Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto.

The stem cells of the apical papilla have excellent properties of differentiation and great potential for use in tissue engineering protocols, without However, the potential for epithelial differentiation has not been explored with this cell type. The objective is to analyze the expression of cytoke...

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Autores:: Rodriguez Reyes, Diego Andrés
Huertas Torres, Christian Andrés
Vera Tenjo, Julián Andrés

Tipo de recurso:: Trabajo de grado de pregrado

Fecha de publicación:: 2020

Institución:: Universidad Antonio Nariño

Repositorio:: Repositorio UAN

Idioma:: spa

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dc.title.es_ES.fl_str_mv	Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto.
title	Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto.
spellingShingle	Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto. citoqueratinas 8, 13, 19. desmogleina desmoplaquina papila apical cytokeratin 8, 13, 19 desmoglein desmoplakin apical papilla
title_short	Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto.
title_full	Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto.
title_fullStr	Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto.
title_full_unstemmed	Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto.
title_sort	Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto.
dc.creator.fl_str_mv	Rodriguez Reyes, Diego Andrés Huertas Torres, Christian Andrés Vera Tenjo, Julián Andrés
dc.contributor.advisor.spa.fl_str_mv	Alfonso Rodriguez, Camilo Jaimes Monroy, Gustavo
dc.contributor.author.spa.fl_str_mv	Rodriguez Reyes, Diego Andrés Huertas Torres, Christian Andrés Vera Tenjo, Julián Andrés
dc.subject.es_ES.fl_str_mv	citoqueratinas 8, 13, 19. desmogleina desmoplaquina papila apical
topic	citoqueratinas 8, 13, 19. desmogleina desmoplaquina papila apical cytokeratin 8, 13, 19 desmoglein desmoplakin apical papilla
dc.subject.keyword.es_ES.fl_str_mv	cytokeratin 8, 13, 19 desmoglein desmoplakin apical papilla
description	The stem cells of the apical papilla have excellent properties of differentiation and great potential for use in tissue engineering protocols, without However, the potential for epithelial differentiation has not been explored with this cell type. The objective is to analyze the expression of cytokeratins 8, 13, and 19, desmoplaquine and desmoglein by immunofluorescence. Materials and Methods: Apical papilla tissue cell cultures were performed using Explant technique of healthy donor patients. Characterization was performed cell by flow cytometry, was analyzed by immunofluorescence the expression of cytokeratins 8, 13 and 19, desmoplakine and desmoglein. Results: The cells of the apical papilla expressed cytokeratin 8, 13 and 19 desmoplaquine and desmoglein, observed through the immunofluorescence technique, which was applied to the cells of the fourth pass that demonstrated a cell viability of 86.76% by means of cytometry of flow and trypan blue. Conclusions: The stem cells of the apical papilla express epithelial markers and support the idea that they could be used as an alternative source to develop artificial oral mucosa protocols.
publishDate	2020
dc.date.issued.spa.fl_str_mv	2020-05-29
dc.date.accessioned.none.fl_str_mv	2021-03-06T14:54:20Z
dc.date.available.none.fl_str_mv	2021-03-06T14:54:20Z
dc.type.spa.fl_str_mv	Trabajo de grado (Pregrado y/o Especialización)
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dc.identifier.uri.none.fl_str_mv	http://repositorio.uan.edu.co/handle/123456789/2725
