Recomendaciones para la evaluación de las propiedades biomecánicas de hidrogeles de fibrina agarosa nanoestructurado por medio de microscopia de fuerza atómica

Propia

Autores:: Ospina Toro, Lucila
Muñoz Tocua, Sandra Patricia

Tipo de recurso:: Trabajo de grado de pregrado

Fecha de publicación:: 2020

Institución:: Universidad Antonio Nariño

Repositorio:: Repositorio UAN

Idioma:: spa

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dc.title.es_ES.fl_str_mv	Recomendaciones para la evaluación de las propiedades biomecánicas de hidrogeles de fibrina agarosa nanoestructurado por medio de microscopia de fuerza atómica
title	Recomendaciones para la evaluación de las propiedades biomecánicas de hidrogeles de fibrina agarosa nanoestructurado por medio de microscopia de fuerza atómica
spellingShingle	Recomendaciones para la evaluación de las propiedades biomecánicas de hidrogeles de fibrina agarosa nanoestructurado por medio de microscopia de fuerza atómica Ingeniería de tejidos, Agarosa, fibrina, andamios del tejido, materiales biocompatibles, regeneración tisular dirigida, microscopía de fuerza atómica, hidrogeles. Tissue Engineering, agarose, fibrin, scaffolds, Guided Tissue Regeneration, atomic force microscopy,
title_short	Recomendaciones para la evaluación de las propiedades biomecánicas de hidrogeles de fibrina agarosa nanoestructurado por medio de microscopia de fuerza atómica
title_full	Recomendaciones para la evaluación de las propiedades biomecánicas de hidrogeles de fibrina agarosa nanoestructurado por medio de microscopia de fuerza atómica
title_fullStr	Recomendaciones para la evaluación de las propiedades biomecánicas de hidrogeles de fibrina agarosa nanoestructurado por medio de microscopia de fuerza atómica
title_full_unstemmed	Recomendaciones para la evaluación de las propiedades biomecánicas de hidrogeles de fibrina agarosa nanoestructurado por medio de microscopia de fuerza atómica
title_sort	Recomendaciones para la evaluación de las propiedades biomecánicas de hidrogeles de fibrina agarosa nanoestructurado por medio de microscopia de fuerza atómica
dc.creator.fl_str_mv	Ospina Toro, Lucila Muñoz Tocua, Sandra Patricia
dc.contributor.advisor.spa.fl_str_mv	Alfonso Rodríguez, Camilo Andrés Jaimes Monroy, Gustavo Jaramillo, Sebastian Gutierrez, David
dc.contributor.author.spa.fl_str_mv	Ospina Toro, Lucila Muñoz Tocua, Sandra Patricia
dc.subject.es_ES.fl_str_mv	Ingeniería de tejidos, Agarosa, fibrina, andamios del tejido, materiales biocompatibles, regeneración tisular dirigida, microscopía de fuerza atómica, hidrogeles.
topic	Ingeniería de tejidos, Agarosa, fibrina, andamios del tejido, materiales biocompatibles, regeneración tisular dirigida, microscopía de fuerza atómica, hidrogeles. Tissue Engineering, agarose, fibrin, scaffolds, Guided Tissue Regeneration, atomic force microscopy,
dc.subject.keyword.es_ES.fl_str_mv	Tissue Engineering, agarose, fibrin, scaffolds, Guided Tissue Regeneration, atomic force microscopy,
description	Propia
publishDate	2020
dc.date.issued.spa.fl_str_mv	2020-11-19
dc.date.accessioned.none.fl_str_mv	2021-03-03T22:23:53Z
dc.date.available.none.fl_str_mv	2021-03-03T22:23:53Z
dc.type.spa.fl_str_mv	Trabajo de grado (Pregrado y/o Especialización)
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dc.identifier.bibliographicCitation.spa.fl_str_mv	1. Hassanzadeh P, Atyabi F, Dinarvand R. Tissue engineering: Still facing a long way ahead. J Control Release. 2018;279:181-97. 2. O´Brien F. Biomaterials & scaffolds for tissue engineering. Materials Today. 2011;14(3):88-95. 3. Hollister SJ. Scaffold engineering: a bridge to where? Biofabrication. 2009;1(1):012001. 4. Beitzel K, McCarthy MB, Cote MP, Russell RP, Apostolakos J, Ramos DM, et al. Properties of Biologic Scaffolds and Their Response to Mesenchymal Stem Cells. Arthroscopy: The Journal of Arthroscopic & Related Surgery. 2014;30(3):289-98. 5. Zuidema JM, Rivet CJ, Gilbert RJ, Morrison FA. A protocol for rheological characterization of hydrogels for tissue engineering strategies. J Biomed Mater Res B Appl Biomater. 2014;102(5):1063-73. 6. Chato-Astrain J, Campos F, Roda O, Miralles E, Durand-Herrera D, Saez-Moreno JA, et al. In vivo Evaluation of Nanostructured Fibrin-Agarose Hydrogels With Mesenchymal Stem Cells for Peripheral Nerve Repair. Front Cell Neurosci. 