Estudio de captación y bioacumulación de compuestos farmacéuticamente activos en cultivo de Lactuca sativa y evaluación de sus posibles efectos en las características morfológicas de la planta
It has been shown that pharmaceutically active compounds released in agroecosystems are absorbed and accumulated in the edible parts of crop plants. Using a simulated culture under controlled conditions, the present study aimed to evaluate the absorption and bioaccumulation of three common pharmaceu...
- Autores:
-
Carvajal Silva, Angie Valentina
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2021
- Institución:
- Universidad Antonio Nariño
- Repositorio:
- Repositorio UAN
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uan.edu.co:123456789/6380
- Acceso en línea:
- http://repositorio.uan.edu.co/handle/123456789/6380
- Palabra clave:
- Compuestos farmacéuticamente activos
Absorción y bioacumulación
Diclofenaco
Lactuca sativa romana
540
Pharmaceutically active compounds
Absorption and bioaccumulation
Diclofenac
Lactuca sativa
- Rights
- openAccess
- License
- Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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dc.title.es_ES.fl_str_mv |
Estudio de captación y bioacumulación de compuestos farmacéuticamente activos en cultivo de Lactuca sativa y evaluación de sus posibles efectos en las características morfológicas de la planta |
title |
Estudio de captación y bioacumulación de compuestos farmacéuticamente activos en cultivo de Lactuca sativa y evaluación de sus posibles efectos en las características morfológicas de la planta |
spellingShingle |
Estudio de captación y bioacumulación de compuestos farmacéuticamente activos en cultivo de Lactuca sativa y evaluación de sus posibles efectos en las características morfológicas de la planta Compuestos farmacéuticamente activos Absorción y bioacumulación Diclofenaco Lactuca sativa romana 540 Pharmaceutically active compounds Absorption and bioaccumulation Diclofenac Lactuca sativa |
title_short |
Estudio de captación y bioacumulación de compuestos farmacéuticamente activos en cultivo de Lactuca sativa y evaluación de sus posibles efectos en las características morfológicas de la planta |
title_full |
Estudio de captación y bioacumulación de compuestos farmacéuticamente activos en cultivo de Lactuca sativa y evaluación de sus posibles efectos en las características morfológicas de la planta |
title_fullStr |
Estudio de captación y bioacumulación de compuestos farmacéuticamente activos en cultivo de Lactuca sativa y evaluación de sus posibles efectos en las características morfológicas de la planta |
title_full_unstemmed |
Estudio de captación y bioacumulación de compuestos farmacéuticamente activos en cultivo de Lactuca sativa y evaluación de sus posibles efectos en las características morfológicas de la planta |
title_sort |
Estudio de captación y bioacumulación de compuestos farmacéuticamente activos en cultivo de Lactuca sativa y evaluación de sus posibles efectos en las características morfológicas de la planta |
dc.creator.fl_str_mv |
Carvajal Silva, Angie Valentina |
dc.contributor.advisor.spa.fl_str_mv |
Moncayo Lasso, Alejandro |
dc.contributor.author.spa.fl_str_mv |
Carvajal Silva, Angie Valentina |
dc.subject.es_ES.fl_str_mv |
Compuestos farmacéuticamente activos Absorción y bioacumulación Diclofenaco Lactuca sativa romana |
topic |
Compuestos farmacéuticamente activos Absorción y bioacumulación Diclofenaco Lactuca sativa romana 540 Pharmaceutically active compounds Absorption and bioaccumulation Diclofenac Lactuca sativa |
dc.subject.ddc.es_ES.fl_str_mv |
540 |
dc.subject.keyword.es_ES.fl_str_mv |
Pharmaceutically active compounds Absorption and bioaccumulation Diclofenac Lactuca sativa |
description |
