Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa)
The presence and persistence of pharmaceuticals in the food chain, especially in food crops, can have negative effects on human health and the environment. Acetaminophen in particular is a significant environmental pollutant due to its widespread use. The objective of this work was to determine the...
- Autores:
-
Paez Almanza, Valentina
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2022
- Institución:
- Universidad Antonio Nariño
- Repositorio:
- Repositorio UAN
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uan.edu.co:123456789/7383
- Acceso en línea:
- http://repositorio.uan.edu.co/handle/123456789/7383
- Palabra clave:
- Degradación
bacterias endófitas
paracetamol, lechuga
572
Degradation
endophytic bacteria
acetaminophen, lettuce.
- Rights
- openAccess
- License
- Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa) |
| title |
Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa) |
| spellingShingle |
Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa) Degradación bacterias endófitas paracetamol, lechuga 572 Degradation endophytic bacteria acetaminophen, lettuce. |
| title_short |
Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa) |
| title_full |
Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa) |
| title_fullStr |
Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa) |
| title_full_unstemmed |
Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa) |
| title_sort |
Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa) |
| dc.creator.fl_str_mv |
Paez Almanza, Valentina |
| dc.contributor.advisor.spa.fl_str_mv |
Martinez Pachón, Diana Vanegas Guerrero, Javier |
| dc.contributor.author.spa.fl_str_mv |
Paez Almanza, Valentina |
| dc.subject.es_ES.fl_str_mv |
Degradación bacterias endófitas paracetamol, lechuga |
| topic |
Degradación bacterias endófitas paracetamol, lechuga 572 Degradation endophytic bacteria acetaminophen, lettuce. |
| dc.subject.ddc.es_ES.fl_str_mv |
572 |
| dc.subject.keyword.es_ES.fl_str_mv |
Degradation endophytic bacteria acetaminophen, lettuce. |
| description |
The presence and persistence of pharmaceuticals in the food chain, especially in food crops, can have negative effects on human health and the environment. Acetaminophen in particular is a significant environmental pollutant due to its widespread use. The objective of this work was to determine the degradation of acetaminophen (ACE) by endophytic bacteria from lettuce from 3 municipalities of the Sabana de Bogotá (Cota, Mosquera and Funza). For this purpose, lettuce endophytes were selected in minimal salt medium containing acetaminophen, drug degradation was performed by HPLC, and bacteria were identified by 16s rRNA gene sequencing. The bacteria Klebsiella sp L1cp and Klebsiella pneumoniae L2cp use acetaminophen as a carbon and energy source for their growth. Strains L1cp and L2cp degraded 20% and 70% of acetamiphone at 50 mg/l in 60 h. Glucose addition affected ACE degradation, with L1cp degradation increased by 33% and L2cp degradation decreased by 53%. These results suggest the potential of lettuce endophytic bacteria to degrade acetaminophen and a possible mechanism of protection or detoxification of exogenous substances in plants. |
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2022 |
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2022-12-13 |
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2023-01-17T16:52:57Z |
| dc.date.available.none.fl_str_mv |
2023-01-17T16:52:57Z |
| dc.type.spa.fl_str_mv |
Trabajo de grado (Pregrado y/o Especialización) |
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http://purl.org/coar/resource_type/c_7a1f |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_7a1f |
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http://repositorio.uan.edu.co/handle/123456789/7383 |
