Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293.
The NMDA (N-methyl-D-aspartate) receptor plays a crucial role in synaptic function and neuronal plasticity, but its hyperactivation can lead to excitotoxicity, a phenomenon implicated in several neurodegenerative diseases. In this context, the use of peptides derived from animal toxins has emerged a...
- Autores:
-
Duque Guevara, Stephany Valentina
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2023
- Institución:
- Universidad Antonio Nariño
- Repositorio:
- Repositorio UAN
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uan.edu.co:123456789/8345
- Acceso en línea:
- http://repositorio.uan.edu.co/handle/123456789/8345
- Palabra clave:
- Bioquimica
574
38.23 D946e
Biochemistry
- Rights
- openAccess
- License
- Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293. |
| title |
Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293. |
| spellingShingle |
Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293. Bioquimica 574 38.23 D946e Biochemistry |
| title_short |
Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293. |
| title_full |
Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293. |
| title_fullStr |
Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293. |
| title_full_unstemmed |
Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293. |
| title_sort |
Evaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293. |
| dc.creator.fl_str_mv |
Duque Guevara, Stephany Valentina |
| dc.contributor.advisor.spa.fl_str_mv |
Reyes Guzmán, Edwin Alfredo Cuervo Soto, Laura Inés |
| dc.contributor.author.spa.fl_str_mv |
Duque Guevara, Stephany Valentina |
| dc.subject.es_ES.fl_str_mv |
Bioquimica |
| topic |
Bioquimica 574 38.23 D946e Biochemistry |
| dc.subject.ddc.es_ES.fl_str_mv |
574 38.23 D946e |
| dc.subject.keyword.es_ES.fl_str_mv |
Biochemistry |
| description |
The NMDA (N-methyl-D-aspartate) receptor plays a crucial role in synaptic function and neuronal plasticity, but its hyperactivation can lead to excitotoxicity, a phenomenon implicated in several neurodegenerative diseases. In this context, the use of peptides derived from animal toxins has emerged as a promising strategy to selectively modulate NMDA receptor activity and counteract excitotoxicity. These peptides, from diverse sources such as snakes and spiders, act as specific ligands of the NMDA receptor and can function as agonists, antagonists or allosteric modulators, interacting with different binding sites on the receptor. |
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2023 |
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2023-07-31T21:26:58Z |
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2023-07-31T21:26:58Z |
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2023-05-29 |
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Trabajo de grado (Pregrado y/o Especialización) |
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http://repositorio.uan.edu.co/handle/123456789/8345 |
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Amico-Ruvio, S. A., Paganelli, M. A., Myers, J. M., & Popescu, G. K. (2012). Ifenprodil effects on GluN2B-containing glutamate receptors. Molecular Pharmacology, 82(6), 1074-1081. https://doi.org/10.1124/mol.112.078998. Asztély F, Gustafsson B. Ionotropic glutamate receptors: Their possible role in the expression of hippocampal synaptic plasticity. Mol Neurobiol. 1996;12(1):1–11. Aizenman E, Frosch MP, Lipton SA. Responses mediated by excitatory amino acid receptors in solitary retinal ganglion cells from rat. J Physiol. 1988;396(1):75–91. Andreou LV. Isolation of plasmid DNA from bacteria. Methods Enzymol. 2013;529:135-42. doi: 10.1016/B978-0-12-418687-3.00010-0. PMID: 24011041. Albensi, B. C. (2007). The NMDA receptor/ion channel complex: a drug target for modulating synaptic plasticity and excitotoxicity. Current pharmaceutical design, 13(31), 3185-3194. