Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental

Introduction: The dental follicle is a unique tissue composed of a condensation of ectomesenchymal cells that surrounds the dental germ in the early stages of tooth formation. Dental follicle cells can form the 3 types of tissues that make up the periodontium: periodontal ligament, cementum, and alv...

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Autores:
Monzón Sánchez, Lina Mayerli
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2020
Institución:
Universidad Antonio Nariño
Repositorio:
Repositorio UAN
Idioma:
spa
OAI Identifier:
oai:repositorio.uan.edu.co:123456789/2723
Acceso en línea:
http://repositorio.uan.edu.co/handle/123456789/2723
Palabra clave:
Inmunofluorescencia
Citoqueratinas
Foliculo dental
Immunofluorescence
Cytokeratins
Dental follicle
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closedAccess
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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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oai_identifier_str oai:repositorio.uan.edu.co:123456789/2723
network_acronym_str UAntonioN2
network_name_str Repositorio UAN
repository_id_str
dc.title.es_ES.fl_str_mv Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental
title Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental
spellingShingle Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental
Inmunofluorescencia
Citoqueratinas
Foliculo dental
Immunofluorescence
Cytokeratins
Dental follicle
title_short Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental
title_full Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental
title_fullStr Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental
title_full_unstemmed Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental
title_sort Identificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dental
dc.creator.fl_str_mv Monzón Sánchez, Lina Mayerli
dc.contributor.advisor.spa.fl_str_mv Alfonso Rodriguez, Camilo
Jaimes Monroy, Gustavo
dc.contributor.author.spa.fl_str_mv Monzón Sánchez, Lina Mayerli
dc.subject.es_ES.fl_str_mv Inmunofluorescencia
Citoqueratinas
Foliculo dental
topic Inmunofluorescencia
Citoqueratinas
Foliculo dental
Immunofluorescence
Cytokeratins
Dental follicle
dc.subject.keyword.es_ES.fl_str_mv Immunofluorescence
Cytokeratins
Dental follicle
description Introduction: The dental follicle is a unique tissue composed of a condensation of ectomesenchymal cells that surrounds the dental germ in the early stages of tooth formation. Dental follicle cells can form the 3 types of tissues that make up the periodontium: periodontal ligament, cementum, and alveolar bone. Cytokeratins 13 and 19. Result: The results found in this study demonstrated the heterogeneity and cell culture capacity of dental follicle cells. The immunofluorescence assay demonstrated high expression of cytokeratin 19 and moderate cytokeratin 13. Conclusions: The found findings can explain from a basic point of view that this type of cells could differentiate to structurally similar epithelia such as oral mucosa and skin. In this study, the potential of dental follicle stem cells to differentiate into epithelial lineage was confirmed despite their mesenchymal nature and the expression of cytokeratins 13 and 19, being this the one with the highest expression.
publishDate 2020
dc.date.issued.spa.fl_str_mv 2020-05-29
dc.date.accessioned.none.fl_str_mv 2021-03-06T14:43:08Z
dc.date.available.none.fl_str_mv 2021-03-06T14:43:08Z
dc.type.spa.fl_str_mv Trabajo de grado (Pregrado y/o Especialización)
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
format http://purl.org/coar/resource_type/c_7a1f
dc.identifier.uri.none.fl_str_mv http://repositorio.uan.edu.co/handle/123456789/2723
dc.identifier.bibliographicCitation.spa.fl_str_mv 1. Langer R. Advances in Tissue Engineering. EE.UU:; 2016. Report No.: PMS.
2. J. F. development of bilayer and trilayer nanofibrous/microfibrous scaffolds for regenerative medicine. Biomaterials Science. 2013 september;: p. 942–951.
3. Bluteau G. stem cells for tooth engineering. 2008..
4. Alvisi G. Generation of a transgene-free human induced pluripotent stem cell line from oral mucosa epithelial stem cell. elsevier. 2018 Feb 18;: p.4
5. Rao RS. Oral Cytokeratins in Health and Disease. J Contemp Dent Pract review article. 2014;: p. 127-136.
6. Rao RS. Oral Cytokeratins in Health and Disease. J Contemp Dent Pract review article. 2014;: p. 127-136.
7. Ana Iris Verdecia Jiménez 1. Mortalidad por cáncer bucal en pacientes de la provincia Holguín. 2014;: p. 11
8. Anthony L. Neely. The natural history of periodontal disease in humans:risk factors for tooth loss in caries-free subjects receiving no oral health care. journal of clinical periodontology. 2005;: p. 10.