2018;12:501. 7. Giuseppe MD, Law N, Webb B, R AM, Liew LJ, Sercombe TB, et al. Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting. J Mech Behav Biomed Mater. 2018;79:150-7. 8. Carriel V, Vizcaino-Lopez G, Chato-Astrain J, Durand-Herrera D, Alaminos M, Campos A, et al. Scleral surgical repair through the use of nanostructured fibrin/agarose-based films in rabbits. Exp Eye Res. 2019;186:107717.1. Hassanzadeh P, Atyabi F, Dinarvand R. Tissue engineering: Still facing a long way ahead. J Control Release. 2018;279:181-97. 2. O´Brien F. Biomaterials & scaffolds for tissue engineering. Materials Today. 2011;14(3):88-95. 3. Hollister SJ. Scaffold engineering: a bridge to where? Biofabrication. 2009;1(1):012001. 4. Beitzel K, McCarthy MB, Cote MP, Russell RP, Apostolakos J, Ramos DM, et al. Properties of Biologic Scaffolds and Their Response to Mesenchymal Stem Cells. Arthroscopy: The Journal of Arthroscopic & Related Surgery. 2014;30(3):289-98. 5. Zuidema JM, Rivet CJ, Gilbert RJ, Morrison FA. A protocol for rheological characterization of hydrogels for tissue engineering strategies. J Biomed Mater Res B Appl Biomater. 2014;102(5):1063-73. 6. Chato-Astrain J, Campos F, Roda O, Miralles E, Durand-Herrera D, Saez-Moreno JA, et al. In vivo Evaluation of Nanostructured Fibrin-Agarose Hydrogels With Mesenchymal Stem Cells for Peripheral Nerve Repair. Front Cell Neurosci. 2018;12:501. 7. Giuseppe MD, Law N, Webb B, R AM, Liew LJ, Sercombe TB, et al. Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting. J Mech Behav Biomed Mater. 2018;79:150-7. 8. Carriel V, Vizcaino-Lopez G, Chato-Astrain J, Durand-Herrera D, Alaminos M, Campos A, et al. Scleral surgical repair through the use of nanostructured fibrin/agarose-based films in rabbits. Exp Eye Res. 2019;186:107717.
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identifier_str_mv	1. Hassanzadeh P, Atyabi F, Dinarvand R. Tissue engineering: Still facing a long way ahead. J Control Release. 2018;279:181-97. 2. O´Brien F. Biomaterials & scaffolds for tissue engineering. Materials Today. 2011;14(3):88-95. 3. Hollister SJ. Scaffold engineering: a bridge to where? Biofabrication. 2009;1(1):012001. 4. Beitzel K, McCarthy MB, Cote MP, Russell RP, Apostolakos J, Ramos DM, et al. Properties of Biologic Scaffolds and Their Response to Mesenchymal Stem Cells. Arthroscopy: The Journal of Arthroscopic & Related Surgery. 2014;30(3):289-98. 5. Zuidema JM, Rivet CJ, Gilbert RJ, Morrison FA. A protocol for rheological characterization of hydrogels for tissue engineering strategies. J Biomed Mater Res B Appl Biomater. 2014;102(5):1063-73. 6. Chato-Astrain J, Campos F, Roda O, Miralles E, Durand-Herrera D, Saez-Moreno JA, et al. In vivo Evaluation of Nanostructured Fibrin-Agarose Hydrogels With Mesenchymal Stem Cells for Peripheral Nerve Repair. Front Cell Neurosci. 2018;12:501. 7. Giuseppe MD, Law N, Webb B, R AM, Liew LJ, Sercombe TB, et al. Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting. J Mech Behav Biomed Mater. 2018;79:150-7. 8. Carriel V, Vizcaino-Lopez G, Chato-Astrain J, Durand-Herrera D, Alaminos M, Campos A, et al. Scleral surgical repair through the use of nanostructured fibrin/agarose-based films in rabbits. Exp Eye Res. 2019;186:107717.1. Hassanzadeh P, Atyabi F, Dinarvand R. Tissue engineering: Still facing a long way ahead. J Control Release. 2018;279:181-97. 2. O´Brien F. Biomaterials & scaffolds for tissue engineering. Materials Today. 2011;14(3):88-95. 3. Hollister SJ. Scaffold engineering: a bridge to where? Biofabrication. 2009;1(1):012001. 4. Beitzel K, McCarthy MB, Cote MP, Russell RP, Apostolakos J, Ramos DM, et al. Properties of Biologic Scaffolds and Their Response to Mesenchymal Stem Cells. Arthroscopy: The Journal of Arthroscopic & Related Surgery. 2014;30(3):289-98. 5. Zuidema JM, Rivet CJ, Gilbert RJ, Morrison FA. A protocol for rheological characterization of hydrogels for tissue engineering strategies. J Biomed Mater Res B Appl Biomater. 2014;102(5):1063-73. 6. Chato-Astrain J, Campos F, Roda O, Miralles E, Durand-Herrera D, Saez-Moreno JA, et al. In vivo Evaluation of Nanostructured Fibrin-Agarose Hydrogels With Mesenchymal Stem Cells for Peripheral Nerve Repair. Front Cell Neurosci. 2018;12:501. 7. Giuseppe MD, Law N, Webb B, R AM, Liew LJ, Sercombe TB, et al. Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting. J Mech Behav Biomed Mater. 2018;79:150-7. 8. Carriel V, Vizcaino-Lopez G, Chato-Astrain J, Durand-Herrera D, Alaminos M, Campos A, et al. Scleral surgical repair through the use of nanostructured fibrin/agarose-based films in rabbits. Exp Eye Res. 2019;186:107717. instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/
