Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.

Cervical cancer has a high mortality rate among women, being one of the malignant neoplasms with the highest incidence. Current therapeutic options are highly invasive; For this reason, treatment alternatives with fewer side effects and invasiveness are necessary. Potential new therapeutic options i...

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Autores:: Torres Viñuela, Valeria

Tipo de recurso:: Trabajo de grado de pregrado

Fecha de publicación:: 2021

Institución:: Universidad Antonio Nariño

Repositorio:: Repositorio UAN

Idioma:: spa

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dc.title.es_ES.fl_str_mv	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
title	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
spellingShingle	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp. Polaromonas sp Péptidos antimicrobianos Péptidos anticancerígenos PHP-1 Cáncer de cuello uterino Citotoxicidad Apoptosis 540 Polaromonas sp Antimicrobial peptide Anticancer peptide PHP-1 Cervical cancer Cytotoxicity Apoptosis
title_short	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
title_full	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
title_fullStr	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
title_full_unstemmed	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
title_sort	Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.
dc.creator.fl_str_mv	Torres Viñuela, Valeria
dc.contributor.advisor.spa.fl_str_mv	Losada Barragán, Mónica
dc.contributor.author.spa.fl_str_mv	Torres Viñuela, Valeria
dc.subject.es_ES.fl_str_mv	Polaromonas sp Péptidos antimicrobianos Péptidos anticancerígenos PHP-1 Cáncer de cuello uterino Citotoxicidad Apoptosis
topic	Polaromonas sp Péptidos antimicrobianos Péptidos anticancerígenos PHP-1 Cáncer de cuello uterino Citotoxicidad Apoptosis 540 Polaromonas sp Antimicrobial peptide Anticancer peptide PHP-1 Cervical cancer Cytotoxicity Apoptosis
dc.subject.ddc.es_ES.fl_str_mv	540
dc.subject.keyword.es_ES.fl_str_mv	Polaromonas sp Antimicrobial peptide Anticancer peptide PHP-1 Cervical cancer Cytotoxicity Apoptosis
description	Cervical cancer has a high mortality rate among women, being one of the malignant neoplasms with the highest incidence. Current therapeutic options are highly invasive; For this reason, treatment alternatives with fewer side effects and invasiveness are necessary. Potential new therapeutic options include anticancer peptides and proteins derived from antimicrobial peptides. The purpose of this study was to evaluate the cytotoxic effect and the cell death mechanism of the hypothetical protein PHP-1 of the psychrophilic microorganism Polaromonas sp. in the cervical cancer cell line. Through bioinformatic analysis, it was shown that PHP-1 exhibits structural characteristics typical of anti-cancer peptides and proteins. The cytotoxicity of PHP-1 was evaluated by an MTT test that showed a dose and time-dependent effect. The effects generated were additionally analyzed by means of an annexin V / PI labeling, indicating that the hypothetical protein induces apoptosis, which correlates with the observed morphological changes. The results of this work represent the first functional study of the hypothetical protein PHP-1 and suggest that PHP-1 is cytotoxic for the HeLa cell line by inducing cell apoptosis, highlighting its potential as an anticancer agent.
publishDate	2021
dc.date.issued.spa.fl_str_mv	2021-11-24
dc.date.accessioned.none.fl_str_mv	2022-04-30T01:27:44Z
dc.date.available.none.fl_str_mv	2022-04-30T01:27:44Z
dc.type.spa.fl_str_mv	Trabajo de grado (Pregrado y/o Especialización)
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dc.identifier.uri.none.fl_str_mv	http://repositorio.uan.edu.co/handle/123456789/6378