It has been shown that pharmaceutically active compounds released in agroecosystems are absorbed and accumulated in the edible parts of crop plants. Using a simulated culture under controlled conditions, the present study aimed to evaluate the absorption and bioaccumulation of three common pharmaceutically active compounds (Diclofenac, Sulfamethoxazole and Losartan) applied individually and in mixture in Lactuca sativa roman crop, and the effects mediated by these compounds on the quality attributes of the leafs plant. Ultrasound assisted solid phase extraction technique and high-performance liquid chromatography was used identified and quantify these compounds in lettuce leaves. Absorption of sulfamethoxazole and losartan was not observed, while diclofenac was detected in concentrations of 0.0372 ng/g. The compounds evaluated and applied at different concentrations significatively affected (lower values) the attributes of the plant such as sugars, humidity, and chlorophyll. On the other hand, it is suggested to continue carrying out studies related with establishment the mobility of pharmaceutical compounds in the plant, their possible metabolization and the probable transformation products of the three pharmaceutical compounds used and of those that may be found in irrigation water samples in crops at field level. |
publishDate |
2021 |
dc.date.issued.spa.fl_str_mv |
2021-11-25 |
dc.date.accessioned.none.fl_str_mv |
2022-04-30T15:01:21Z |
dc.date.available.none.fl_str_mv |
2022-04-30T15:01:21Z |
dc.type.spa.fl_str_mv |
Trabajo de grado (Pregrado y/o Especialización) |
dc.type.coar.spa.fl_str_mv |
http://purl.org/coar/resource_type/c_7a1f |
dc.type.coarversion.none.fl_str_mv |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
format |
http://purl.org/coar/resource_type/c_7a1f |
dc.identifier.uri.none.fl_str_mv |
http://repositorio.uan.edu.co/handle/123456789/6380 |
dc.identifier.bibliographicCitation.spa.fl_str_mv |
Ahmed, M. B. M., Rajapaksha, A. U., Lim, J. E., Vu, N. T., Kim, I. S., Kang, H. M., Lee, S. S., & Ok, Y. S. (2015). Distribution and accumulative pattern of tetracyclines and sulfonamides in edible vegetables of cucumber, tomato, and lettuce. Journal of Agricultural and Food Chemistry, 63(2), 398–405. https://doi.org/10.1021/jf5034637 Balaguera, H. E., Álvarez-Herrera, J. G., & Rodríguez, J. D. (2008). Effect of the water deficit on the transplant of tomato seedlings (Solanum lycopersicum L.). Agronomía Colombiana. Bartha, B., Huber, C., & Schröder, P. (2014). Uptake and metabolism of diclofenac in Typha latifolia-- how plants cope with human pharmaceutical pollution. Plant Science, 227, 12–20. https://doi.org/10.1016/j.plantsci.2014.06.001 Bártíková, H., Podlipná, R., & Skálová, L. (2016). Veterinary drugs in the environment and their toxicity to plants. Chemosphere, 144, 2290–2301. https://doi.org/10.1016/j.chemosphere.2015.10.137 Basnyat, P. (2011). Evaluation of Toxicity of Pharmaceuticals to the Activated Sludge Treatment Plant. Bigott, Y., Chowdhury, S. P., Pérez, S., Montemurro, N., Manasfi, R., & Schröder, P. (2021). Effect of the pharmaceuticals diclofenac and lamotrigine on stress responses and stress gene expression in lettuce (Lactuca sativa) at environmentally relevant concentrations. Journal of Hazardous Materials, 403, 123881. https://doi.org/10.1016/j.jhazmat.2020.123881 Bowne, J. B., Erwin, T. A., Juttner, J., Schnurbusch, T., Langridge, P., Bacic, A., & Roessner, U. (2012). Drought responses of leaf tissues from wheat cultivars of differing drought tolerance at the metabolite level. Molecular Plant, 5(2), 418–429. https://doi.org/10.1093/mp/ssr114 Brausch, J. M., Connors, K. A., Brooks, B. W., & Rand, G. M. (2012). Human pharmaceuticals in the aquatic environment: a review of recent toxicological studies and considerations for toxicity testing. Reviews of Environmental Contamination and Toxicology, 218, 1–99. https://doi.org/10.1007/978-1-4614-3137-4_1 Brunton et al. (2011). As Bases Farmacológicas da Terapêutica de Goodman e Gilman - 13.ed. - Laurence L. Brunton, PhD, Randa Hilal-Dandan, PhD, Björn