dc.identifier.bibliographicCitation.spa.fl_str_mv	1- Kim R, Fasi A, Feinberg S. Soft tissue engineering in craniomaxillofacial surgery. Ann Maxillofac Surg. 2014; 4 (1): 4-8 2- Bluteau G, Luder HU, De Bari C, Mitsiadis TA. Stem cells for tooth engineering. Eur Cell Mater. 2008; 16:1-9 3- Tonetti MS, Cortellini P, Pellegrini G1, Nieri M, Bonaccini D, Allegri M, et al. Xenogenic collagen matrix or autologous connective tissue graft as adjunct to coronally advanced flaps for coverage of multiple adjacent gingival recession: Randomized trial assessing non-inferiority in root coverage and superiority in oral health-related quality of life. J Clin Periodontol. 2018; 45(1):78-88 4- Alfonso Rodriguez Camilo,Generación de Mucosa Oral Artificial Por Ingeniería Tisular,Granada España,Universidad de Granada,2014. 5- Karaöz E, Demircan PC, Sağlam O, Aksoy A, Kaymaz F, Duruksu G. Human dental pulp stem cells demonstrate better neural and epithelial stem cell properties than bone marrow-derived mesenchymal stem cells.Histochem Cell Biol. 2011; 136(4): 455-473. 6- Rao RS, Patil S, Ganavi BS. Oral Cytokeratins in Health and Disease. J Contemp Dent Pract. 2014; 15(1):127-36. 7- Holmstrup P, Plemons J, Meyle J.Non–plaque‐ induced gingival diseases.J Periodontol. 2018; 89(1):28-45 8- Sanchez-Quevedo M, Alaminos M, Capitan LM, Moreu G, Garzon I, Crespo PV, et al. Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering. Histol Histopathol. 2007; 22(6):631-40. 9- Bluteau G, Luder HU, De Bari C, Mitsiadis TA. Stem cells for tooth engineering. Eur Cell Mater. 2008; 16:1-9 10- Garzon I, Alfonso-Rodríguez CA, Martínez-Gómez C, Carriel V, Martin-Piedra MA, Fernández-Valadés R, et al. Expression of epithelial markers by human umbilical cord stem cells. A topographical analysis. Placenta. 2014; 35(12):994- 1000. 11- Garzón I, Miyake J, González-Andrades M, Carmona R, Carda C, SánchezQuevedo Mdel C, et al. Wharton’s Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration. Stem Cells Transl Med. 2013; 2(8):625-32 12- Martin-Piedra M, Garzon I, Oliveira A, Alfonso-Rodriguez CA, Carriel V, Scionti G, et al. Cell viability and proliferation capability of long-term human dental pulp stem cell cultures. Cytotherapy. 2014; 16(2):266-77 13- Martin-Piedra M, Garzon I, Oliveira AC, Alfonso-Rodríguez CA, SánchezQuevedo M, Campos A, et al. Average cell viability levels of human dental pulp stem cells: an accurate combinatorial index for quality control in tissue engineering. Cytotherapy. 2013; 15(4):507-18 14- Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4): 315-17. 15- Sonoyama W, Liu Y, Yamaza T, Tuan RS, Wang S, Shi S, et al. Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study. J Endod. 2008; 34(2):166-71. 16- Bakopoulou A, About I. Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application. Stem Cells Int. 2016; 2016:1- 20 17- Pang X, Zhuang Y, Li Z, Jing S, Cai Q, Zhang F, et al. Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways. Stem Cells Int. 2020; 2020:1-13. 18- Marañés C, Liceras E, Alaminos M, Fernández R, Ruiz A, Garzon I, et al. Generación de un sustituto de mucosa oral humana y comprobación de su viabilidad mediante ingeniería tisular. Cir Pediatr 2011; 24: 13-18. 19- Elias P, Matsuyoshi N, Wu H, Lin C, Wang Z, Brown B, et al. Desmoglein Isoform Distribution Affects Stratum Corneum Structure and Function. J Cell Biol. 2001; 153(2): 243-50. 20- Mahoney M, Sadowski S, Brennan D, Pikander P, Saukko P, Wahl J, et al. Compound heterozygous desmoplakin mutations result in a phenotype with a combination of myocardial, skin, hair, and enamel abnormalities. J Invest Dermatol. 2010; 130(4):968-78 21- Doğan A, Demirci S, Şahin F. In vitro differentiation of human tooth germ stem cells into endothelial- and epithelial-like cells. Cell Biol Int. 2015; 39(1):94-103.
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url	http://repositorio.uan.edu.co/handle/123456789/2725