dc.identifier.bibliographicCitation.spa.fl_str_mv	Baindara, P., Korpole, S., & Grover, V. (2018). Bacteriocins: Perspective for the development of novel anticancer drugs. Applied Microbiology and Biotechnology, 102(24), 10393–10408. https://doi.org/10.1007/s00253-018-9420-8 Bravo, L. E. (2016). Estimating the incidence and mortality of cancer in Colombia: What are the best data for public policies? Colombia Medica, 71–73. https://doi.org/10.25100/cm.v47i2.2381 Brogden, K. A. (2005). Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? Nature Reviews Microbiology, 3(3), 238–250. https://doi.org/10.1038/nrmicro1098 Castro-Jiménez, M. Á., Vera-Cala, L. M., & Posso-Valencia, H. J. (2006). Epidemiología del cáncer de cuello uterino: Estado del arte. Revista Colombiana de Obstetricia y Ginecología, 57(3), 182–189. https://doi.org/10.18597/rcog.489 Chao, A., Yen, T.-C., & Lai, C.-H. (2014). Diagnosis and staging of cervical cancer. Advances in Cervical Cancer Management, 56–67. https://doi.org/10.2217/fmeb2013.13.71 Chen, Y., Jiang, S., Jin, Y., Yin, Y., Yu, G., Lan, X., Cui, M., Liang, Y., Wong, B. H. C., Guo, L., & Sun, H. (2012). Purification and characterization of an antitumor protein with deoxyribonuclease activity from edible mushroom Agrocybe aegerita. Molecular Nutrition & Food Research, 56(11), 1729–1738. https://doi.org/10.1002/mnfr.201200316 Cibula, Pötter, Planchamp, Avall-Lundqvist, Fischerova, Haie-Meder, Köhler, Landoni, Lax, Lindegaard, Mahantshetty, Mathevet, McCluggage, McCormack, Naik, Nout, Pignata, Ponce, Querleu, … Raspollini. (2018). The european society of gynaecological oncology/european society for radiotherapy and oncology/european society of pathology guidelines for the management of patients with cervical cancer. Virchows Archiv, 472(6), 919–936. https://doi.org/10.1007/s00428-018-2362-9 Darcy, J. L., Lynch, R. C., King, A. J., Robeson, M. S., & Schmidt, S. K. (2011). Global distribution of polaromonas phylotypes - Evidence for a highly successful dispersal capacity. PLoS ONE, 6(8), e23742. https://doi.org/10.1371/journal.pone.0023742 de la Fuente-Núñez, C., Silva, O. N., Lu, T. K., & Franco, O. L. (2017). Antimicrobial peptides: Role in human disease and potential as immunotherapies. Pharmacology & Therapeutics, 178, 132–140. https://doi.org/10.1016/j.pharmthera.2017.04.002 Di Tommaso, P., Moretti, S., Xenarios, I., Orobitg, M., Montanyola, A., Chang, J.-M., Taly, J.-F., & Notredame, C. (2011). T-Coffee: A web server for the multiple sequence alignment of protein and RNA sequences using structural information and homology extension. Nucleic Acids Research, 39(suppl), W13–W17. https://doi.org/10.1093/nar/gkr245
dc.identifier.instname.spa.fl_str_mv	instname:Universidad Antonio Nariño
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identifier_str_mv	Baindara, P., Korpole, S., & Grover, V. (2018). Bacteriocins: Perspective for the development of novel anticancer drugs. Applied Microbiology and Biotechnology, 102(24), 10393–10408. https://doi.org/10.1007/s00253-018-9420-8 Bravo, L. E. (2016). Estimating the incidence and mortality of cancer in Colombia: What are the best data for public policies? Colombia Medica, 71–73. https://doi.org/10.25100/cm.v47i2.2381 Brogden, K. A. (2005). Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? Nature Reviews Microbiology, 3(3), 238–250. https://doi.org/10.1038/nrmicro1098 Castro-Jiménez, M. Á., Vera-Cala, L. M., & Posso-Valencia, H. J. (2006). Epidemiología del cáncer de cuello uterino: Estado del arte. Revista Colombiana de Obstetricia y Ginecología, 57(3), 182–189. https://doi.org/10.18597/rcog.489 Chao, A., Yen, T.-C., & Lai, C.-H. (2014). Diagnosis and staging of cervical cancer. Advances in Cervical Cancer Management, 56–67. https://doi.org/10.2217/fmeb2013.13.71 Chen, Y., Jiang, S., Jin, Y., Yin, Y., Yu, G., Lan, X., Cui, M., Liang, Y., Wong, B. H. C., Guo, L., & Sun, H. (2012). Purification and characterization of an antitumor protein with deoxyribonuclease activity from edible mushroom Agrocybe aegerita. Molecular Nutrition & Food Research, 56(11), 1729–1738. https://doi.org/10.1002/mnfr.201200316 Cibula, Pötter, Planchamp, Avall-Lundqvist, Fischerova, Haie-Meder, Köhler, Landoni, Lax, Lindegaard, Mahantshetty, Mathevet, McCluggage, McCormack, Naik, Nout, Pignata, Ponce, Querleu, … Raspollini. (2018). The european society of gynaecological oncology/european society for radiotherapy and oncology/european society of pathology guidelines for the management of patients with cervical cancer. Virchows Archiv, 472(6), 919–936. https://doi.org/10.1007/s00428-018-2362-9 Darcy, J. L., Lynch, R. C., King, A. J., Robeson, M. S., & Schmidt, S. K. (2011). Global distribution of polaromonas phylotypes - Evidence for a highly successful dispersal capacity. PLoS ONE, 6(8), e23742. https://doi.org/10.1371/journal.pone.0023742 de la Fuente-Núñez, C., Silva, O. N., Lu, T. K., & Franco, O. L. (2017). Antimicrobial peptides: Role in human disease and potential as immunotherapies. Pharmacology & Therapeutics, 178, 132–140. https://doi.org/10.1016/j.pharmthera.2017.04.002 Di Tommaso, P., Moretti, S., Xenarios, I., Orobitg, M., Montanyola, A., Chang, J.