9. Sonoko Tasaki. Th17 cells differentiated with mycelial membranes of Candida albicans prevent oral candidiasis. journals investing in sciencie. 2018 Feb 12;: p. 10.
Swati Singla. Expression of p53, epidermal growth factor receptor, c erbB2 in oral leukoplakias and oral squamous cell carcinomas. Journal of Cancer Research and Therapeutics. 2016;: p. 6.
11. Sila Çagri ISLER. The effects of different restorative materials on periodontopathogens in combined restorative-periodontal treatment. journal of applied oral science. 2017 Aug 21;: p. 9
12. Maurizio S. Tonetti. Xenogenic collagen matrix or autologous connective tissue graft as adjunct to coronally advanced flaps for coverage of multiple adjacent gingival recession: Randomized trial assessing non-inferiority in root coverage and superiority in oral health-related quality of life. journal of clinical periodontology. 2017 octuber 23;: p. 11.
13. Bluteau G. stem cells for tooth engineering. 2009
14. JG R. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. cell. 1975
15. Reid K. Scaffolding: A Broader View. journal of learning disabilities. 1998; volume 31, number 4, july/august pages 386-396.
16. I. Garzon. Expression of epithelial markers by human umbilical cord stem cells. A topographical analysis. elsevier. 2014 september;: p. 1-7.
17. Ingrid Garzón. Wharton’s Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration. AlphaMed Press. 2013 APRIL 3; 2: p. 625– 632.
18. Jakub Suchánek J. Protocols for Dental-Related Stem Cells. Dental Stem Cells: Regenerative Potential. 2016 julio;(pp.27-56).
19. Marks. S. C, Jr. (1976) Tooth eruption and bone resorption: experimental investigation of the ia (osteopetrotic) rat as a model for studying their relationships. Journal of Otal Pathology 5. 149-163
20. Cahill D., Marks, Jr. Tooth eruption: evidence for the central role of the dental follicle Journal of Oral Pathology 1980:9: 189-200
21. Wise GE, Lin F., Fan W. (1992).Culture and characterization of dental follicle cells from rat molars. Cell Tissue Res 267 :483-492
22. Kadkhoda Z., et al., Assessment of human periodontal ligament stem cell surface molecules and wisdom tooth follicle stem cell surface molecules Journal of Craniomaxillofacial Research 2017. 4(2):352-359.
23. Langer R., Vacanti JP Ingeniería de tejidos. Ciencia 1993; 260 (5110): 920 - 926. doi: 10.1126 / science.8493529
24. Shinsuke Ohba, Fumiko Yano, Ung-il Chung,Tissue engineering of bone and cartilage IBMS BoneKEy (2009), 405–419
25. Ratajczak J 1Las propiedades neurovasculares de las células madre dentales y su importancia en la ingeniería de tejidos dentales, Stem Cells Int. 2016; 2016: 9762871. doi: 10.1155 / 2016/9762871.
26. W.Mahfouz S.Elsalmy J.Corcos A.S.Fayed, Fundamentals of bladder tissue engineering, African Journal of UrologyVolume 19, Issue 2, June 2013, Pages 51-57.
27. Péter Windisch, Complex analysis of periodontal regenerative procedures, DMD. PhD THESIS Semmelweis University Budapest 2003. Disponible en: http://phd.semmelweis.hu/mwp/phd_live/vedes/export/windisch.d.pdf
28. Corcoran JP, Ferretti P. Keratin 8 and 18 expression in mesenchymal progenitor cells of regenerating limbs is associated with cell proliferation and differentiation. Dev Dyn. 1997 Dec;210(4):355-70.
29. Kasper M, Karsten U, Stosiek P, Moll R. Distribution of intermediate-filament proteins in the human enamel organ: unusually complex pattern of coexpression of cytokeratin polypeptides and vimentin. Differentiation. 1989 Jun;40(3):207-14.
