Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)

The insecticide chlorpyrifos is used on a variety of agricultural crops around the world. and its large-scale application has caused environmental contamination. In this work, the degradation rate of chlorpyrifos by the rhizobacterium Pseudomonas aeruginosa (TN50) from potato (Solanum tuberosum) cro...

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Autores:: Gómez Gómez, Alba Marcela

Tipo de recurso:: Trabajo de grado de pregrado

Fecha de publicación:: 2021

Institución:: Universidad Antonio Nariño

Repositorio:: Repositorio UAN

Idioma:: spa

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dc.title.es_ES.fl_str_mv	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
title	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
spellingShingle	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum) Clorpirifos 3,5,6-tricloro-2-piridinol Degradación Pseudomonas aeruginosa Genes Enzimas 540 Chlorpyrifos 3,5,6-trichloro-2-pyridinol Degradation Pseudomonas aeruginosa Genes Enzymes
title_short	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
title_full	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
title_fullStr	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
title_full_unstemmed	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
title_sort	Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)
dc.creator.fl_str_mv	Gómez Gómez, Alba Marcela
dc.contributor.advisor.spa.fl_str_mv	Martínez Pachón, Diana Vanegas Guerrero, Javier
dc.contributor.author.spa.fl_str_mv	Gómez Gómez, Alba Marcela
dc.subject.es_ES.fl_str_mv	Clorpirifos 3,5,6-tricloro-2-piridinol Degradación Pseudomonas aeruginosa Genes Enzimas
topic	Clorpirifos 3,5,6-tricloro-2-piridinol Degradación Pseudomonas aeruginosa Genes Enzimas 540 Chlorpyrifos 3,5,6-trichloro-2-pyridinol Degradation Pseudomonas aeruginosa Genes Enzymes
dc.subject.ddc.es_ES.fl_str_mv	540
dc.subject.keyword.es_ES.fl_str_mv	Chlorpyrifos 3,5,6-trichloro-2-pyridinol Degradation Pseudomonas aeruginosa Genes Enzymes
description	The insecticide chlorpyrifos is used on a variety of agricultural crops around the world. and its large-scale application has caused environmental contamination. In this work, the degradation rate of chlorpyrifos by the rhizobacterium Pseudomonas aeruginosa (TN50) from potato (Solanum tuberosum) crop in minimal salt medium was determined. Genes and enzymes were detected in the genome of TN50 that may be associated with chlorpyrifos degradation and suggest that the bacterium may degrade the insecticide as a source of phosphorus and carbon. The chromatographic and liquid-liquid extraction technique for the monitoring of chlorpyrifos and its main degradation metabolite 3,5,6 trichloro-2-pyridinol was validated and the capacity of TN50 to use the insecticide as a sole carbon source was established. A 17.43% depletion of chlorpyrifos by P. aeruginosa was determined with glucose as an external carbon source and with prior metabolic activation in medium with glucose and chlorpyrifos, indicating that TN50 does not have the capacity to use the insecticide as a sole carbon source but can degrade the compound as an external nutrient source. Additional degradation studies could determine the potential of TN50 for bioremediation of this type of pollutants.
publishDate	2021
dc.date.issued.spa.fl_str_mv	2021-11-24
dc.date.accessioned.none.fl_str_mv	2022-04-30T00:09:27Z
dc.date.available.none.fl_str_mv	2022-04-30T00:09:27Z
dc.type.spa.fl_str_mv	Trabajo de grado (Pregrado y/o Especialización)
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dc.identifier.bibliographicCitation.spa.fl_str_mv	Ministerio de Agricultura y Desarrollo Rural, Minagricultura (2020). CADENA DE LA PAPA Indicadores e instrumentos. Unknown. https://sioc.minagricultura.gov.co/Papa/Documentos/2020-06-30%20Cifras%20Sectoria les.pdf. Consultado Marzo de 2021 Federación Colombiana de Productores de Papa, FEDEPAPA (2019). Informe de Gestión 2019. Unknown. https://fedepapa.com/wp-content/uploads/2021/09/BOLETIN-ECONOMICO-N%C2% B014.pdf. Consultado Noviembre de 2021 Shabbir, M., Singh, M., Maiti, S., Kumar, S., & Saha, S. K. (2018). Removal enactment of organo-phosphorous pesticide using bacteria isolated from domestic sewage. Bioresource Technology, 263, 280–288. https://doi.org/10.1016/j.biortech.2018.04.122 Aswathi, A., Pandey, A., & Sukumaran, R. K. (2019). Rapid degradation of the organophosphate pesticide – Chlorpyrifos by a novel strain of Pseudomonas nitroreducens AR-3. Bioresource Technology, 292(August), 122025. https://doi.org/10.1016/j.biortech.2019.122025 Cámara de Comercio de Bogotá (CCB) (2015). Manual papa. Vicepresidencia de Fortalecimiento Empresarial; Programa de Apoyo Agrícola y Agroindustrial. Unknown. http://hdl.handle.net/11520/14306. Consultado Marzo de 2020 Instituto Colombiano Agropecuario, ICA (2016). ESTADÍSTICAS DE COMERCIALIZACIÓN DE PLAGUICIDAS QUÍMICOS DE USO AGRÍCOLA 2016. Unknown. https://www.ica.gov.co/areas/agricola/servicios/regulacion-y-control-de-plaguicidas-qui micos/estadisticas/cartilla-plaguicidas-2016_22-01-18.aspx. Consultado Marzo de 2020 Pohanish, R. P. (2015). C. In Sittig’s Handbook of Pesticides and Agricultural Chemicals. 