identifier_str_mv	1- Kim R, Fasi A, Feinberg S. Soft tissue engineering in craniomaxillofacial surgery. Ann Maxillofac Surg. 2014; 4 (1): 4-8 2- Bluteau G, Luder HU, De Bari C, Mitsiadis TA. Stem cells for tooth engineering. Eur Cell Mater. 2008; 16:1-9 3- Tonetti MS, Cortellini P, Pellegrini G1, Nieri M, Bonaccini D, Allegri M, et al. Xenogenic collagen matrix or autologous connective tissue graft as adjunct to coronally advanced flaps for coverage of multiple adjacent gingival recession: Randomized trial assessing non-inferiority in root coverage and superiority in oral health-related quality of life. J Clin Periodontol. 2018; 45(1):78-88 4- Alfonso Rodriguez Camilo,Generación de Mucosa Oral Artificial Por Ingeniería Tisular,Granada España,Universidad de Granada,2014. 5- Karaöz E, Demircan PC, Sağlam O, Aksoy A, Kaymaz F, Duruksu G. Human dental pulp stem cells demonstrate better neural and epithelial stem cell properties than bone marrow-derived mesenchymal stem cells.Histochem Cell Biol. 2011; 136(4): 455-473. 6- Rao RS, Patil S, Ganavi BS. Oral Cytokeratins in Health and Disease. J Contemp Dent Pract. 2014; 15(1):127-36. 7- Holmstrup P, Plemons J, Meyle J.Non–plaque‐ induced gingival diseases.J Periodontol. 2018; 89(1):28-45 8- Sanchez-Quevedo M, Alaminos M, Capitan LM, Moreu G, Garzon I, Crespo PV, et al. Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering. Histol Histopathol. 2007; 22(6):631-40. 9- Bluteau G, Luder HU, De Bari C, Mitsiadis TA. Stem cells for tooth engineering. Eur Cell Mater. 2008; 16:1-9 10- Garzon I, Alfonso-Rodríguez CA, Martínez-Gómez C, Carriel V, Martin-Piedra MA, Fernández-Valadés R, et al. Expression of epithelial markers by human umbilical cord stem cells. A topographical analysis. Placenta. 2014; 35(12):994- 1000. 11- Garzón I, Miyake J, González-Andrades M, Carmona R, Carda C, SánchezQuevedo Mdel C, et al. Wharton’s Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration. Stem Cells Transl Med. 2013; 2(8):625-32 12- Martin-Piedra M, Garzon I, Oliveira A, Alfonso-Rodriguez CA, Carriel V, Scionti G, et al. Cell viability and proliferation capability of long-term human dental pulp stem cell cultures. Cytotherapy. 2014; 16(2):266-77 13- Martin-Piedra M, Garzon I, Oliveira AC, Alfonso-Rodríguez CA, SánchezQuevedo M, Campos A, et al. Average cell viability levels of human dental pulp stem cells: an accurate combinatorial index for quality control in tissue engineering. Cytotherapy. 2013; 15(4):507-18 14- Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4): 315-17. 15- Sonoyama W, Liu Y, Yamaza T, Tuan RS, Wang S, Shi S, et al. Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study. J Endod. 2008; 34(2):166-71. 16- Bakopoulou A, About I. Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application. Stem Cells Int. 2016; 2016:1- 20 17- Pang X, Zhuang Y, Li Z, Jing S, Cai Q, Zhang F, et al. Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways. Stem Cells Int. 2020; 2020:1-13. 18- Marañés C, Liceras E, Alaminos M, Fernández R, Ruiz A, Garzon I, et al. Generación de un sustituto de mucosa oral humana y comprobación de su viabilidad mediante ingeniería tisular. Cir Pediatr 2011; 24: 13-18. 19- Elias P, Matsuyoshi N, Wu H, Lin C, Wang Z, Brown B, et al. Desmoglein Isoform Distribution Affects Stratum Corneum Structure and Function. J Cell Biol. 2001; 153(2): 243-50. 20- Mahoney M, Sadowski S, Brennan D, Pikander P, Saukko P, Wahl J, et al. Compound heterozygous desmoplakin mutations result in a phenotype with a combination of myocardial, skin, hair, and enamel abnormalities. J Invest Dermatol. 2010; 130(4):968-78 21- Doğan A, Demirci S, Şahin F. In vitro differentiation of human tooth germ stem cells into endothelial- and epithelial-like cells. Cell Biol Int. 2015; 39(1):94-103. instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.rights.none.fl_str_mv	Acceso a solo metadatos
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dc.publisher.spa.fl_str_mv	Universidad Antonio Nariño
dc.publisher.program.spa.fl_str_mv	Especialización en Periodoncia
dc.publisher.faculty.spa.fl_str_mv	Facultad de Odontología
dc.publisher.campus.spa.fl_str_mv	Bogotá - Circunvalar
institution	Universidad Antonio Nariño
