Cambios en las proteínas asociadas a estrés oxidativo de Apis mellifera expuestas al insecticida sistémico Imidacloprid.

The increase in agricultural crops has brought with it the increase in the use of insecticides, however, its application has collaterally impacted pollinating insects. Insecticides induce oxidative stress in bees, but the reason why it occurs has not yet been fully elucidated, which is why this stud...

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Autores:: Galindo Morales, Fredy Gonzalo

Tipo de recurso:: Trabajo de grado de pregrado

Fecha de publicación:: 2023

Institución:: Universidad Antonio Nariño

Repositorio:: Repositorio UAN

Idioma:: spa

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dc.title.es_ES.fl_str_mv	Cambios en las proteínas asociadas a estrés oxidativo de Apis mellifera expuestas al insecticida sistémico Imidacloprid.
title	Cambios en las proteínas asociadas a estrés oxidativo de Apis mellifera expuestas al insecticida sistémico Imidacloprid.
spellingShingle	Cambios en las proteínas asociadas a estrés oxidativo de Apis mellifera expuestas al insecticida sistémico Imidacloprid. Abeja, imidacloprid, pesticidas, proteómica, acoplamiento molecular. imidacloprid pesticidas proteómica acoplamiento molecular 574 38.23 G158c Bee imidacloprid proteomics molecular docking pesticides
title_short	Cambios en las proteínas asociadas a estrés oxidativo de Apis mellifera expuestas al insecticida sistémico Imidacloprid.
title_full	Cambios en las proteínas asociadas a estrés oxidativo de Apis mellifera expuestas al insecticida sistémico Imidacloprid.
title_fullStr	Cambios en las proteínas asociadas a estrés oxidativo de Apis mellifera expuestas al insecticida sistémico Imidacloprid.
title_full_unstemmed	Cambios en las proteínas asociadas a estrés oxidativo de Apis mellifera expuestas al insecticida sistémico Imidacloprid.
title_sort	Cambios en las proteínas asociadas a estrés oxidativo de Apis mellifera expuestas al insecticida sistémico Imidacloprid.
dc.creator.fl_str_mv	Galindo Morales, Fredy Gonzalo
dc.contributor.advisor.spa.fl_str_mv	Arenas Suarez, Nelson Enrique
dc.contributor.author.spa.fl_str_mv	Galindo Morales, Fredy Gonzalo
dc.subject.es_ES.fl_str_mv	Abeja, imidacloprid, pesticidas, proteómica, acoplamiento molecular. imidacloprid pesticidas proteómica acoplamiento molecular
topic	Abeja, imidacloprid, pesticidas, proteómica, acoplamiento molecular. imidacloprid pesticidas proteómica acoplamiento molecular 574 38.23 G158c Bee imidacloprid proteomics molecular docking pesticides
dc.subject.ddc.es_ES.fl_str_mv	574 38.23 G158c
dc.subject.keyword.es_ES.fl_str_mv	Bee imidacloprid proteomics molecular docking pesticides
description	The increase in agricultural crops has brought with it the increase in the use of insecticides, however, its application has collaterally impacted pollinating insects. Insecticides induce oxidative stress in bees, but the reason why it occurs has not yet been fully elucidated, which is why this study sought to describe the interactions between the insecticide imidacloprid with catalase, glutathione peroxidase and superoxide dismutase using molecular docking, as well as evaluating the expression changes of proteins related to oxidative stress of protein extracts from A. mellifera.
publishDate	2023
dc.date.accessioned.none.fl_str_mv	2023-07-31T20:22:03Z
dc.date.available.none.fl_str_mv	2023-07-31T20:22:03Z
