Producción de cristales parasporales de Bacillus thuringiensis con potencial actividad anticancerígena
The species of the genus Bacillus are Gram positive bacteria and have a great capacity to form endospores. B. thuringiensis is one of the most studied species for its potential to produce cry proteins with cytotoxic activity against insects. Another group of proteins, parasporins that are formed as...
- Autores:
-
López Quiroz, Angie Vivian
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2021
- Institución:
- Universidad Antonio Nariño
- Repositorio:
- Repositorio UAN
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uan.edu.co:123456789/6370
- Acceso en línea:
- http://repositorio.uan.edu.co/handle/123456789/6370
- Palabra clave:
- Parasporinas
Bacillus thuringiensis
Virus del papiloma humano (VPH)
Cáncer
PCR
540
Parasporin
Bacillus thuringiensis
human papillomavirus
Cancer
PCR
- Rights
- openAccess
- License
- Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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Producción de cristales parasporales de Bacillus thuringiensis con potencial actividad anticancerígena |
| title |
Producción de cristales parasporales de Bacillus thuringiensis con potencial actividad anticancerígena |
| spellingShingle |
Producción de cristales parasporales de Bacillus thuringiensis con potencial actividad anticancerígena Parasporinas Bacillus thuringiensis Virus del papiloma humano (VPH) Cáncer PCR 540 Parasporin Bacillus thuringiensis human papillomavirus Cancer PCR |
| title_short |
Producción de cristales parasporales de Bacillus thuringiensis con potencial actividad anticancerígena |
| title_full |
Producción de cristales parasporales de Bacillus thuringiensis con potencial actividad anticancerígena |
| title_fullStr |
Producción de cristales parasporales de Bacillus thuringiensis con potencial actividad anticancerígena |
| title_full_unstemmed |
Producción de cristales parasporales de Bacillus thuringiensis con potencial actividad anticancerígena |
| title_sort |
Producción de cristales parasporales de Bacillus thuringiensis con potencial actividad anticancerígena |
| dc.creator.fl_str_mv |
López Quiroz, Angie Vivian |
| dc.contributor.advisor.spa.fl_str_mv |
Cuervo Soto, Laura Inés |
| dc.contributor.author.spa.fl_str_mv |
López Quiroz, Angie Vivian |
| dc.subject.es_ES.fl_str_mv |
Parasporinas Bacillus thuringiensis Virus del papiloma humano (VPH) Cáncer PCR |
| topic |
Parasporinas Bacillus thuringiensis Virus del papiloma humano (VPH) Cáncer PCR 540 Parasporin Bacillus thuringiensis human papillomavirus Cancer PCR |
| dc.subject.ddc.es_ES.fl_str_mv |
540 |
| dc.subject.keyword.es_ES.fl_str_mv |
Parasporin Bacillus thuringiensis human papillomavirus Cancer PCR |
| description |
The species of the genus Bacillus are Gram positive bacteria and have a great capacity to form endospores. B. thuringiensis is one of the most studied species for its potential to produce cry proteins with cytotoxic activity against insects. Another group of proteins, parasporins that are formed as parasporal inclusion, show activity against cancer cells, but not against normal cells. 19 parasporins have been identified grouped into 6 families (PS1, PS2, PS3, PS4, PS5 and PS6), with sizes from 30 to 88 kDa, whose activity has been demonstrated on different lines of cancer in humans. Cervical cancer is a type of silent cancer related to the human papillomavirus (HPV), for which existing treatments are not entirely effective. Therefore, the purpose of this study was to obtain parasporal crystals from cell extracts of B. thuringiensis strains, which showed protein values of 8 to 115 μg / mL, a concentration that can be considered optimal for cytotoxicity tests. In addition, bands of proteins related to parasporins were identified by SDS-PAGE electrophoresis, with approximate sizes of 30, 33, 37, 70 and 88 kDa that can correspond to parasporins of all families. These results indicate that these proteins can be tested especially in cervical cancer cells as an alternative for an early and effective treatment. |