C. Knollmann, MD, PhD - Google Libros (2018 Artmed Editora, Ed.). Carter, L. J., Williams, M., Böttcher, C., & Kookana, R. S. (2015). Uptake of pharmaceuticals influences plant development and affects nutrient and hormone homeostases. Environmental Science & Technology, 49(20), 12509–12518. https://doi.org/10.1021/acs.est.5b03468 Carvalho, P. N., Basto, M. C. P., Almeida, C. M. R., & Brix, H. (2014). A review of plantpharmaceutical interactions: from uptake and effects in crop plants to phytoremediation in constructed wetlands. Environmental Science and Pollution Research International, 21(20), 11729–11763. https://doi.org/10.1007/s11356-014-2550-3 |
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instname:Universidad Antonio Nariño |
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identifier_str_mv |
Ahmed, M. B. M., Rajapaksha, A. U., Lim, J. E., Vu, N. T., Kim, I. S., Kang, H. M., Lee, S. S., & Ok, Y. S. (2015). Distribution and accumulative pattern of tetracyclines and sulfonamides in edible vegetables of cucumber, tomato, and lettuce. Journal of Agricultural and Food Chemistry, 63(2), 398–405. https://doi.org/10.1021/jf5034637 Balaguera, H. E., Álvarez-Herrera, J. G., & Rodríguez, J. D. (2008). Effect of the water deficit on the transplant of tomato seedlings (Solanum lycopersicum L.). Agronomía Colombiana. Bartha, B., Huber, C., & Schröder, P. (2014). Uptake and metabolism of diclofenac in Typha latifolia-- how plants cope with human pharmaceutical pollution. Plant Science, 227, 12–20. https://doi.org/10.1016/j.plantsci.2014.06.001 Bártíková, H., Podlipná, R., & Skálová, L. (2016). Veterinary drugs in the environment and their toxicity to plants. Chemosphere, 144, 2290–2301. https://doi.org/10.1016/j.chemosphere.2015.10.137 Basnyat, P. (2011). Evaluation of Toxicity of Pharmaceuticals to the Activated Sludge Treatment Plant. Bigott, Y., Chowdhury, S. P., Pérez, S., Montemurro, N., Manasfi, R., & Schröder, P. (2021). Effect of the pharmaceuticals diclofenac and lamotrigine on stress responses and stress gene expression in lettuce (Lactuca sativa) at environmentally relevant concentrations. Journal of Hazardous Materials, 403, 123881. https://doi.org/10.1016/j.jhazmat.2020.123881 Bowne, J. B., Erwin, T. A., Juttner, J., Schnurbusch, T., Langridge, P., Bacic, A., & Roessner, U. (2012). Drought responses of leaf tissues from wheat cultivars of differing drought tolerance at the metabolite level. Molecular Plant, 5(2), 418–429. https://doi.org/10.1093/mp/ssr114 Brausch, J. M., Connors, K. A., Brooks, B. W., & Rand, G. M. (2012). Human pharmaceuticals in the aquatic environment: a review of recent toxicological studies and considerations for toxicity testing. Reviews of Environmental Contamination and Toxicology, 218, 1–99. https://doi.org/10.1007/978-1-4614-3137-4_1 Brunton et al. (2011). As Bases Farmacológicas da Terapêutica de Goodman e Gilman - 13.ed. - Laurence L. Brunton, PhD, Randa Hilal-Dandan, PhD, Björn C. Knollmann, MD, PhD - Google Libros (2018 Artmed Editora, Ed.). Carter, L. J., Williams, M., Böttcher, C., & Kookana, R. S. (2015). Uptake of pharmaceuticals influences plant development and affects nutrient and hormone homeostases. Environmental Science & Technology, 49(20), 12509–12518. https://doi.org/10.1021/acs.est.5b03468 Carvalho, P. N., Basto, M. C. P., Almeida, C. M. R., & Brix, H. (2014). A review of plantpharmaceutical interactions: from uptake and effects in crop plants to phytoremediation in constructed wetlands. Environmental Science and Pollution Research International, 21(20), 11729–11763. https://doi.org/10.1007/s11356-014-2550-3 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ |
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dc.publisher.spa.fl_str_mv |
Universidad Antonio Nariño |
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Bioquímica |
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Facultad de Ciencias |