dc.date.issued.spa.fl_str_mv	2023-05-24
dc.type.spa.fl_str_mv	Trabajo de grado (Pregrado y/o Especialización)
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dc.identifier.uri.none.fl_str_mv	http://repositorio.uan.edu.co/handle/123456789/8343
dc.identifier.bibliographicCitation.spa.fl_str_mv	Aguiar, J. M. R. B. V., Nocelli, R. C. F., Giurfa, M., & Nascimento, F. S. (2023). Neonicotinoid effects on tropical bees: Imidacloprid impairs innate appetitive responsiveness, learning and memory in the stingless bee Melipona quadrifasciata. Science of The Total Environment, 877,162859. https://doi.org/10.1016/J.SCITOTENV.2023.162859 Aslam, B., Basit, M., Nisar, M. A., Khurshid, M., & Rasool, M. H. (2017). Proteomics: Technologies and Their Applications. Journal of Chromatographic Science, 55(2), 182–196. https://doi.org/10.1093/CHROMSCI/BMW167 Balieira, K. V. B., Mazzo, M., Bizerra, P. F. V., Guimarães, A. R. de J. S., Nicodemo, D., & Mingatto, F. E. (2018). Imidacloprid-induced oxidative stress in honey bees and the antioxidant action of caffeine. Apidologie, 49(5), 562–572. https://doi.org/10.1007/S13592-018-0583-1/TABLES/3 Blum, M., Chang, H. Y., Chuguransky, S., Grego, T., Kandasaamy, S., Mitchell, A., Nuka, G., Paysan-Lafosse, T., Qureshi, M., Raj, S., Richardson, L., Salazar, G. A., Williams, L., Bork, P., Bridge, A., Gough, J., Haft, D. H., Letunic, I., Marchler-Bauer, A., … Finn, R. D. (2021). The InterPro protein families and domains database: 20 years on. Nucleic Acids Research, 49(D1), D344–D354. https://doi.org/10.1093/NAR/GKAA977 Boily, M., Sarrasin, B., DeBlois, C., Aras, P., & Chagnon, M. (2013). Acetylcholinesterase in honey bees (Apis mellifera) exposed to neonicotinoids, atrazine and glyphosate: laboratory and field experiments. Environmental Science and Pollution Research International, 20(8), 5603–5614.https://doi.org/10.1007/S11356-013-1568-2 Brandt, A., Gorenflo, A., Siede, R., Meixner, M., & Büchler, R. (2016). The neonicotinoids thiacloprid, imidacloprid, and clothianidin affect the immunocompetence of honey bees (Apis mellifera L.). Journal of Insect Physiology, 86, 40–47. https://doi.org/10.1016/J.JINSPHYS.2016.01.001 Buszewski, B., Bukowska, M., Ligor, M., & Staneczko-Baranowska, I. (2019). A holistic study of neonicotinoids neuroactive insecticides—properties, applications, occurrence, and analysis. Environmental Science and Pollution Research, 26(34), 34723–34740. https://doi.org/10.1007/S11356-019-06114-W/TABLES/3 Casida, J. E., & Durkin, K. A. (2013). Neuroactive insecticides: Targets, selectivity, resistance, and secondary effects. Annual Review of Entomology, 58, 99–117. https://doi.org/10.1146/annurevento-120811-153645 Castellano, I., Cecere, F., De Vendittis, A., Cotugno, R., Chambery, A., Di Maro, A., Michniewicz, A., Parlato, G., Masullo, M., Avvedimento, E. V., De Vendittis, E., & Ruocco, M. R. (2009). Rat mitochondrial manganese superoxide dismutase: Amino acid positions involved in covalent modifications, activity, and heat stability. Biopolymers, 91(12), 1215–1226. https://doi.org/10.1002/BIP.21208 Catae, A. F., Roat, T. C., Pratavieira, M., Silva Menegasso, A. R. da, Palma, M. S., & Malaspina, O. (2017). Exposure to a sublethal concentration of imidacloprid and the side effects on target and nontarget organs of Apis mellifera (Hymenoptera, Apidae). Ecotoxicology 2017 27:2, 27(2), 109–121. https://doi.org/10.1007/S10646-017-1874-4