30. Mizuno N, Shiba H, Mouri Y, Xu W, Kudoh S, Kawaguchi H, Kurihara H.Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins. Cell Biol Int. 2005 Feb;29(2):111-7.
31. Mizuno N, Shiba H, Mouri Y, Xu W, Kudoh S, Kawaguchi H, Kurihara H.Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins. Cell Biol Int. 2005 Feb;29(2):111-7.
32. Sharpe PT.Dental mesenchymal stem cells. Development. 2016 Jul 1;143(13):2273-80.
33. Mizuno N, Shiba H, Mouri Y, Xu W, Kudoh S, Kawaguchi H, Kurihara H.Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins. Cell Biol Int. 2005 Feb;29(2):111-7.
34. Honda MJ, Imaizumi M, Tsuchiya S, Morsczeck C . Dental follicle stem cells and tissue engineering. J Oral Sci. 2010 Dec;52(4):541-52
35. Yao S, Pan F, Prpic V, Wise GE. Differentiation of Stem Cells in the Dental Follicle. Journal of dental research. 2008;87(8):767-771.
36. Navabazam AR, Sadeghian Nodoshan F, Sheikhha MH, Miresmaeili SM, Soleimani M, Fesahat F. Characterization of mesenchymal stem cells from human dental pulp, preapical follicle and periodontal ligament. Iran J Reprod Med. 2013 Mar;11(3):235-42.
37. Brizuela C. C.; Galleguillos, G. S.; Carrión, A. F.; Cabrera, P. C.; Luz, C. P. & Inostroza, S. C. Aislación y caracterización de células madre mesenquimales provenientes de pulpa y folículo dentario humano. Int. J. Morphol., 31(2):739-746, 2013.
38. Amila Brkic, Dental Follicle: Role In Development Of Odontogenic Cysts And Tumours, Journal Of Istanbul University Faculty Of Dentistry, Vol 48, No 1 (2014)
39. Meleti M, van der Waal I, Clinicopathological evaluation of 164 dental follicles and dentigerous cysts with emphasis on the presence of odontogenic epithelium in the connective tissue. The hypothesis of "focal ameloblastoma" Med Oral Patol Oral Cir Bucal 2013 18(1):e60-64
40. Honda MJ, Imaizumi M, Tsuchiya S, et al. Células madre del folículo dental e ingeniería de tejidos. J Oral Sci. 2010; 52: 541-52.
dc.identifier.instname.spa.fl_str_mv instname:Universidad Antonio Nariño
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional UAN
dc.identifier.repourl.spa.fl_str_mv repourl:https://repositorio.uan.edu.co/
url http://repositorio.uan.edu.co/handle/123456789/2723
identifier_str_mv 1. Langer R. Advances in Tissue Engineering. EE.UU:; 2016. Report No.: PMS.
2. J. F. development of bilayer and trilayer nanofibrous/microfibrous scaffolds for regenerative medicine. Biomaterials Science. 2013 september;: p. 942–951.
3. Bluteau G. stem cells for tooth engineering. 2008..
4. Alvisi G. Generation of a transgene-free human induced pluripotent stem cell line from oral mucosa epithelial stem cell. elsevier. 2018 Feb 18;: p.4
5. Rao RS. Oral Cytokeratins in Health and Disease. J Contemp Dent Pract review article. 2014;: p. 127-136.
6. Rao RS. Oral Cytokeratins in Health and Disease. J Contemp Dent Pract review article. 2014;: p. 127-136.
7. Ana Iris Verdecia Jiménez 1. Mortalidad por cáncer bucal en pacientes de la provincia Holguín. 2014;: p. 11
8. Anthony L. Neely. The natural history of periodontal disease in humans:risk factors for tooth loss in caries-free subjects receiving no oral health care. journal of clinical periodontology. 2005;: p. 10.
9. Sonoko Tasaki. Th17 cells differentiated with mycelial membranes of Candida albicans prevent oral candidiasis. journals investing in sciencie. 2018 Feb 12;: p. 10.
Swati Singla. Expression of p53, epidermal growth factor receptor, c erbB2 in oral leukoplakias and oral squamous cell carcinomas. Journal of Cancer Research and Therapeutics. 2016;: p. 6.