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Bogotá - Circunvalar |
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Universidad Antonio Nariño |
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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Moncayo Lasso, AlejandroCarvajal Silva, Angie Valentina118217140112022-04-30T15:01:21Z2022-04-30T15:01:21Z2021-11-25http://repositorio.uan.edu.co/handle/123456789/6380Ahmed, M. B. M., Rajapaksha, A. U., Lim, J. E., Vu, N. T., Kim, I. S., Kang, H. M., Lee, S. S., & Ok, Y. S. (2015). Distribution and accumulative pattern of tetracyclines and sulfonamides in edible vegetables of cucumber, tomato, and lettuce. Journal of Agricultural and Food Chemistry, 63(2), 398–405. https://doi.org/10.1021/jf5034637Balaguera, H. E., Álvarez-Herrera, J. G., & Rodríguez, J. D. (2008). Effect of the water deficit on the transplant of tomato seedlings (Solanum lycopersicum L.). Agronomía Colombiana.Bartha, B., Huber, C., & Schröder, P. (2014). Uptake and metabolism of diclofenac in Typha latifolia-- how plants cope with human pharmaceutical pollution. Plant Science, 227, 12–20. https://doi.org/10.1016/j.plantsci.2014.06.001Bártíková, H., Podlipná, R., & Skálová, L. (2016). Veterinary drugs in the environment and their toxicity to plants. Chemosphere, 144, 2290–2301. https://doi.org/10.1016/j.chemosphere.2015.10.137 Basnyat, P. (2011). Evaluation of Toxicity of Pharmaceuticals to the Activated Sludge Treatment Plant.Bigott, Y., Chowdhury, S. P., Pérez, S., Montemurro, N., Manasfi, R., & Schröder, P. (2021). Effect of the pharmaceuticals diclofenac and lamotrigine on stress responses and stress gene expression in lettuce (Lactuca sativa) at environmentally relevant concentrations. Journal of Hazardous Materials, 403, 123881. https://doi.org/10.1016/j.jhazmat.2020.123881Bowne, J. B., Erwin, T. A., Juttner, J., Schnurbusch, T., Langridge, P., Bacic, A., & Roessner, U. (2012). Drought responses of leaf tissues from wheat cultivars of differing drought tolerance at the metabolite level. Molecular Plant, 5(2), 418–429. https://doi.org/10.1093/mp/ssr114Brausch, J. M., Connors, K. A., Brooks, B. W., & Rand, G. M. (2012). Human pharmaceuticals in the aquatic environment: a review of recent toxicological studies and considerations for toxicity testing. Reviews of Environmental Contamination and Toxicology, 218, 1–99. https://doi.org/10.1007/978-1-4614-3137-4_1Brunton et al. (2011). As Bases Farmacológicas da Terapêutica de Goodman e Gilman - 13.ed. - Laurence L. Brunton, PhD, Randa Hilal-Dandan, PhD, Björn C. Knollmann, MD, PhD - Google Libros (2018 Artmed Editora, Ed.).Carter, L. J., Williams, M., Böttcher, C., & Kookana, R. S. (2015). Uptake of pharmaceuticals influences plant development and affects nutrient and hormone homeostases. Environmental Science & Technology, 49(20), 12509–12518. https://doi.org/10.1021/acs.est.5b03468Carvalho, P. N., Basto, M. C. P., Almeida, C. M. R., & Brix, H. (2014). A review of plantpharmaceutical interactions: from uptake and effects in crop plants to phytoremediation in constructed wetlands. Environmental Science and Pollution Research International, 21(20), 11729–11763. https://doi.org/10.1007/s11356-014-2550-3instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/It has been shown that pharmaceutically active compounds released in agroecosystems are absorbed and accumulated in the edible parts of crop plants. Using a simulated culture under controlled conditions, the present study aimed to evaluate the absorption and bioaccumulation of three common pharmaceutically active compounds (Diclofenac, Sulfamethoxazole and Losartan) applied individually and in mixture in Lactuca sativa roman crop, and the effects mediated by these compounds on the quality attributes of the leafs plant. Ultrasound assisted solid phase extraction technique and high-performance liquid chromatography was used identified and quantify