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identifier_str_mv	Aguiar, J. M. R. B. V., Nocelli, R. C. F., Giurfa, M., & Nascimento, F. S. (2023). Neonicotinoid effects on tropical bees: Imidacloprid impairs innate appetitive responsiveness, learning and memory in the stingless bee Melipona quadrifasciata. Science of The Total Environment, 877,162859. https://doi.org/10.1016/J.SCITOTENV.2023.162859 Aslam, B., Basit, M., Nisar, M. A., Khurshid, M., & Rasool, M. H. (2017). Proteomics: Technologies and Their Applications. Journal of Chromatographic Science, 55(2), 182–196. https://doi.org/10.1093/CHROMSCI/BMW167 Balieira, K. V. B., Mazzo, M., Bizerra, P. F. V., Guimarães, A. R. de J. S., Nicodemo, D., & Mingatto, F. E. (2018). Imidacloprid-induced oxidative stress in honey bees and the antioxidant action of caffeine. Apidologie, 49(5), 562–572. https://doi.org/10.1007/S13592-018-0583-1/TABLES/3 Blum, M., Chang, H. Y., Chuguransky, S., Grego, T., Kandasaamy, S., Mitchell, A., Nuka, G., Paysan-Lafosse, T., Qureshi, M., Raj, S., Richardson, L., Salazar, G. A., Williams, L., Bork, P., Bridge, A., Gough, J., Haft, D. H., Letunic, I., Marchler-Bauer, A., … Finn, R. D. (2021). The InterPro protein families and domains database: 20 years on. Nucleic Acids Research, 49(D1), D344–D354. https://doi.org/10.1093/NAR/GKAA977 Boily, M., Sarrasin, B., DeBlois, C., Aras, P., & Chagnon, M. (2013). Acetylcholinesterase in honey bees (Apis mellifera) exposed to neonicotinoids, atrazine and glyphosate: laboratory and field experiments. Environmental Science and Pollution Research International, 20(8), 5603–5614.https://doi.org/10.1007/S11356-013-1568-2 Brandt, A., Gorenflo, A., Siede, R., Meixner, M., & Büchler, R. (2016). The neonicotinoids thiacloprid, imidacloprid, and clothianidin affect the immunocompetence of honey bees (Apis mellifera L.). Journal of Insect Physiology, 86, 40–47. https://doi.org/10.1016/J.JINSPHYS.2016.01.001 Buszewski, B., Bukowska, M., Ligor, M., & Staneczko-Baranowska, I. (2019). A holistic study of neonicotinoids neuroactive insecticides—properties, applications, occurrence, and analysis. Environmental Science and Pollution Research, 26(34), 34723–34740. https://doi.org/10.1007/S11356-019-06114-W/TABLES/3 Casida, J. E., & Durkin, K. A. (2013). Neuroactive insecticides: Targets, selectivity, resistance, and secondary effects. Annual Review of Entomology, 58, 99–117. https://doi.org/10.1146/annurevento-120811-153645 Castellano, I., Cecere, F., De Vendittis, A., Cotugno, R., Chambery, A., Di Maro, A., Michniewicz, A., Parlato, G., Masullo, M., Avvedimento, E. V., De Vendittis, E., & Ruocco, M. R. (2009). Rat mitochondrial manganese superoxide dismutase: Amino acid positions involved in covalent modifications, activity, and heat stability. Biopolymers, 91(12), 1215–1226. https://doi.org/10.1002/BIP.21208 Catae, A. F., Roat, T. C., Pratavieira, M., Silva Menegasso, A. R. da, Palma, M. S., & Malaspina, O. (2017). Exposure to a sublethal concentration of imidacloprid and the side effects on target and nontarget organs of Apis mellifera (Hymenoptera, Apidae). Ecotoxicology 2017 27:2, 27(2), 109–121. https://doi.org/10.1007/S10646-017-1874-4 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/
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dc.publisher.spa.fl_str_mv	Universidad Antonio Nariño