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Adegoke, A. A., Amoah, I. D., Stenström, T. A., Verbyla, M. E., & Mihelcic, J. R. (2018). Epidemiological evidence and health risks associated with agricultural reuse of partially treated and untreated wastewater: a review. Frontiers in public health, 6, 337. https://doi.org/10.3389/fpubh.2018.00337 Aissaoui, S., Ouled-Haddar, H., Sifour, M., Harrouche, K., & Sghaier, H. (2017). Metabolic and co-metabolic transformation of diclofenac by Enterobacter hormaechei D15 isolated from activated sludge. Current microbiology, 74(3), 381-388. https://doi.org/10.1007/s00284-016-1190-x Akay, C., & Tezel, U. (2020). Biotransformation of Acetaminophen by intact cells and crude enzymes of bacteria: A comparative study and modelling. Science of the total environment, 703, 134990. https://doi.org/10.1016/j.scitotenv.2019.134990 Akin-Ajani, O. D., Odeku, O. A., & Olumakinde-Oni, O. (2020). Evaluation of the mechanical and release properties of lactose and microcrystalline cellulose and their binary mixtures as directly compressible excipients in paracetamol tablets. Journal of Excipients and Food Chemicals, 11(2), 42-52. Ashraf, S., Afzal, M., Naveed, M., Shahid, M., & Ahmad Zahir, Z. (2018). Endophytic bacteria enhance remediation of tannery effluent in constructed wetlands vegetated with Leptochloa fusca. International Journal of Phytoremediation, 20(2), 121-128. DOI: 10.1080/15226514.2017.1337072. Badar, Z., Shanableh, A., El-Keblawy, A., Mosa, K. A., Semerjian, L., Mutery, A. A., ... & Semreen, M. H. (2022). Assessment of Uptake, Accumulation and Degradation of Paracetamol in Spinach (Spinacia oleracea L.) under Controlled Laboratory Conditions. Plants, 11(13), 1626. https://doi.org/10.3390/plants11131626 Bai, Y., Cui, Z., Su, R., & Qu, K. (2018). Influence of DOM components, salinity, pH, nitrate, and bicarbonate on the indirect photodegradation of acetaminophen in simulated coastal waters. Chemosphere, 205, 108-117. https://doi.org/10.1016/j.chemosphere.2018.04.087 Banco mundial (2017). Agua en la agricultura. https://www.bancomundial.org/es/topic/water-in-agriculture Bittsánszky, A., Gullner, G., Gyulai, G., & Komives, T. (2011). A case study: uptake and accumulation of persistent organic pollutants in Cucurbitaceae species. In Organic xenobiotics and plants (pp. 77-85). Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9852-8_4 Botero-Coy, A. M., Martínez-Pachón, D., Boix, C., Rincón, R. J., Castillo, N., Arias-Marín, L. P., ... & Hernandez, F. (2018). An investigation into the occurrence and removal of pharmaceuticals in Colombian wastewater. Science of the Total Environment, 642, 842-853. https://doi.org/10.1016/j.scitotenv.2018.06.088 |
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Adegoke, A. A., Amoah, I. D., Stenström, T. A., Verbyla, M. E., & Mihelcic, J. R. (2018). Epidemiological evidence and health risks associated with agricultural reuse of partially treated and untreated wastewater: a review. Frontiers in public health, 6, 337. https://doi.org/10.3389/fpubh.2018.00337 Aissaoui, S., Ouled-Haddar, H., Sifour, M., Harrouche, K., & Sghaier, H. (2017). Metabolic and co-metabolic transformation of diclofenac by Enterobacter hormaechei D15 isolated from activated sludge. Current microbiology, 74(3), 381-388. https://doi.org/10.1007/s00284-016-1190-x Akay, C., & Tezel, U. (2020). Biotransformation of Acetaminophen by intact cells and crude enzymes of bacteria: A comparative study and modelling. Science of the total environment, 703, 134990. https://doi.org/10.1016/j.scitotenv.2019.134990 Akin-Ajani, O. D., Odeku, O. A., & Olumakinde-Oni, O. (2020). Evaluation of the mechanical and release properties of lactose and microcrystalline cellulose and their binary mixtures as directly compressible excipients in paracetamol tablets. Journal of Excipients and Food Chemicals, 11(2), 42-52. Ashraf, S., Afzal, M., Naveed, M., Shahid, M., & Ahmad Zahir, Z. (2018). Endophytic bacteria enhance remediation of tannery effluent in constructed wetlands vegetated with Leptochloa fusca. International Journal of Phytoremediation, 20(2), 121-128. DOI: 10.1080/15226514.2017.1337072. Badar, Z., Shanableh, A., El-Keblawy, A., Mosa, K. A., Semerjian, L., Mutery, A. A., ... & Semreen, M. H. (2022). Assessment of