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spelling	Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso a solo metadatoshttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/closedAccesshttp://purl.org/coar/access_right/c_14cbAlfonso Rodriguez, CamiloJaimes Monroy, GustavoRodriguez Reyes, Diego AndrésHuertas Torres, Christian AndrésVera Tenjo, Julián Andrés2021-03-06T14:54:20Z2021-03-06T14:54:20Z2020-05-29http://repositorio.uan.edu.co/handle/123456789/27251- Kim R, Fasi A, Feinberg S. Soft tissue engineering in craniomaxillofacial surgery. Ann Maxillofac Surg. 2014; 4 (1): 4-82- Bluteau G, Luder HU, De Bari C, Mitsiadis TA. Stem cells for tooth engineering. Eur Cell Mater. 2008; 16:1-93- Tonetti MS, Cortellini P, Pellegrini G1, Nieri M, Bonaccini D, Allegri M, et al. Xenogenic collagen matrix or autologous connective tissue graft as adjunct to coronally advanced flaps for coverage of multiple adjacent gingival recession: Randomized trial assessing non-inferiority in root coverage and superiority in oral health-related quality of life. J Clin Periodontol. 2018; 45(1):78-884- Alfonso Rodriguez Camilo,Generación de Mucosa Oral Artificial Por Ingeniería Tisular,Granada España,Universidad de Granada,2014.5- Karaöz E, Demircan PC, Sağlam O, Aksoy A, Kaymaz F, Duruksu G. Human dental pulp stem cells demonstrate better neural and epithelial stem cell properties than bone marrow-derived mesenchymal stem cells.Histochem Cell Biol. 2011; 136(4): 455-473.6- Rao RS, Patil S, Ganavi BS. Oral Cytokeratins in Health and Disease. J Contemp Dent Pract. 2014; 15(1):127-36.7- Holmstrup P, Plemons J, Meyle J.Non–plaque‐ induced gingival diseases.J Periodontol. 2018; 89(1):28-458- Sanchez-Quevedo M, Alaminos M, Capitan LM, Moreu G, Garzon I, Crespo PV, et al. Histological and histochemical evaluation of human oral mucosa constructs developed by tissue engineering. Histol Histopathol. 2007; 22(6):631-40.9- Bluteau G, Luder HU, De Bari C, Mitsiadis TA. Stem cells for tooth engineering. Eur Cell Mater. 2008; 16:1-910- Garzon I, Alfonso-Rodríguez CA, Martínez-Gómez C, Carriel V, Martin-Piedra MA, Fernández-Valadés R, et al. Expression of epithelial markers by human umbilical cord stem cells. A topographical analysis. Placenta. 2014; 35(12):994- 1000.11- Garzón I, Miyake J, González-Andrades M, Carmona R, Carda C, SánchezQuevedo Mdel C, et al. Wharton’s Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration. Stem Cells Transl Med. 2013; 2(8):625-3212- Martin-Piedra M, Garzon I, Oliveira A, Alfonso-Rodriguez CA, Carriel V, Scionti G, et al. Cell viability and proliferation capability of long-term human dental pulp stem cell cultures. Cytotherapy. 2014; 16(2):266-7713- Martin-Piedra M, Garzon I, Oliveira AC, Alfonso-Rodríguez CA, SánchezQuevedo M, Campos A, et al. Average cell viability levels of human dental pulp stem cells: an accurate combinatorial index for quality control in tissue engineering. Cytotherapy. 2013; 15(4):507-1814- Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4): 315-17.15- Sonoyama W, Liu Y, Yamaza T, Tuan RS, Wang S, Shi S, et al. Characterization of the apical papilla and its residing stem cells from human immature permanent teeth: a pilot study. J Endod. 2008; 34(2):166-71.16- Bakopoulou A, About I. Stem Cells of Dental Origin: Current Research Trends and Key Milestones towards Clinical Application. Stem Cells Int. 2016; 2016:1- 2017- Pang X, Zhuang Y, Li Z, Jing S, Cai Q, Zhang F, et al. Intermittent Administration of Parathyroid Hormone Enhances Odonto/Osteogenic Differentiation of Stem Cells from the Apical Papilla via JNK and P38 MAPK Pathways. Stem Cells Int. 2020; 2020:1-13.18- Marañés C, Liceras E, Alaminos M, Fernández R, Ruiz A, Garzon I, et al. Generación de un sustituto de mucosa oral humana y comprobación de su viabilidad mediante ingeniería tisular. Cir Pediatr 2011; 24: 13-18.19- Elias P, Matsuyoshi N, Wu H, Lin C, Wang Z, Brown B, et al. Desmoglein Isoform Distribution Affects Stratum Corneum Structure and Function. J Cell Biol. 2001; 153(2): 243-50.20- Mahoney M, Sadowski S, Brennan D, Pikander P, Saukko P, Wahl J, et al. Compound heterozygous desmoplakin mutations result in a phenotype with a combination of myocardial, skin, hair, and enamel abnormalities. J Invest Dermatol. 