dc.publisher.program.spa.fl_str_mv	Bioquímica
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.campus.spa.fl_str_mv	Bogotá - Circunvalar
institution	Universidad Antonio Nariño
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spelling	Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Arenas Suarez, Nelson EnriqueGalindo Morales, Fredy Gonzalo118218222962023-07-31T20:22:03Z2023-07-31T20:22:03Z2023-05-24http://repositorio.uan.edu.co/handle/123456789/8343Aguiar, J. M. R. B. V., Nocelli, R. C. F., Giurfa, M., & Nascimento, F. S. (2023). Neonicotinoid effects on tropical bees: Imidacloprid impairs innate appetitive responsiveness, learning and memory in the stingless bee Melipona quadrifasciata. Science of The Total Environment, 877,162859. https://doi.org/10.1016/J.SCITOTENV.2023.162859Aslam, B., Basit, M., Nisar, M. A., Khurshid, M., & Rasool, M. H. (2017). Proteomics: Technologies and Their Applications. Journal of Chromatographic Science, 55(2), 182–196. https://doi.org/10.1093/CHROMSCI/BMW167Balieira, K. V. B., Mazzo, M., Bizerra, P. F. V., Guimarães, A. R. de J. S., Nicodemo, D., & Mingatto, F. E. (2018). Imidacloprid-induced oxidative stress in honey bees and the antioxidant action of caffeine. Apidologie, 49(5), 562–572. https://doi.org/10.1007/S13592-018-0583-1/TABLES/3Blum, M., Chang, H. Y., Chuguransky, S., Grego, T., Kandasaamy, S., Mitchell, A., Nuka, G., Paysan-Lafosse, T., Qureshi, M., Raj, S., Richardson, L., Salazar, G. A., Williams, L., Bork, P., Bridge, A., Gough, J., Haft, D. H., Letunic, I., Marchler-Bauer, A., … Finn, R. D. (2021). The InterPro protein families and domains database: 20 years on. Nucleic Acids Research, 49(D1), D344–D354. https://doi.org/10.1093/NAR/GKAA977Boily, M., Sarrasin, B., DeBlois, C., Aras, P., & Chagnon, M. (2013). Acetylcholinesterase in honey bees (Apis mellifera) exposed to neonicotinoids, atrazine and glyphosate: laboratory and field experiments. Environmental Science and Pollution Research International, 20(8), 5603–5614.https://doi.org/10.1007/S11356-013-1568-2Brandt, A., Gorenflo, A., Siede, R., Meixner, M., & Büchler, R. (2016). The neonicotinoids thiacloprid, imidacloprid, and clothianidin affect the immunocompetence of honey bees (Apis mellifera L.). Journal of Insect Physiology, 86, 40–47. https://doi.org/10.1016/J.JINSPHYS.2016.01.001Buszewski, B., Bukowska, M., Ligor, M., & Staneczko-Baranowska, I. (2019). A holistic study of neonicotinoids neuroactive insecticides—properties, applications, occurrence, and analysis. Environmental Science and Pollution Research, 26(34), 34723–34740. https://doi.org/10.1007/S11356-019-06114-W/TABLES/3Casida, J. E., & Durkin, K. A. (2013). Neuroactive insecticides: Targets, selectivity, resistance, and secondary effects. Annual Review of Entomology, 58, 99–117. https://doi.org/10.1146/annurevento-120811-153645Castellano, I., Cecere, F., De Vendittis, A., Cotugno, R., Chambery, A., Di Maro, A., Michniewicz, A., Parlato, G., Masullo, M., Avvedimento, E. V., De Vendittis, E., & Ruocco, M. R. (2009). Rat mitochondrial manganese superoxide dismutase: Amino acid positions involved in covalent modifications, activity, and heat stability. Biopolymers, 91(12), 1215–1226. https://doi.org/10.1002/BIP.21208Catae, A. F., Roat, T. C., Pratavieira, M., Silva Menegasso, A. R. da, Palma, M. S., & Malaspina, O. (2017). Exposure to a sublethal concentration of imidacloprid and the