11. Sila Çagri ISLER. The effects of different restorative materials on periodontopathogens in combined restorative-periodontal treatment. journal of applied oral science. 2017 Aug 21;: p. 9
12. Maurizio S. Tonetti. Xenogenic collagen matrix or autologous connective tissue graft as adjunct to coronally advanced flaps for coverage of multiple adjacent gingival recession: Randomized trial assessing non-inferiority in root coverage and superiority in oral health-related quality of life. journal of clinical periodontology. 2017 octuber 23;: p. 11.
13. Bluteau G. stem cells for tooth engineering. 2009
14. JG R. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. cell. 1975
15. Reid K. Scaffolding: A Broader View. journal of learning disabilities. 1998; volume 31, number 4, july/august pages 386-396.
16. I. Garzon. Expression of epithelial markers by human umbilical cord stem cells. A topographical analysis. elsevier. 2014 september;: p. 1-7.
17. Ingrid Garzón. Wharton’s Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration. AlphaMed Press. 2013 APRIL 3; 2: p. 625– 632.
18. Jakub Suchánek J. Protocols for Dental-Related Stem Cells. Dental Stem Cells: Regenerative Potential. 2016 julio;(pp.27-56).
19. Marks. S. C, Jr. (1976) Tooth eruption and bone resorption: experimental investigation of the ia (osteopetrotic) rat as a model for studying their relationships. Journal of Otal Pathology 5. 149-163
20. Cahill D., Marks, Jr. Tooth eruption: evidence for the central role of the dental follicle Journal of Oral Pathology 1980:9: 189-200
21. Wise GE, Lin F., Fan W. (1992).Culture and characterization of dental follicle cells from rat molars. Cell Tissue Res 267 :483-492
22. Kadkhoda Z., et al., Assessment of human periodontal ligament stem cell surface molecules and wisdom tooth follicle stem cell surface molecules Journal of Craniomaxillofacial Research 2017. 4(2):352-359.
23. Langer R., Vacanti JP Ingeniería de tejidos. Ciencia 1993; 260 (5110): 920 - 926. doi: 10.1126 / science.8493529
24. Shinsuke Ohba, Fumiko Yano, Ung-il Chung,Tissue engineering of bone and cartilage IBMS BoneKEy (2009), 405–419
25. Ratajczak J 1Las propiedades neurovasculares de las células madre dentales y su importancia en la ingeniería de tejidos dentales, Stem Cells Int. 2016; 2016: 9762871. doi: 10.1155 / 2016/9762871.
26. W.Mahfouz S.Elsalmy J.Corcos A.S.Fayed, Fundamentals of bladder tissue engineering, African Journal of UrologyVolume 19, Issue 2, June 2013, Pages 51-57.
27. Péter Windisch, Complex analysis of periodontal regenerative procedures, DMD. PhD THESIS Semmelweis University Budapest 2003. Disponible en: http://phd.semmelweis.hu/mwp/phd_live/vedes/export/windisch.d.pdf
28. Corcoran JP, Ferretti P. Keratin 8 and 18 expression in mesenchymal progenitor cells of regenerating limbs is associated with cell proliferation and differentiation. Dev Dyn. 1997 Dec;210(4):355-70.
29. Kasper M, Karsten U, Stosiek P, Moll R. Distribution of intermediate-filament proteins in the human enamel organ: unusually complex pattern of coexpression of cytokeratin polypeptides and vimentin. Differentiation. 1989 Jun;40(3):207-14.
30. Mizuno N, Shiba H, Mouri Y, Xu W, Kudoh S, Kawaguchi H, Kurihara H.Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins. Cell Biol Int. 2005 Feb;29(2):111-7.
31. Mizuno N, Shiba H, Mouri Y, Xu W, Kudoh S, Kawaguchi H, Kurihara H.Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins. Cell Biol Int. 2005 Feb;29(2):111-7.
32. Sharpe PT.Dental mesenchymal stem cells. Development. 2016 Jul 1;143(13):2273-80.
33. Mizuno N, Shiba H, Mouri Y, Xu W, Kudoh S, Kawaguchi H, Kurihara H.Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins. Cell Biol Int. 2005 Feb;29(2):111-7.