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2021 |
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2021-11-26 |
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2022-04-29T20:52:32Z |
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2022-04-29T20:52:32Z |
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Trabajo de grado (Pregrado y/o Especialización) |
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http://repositorio.uan.edu.co/handle/123456789/6370 |
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Adang. M; Crickmore, N; & Jurat Fuentes, JL. (2014). Diversity de Bacillus thuringiensis Crystal Toxins and Mechanism of Action. Chapter two In: Insect midgut and insecticidal proteins, volumen 47. p. 39-87. Akiba, T; Abe, Y; Kitada, S; Kusaka, Y; Ito, A; Ichimatsu, T; Katayama, H; Akao, T; Higuchi, K; Mizuki, E; Ohba, M; Kanai, R; & Harata, K. (2009). Crystal Structure of the Parasporin-2 Bacillus thuringiensis Toxin That Recognizes Cancer Cells. Journal of Molecular Biology, 386,121-133. Akiba T, Okumura S. (2017). Parasporins 1 and 2: Their structure and activity. J Invertebr Pathol. 142:44-49. doi: 10.1016/j.jip.2016.10.005. Avignone-Rossa, C. & Mignone, C. (1995) Bacillus thuringiensis Growth and Toxicity. Molecular biotechnology. 4(1), 55-71. doi: 10.1007/BF02907471 Bradford Mm. A Rapid And Sensitive Method For The Quantitation Of Microgram Quantities Of Protein Utilizing The Principle Of Protein-Dye Binding. Anal Biochem. 1976, Vol. 7, 72. Bravo A., Gill S.S., Soberón M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon. 2007;49:423–435. doi: 10.1016/j.toxicon.2006.11.022. Centers for Disease Control and Prevention (CDCP) (2021). Tratamientos contra el cáncer. Retrieved 9 November 2021, from https://www.cdc.gov/spanish/cancer/survivors/patients/treatments.htm. Chatterjee A. The next generation of HPV vaccines: Nonavalent vaccine V503 on the horizon. Expert Review of Vaccines 2014; 13(11):1279-90. Chaturvedi AK, Engels EA, Pfeiffer RM, et al. Human papillomavirus and rising oropharyngeal cancer incidence in the United States. Journal of Clinical Oncology 2011; 29(32):4294–4301. Crosbie EJ, Einstein MH, Franceschi S, Kitchener HC. Human papillomavirus and cervical cancer. Lancet. 2013; 382: 889-899. |
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Adang. M; Crickmore, N; & Jurat Fuentes, JL. (2014). Diversity de Bacillus thuringiensis Crystal Toxins and Mechanism of Action. Chapter two In: Insect midgut and insecticidal proteins, volumen 47. p. 39-87. Akiba, T; Abe, Y; Kitada, S; Kusaka, Y; Ito, A; Ichimatsu, T; Katayama, H; Akao, T; Higuchi, K; Mizuki, E; Ohba, M; Kanai, R; & Harata, K. (2009). Crystal Structure of the Parasporin-2 Bacillus thuringiensis Toxin That Recognizes Cancer Cells. Journal of Molecular Biology, 386,121-133. Akiba T, Okumura S. (2017). Parasporins 1 and 2: Their structure and activity. J Invertebr Pathol. 142:44-49. doi: 10.1016/j.jip.2016.10.005. Avignone-Rossa, C. & Mignone, C. (1995) Bacillus thuringiensis Growth and Toxicity. Molecular biotechnology. 4(1), 55-71. doi: 10.1007/BF02907471 Bradford Mm. A Rapid And Sensitive Method For The Quantitation Of Microgram Quantities Of Protein Utilizing The Principle Of Protein-Dye Binding. Anal Biochem. 