side effects on target and nontarget organs of Apis mellifera (Hymenoptera, Apidae). Ecotoxicology 2017 27:2, 27(2), 109–121. https://doi.org/10.1007/S10646-017-1874-4instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/The increase in agricultural crops has brought with it the increase in the use of insecticides, however, its application has collaterally impacted pollinating insects. Insecticides induce oxidative stress in bees, but the reason why it occurs has not yet been fully elucidated, which is why this study sought to describe the interactions between the insecticide imidacloprid with catalase, glutathione peroxidase and superoxide dismutase using molecular docking, as well as evaluating the expression changes of proteins related to oxidative stress of protein extracts from A. mellifera.El incremento de los cultivos agrícolas ha traído consigo el aumento del uso de insecticidas, sin embargo, su aplicación ha impactado colateralmente a insectos polinizadores. Los insecticidas inducen estrés oxidativo en abejas, mas, la razón por la cual se presenta, aún no se dilucida completamente, es por ello, que en este estudio se buscó describir las interacciones entre el insecticida imidacloprid con catalasa, glutatión peroxidasa y superóxido dismutasa mediante acoplamiento molecular, así como evaluar los cambios de expresión de las proteínas relacionadas con estrés oxidativo de extractos proteicos de A. mellifera.Bioquímico(a)PregradoPresencialInvestigaciónspaUniversidad Antonio NariñoBioquímicaFacultad de CienciasBogotá - CircunvalarAbeja, imidacloprid, pesticidas, proteómica, acoplamiento molecular.imidaclopridpesticidasproteómicaacoplamiento molecular57438.23 G158cBeeimidaclopridproteomicsmolecular dockingpesticidesCambios en las proteínas asociadas a estrés oxidativo de Apis mellifera expuestas al insecticida sistémico Imidacloprid.Trabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85GeneralORIGINAL2023_FredyGonzaloGalindoMorales.pdf2023_FredyGonzaloGalindoMorales.pdfapplication/pdf1495722https://repositorio.uan.edu.co/bitstreams/2b21d203-274c-448e-a203-650131c039a0/downloada9007bf783c9d202f7f5843885699ab5MD512023_FredyGonzaloGalindoMorales_Acta .pdf2023_FredyGonzaloGalindoMorales_Acta .pdfapplication/pdf1581009https://repositorio.uan.edu.co/bitstreams/86e8802f-820d-43da-9cd6-447f7ec72dc5/downloadd8c8a6b8f0bf56e7d6380de1701dec6aMD532023_FredyGonzaloGalindoMorales_Autorización.pdf2023_FredyGonzaloGalindoMorales_Autorización.pdfapplication/pdf330876https://repositorio.uan.edu.co/bitstreams/b947cdfe-334b-44ab-887e-f75d7f53e953/download85c5c852443c7d16fad2c9f7d10541c1MD562023_FredyGonzaloGalindoMorales_Autorización.pdf2023_FredyGonzaloGalindoMorales_Autorización.pdfapplication/pdf330876https://repositorio.uan.edu.co/bitstreams/bb445d83-3bd7-4ba1-8a9c-6294b4a8faf9/download85c5c852443c7d16fad2c9f7d10541c1MD57CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.uan.edu.co/bitstreams/aa337258-7da6-41f9-a04f-1fe16959a2db/download9868ccc48a14c8d591352b6eaf7f6239MD58123456789/8343oai:repositorio.uan.edu.co:123456789/83432024-10-09 23:28:42.626https://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertoopen.accesshttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co

Cambios en las proteínas asociadas a estrés oxidativo de Apis mellifera expuestas al insecticida sistémico Imidacloprid.

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