34. Honda MJ, Imaizumi M, Tsuchiya S, Morsczeck C . Dental follicle stem cells and tissue engineering. J Oral Sci. 2010 Dec;52(4):541-52
35. Yao S, Pan F, Prpic V, Wise GE. Differentiation of Stem Cells in the Dental Follicle. Journal of dental research. 2008;87(8):767-771.
36. Navabazam AR, Sadeghian Nodoshan F, Sheikhha MH, Miresmaeili SM, Soleimani M, Fesahat F. Characterization of mesenchymal stem cells from human dental pulp, preapical follicle and periodontal ligament. Iran J Reprod Med. 2013 Mar;11(3):235-42.
37. Brizuela C. C.; Galleguillos, G. S.; Carrión, A. F.; Cabrera, P. C.; Luz, C. P. & Inostroza, S. C. Aislación y caracterización de células madre mesenquimales provenientes de pulpa y folículo dentario humano. Int. J. Morphol., 31(2):739-746, 2013.
38. Amila Brkic, Dental Follicle: Role In Development Of Odontogenic Cysts And Tumours, Journal Of Istanbul University Faculty Of Dentistry, Vol 48, No 1 (2014)
39. Meleti M, van der Waal I, Clinicopathological evaluation of 164 dental follicles and dentigerous cysts with emphasis on the presence of odontogenic epithelium in the connective tissue. The hypothesis of "focal ameloblastoma" Med Oral Patol Oral Cir Bucal 2013 18(1):e60-64
40. Honda MJ, Imaizumi M, Tsuchiya S, et al. Células madre del folículo dental e ingeniería de tejidos. J Oral Sci. 2010; 52: 541-52.
instname:Universidad Antonio Nariño
reponame:Repositorio Institucional UAN
repourl:https://repositorio.uan.edu.co/
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dc.publisher.spa.fl_str_mv Universidad Antonio Nariño
dc.publisher.program.spa.fl_str_mv Especialización en Periodoncia
dc.publisher.faculty.spa.fl_str_mv Facultad de Odontología
dc.publisher.campus.spa.fl_str_mv Bogotá - Circunvalar
institution Universidad Antonio Nariño
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spelling Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso a solo metadatoshttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbAlfonso Rodriguez, CamiloJaimes Monroy, GustavoMonzón Sánchez, Lina Mayerli2021-03-06T14:43:08Z2021-03-06T14:43:08Z2020-05-29http://repositorio.uan.edu.co/handle/123456789/27231. Langer R. Advances in Tissue Engineering. EE.UU:; 2016. Report No.: PMS.2. J. F. development of bilayer and trilayer nanofibrous/microfibrous scaffolds for regenerative medicine. Biomaterials Science. 2013 september;: p. 942–951.3. Bluteau G. stem cells for tooth engineering. 2008..4. Alvisi G. Generation of a transgene-free human induced pluripotent stem cell line from oral mucosa epithelial stem cell. elsevier. 2018 Feb 18;: p.45. Rao RS. Oral Cytokeratins in Health and Disease. J Contemp Dent Pract review article. 2014;: p. 127-136.6. Rao RS. Oral Cytokeratins in Health and Disease. J Contemp Dent Pract review article. 2014;: p. 127-136.7. Ana Iris Verdecia Jiménez 1. Mortalidad por cáncer bucal en pacientes de la provincia Holguín. 2014;: p. 118. Anthony L. Neely. The natural history of periodontal disease in humans:risk factors for tooth loss in caries-free subjects receiving no oral health care. journal of clinical periodontology. 2005;: p. 10.9. Sonoko Tasaki. Th17 cells differentiated with mycelial membranes of Candida albicans prevent oral candidiasis. journals investing in sciencie. 2018 Feb 12;: p. 10.Swati Singla. Expression of p53, epidermal growth factor receptor, c erbB2 in oral leukoplakias and oral squamous cell carcinomas. Journal of Cancer Research and Therapeutics. 2016;: p. 6.11. Sila Çagri ISLER. The effects of different restorative materials on periodontopathogens in combined restorative-periodontal treatment. journal of applied oral science. 2017 Aug 21;: p. 912. Maurizio S. Tonetti. Xenogenic collagen matrix or autologous connective tissue graft as adjunct to coronally advanced flaps for coverage of multiple adjacent gingival recession: Randomized trial assessing non-inferiority in root coverage and superiority in oral health-related quality of life. journal of clinical periodontology. 