Uptake, Accumulation and Degradation of Paracetamol in Spinach (Spinacia oleracea L.) under Controlled Laboratory Conditions. Plants, 11(13), 1626. https://doi.org/10.3390/plants11131626 Bai, Y., Cui, Z., Su, R., & Qu, K. (2018). Influence of DOM components, salinity, pH, nitrate, and bicarbonate on the indirect photodegradation of acetaminophen in simulated coastal waters. Chemosphere, 205, 108-117. https://doi.org/10.1016/j.chemosphere.2018.04.087 Banco mundial (2017). Agua en la agricultura. https://www.bancomundial.org/es/topic/water-in-agriculture Bittsánszky, A., Gullner, G., Gyulai, G., & Komives, T. (2011). A case study: uptake and accumulation of persistent organic pollutants in Cucurbitaceae species. In Organic xenobiotics and plants (pp. 77-85). Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9852-8_4 Botero-Coy, A. M., Martínez-Pachón, D., Boix, C., Rincón, R. J., Castillo, N., Arias-Marín, L. P., ... & Hernandez, F. (2018). An investigation into the occurrence and removal of pharmaceuticals in Colombian wastewater. Science of the Total Environment, 642, 842-853. https://doi.org/10.1016/j.scitotenv.2018.06.088 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ |
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Universidad Antonio Nariño |
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Facultad de Ciencias |
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Bogotá - Circunvalar |
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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Martinez Pachón, DianaVanegas Guerrero, JavierPaez Almanza, Valentina118218190192023-01-17T16:52:57Z2023-01-17T16:52:57Z2022-12-13http://repositorio.uan.edu.co/handle/123456789/7383Adegoke, A. A., Amoah, I. D., Stenström, T. A., Verbyla, M. E., & Mihelcic, J. R. (2018). Epidemiological evidence and health risks associated with agricultural reuse of partially treated and untreated wastewater: a review. Frontiers in public health, 6, 337. https://doi.org/10.3389/fpubh.2018.00337Aissaoui, S., Ouled-Haddar, H., Sifour, M., Harrouche, K., & Sghaier, H. (2017). Metabolic and co-metabolic transformation of diclofenac by Enterobacter hormaechei D15 isolated from activated sludge. Current microbiology, 74(3), 381-388. https://doi.org/10.1007/s00284-016-1190-xAkay, C., & Tezel, U. (2020). Biotransformation of Acetaminophen by intact cells and crude enzymes of bacteria: A comparative study and modelling. Science of the total environment, 703, 134990. https://doi.org/10.1016/j.scitotenv.2019.134990Akin-Ajani, O. D., Odeku, O. A., & Olumakinde-Oni, O. (2020). Evaluation of the mechanical and release properties of lactose and microcrystalline cellulose and their binary mixtures as directly compressible excipients in paracetamol tablets. Journal of Excipients and Food Chemicals, 11(2), 42-52.Ashraf, S., Afzal, M., Naveed, M., Shahid, M., & Ahmad Zahir, Z. (2018). Endophytic bacteria enhance remediation of tannery effluent in constructed wetlands vegetated with Leptochloa fusca. International Journal of Phytoremediation, 20(2), 121-128. DOI: 10.1080/15226514.2017.1337072.Badar, Z., Shanableh, A., El-Keblawy, A., Mosa, K. A., Semerjian, L., Mutery, A. A., ... & Semreen, M. H. (2022). Assessment of Uptake, Accumulation and Degradation of Paracetamol in Spinach (Spinacia oleracea L.) under Controlled Laboratory Conditions. Plants, 11(13), 1626. https://doi.org/10.3390/plants11131626Bai, Y., Cui, Z., Su, R., & Qu, K. (2018). Influence of DOM components, salinity, pH, nitrate, and bicarbonate on the indirect photodegradation of acetaminophen in simulated coastal waters. Chemosphere, 205, 108-117. https://doi.org/10.1016/j.chemosphere.2018.04.087Banco mundial (2017). Agua en la agricultura. https://www.bancomundial.org/es/topic/water-in-agricultureBittsánszky, A., Gullner, G., Gyulai, G., & Komives, T. (2011). A case study: uptake and accumulation of persistent organic pollutants in Cucurbitaceae species. In Organic xenobiotics and plants (pp. 