-M., Taly, J.-F., & Notredame, C. (2011). T-Coffee: A web server for the multiple sequence alignment of protein and RNA sequences using structural information and homology extension. Nucleic Acids Research, 39(suppl), W13–W17. https://doi.org/10.1093/nar/gkr245 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/
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eu_rights_str_mv	openAccess
dc.publisher.spa.fl_str_mv	Universidad Antonio Nariño
dc.publisher.program.spa.fl_str_mv	Bioquímica
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.campus.spa.fl_str_mv	Bogotá - Circunvalar
institution	Universidad Antonio Nariño
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spelling	Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Losada Barragán, MónicaTorres Viñuela, Valeria118217137012022-04-30T01:27:44Z2022-04-30T01:27:44Z2021-11-24http://repositorio.uan.edu.co/handle/123456789/6378Baindara, P., Korpole, S., & Grover, V. (2018). Bacteriocins: Perspective for the development of novel anticancer drugs. Applied Microbiology and Biotechnology, 102(24), 10393–10408. https://doi.org/10.1007/s00253-018-9420-8Bravo, L. E. (2016). Estimating the incidence and mortality of cancer in Colombia: What are the best data for public policies? Colombia Medica, 71–73. https://doi.org/10.25100/cm.v47i2.2381Brogden, K. A. (2005). Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? Nature Reviews Microbiology, 3(3), 238–250. https://doi.org/10.1038/nrmicro1098Castro-Jiménez, M. Á., Vera-Cala, L. M., & Posso-Valencia, H. J. (2006). Epidemiología del cáncer de cuello uterino: Estado del arte. Revista Colombiana de Obstetricia y Ginecología, 57(3), 182–189. https://doi.org/10.18597/rcog.489Chao, A., Yen, T.-C., & Lai, C.-H. (2014). Diagnosis and staging of cervical cancer. Advances in Cervical Cancer Management, 56–67. https://doi.org/10.2217/fmeb2013.13.71Chen, Y., Jiang, S., Jin, Y., Yin, Y., Yu, G., Lan, X., Cui, M., Liang, Y., Wong, B. H. C., Guo, L., & Sun, H. (2012). Purification and characterization of an antitumor protein with deoxyribonuclease activity from edible mushroom Agrocybe aegerita. Molecular Nutrition & Food Research, 56(11), 1729–1738. https://doi.org/10.1002/mnfr.201200316Cibula, Pötter, Planchamp, Avall-Lundqvist, Fischerova, Haie-Meder, Köhler, Landoni, Lax, Lindegaard, Mahantshetty, Mathevet, McCluggage, McCormack, Naik, Nout, Pignata, Ponce, Querleu, … Raspollini. (2018). The european society of gynaecological oncology/european society for radiotherapy and oncology/european society of pathology guidelines for the management of patients with cervical cancer. Virchows Archiv, 472(6), 919–936. https://doi.org/10.1007/s00428-018-2362-9Darcy, J. L., Lynch, R. C., King, A. J., Robeson, M. S., & Schmidt, S. K. (2011). Global distribution of polaromonas phylotypes - Evidence for a highly successful dispersal capacity. PLoS ONE, 6(8), e23742. https://doi.org/10.1371/journal.pone.0023742de la Fuente-Núñez, C., Silva, O. N., Lu, T. K., & Franco, O. L. (2017). Antimicrobial peptides: Role in human disease and potential as immunotherapies. Pharmacology & Therapeutics, 178, 132–140. https://doi.org/10.1016/j.pharmthera.2017.04.002Di Tommaso, P., Moretti, S., Xenarios, I., Orobitg, M., Montanyola, A., Chang, J.-M., Taly, J.