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dc.publisher.spa.fl_str_mv	Universidad Antonio Nariño
dc.publisher.program.spa.fl_str_mv	Especialización en Periodoncia
dc.publisher.faculty.spa.fl_str_mv	Facultad de Odontología
dc.publisher.campus.spa.fl_str_mv	Bogotá - Circunvalar
institution	Universidad Antonio Nariño
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spelling	Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Alfonso Rodríguez, Camilo AndrésJaimes Monroy, GustavoJaramillo, SebastianGutierrez, DavidOspina Toro, LucilaMuñoz Tocua, Sandra Patricia0000-0001-7243-9272http://scienti.colciencias.gov.co:8081/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001619222805412392021-03-03T22:23:53Z2021-03-03T22:23:53Z2020-11-19http://repositorio.uan.edu.co/handle/123456789/26171. Hassanzadeh P, Atyabi F, Dinarvand R. Tissue engineering: Still facing a long way ahead. J Control Release. 2018;279:181-97. 2. O´Brien F. Biomaterials & scaffolds for tissue engineering. Materials Today. 2011;14(3):88-95. 3. Hollister SJ. Scaffold engineering: a bridge to where? Biofabrication. 2009;1(1):012001. 4. Beitzel K, McCarthy MB, Cote MP, Russell RP, Apostolakos J, Ramos DM, et al. Properties of Biologic Scaffolds and Their Response to Mesenchymal Stem Cells. Arthroscopy: The Journal of Arthroscopic & Related Surgery. 2014;30(3):289-98. 5. Zuidema JM, Rivet CJ, Gilbert RJ, Morrison FA. A protocol for rheological characterization of hydrogels for tissue engineering strategies. J Biomed Mater Res B Appl Biomater. 2014;102(5):1063-73. 6. Chato-Astrain J, Campos F, Roda O, Miralles E, Durand-Herrera D, Saez-Moreno JA, et al. In vivo Evaluation of Nanostructured Fibrin-Agarose Hydrogels With Mesenchymal Stem Cells for Peripheral Nerve Repair. Front Cell Neurosci. 2018;12:501. 7. Giuseppe MD, Law N, Webb B, R AM, Liew LJ, Sercombe TB, et al. Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting. J Mech Behav Biomed Mater. 2018;79:150-7. 8. Carriel V, Vizcaino-Lopez G, Chato-Astrain J, Durand-Herrera D, Alaminos M, Campos A, et al. Scleral surgical repair through the use of nanostructured fibrin/agarose-based films in rabbits. Exp Eye Res. 2019;186:107717.1. Hassanzadeh P, Atyabi F, Dinarvand R. Tissue engineering: Still facing a long way ahead. J Control Release. 2018;279:181-97. 2. O´Brien F. Biomaterials & scaffolds for tissue engineering. Materials Today. 2011;14(3):88-95. 3. Hollister SJ. Scaffold engineering: a bridge to where? Biofabrication. 2009;1(1):012001. 4. Beitzel K, McCarthy MB, Cote MP, Russell RP, Apostolakos J, Ramos DM, et al. Properties of Biologic Scaffolds and Their Response to Mesenchymal Stem Cells. Arthroscopy: The Journal of Arthroscopic & Related Surgery. 2014;30(3):289-98. 5. Zuidema JM, Rivet CJ, Gilbert RJ, Morrison FA. A protocol for rheological characterization of hydrogels for tissue engineering strategies. J Biomed Mater Res B Appl Biomater. 2014;102(5):1063-73. 6. Chato-Astrain J, Campos F, Roda O, Miralles E, Durand-Herrera D, Saez-Moreno JA, et al. In vivo Evaluation of Nanostructured Fibrin-Agarose Hydrogels With Mesenchymal Stem Cells for Peripheral Nerve Repair. Front Cell Neurosci. 2018;12:501. 7. Giuseppe MD, Law N, Webb B, R AM, Liew LJ, Sercombe TB, et al. Mechanical behaviour of alginate-gelatin hydrogels for 3D bioprinting. J Mech Behav Biomed Mater. 2018;79:150-7. 