these compounds in lettuce leaves. Absorption of sulfamethoxazole and losartan was not observed, while diclofenac was detected in concentrations of 0.0372 ng/g. The compounds evaluated and applied at different concentrations significatively affected (lower values) the attributes of the plant such as sugars, humidity, and chlorophyll. On the other hand, it is suggested to continue carrying out studies related with establishment the mobility of pharmaceutical compounds in the plant, their possible metabolization and the probable transformation products of the three pharmaceutical compounds used and of those that may be found in irrigation water samples in crops at field level.Se ha comprobado que los compuestos farmacéuticamente activos liberados en los agroecosistemas son absorbidos y acumulados en las partes comestibles de las plantas de cultivo. Mediante el empleo de un cultivo simulado en condiciones controladas, el presente estudio tuvo como objetivo evaluar la absorción y bioacumulación de tres compuestos farmacéuticamente activos comunes (Diclofenaco, Sulfametoxazol y Losartán) aplicados individualmente y en mezcla en el agua de riego del cultivo de Lactuca sativa romana, así como los efectos mediados por estos compuestos en los atributos de la calidad de las plantas. Para esto, se emplearon las técnicas de extracción en fase sólida asistida por ultrasonido en conjunto para la cromatografía líquida de alta eficiencia para su identificación en las hojas de la planta. Se observó que los compuestos sulfametoxazol y losartán no son absorbidos en las hojas de lechuga, mientras que el diclofenaco se detectó en concentraciones de 0.0372 ng/g. Se evidenció que los compuestos estudiados aplicados en diferentes niveles de concentraciones afectaron significativamente (disminuyeron sus valores) los atributos de la planta tales como niveles de azúcares, porcentaje de humedad y clorofila. Por otro lado, se sugiere seguir realizando estudios que permitan establecer la movilidad de los fármacos en la planta, su posible metabolización y los probables productos de transformación de los tres compuestos farmacéuticos empleados y de los que puedan encontrarse en las muestras de agua de riego en cultivos a nivel campo.Bioquímico(a)PregradoPresencialInvestigaciónspaUniversidad Antonio NariñoBioquímicaFacultad de CienciasBogotá - CircunvalarCompuestos farmacéuticamente activosAbsorción y bioacumulaciónDiclofenacoLactuca sativa romana540Pharmaceutically active compoundsAbsorption and bioaccumulationDiclofenacLactuca sativaEstudio de captación y bioacumulación de compuestos farmacéuticamente activos en cultivo de Lactuca sativa y evaluación de sus posibles efectos en las características morfológicas de la plantaTrabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85EspecializadaORIGINAL2021_AngieValentinaCarvajalSilva_Acta.pdf2021_AngieValentinaCarvajalSilva_Acta.pdfActa de sustentaciónapplication/pdf496167https://repositorio.uan.edu.co/bitstreams/7f54fd95-098a-4557-9eae-868640611536/download2c99e36705c2cb6b63421134b9b454c9MD512021_AngieValentinaCarvajalSilva_Autorización.pdf2021_AngieValentinaCarvajalSilva_Autorización.pdfFormato de autorización de autoresapplication/pdf669616https://repositorio.uan.edu.co/bitstreams/a80b2b16-f1af-4e6d-9977-e7d148d52ce8/download7578178c25b62ad7313b051be7f67214MD522021_AngieValentinaCarvajalSilva.pdf2021_AngieValentinaCarvajalSilva.pdfTrabajo de gradoapplication/pdf3485702https://repositorio.uan.edu.co/bitstreams/79b76e5c-f510-46ac-8ba9-a1347c45ef9d/download1025b741e1150597b42aaf5f9df971d4MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.uan.edu.co/bitstreams/2e90979f-cd94-4847-a3d2-444fc1f5024c/download9868ccc48a14c8d591352b6eaf7f6239MD54123456789/6380oai:repositorio.uan.edu.co:123456789/63802024-10-09 23:11:43.043https://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertorestrictedhttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co |