2010; 130(4):968-7821- Doğan A, Demirci S, Şahin F. In vitro differentiation of human tooth germ stem cells into endothelial- and epithelial-like cells. Cell Biol Int. 2015; 39(1):94-103.instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/The stem cells of the apical papilla have excellent properties of differentiation and great potential for use in tissue engineering protocols, without However, the potential for epithelial differentiation has not been explored with this cell type. The objective is to analyze the expression of cytokeratins 8, 13, and 19, desmoplaquine and desmoglein by immunofluorescence. Materials and Methods: Apical papilla tissue cell cultures were performed using Explant technique of healthy donor patients. Characterization was performed cell by flow cytometry, was analyzed by immunofluorescence the expression of cytokeratins 8, 13 and 19, desmoplakine and desmoglein. Results: The cells of the apical papilla expressed cytokeratin 8, 13 and 19 desmoplaquine and desmoglein, observed through the immunofluorescence technique, which was applied to the cells of the fourth pass that demonstrated a cell viability of 86.76% by means of cytometry of flow and trypan blue. Conclusions: The stem cells of the apical papilla express epithelial markers and support the idea that they could be used as an alternative source to develop artificial oral mucosa protocols.Las células madre de la papila apical tienen excelentes propiedades de diferenciación y gran potencial de uso en protocolos de ingeniería tisular, sin embargo, el potencial de diferenciación epitelial no ha sido explorado con este tipo de células. El objetivo es analizar la expresión de citoqueratinas 8, 13, y 19, desmoplaquina y desmogleína mediante inmunofluorescencia. Materiales y Métodos: Se realizaron cultivos celulares de tejido de papila apical mediante técnica de explante de pacientes donadores sanos. Se realizó caracterización celular mediante citometría de flujo, se analizó por medio de inmunofluorescencia la expresión de citoqueratinas 8, 13 y 19, desmoplaquina y desmogleína. Resultados: Las células de la papila apical expresaron citoqueratina 8, 13 y 19 desmoplaquina y desmogleína, observado a través de la técnica inmunofluorescencia, la cual se le aplicó a las células del cuarto pase que demostraron una viabilidad celular del 86.76% por medio de citometría de flujo y azul de tripano. Conclusiones: Las células madre de la papila apical expresan marcadores epiteliales y soportan la idea que podrían ser usadas como una fuente alternativa para desarrollar protocolos de mucosa oral artificial.Odontólogo(a)EspecializaciónPresencialspaUniversidad Antonio NariñoEspecialización en PeriodonciaFacultad de OdontologíaBogotá - Circunvalarcitoqueratinas 8, 13, 19.desmogleinadesmoplaquinapapila apicalcytokeratin 8, 13, 19desmogleindesmoplakinapical papillaIdentificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto.Trabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85ORIGINAL2020DiegoAndrésRodriguezReyes.pdf2020DiegoAndrésRodriguezReyes.pdfarticulo finalapplication/pdf485925https://repositorio.uan.edu.co/bitstreams/9854a42a-1c38-4b01-8a7b-e537f325d65c/download36f2b1dddf4d33cfa8d4b7a8f6b6ae23MD512020AutorizacióndeAutores1.pdf2020AutorizacióndeAutores1.pdfautorizacionesapplication/pdf575085https://repositorio.uan.edu.co/bitstreams/a4e30e17-93ff-4092-bd34-e4efc5ecb2f5/download846e4c4a84f7ade572bbe66e7a2c68fbMD522020AutorizacióndeAutores2.pdf2020AutorizacióndeAutores2.pdfautorizacionesapplication/pdf272392https://repositorio.uan.edu.co/bitstreams/367007fc-a869-4508-b92e-79a5f0625fe1/downloadfe15e48e090551fcbea2d32743e9bbf9MD532020AutorizacióndeAutores3.pdf2020AutorizacióndeAutores3.pdfautorizacionesapplication/pdf439095https://repositorio.uan.edu.co/bitstreams/c8c1776c-d324-4627-ae07-5e2095ec95ba/downloadb68264e0501738089a0d24591882dcafMD54LICENSElicense.txtlicense.txttext/plain; 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Identificación por inmunofluorescencia de citoqueratinas 8, 13, 19, desmoplaquina y desmogleína en células madre de la papila apical. ex vivo. Prueba piloto.

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