1976, Vol. 7, 72. Bravo A., Gill S.S., Soberón M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon. 2007;49:423–435. doi: 10.1016/j.toxicon.2006.11.022. Centers for Disease Control and Prevention (CDCP) (2021). Tratamientos contra el cáncer. Retrieved 9 November 2021, from https://www.cdc.gov/spanish/cancer/survivors/patients/treatments.htm. Chatterjee A. The next generation of HPV vaccines: Nonavalent vaccine V503 on the horizon. Expert Review of Vaccines 2014; 13(11):1279-90. Chaturvedi AK, Engels EA, Pfeiffer RM, et al. Human papillomavirus and rising oropharyngeal cancer incidence in the United States. Journal of Clinical Oncology 2011; 29(32):4294–4301. Crosbie EJ, Einstein MH, Franceschi S, Kitchener HC. Human papillomavirus and cervical cancer. Lancet. 2013; 382: 889-899. instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ |
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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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Universidad Antonio Nariño |
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Bioquímica |
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Facultad de Ciencias |
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Bogotá - Circunvalar |
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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Cuervo Soto, Laura InésLópez Quiroz, Angie Vivian118216150192022-04-29T20:52:32Z2022-04-29T20:52:32Z2021-11-26http://repositorio.uan.edu.co/handle/123456789/6370Adang. M; Crickmore, N; & Jurat Fuentes, JL. (2014). Diversity de Bacillus thuringiensis Crystal Toxins and Mechanism of Action. Chapter two In: Insect midgut and insecticidal proteins, volumen 47. p. 39-87.Akiba, T; Abe, Y; Kitada, S; Kusaka, Y; Ito, A; Ichimatsu, T; Katayama, H; Akao, T; Higuchi, K; Mizuki, E; Ohba, M; Kanai, R; & Harata, K. (2009). Crystal Structure of the Parasporin-2 Bacillus thuringiensis Toxin That Recognizes Cancer Cells. Journal of Molecular Biology, 386,121-133.Akiba T, Okumura S. (2017). Parasporins 1 and 2: Their structure and activity. J Invertebr Pathol. 142:44-49. doi: 10.1016/j.jip.2016.10.005.Avignone-Rossa, C. & Mignone, C. (1995) Bacillus thuringiensis Growth and Toxicity. Molecular biotechnology. 4(1), 55-71. doi: 10.1007/BF02907471Bradford Mm. A Rapid And Sensitive Method For The Quantitation Of Microgram Quantities Of Protein Utilizing The Principle Of Protein-Dye Binding. Anal Biochem. 1976, Vol. 7, 72.Bravo A., Gill S.S., Soberón M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon. 2007;49:423–435. doi: 10.1016/j.toxicon.2006.11.022.Centers for Disease Control and Prevention (CDCP) (2021). Tratamientos contra el cáncer. Retrieved 9 November 2021, from https://www.cdc.gov/spanish/cancer/survivors/patients/treatments.htm.Chatterjee A. The next generation of HPV vaccines: Nonavalent vaccine V503 on the horizon. Expert Review of Vaccines 2014; 13(11):1279-90.Chaturvedi AK, Engels EA, Pfeiffer RM, et al. Human papillomavirus and rising oropharyngeal cancer incidence in the United States. Journal of Clinical Oncology 2011; 29(32):4294–4301.Crosbie EJ, Einstein MH, Franceschi S, Kitchener HC. Human papillomavirus and cervical cancer. Lancet. 2013; 382: 889-899.instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/The species of the genus Bacillus are Gram positive bacteria and have a great capacity to form endospores. B. thuringiensis is one of the most studied species for its potential to produce cry proteins with cytotoxic activity against insects. Another group of proteins, parasporins that are formed as parasporal inclusion, show activity against cancer cells, but not against normal cells. 