-F., & Notredame, C. (2011). T-Coffee: A web server for the multiple sequence alignment of protein and RNA sequences using structural information and homology extension. Nucleic Acids Research, 39(suppl), W13–W17. https://doi.org/10.1093/nar/gkr245instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/Cervical cancer has a high mortality rate among women, being one of the malignant neoplasms with the highest incidence. Current therapeutic options are highly invasive; For this reason, treatment alternatives with fewer side effects and invasiveness are necessary. Potential new therapeutic options include anticancer peptides and proteins derived from antimicrobial peptides. The purpose of this study was to evaluate the cytotoxic effect and the cell death mechanism of the hypothetical protein PHP-1 of the psychrophilic microorganism Polaromonas sp. in the cervical cancer cell line. Through bioinformatic analysis, it was shown that PHP-1 exhibits structural characteristics typical of anti-cancer peptides and proteins. The cytotoxicity of PHP-1 was evaluated by an MTT test that showed a dose and time-dependent effect. The effects generated were additionally analyzed by means of an annexin V / PI labeling, indicating that the hypothetical protein induces apoptosis, which correlates with the observed morphological changes. The results of this work represent the first functional study of the hypothetical protein PHP-1 and suggest that PHP-1 is cytotoxic for the HeLa cell line by inducing cell apoptosis, highlighting its potential as an anticancer agent.El cáncer del cuello uterino presenta un alto índice de mortalidad entre las mujeres, siendo una de las neoplasias malignas con mayor incidencia. Las opciones terapéuticas actuales son altamente invasivas; por esto son necesarias alternativas de tratamiento con menores efectos secundarios e invasividad. Entre las nuevas posibles opciones terapéuticas se encuentran los péptidos y proteínas anticancerígenas derivadas de péptidos antimicrobianos. El propósito de este estudio fue evaluar el efecto citotóxico y el mecanismo de muerte celular de la proteína hipotética PHP-1 del microorganismo psicrófilo Polaromonas sp. en la línea celular de cáncer cérvico-uterino. Por medio de análisis bioinformáticos, se demostró que la PHP-1 exhibe características estructurales propias de los péptidos y proteínas anticancerígenas. La citotoxicidad de la PHP 1 fue evaluada mediante un ensayo MTT que mostró un efecto dosis y tiempo dependiente. Los efectos generados fueron complementariamente analizados por medio de una marcación de anexina V/PI, indicando que la proteína hipotética induce apoptosis, lo cual correlaciona con los cambios morfológicos observados. Los resultados de este trabajo representan el primer estudio funcional de la proteína hipotética PHP-1 y sugieren que la PHP-1 es citotóxica para la línea celular HeLa mediante la inducción de apoptosis celular, destacando su potencial como agente anticancerígeno.Bioquímico(a)PregradoPresencialInvestigaciónspaUniversidad Antonio NariñoBioquímicaFacultad de CienciasBogotá - CircunvalarPolaromonas spPéptidos antimicrobianosPéptidos anticancerígenosPHP-1Cáncer de cuello uterinoCitotoxicidadApoptosis540Polaromonas spAntimicrobial peptideAnticancer peptidePHP-1Cervical cancerCytotoxicityApoptosisEvaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.Trabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85EspecializadaORIGINAL2021_ValeriaTorresViñuela_Acta.pdf2021_ValeriaTorresViñuela_Acta.pdfActa de sustentaciónapplication/pdf504650https://repositorio.uan.edu.co/bitstreams/e4e12c8e-574f-4585-8d98-f9004117facb/download33b292ce1ec29b9c1f93af1c6b979a18MD512021_ValeriaTorresViñuela_Autorización.pdf2021_ValeriaTorresViñuela_Autorización.pdfFormato autorización de autoresapplication/pdf821041https://repositorio.uan.edu.co/bitstreams/c4c9a793-eaee-4b7c-8885-93696fbbc5b9/download1f84e877d5be4df3a5f27e1c7be07a30MD522021_ValeriaTorresVIñuela.pdf2021_ValeriaTorresVIñuela.pdfTrabajo de gradoapplication/pdf647585https://repositorio.uan.edu.co/bitstreams/b568f621-b412-4950-ba77-519830d91509/download14948cd086928066a1446fc2cf00b628MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.uan.edu.co/bitstreams/75e07b33-8102-47f5-8de5-e36ef21bdcd1/download9868ccc48a14c8d591352b6eaf7f6239MD54123456789/6378oai:repositorio.uan.edu.co:123456789/63782024-10-09 22:56:49.757https://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertorestrictedhttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co

Evaluación de la actividad anticancerígena de una proteína hipotética recombinante (PHP- 1) de Polaromonas sp.

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