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., & Struhl, K. (Eds.). (2005). Current Protocols in Molecular Biology. John Wiley & Sons. Baez, S. D. L. C., García del Barco, D., Hardy-Sosa, A., Guillen Nieto, G., Bringas-Vega, M. L., Llibre-Guerra, J. J., & Valdes-Sosa, P. (2021). Scalable Bio marker combinations for early stroke diagnosis: A systematic review. Frontiers in neurology, 12, 638693. Beaudoin, G. M., Lee, S. H., Singh, D., Yuan, Y., Ng, Y. G., Reichardt, L. F., & Arikkath, J. (2012). Culturing pyramidal neurons from the early postnatal mouse hippocampus and cortex. Nature protocols, 7(9), 1741-1754. Beesley, S., Sullenberger, T., Pilli, J., Abbasi, S., Gunjan, A., & Kumar, S. S. (2019). Colocalization of distinct NMDA receptor subtypes at excitatory synapses in the entorhinal cortex. Journal of Neurophysiology, 121(1), 238-254. https://doi.org/10.1152/jn.00468.2018 Bermúdez Urrego, Y. S. (2022). Evaluation of the effect of the Pb53a a synthetic peptide obtained from toxins of the spider Phoneutria boliviensis on the cell viability of the HEK293 line. Bermudez, S and Reyes-Guzman EA. (2020). Tesis de pregrado. Evaluation of the effect of the Pb53a, a synthetic peptide obtained from toxins of the spider Phoneutria boliviensis on the cell viability of the HEK-293 line |
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Amico-Ruvio, S. A., Paganelli, M. A., Myers, J. M., & Popescu, G. K. (2012). Ifenprodil effects on GluN2B-containing glutamate receptors. Molecular Pharmacology, 82(6), 1074-1081. https://doi.org/10.1124/mol.112.078998. Asztély F, Gustafsson B. Ionotropic glutamate receptors: Their possible role in the expression of hippocampal synaptic plasticity. Mol Neurobiol. 1996;12(1):1–11. Aizenman E, Frosch MP, Lipton SA. Responses mediated by excitatory amino acid receptors in solitary retinal ganglion cells from rat. J Physiol. 1988;396(1):75–91. Andreou LV. Isolation of plasmid DNA from bacteria. Methods Enzymol. 2013;529:135-42. doi: 10.1016/B978-0-12-418687-3.00010-0. PMID: 24011041. Albensi, B. C. (2007). The NMDA receptor/ion channel complex: a drug target for modulating synaptic plasticity and excitotoxicity. Current pharmaceutical design, 13(31), 3185-3194. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., & Struhl, K. (Eds.). (2005). Current Protocols in Molecular Biology. John Wiley & Sons. Baez, S. D. L. C., García del Barco, D., Hardy-Sosa, A., Guillen Nieto, G., Bringas-Vega, M. L., Llibre-Guerra, J. J., & Valdes-Sosa, P. (2021). Scalable Bio marker combinations for early stroke diagnosis: A systematic review. Frontiers in neurology, 12, 638693. Beaudoin, G. M., Lee, S. H., Singh, D., Yuan, Y., Ng, Y. G., Reichardt, L. F., & Arikkath, J. (2012). Culturing pyramidal neurons from the early postnatal mouse hippocampus and cortex. Nature protocols, 7(9), 1741-1754. Beesley, S., Sullenberger, T., Pilli, J., Abbasi, S., Gunjan, A., & Kumar, S. S. (2019). Colocalization of distinct NMDA receptor subtypes at excitatory synapses in the entorhinal cortex. Journal of Neurophysiology, 121(1), 238-254. https://doi.org/10.1152/jn.00468.2018 Bermúdez Urrego, Y. S. (2022). Evaluation of the effect of the Pb53a a synthetic peptide obtained from toxins of the spider Phoneutria boliviensis on the cell viability of the HEK293 line. Bermudez, S and Reyes-Guzman EA. (2020). Tesis de pregrado. Evaluation of the effect of the Pb53a, a synthetic peptide obtained from toxins of the spider Phoneutria boliviensis on the cell viability of the HEK-293 line instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ |
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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Reyes Guzmán, Edwin AlfredoCuervo Soto, Laura InésDuque Guevara, Stephany Valentina118217277562023-07-31T21:26:58Z2023-07-31T21:26:58Z2023-05-29http://repositorio.uan.edu.co/handle/123456789/8345Amico-Ruvio, S. A., Paganelli, M. A., Myers, J. M., & Popescu, G. K. (2012). Ifenprodil effects on GluN2B-containing glutamate receptors. Molecular Pharmacology, 82(6), 1074-1081. https://doi.org/10.1124/mol.112.078998.Asztély F, Gustafsson B. Ionotropic glutamate receptors: Their possible role in the expression of hippocampal synaptic plasticity. Mol Neurobiol. 1996;12(1):1–11.Aizenman E, Frosch MP, Lipton SA. Responses mediated by excitatory amino acid receptors in solitary retinal ganglion cells from rat. J Physiol. 1988;396(1):75–91.Andreou LV. Isolation of plasmid DNA from bacteria. Methods Enzymol. 