19 parasporins have been identified grouped into 6 families (PS1, PS2, PS3, PS4, PS5 and PS6), with sizes from 30 to 88 kDa, whose activity has been demonstrated on different lines of cancer in humans. Cervical cancer is a type of silent cancer related to the human papillomavirus (HPV), for which existing treatments are not entirely effective. Therefore, the purpose of this study was to obtain parasporal crystals from cell extracts of B. thuringiensis strains, which showed protein values of 8 to 115 μg / mL, a concentration that can be considered optimal for cytotoxicity tests. In addition, bands of proteins related to parasporins were identified by SDS-PAGE electrophoresis, with approximate sizes of 30, 33, 37, 70 and 88 kDa that can correspond to parasporins of all families. These results indicate that these proteins can be tested especially in cervical cancer cells as an alternative for an early and effective treatment.Las especies del género Bacillus son bacterias Gram positivas y presentan gran capacidad de formar endosporas. B. thuringiensis es una de las especies más estudiadas por su potencial para producir proteínas cry con actividad citotóxica frente a insectos. Otro grupo de proteínas, parasporinas que se forman como inclusión parasporal, muestran actividad frente a células de cáncer, más no, sobre células normales. Se han identificado 19 parasporinas agrupadas en 6 familias (PS1, PS2, PS3, PS4, PS5 y PS6), con tamaños de 30 a 88 kDa, cuya actividad ha sido demostrada sobre diferentes líneas de cáncer en humanos. El cáncer de cérvix es un tipo de cáncer silencioso relacionado al virus del papiloma humano (VPH), para el cual los tratamientos existentes no logran ser del todo efectivos. Por ello, el propósito de este estudio fue obtener cristales parasporales de extractos celulares de cepas de B. thuringiensis, los cuales mostraron valores de proteínas de 8 a 115 μg/mL, concentración que puede ser considerada óptima para ensayos de citotoxicidad. Además se identificaron bandas de proteínas relacionadas a parasporinas mediante electroforesis SDS-PAGE, con tamaños aproximados de 30, 33, 37, 70 y 88 kDa que se pueden corresponder a parasporinas de todas las familias. Estos resultados indican que estas proteínas pueden ser probadas especialmente en células de cáncer de cérvix como alternativa para un tratamiento temprano y eficaz.Bioquímico(a)PregradoPresencialInvestigaciónspaUniversidad Antonio NariñoBioquímicaFacultad de CienciasBogotá - CircunvalarParasporinasBacillus thuringiensisVirus del papiloma humano (VPH)CáncerPCR540ParasporinBacillus thuringiensishuman papillomavirusCancerPCRProducción de cristales parasporales de Bacillus thuringiensis con potencial actividad anticancerígenaTrabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85EspecializadaCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.uan.edu.co/bitstreams/60de0fad-c333-4ee2-91fe-ae02a40df4bd/download9868ccc48a14c8d591352b6eaf7f6239MD54ORIGINAL2021_AngieVivianLópezQuiroz_Autorización.pdf2021_AngieVivianLópezQuiroz_Autorización.pdfFormato de autorización de autoresapplication/pdf701722https://repositorio.uan.edu.co/bitstreams/441b8920-3ce0-4fc8-9863-a1c0895e911d/download5a44abcb2d0151a291cf8b5fa2dc7838MD512021_AngieVivianLópezQuiroz.pdf2021_AngieVivianLópezQuiroz.pdfTrabajo de gradoapplication/pdf4023906https://repositorio.uan.edu.co/bitstreams/f6d74b4d-dda4-48a4-8cde-85cdeab804cb/download786282170ee20c88467bfd6ebc5db213MD522021_AngieVivianLópezQuiroz_Acta.pdf2021_AngieVivianLópezQuiroz_Acta.pdfActa de sustentaciónapplication/pdf503194https://repositorio.uan.edu.co/bitstreams/90b6e811-ea7e-46e1-b12a-9f71c344a0a6/downloadf7e97633f06cb635e02fdb2745bd9185MD53123456789/6370oai:repositorio.uan.edu.co:123456789/63702024-10-09 23:22:02.622https://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertorestrictedhttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co |