejor protocolo. No se encontraron diferencias estadísticas entre los protocolos y el tejido nativo durante el análisis mecánico. Los ensayos de biodegradabilidad mostraron las propiedades de degradabilidad esperadas de la matriz producida. Conclusión: se lograron resultados prometedores para obtener matrices biológicas descelularizadas que podrían servir como tratamiento para heridas cutáneas complicadas. Se deben realizar más estudios in vitro y moleculares a futuro para caracterizar aún más estas matrices.
publishDate 2023
dc.date.accessioned.none.fl_str_mv 2023-07-15T00:00:00Z
2024-09-05T20:34:53Z
dc.date.available.none.fl_str_mv 2023-07-15T00:00:00Z
2024-09-05T20:34:53Z
dc.date.issued.none.fl_str_mv 2023-07-15
dc.type.spa.fl_str_mv Artículo de revista
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dc.relation.ispartofjournal.spa.fl_str_mv Revista Ciencias Biomédicas
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spelling Ruíz Soto, Juan PabloGalvis Escobar, Sara MaríaRego Londoño, Maria AntoniaMolina Sierra, Juan DavidPineda Molina, Catalina2023-07-15T00:00:00Z2024-09-05T20:34:53Z2023-07-15T00:00:00Z2024-09-05T20:34:53Z2023-07-152215-7840https://hdl.handle.net/11227/1795210.32997/rcb-3023-41352389-7252https://doi.org/10.32997/rcb-3023-4135Introducción: las lesiones cutáneas complicadas se han convertido en un problema de salud mundial, siendo difíciles de tratar debido al limitado proceso de curación del cuerpo. Se han realizado estudios para mejorar los tratamientos tradicionales que tienen muchas desventajas. La investigación en cicatrización de heridas apunta a opciones con ingeniería de tejidos, como las matrices descelularizadas, con buenas propiedades de cicatrización y biocompatibilidad. Objetivo: obtener y caracterizar las propiedades de una matriz biológica descelularizada derivada del intestino delgado de animales. Métodos: el intestino delgado porcino se preparó y descelularizó utilizando cuatro métodos diferentes: Triton X-100 (TX-100), dodecil sulfato de sodio (SDS) y desoxicolato de sodio (SDC) para uno o dos ciclos de 6 horas o 24 horas, y ácido peracético para un ciclo de 2 horas. El ADN remanente se cuantificó con Nanodrop y electroforesis. Se realizaron tinciones histológicas y microscopía electrónica de barrido (SEM) para evaluar la estructura e integridad de la superficie. Se realizaron ensayos mecánicos para medir la resistencia de las matrices. Finalmente, se realizaron ensayos de degradabilidad con diferentes soluciones. Resultados: no se encontraron diferencias entre los protocolos de descelularización con respecto al ADN remanente, siendo más eficientes los protocolos de un ciclo de seis horas. Con el menor contenido de ADN remanente y una mejor preservación de la estructura, TX-100 podría considerarse como el mejor protocolo. No se encontraron diferencias estadísticas entre los protocolos y el tejido nativo durante el análisis mecánico. Los ensayos de biodegradabilidad mostraron las propiedades de degradabilidad esperadas de la matriz producida. Conclusión: se lograron resultados prometedores para obtener matrices biológicas descelularizadas que podrían servir como tratamiento para heridas cutáneas complicadas. Se deben realizar más estudios in vitro y moleculares a futuro para caracterizar aún más estas matrices.Introduction: complicated skin injuries have become a global health problem, being difficult to treat due to the body’s limited healing process. Many studies aim to enhance traditional treatments for skin injuries, which have many disadvantages. Therefore, wound healing research is aiming towards tissue engineering options, such as decellularized matrix, which have shown great healing and biocompatibility competencies. Objectives:  to obtain and characterize the properties of a decellularized biological matrix derived from the small intestine of animals. Methods: porcine small intestine was prepared and decellularized using four different methods: Triton X-100, sodium dodecyl sulfate (SDS) and sodium deoxycholate (SDC) for one or two cycles of 6 hours or 24 hours, and peracetic acid for one cycle of 2 hours. The remaining DNA was quantified with Nanodrop and electrophoresis characterization. Histology stains and Scanning Electron Microscopy (SEM) were performed to assess surface structure and integrity. Resistance assays were conducted to measure mechanical strength. Finally, degradability assays with different buffers were performed. Results: no differences between the decellularization protocols regarding remaining DNA were found, making protocols of one cycle of six hours more efficient. With the least remaining DNA content and better structure perseveration, TX-100 could be considered as the best protocol. No statistically difference between protocols and native tissue were found during the mechanical analysis. Biodegradability assays showed the expected degradability properties of the produced matrix. Conclusions: promising results were achieved to obtain decellularized biological matrices that could serve as a treatment for complicated skin wounds. More in vitro and molecular studies should be carried out in future studies to further characterize these scaffolds.application/pdfengUniversidad de CartagenaRevista Ciencias Biomédicashttps://revistas.unicartagena.edu.co/index.php/cbiomedicas/article/download/4135/3704120310212Karimkhani C, Dellavalle RP, Coffeng LE, Flohr C, Hay RJ, Langan SM, et al. Global Skin Disease Morbidity and Mortality: An Update From the Global Burden of Disease Study 2013. 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Acta Biomater. 2014 Jan;10(1):10.1016/j.actbio.2013.09.006.Juan Pablo Ruíz Soto, Sara María Galvis Escobar, Maria Antonia Rego Londoño, Juan David Molina Sierra, Catalina Pineda Molina - 2023https://creativecommons.org/licenses/by-nc-nd/4.0http://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessEsta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-SinDerivadas 4.0.https://revistas.unicartagena.edu.co/index.php/cbiomedicas/article/view/4135bioactive scaffolddecellularizationporcine small intestineskin woundtissue engineeringmatriz bioactivadescelularizaciónintestino delgado porcinoherida de pielingeniería de tejidosDescelularización y caracterización in vitro de matrices de intestino delgado porcino para el tratamiento de heridas complejasDecellularization and in vitro characterization of porcine small intestine scaffolds for complex wound treatmentsArtículo de revistainfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/version/c_970fb48d4fbd8a85http://purl.org/coar/resource_type/c_6501http://purl.org/coar/resource_type/c_2df8fbb1Textinfo:eu-repo/semantics/articleJournal articlehttp://purl.org/redcol/resource_type/ARTPublicationOREORE.xmltext/xml2866https://repositorio.unicartagena.edu.co/bitstreams/20b8aec4-5eb1-4787-aced-a09bddc4fe26/download0aa6e758926bb2f0d54c029343e85994MD5111227/17952oai:repositorio.unicartagena.edu.co:11227/179522024-09-05 15:34:53.669https://creativecommons.org/licenses/by-nc-nd/4.0Juan Pablo Ruíz Soto, Sara María Galvis Escobar, Maria Antonia Rego Londoño, Juan David Molina Sierra, Catalina Pineda Molina - 2023metadata.onlyhttps://repositorio.unicartagena.edu.coBiblioteca Digital Universidad de Cartagenabdigital@metabiblioteca.com

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