77-85). Springer, Dordrecht. https://doi.org/10.1007/978-90-481-9852-8_4Botero-Coy, A. M., Martínez-Pachón, D., Boix, C., Rincón, R. J., Castillo, N., Arias-Marín, L. P., ... & Hernandez, F. (2018). An investigation into the occurrence and removal of pharmaceuticals in Colombian wastewater. Science of the Total Environment, 642, 842-853. https://doi.org/10.1016/j.scitotenv.2018.06.088instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/The presence and persistence of pharmaceuticals in the food chain, especially in food crops, can have negative effects on human health and the environment. Acetaminophen in particular is a significant environmental pollutant due to its widespread use. The objective of this work was to determine the degradation of acetaminophen (ACE) by endophytic bacteria from lettuce from 3 municipalities of the Sabana de Bogotá (Cota, Mosquera and Funza). For this purpose, lettuce endophytes were selected in minimal salt medium containing acetaminophen, drug degradation was performed by HPLC, and bacteria were identified by 16s rRNA gene sequencing. The bacteria Klebsiella sp L1cp and Klebsiella pneumoniae L2cp use acetaminophen as a carbon and energy source for their growth. Strains L1cp and L2cp degraded 20% and 70% of acetamiphone at 50 mg/l in 60 h. Glucose addition affected ACE degradation, with L1cp degradation increased by 33% and L2cp degradation decreased by 53%. These results suggest the potential of lettuce endophytic bacteria to degrade acetaminophen and a possible mechanism of protection or detoxification of exogenous substances in plants.La presencia y persistencia de productos farmacéuticos en la cadena alimentaria, especialmente en los cultivos alimentarios, puede tener efectos negativos sobre la salud humana y el medio ambiente. El acetaminofén en particular es un contaminante ambiental importante debido a su uso generalizado. El objetivo de este trabajo fue determinar la degradación del acetaminofén (ACE) por bacterias endófitas provenientes de lechuga de 3 municipios de la Sabana de Bogotá (Cota, Mosquera y Funza). Para este propósito, los endófitos de lechuga se seleccionaron en medio mínimo de sal que contenía acetaminofén, la degradación del fármaco se realizó por HPLC y las bacterias se identificaron por secuenciación del gen 16s rRNA. Las bacterias Klebsiella sp L1cp y Klebsiella pneumoniae L2cp utilizan acetaminofén como fuente de carbono y energía para su crecimiento. Las cepas L1cp y L2cp degradaron el 20 % y el 70 % del acetaminofen a 50 mg/l en 60 h. La adición de glucosa afectó la degradación de la ACE, ya que la degradación de L1cp aumentó en un 33 % y la de L2cp disminuyó en un 53 %. Estos resultados sugieren el potencial de las bacterias endófitas de la lechuga para degradar el acetaminofén y un posible mecanismo de protección o detoxificación de sustancias exógenas en las plantas.Bioquímico(a)PregradoPresencialInvestigaciónspaUniversidad Antonio NariñoBioquímicaFacultad de CienciasBogotá - CircunvalarDegradaciónbacterias endófitasparacetamol, lechuga572Degradationendophytic bacteriaacetaminophen, lettuce.Degradación de Acetaminofén en matrices acuosas por medio de bacterias endófitas provenientes del cultivo de lechuga (Lactuca sativa)Trabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85EspecializadaORIGINAL2023_ ValentinaPaezAlmanza_Acta.pdf2023_ ValentinaPaezAlmanza_Acta.pdfapplication/pdf131754https://repositorio.uan.edu.co/bitstreams/7ee5f3f0-aa15-4346-b8bb-80024cd83937/downloadf08e47d3aef3cfb45c49fed6a69e8a7bMD512023_ValentinaPaezAlmanza.pdf2023_ValentinaPaezAlmanza.pdfapplication/pdf805522https://repositorio.uan.edu.co/bitstreams/70e6230c-3c58-4b3c-bd6e-c9a9093018d6/download4970229d7c925265d596a1409e3ea634MD522023_ ValentinaPaezAlmanza_Autorizacion.pdf2023_ ValentinaPaezAlmanza_Autorizacion.pdfapplication/pdf654695https://repositorio.uan.edu.co/bitstreams/88694828-9e8d-46cc-9c3d-2c7af44b9cdd/download2fb80feaec2908825cf9aa83a3ff6a1dMD53123456789/7383oai:repositorio.uan.edu.co:123456789/73832024-10-09 22:46:16.795https://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertoopen.accesshttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co |