2017 octuber 23;: p. 11.13. Bluteau G. stem cells for tooth engineering. 200914. JG R. Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. cell. 197515. Reid K. Scaffolding: A Broader View. journal of learning disabilities. 1998; volume 31, number 4, july/august pages 386-396.16. I. Garzon. Expression of epithelial markers by human umbilical cord stem cells. A topographical analysis. elsevier. 2014 september;: p. 1-7.17. Ingrid Garzón. Wharton’s Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration. AlphaMed Press. 2013 APRIL 3; 2: p. 625– 632.18. Jakub Suchánek J. Protocols for Dental-Related Stem Cells. Dental Stem Cells: Regenerative Potential. 2016 julio;(pp.27-56).19. Marks. S. C, Jr. (1976) Tooth eruption and bone resorption: experimental investigation of the ia (osteopetrotic) rat as a model for studying their relationships. Journal of Otal Pathology 5. 149-16320. Cahill D., Marks, Jr. Tooth eruption: evidence for the central role of the dental follicle Journal of Oral Pathology 1980:9: 189-20021. Wise GE, Lin F., Fan W. (1992).Culture and characterization of dental follicle cells from rat molars. Cell Tissue Res 267 :483-49222. Kadkhoda Z., et al., Assessment of human periodontal ligament stem cell surface molecules and wisdom tooth follicle stem cell surface molecules Journal of Craniomaxillofacial Research 2017. 4(2):352-359.23. Langer R., Vacanti JP Ingeniería de tejidos. Ciencia 1993; 260 (5110): 920 - 926. doi: 10.1126 / science.849352924. Shinsuke Ohba, Fumiko Yano, Ung-il Chung,Tissue engineering of bone and cartilage IBMS BoneKEy (2009), 405–41925. Ratajczak J 1Las propiedades neurovasculares de las células madre dentales y su importancia en la ingeniería de tejidos dentales, Stem Cells Int. 2016; 2016: 9762871. doi: 10.1155 / 2016/9762871.26. W.Mahfouz S.Elsalmy J.Corcos A.S.Fayed, Fundamentals of bladder tissue engineering, African Journal of UrologyVolume 19, Issue 2, June 2013, Pages 51-57.27. Péter Windisch, Complex analysis of periodontal regenerative procedures, DMD. PhD THESIS Semmelweis University Budapest 2003. Disponible en: http://phd.semmelweis.hu/mwp/phd_live/vedes/export/windisch.d.pdf28. Corcoran JP, Ferretti P. Keratin 8 and 18 expression in mesenchymal progenitor cells of regenerating limbs is associated with cell proliferation and differentiation. Dev Dyn. 1997 Dec;210(4):355-70.29. Kasper M, Karsten U, Stosiek P, Moll R. Distribution of intermediate-filament proteins in the human enamel organ: unusually complex pattern of coexpression of cytokeratin polypeptides and vimentin. Differentiation. 1989 Jun;40(3):207-14.30. Mizuno N, Shiba H, Mouri Y, Xu W, Kudoh S, Kawaguchi H, Kurihara H.Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins. Cell Biol Int. 2005 Feb;29(2):111-7.31. Mizuno N, Shiba H, Mouri Y, Xu W, Kudoh S, Kawaguchi H, Kurihara H.Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins. Cell Biol Int. 2005 Feb;29(2):111-7.32. Sharpe PT.Dental mesenchymal stem cells. Development. 2016 Jul 1;143(13):2273-80.33. Mizuno N, Shiba H, Mouri Y, Xu W, Kudoh S, Kawaguchi H, Kurihara H.Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins. Cell Biol Int. 2005 Feb;29(2):111-7.34. Honda MJ, Imaizumi M, Tsuchiya S, Morsczeck C . Dental follicle stem cells and tissue engineering. J Oral Sci. 2010 Dec;52(4):541-5235. Yao S, Pan F, Prpic V, Wise GE. Differentiation of Stem Cells in the Dental Follicle. Journal of dental research. 