8. Carriel V, Vizcaino-Lopez G, Chato-Astrain J, Durand-Herrera D, Alaminos M, Campos A, et al. Scleral surgical repair through the use of nanostructured fibrin/agarose-based films in rabbits. Exp Eye Res. 2019;186:107717.instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/PropiaIntroduction: The nanostructured agarose-fibrin is a material recently used in bioengineering applications, but the physical, mechanical and surface topographic characteristics at the nanoscale are still unknown. Objective. Is to review the theory and contrast with some previous laboratory results in relation to tests on hydrogel fibrin agarose scaffolds for the values of perforation force, adhesion force, as physical properties and apparent modulus of elasticity as mechanical property by evaluation with microscopy of atomic force. Methods: Samples of agarose-fibrin hydrogels were obtained, and a preliminary analysis was carried out in atomic force microscopy (AFM). Articles from the Pubmeds, google scholar, and sciencedirect databases were reviewed with search algorithms to extract information about the analysis parameters in AFM in a fibrin-agarose hydrogel sample. Results. Preliminary tests showed that the uncoated pyramid tip showed better penetration into the material surface without creating tears or adhesion to the material and an adhesive was not required to fix the samples due to the characteristic of the material. The theoretical review showed that for hydrogel samples, the ideal is to do AFM analysis in liquid medium with a buffer, which can also be used as a means of transport. In the same way, it is recommended to use low loads and a constant between k = 0.01-0.9 N / m. The ideal contact time is 100ms and a maximum force of 100pN for each indentation. Conclusions: Atomic f microscopy can be used for the analysis on a nanometric scale of fibrin agarose gels, but it requires establishing standardized parameters to avoid inconveniences with the samples.Introducción: La agarosa nanoestructurada con fibrina es un material usado recientemente en aplicaciones de bioingeniería, pero aún no conocen las propiedades físicas, mecánicas y las características topográficas de superficie en nanoescala. Objetivo. revisar la teoría y contrastar con algunos resultados previos de laboratorio en relación con ensayos sobre andamios de hidrogeles de fibrina agarosa para los valores de fuerza de perforación, fuerza de adhesión, como propiedades físicas y módulo de elasticidad aparente como propiedad mecánica por valoración con microscopía de fuerza atómica. Métodos: Se obtuvieron muestras de hidrogeles fibrina agarosa y se hizo un análisis preliminar en microscopia de fuerza atómica (AFM). Se revisaron artículos de las bases de datos Pubmed, google scholar y sciencedirect, con unos algoritmos de búsqueda para extraer la información acerca de los parámetros de análisis en AFM en muestra de hidrogeles de fibrina-agarosa. Resultados. Las pruebas preliminares mostraron que la punta piramidal sin recubrimiento mostró una mejor penetración en la superficie del material sin generar desgarros o adhesión al material y no se requirió un adhesivo para fijar las muestras debido a las características del material. La revisión teórica mostró que para muestras de hidrogeles lo ideal es hacer análisis de AFM en medio líquido con un buffer, que también puede ser usado como medio de transporte. De igual forma se recomienda usar cargas bajas y una constante entre k=0,01-0,9 N/m. El tiempo de contacto ideal es de 100ms y una fuerza máxima de 100 pN por cada indentación. Conclusiones: La Microscopía de Fuerza Atómica se puede usar para el análisis en escala nanométrica de los geles de fibrina agarosa, pero requiere establecer unos parámetros estandarizados para evitar inconvenientes con las muestras.Especialista en PeriodonciaEspecializaciónPresencialspaUniversidad Antonio NariñoEspecialización en PeriodonciaFacultad de OdontologíaBogotá - CircunvalarIngeniería de tejidos, Agarosa, fibrina, andamios del tejido, materiales biocompatibles, regeneración tisular dirigida, microscopía de fuerza atómica, hidrogeles.Tissue Engineering, agarose, fibrin, scaffolds, Guided Tissue Regeneration, atomic force microscopy,Recomendaciones para la evaluación de las propiedades biomecánicas de hidrogeles de fibrina agarosa nanoestructurado por medio de microscopia de fuerza atómicaTrabajo de grado (Pregrado y/o 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