2013;529:135-42. doi: 10.1016/B978-0-12-418687-3.00010-0. PMID: 24011041.Albensi, B. C. (2007). The NMDA receptor/ion channel complex: a drug target for modulating synaptic plasticity and excitotoxicity. Current pharmaceutical design, 13(31), 3185-3194.Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., & Struhl, K. (Eds.). (2005). Current Protocols in Molecular Biology. John Wiley & Sons.Baez, S. D. L. C., García del Barco, D., Hardy-Sosa, A., Guillen Nieto, G., Bringas-Vega, M. L., Llibre-Guerra, J. J., & Valdes-Sosa, P. (2021). Scalable Bio marker combinations for early stroke diagnosis: A systematic review. Frontiers in neurology, 12, 638693.Beaudoin, G. M., Lee, S. H., Singh, D., Yuan, Y., Ng, Y. G., Reichardt, L. F., & Arikkath, J. (2012). Culturing pyramidal neurons from the early postnatal mouse hippocampus and cortex. Nature protocols, 7(9), 1741-1754.Beesley, S., Sullenberger, T., Pilli, J., Abbasi, S., Gunjan, A., & Kumar, S. S. (2019). Colocalization of distinct NMDA receptor subtypes at excitatory synapses in the entorhinal cortex. Journal of Neurophysiology, 121(1), 238-254. https://doi.org/10.1152/jn.00468.2018Bermúdez Urrego, Y. S. (2022). Evaluation of the effect of the Pb53a a synthetic peptide obtained from toxins of the spider Phoneutria boliviensis on the cell viability of the HEK293 line.Bermudez, S and Reyes-Guzman EA. (2020). Tesis de pregrado. Evaluation of the effect of the Pb53a, a synthetic peptide obtained from toxins of the spider Phoneutria boliviensis on the cell viability of the HEK-293 lineinstname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/The NMDA (N-methyl-D-aspartate) receptor plays a crucial role in synaptic function and neuronal plasticity, but its hyperactivation can lead to excitotoxicity, a phenomenon implicated in several neurodegenerative diseases. In this context, the use of peptides derived from animal toxins has emerged as a promising strategy to selectively modulate NMDA receptor activity and counteract excitotoxicity. These peptides, from diverse sources such as snakes and spiders, act as specific ligands of the NMDA receptor and can function as agonists, antagonists or allosteric modulators, interacting with different binding sites on the receptor.El receptor NMDA (N-metil-D-aspartato) desempeña un papel crucial en la función sináptica y la plasticidad neuronal, pero su hiperactivación puede llevar a la excitotoxicidad, un fenómeno implicado en diversas enfermedades neurodegenerativas. En este contexto, el uso de péptidos derivados de toxinas animales ha surgido como una estrategia prometedora para modular selectivamente la actividad del receptor NMDA y contrarrestar la excitotoxicidad.Bioquímico(a)PregradoPresencialInvestigaciónspaUniversidad Antonio NariñoBioquímicaFacultad de CienciasBogotá - CircunvalarBioquimica57438.23 D946eBiochemistryEvaluación de la expresión de subunidades recombinantes del receptor NMDA en la línea celular HEK-293.Trabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85GeneralORIGINAL2023_StephanyValentinaDuqueGuevara.pdf2023_StephanyValentinaDuqueGuevara.pdfapplication/pdf2781386https://repositorio.uan.edu.co/bitstreams/9c2701c0-b97b-4994-8cd5-8fd8dd202276/downloadec3c765f1906f40ee6f8bc13cf36ca97MD512023_StephanyValentinaDuqueGuevara_Formato Evaluación.pdf2023_StephanyValentinaDuqueGuevara_Formato Evaluación.pdfapplication/pdf133171https://repositorio.uan.edu.co/bitstreams/5604b8db-8442-4692-b9aa-574ea9a95000/download0708d6f4a400e43106585eed4fda0dedMD522023_StephanyValentinaDuqueGuevara_Acta.pdf2023_StephanyValentinaDuqueGuevara_Acta.pdfapplication/pdf1570047https://repositorio.uan.edu.co/bitstreams/df708e23-0049-4d83-a872-314fbb579d34/download680f5bb1a6889e73dffc6c64c5f90281MD532023_StephanyValentinaDuqueGuevara_Autorización.pdf2023_StephanyValentinaDuqueGuevara_Autorización.pdfapplication/pdf1687622https://repositorio.uan.edu.co/bitstreams/41b4c83c-3c18-4159-b5ee-7c458d6ae2b9/downloadc04a1ff266db9455b78703d2390f6effMD55CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.uan.edu.co/bitstreams/5533042d-9d3f-450e-b02d-22b9279f095b/download9868ccc48a14c8d591352b6eaf7f6239MD56123456789/8345oai:repositorio.uan.edu.co:123456789/83452024-10-09 22:37:16.637https://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertoopen.accesshttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co |