2008;87(8):767-771.36. Navabazam AR, Sadeghian Nodoshan F, Sheikhha MH, Miresmaeili SM, Soleimani M, Fesahat F. Characterization of mesenchymal stem cells from human dental pulp, preapical follicle and periodontal ligament. Iran J Reprod Med. 2013 Mar;11(3):235-42.37. Brizuela C. C.; Galleguillos, G. S.; Carrión, A. F.; Cabrera, P. C.; Luz, C. P. & Inostroza, S. C. Aislación y caracterización de células madre mesenquimales provenientes de pulpa y folículo dentario humano. Int. J. Morphol., 31(2):739-746, 2013.38. Amila Brkic, Dental Follicle: Role In Development Of Odontogenic Cysts And Tumours, Journal Of Istanbul University Faculty Of Dentistry, Vol 48, No 1 (2014)39. Meleti M, van der Waal I, Clinicopathological evaluation of 164 dental follicles and dentigerous cysts with emphasis on the presence of odontogenic epithelium in the connective tissue. The hypothesis of "focal ameloblastoma" Med Oral Patol Oral Cir Bucal 2013 18(1):e60-6440. Honda MJ, Imaizumi M, Tsuchiya S, et al. Células madre del folículo dental e ingeniería de tejidos. J Oral Sci. 2010; 52: 541-52.instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/Introduction: The dental follicle is a unique tissue composed of a condensation of ectomesenchymal cells that surrounds the dental germ in the early stages of tooth formation. Dental follicle cells can form the 3 types of tissues that make up the periodontium: periodontal ligament, cementum, and alveolar bone. Cytokeratins 13 and 19. Result: The results found in this study demonstrated the heterogeneity and cell culture capacity of dental follicle cells. The immunofluorescence assay demonstrated high expression of cytokeratin 19 and moderate cytokeratin 13. Conclusions: The found findings can explain from a basic point of view that this type of cells could differentiate to structurally similar epithelia such as oral mucosa and skin. In this study, the potential of dental follicle stem cells to differentiate into epithelial lineage was confirmed despite their mesenchymal nature and the expression of cytokeratins 13 and 19, being this the one with the highest expression.Introducción: El folículo dental es un tejido único compuesto por una condensación de células ectomesenquimales que rodea el germen dental en las primeras etapas de la formación del diente. Las células folículo dental pueden formar los 3 tipos de tejidos que constituyen el periodonto: ligamento periodontal, cemento y hueso alveolar. Citoqueratinas 13 y 19.Resultado: Los resultados encontrados en este estudio demostraron la heterogeneidad y la capacidad de cultivo celular de las células de folículo dental. El ensayo por inmunofluorescencia demostró alta expresión de citoqueratina 19 y moderada de citoqueratina 13. Conclusiones: Los hallazgos encontrados pueden explicar desde un punto de vista básico que este tipo de células podrían diferenciarse a epitelios estructuralmente similares como mucosa oral y piel. En este estudio se confirmó el potencial de las células madre del folículo dental , para diferenciarse a linaje epitelial a pesar de su naturaleza mesenquimal y la expresión de las citoqueratinas 13 y 19 siendo esta la de mayor expresión.Especialista en PeriodonciaEspecializaciónPresencialspaUniversidad Antonio NariñoEspecialización en PeriodonciaFacultad de OdontologíaBogotá - CircunvalarInmunofluorescenciaCitoqueratinasFoliculo dentalImmunofluorescenceCytokeratinsDental follicleIdentificación por inmunofluorescencia de citoqueratinas 13 y 19 en células mensequimales del folículo dentalTrabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85ORIGINAL2020LinaMayerliMonzónSanchez.pdf2020LinaMayerliMonzónSanchez.pdfTRABAJO DE GRADOapplication/pdf832920https://repositorio.uan.edu.co/bitstreams/5e2080ac-c974-4b22-9e9e-a51d4dd4e660/downloadb9ee0cba7bc4b1fa58ffdb433d602168MD512020AutorizacióndeAutores.pdf2020AutorizacióndeAutores.pdfAUTORIZACIÓNapplication/pdf336371https://repositorio.uan.edu.co/bitstreams/69643479-8ce8-43e9-a75b-0da36aa78c39/download8be049f3111602c4952fb68e6d7045beMD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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