2, 91–195. https://doi.org/10.1016/B978-1-4557-3148-0.00003-0 Dow AgroSciences Colombia (2012). Lorsban, insecticida agrícola, concentrado emulsionante (EC). Etiqueta Web – Colombia.. Unknown. https://www.dowagro.com/content/dam/hdas/dowagro_colombia/pdfs/0901b80380988d 82.pdf. Consultado Marzo de 2020 Singh, B. K., Walker, A., Morgan, J. A. W., & Wright, D. J. (2004). Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Applied and Environmental Microbiology, 70(8), 4855–4863. https://doi.org/10.1128/AEM.70.8.4855-4863.2004 Li, X., Jiang, J., Gu, L., Ali, S. W., He, J., & Li, S. (2008). Diversity of chlorpyrifos-degrading bacteria isolated from chlorpyrifos-contaminated samples. International Biodeterioration and Biodegradation, 62(4), 331–335. https://doi.org/10.1016/j.ibiod.2008.03.001
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identifier_str_mv	Ministerio de Agricultura y Desarrollo Rural, Minagricultura (2020). CADENA DE LA PAPA Indicadores e instrumentos. Unknown. https://sioc.minagricultura.gov.co/Papa/Documentos/2020-06-30%20Cifras%20Sectoria les.pdf. Consultado Marzo de 2021 Federación Colombiana de Productores de Papa, FEDEPAPA (2019). Informe de Gestión 2019. Unknown. https://fedepapa.com/wp-content/uploads/2021/09/BOLETIN-ECONOMICO-N%C2% B014.pdf. Consultado Noviembre de 2021 Shabbir, M., Singh, M., Maiti, S., Kumar, S., & Saha, S. K. (2018). Removal enactment of organo-phosphorous pesticide using bacteria isolated from domestic sewage. Bioresource Technology, 263, 280–288. https://doi.org/10.1016/j.biortech.2018.04.122 Aswathi, A., Pandey, A., & Sukumaran, R. K. (2019). Rapid degradation of the organophosphate pesticide – Chlorpyrifos by a novel strain of Pseudomonas nitroreducens AR-3. Bioresource Technology, 292(August), 122025. https://doi.org/10.1016/j.biortech.2019.122025 Cámara de Comercio de Bogotá (CCB) (2015). Manual papa. Vicepresidencia de Fortalecimiento Empresarial; Programa de Apoyo Agrícola y Agroindustrial. Unknown. http://hdl.handle.net/11520/14306. Consultado Marzo de 2020 Instituto Colombiano Agropecuario, ICA (2016). ESTADÍSTICAS DE COMERCIALIZACIÓN DE PLAGUICIDAS QUÍMICOS DE USO AGRÍCOLA 2016. Unknown. https://www.ica.gov.co/areas/agricola/servicios/regulacion-y-control-de-plaguicidas-qui micos/estadisticas/cartilla-plaguicidas-2016_22-01-18.aspx. Consultado Marzo de 2020 Pohanish, R. P. (2015). C. In Sittig’s Handbook of Pesticides and Agricultural Chemicals. 2, 91–195. https://doi.org/10.1016/B978-1-4557-3148-0.00003-0 Dow AgroSciences Colombia (2012). Lorsban, insecticida agrícola, concentrado emulsionante (EC). Etiqueta Web – Colombia.. Unknown. https://www.dowagro.com/content/dam/hdas/dowagro_colombia/pdfs/0901b80380988d 82.pdf. Consultado Marzo de 2020 Singh, B. K., Walker, A., Morgan, J. A. W., & Wright, D. J. (2004). Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Applied and Environmental Microbiology, 70(8), 4855–4863. https://doi.org/10.1128/AEM.70.8.4855-4863.2004 Li, X., Jiang, J., Gu, L., Ali, S. W., He, J., & Li, S. (2008). Diversity of chlorpyrifos-degrading bacteria isolated from chlorpyrifos-contaminated samples. International Biodeterioration and Biodegradation, 62(4), 331–335. https://doi.org/10.1016/j.ibiod.2008.03.001 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/
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eu_rights_str_mv	openAccess
dc.publisher.spa.fl_str_mv	Universidad Antonio Nariño
dc.publisher.program.spa.fl_str_mv	Bioquímica
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.campus.spa.fl_str_mv	Bogotá - Circunvalar
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spelling	Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Martínez Pachón, DianaVanegas Guerrero, JavierGómez Gómez, Alba Marcela118217189762022-04-30T00:09:27Z2022-04-30T00:09:27Z2021-11-24http://repositorio.uan.edu.co/handle/123456789/6376Ministerio de Agricultura y Desarrollo Rural, Minagricultura (2020). CADENA DE LA PAPA Indicadores e instrumentos. Unknown. https://sioc.minagricultura.gov.co/Papa/Documentos/2020-06-30%20Cifras%20Sectoria les.pdf. Consultado Marzo de 2021Federación Colombiana de Productores de Papa, FEDEPAPA (2019). Informe de Gestión 2019. Unknown. https://fedepapa.com/wp-content/uploads/2021/09/BOLETIN-ECONOMICO-N%C2% B014.pdf. Consultado Noviembre de 2021Shabbir, M., Singh, M., Maiti, S., Kumar, S., & Saha, S. K. (2018). Removal enactment of organo-phosphorous pesticide using bacteria isolated from domestic sewage. Bioresource Technology, 263, 280–288. https://doi.org/10.1016/j.biortech.2018.04.122Aswathi, A., Pandey, A., & Sukumaran, R. K. (2019). Rapid degradation of the organophosphate pesticide – Chlorpyrifos by a novel strain of Pseudomonas nitroreducens AR-3. Bioresource Technology, 292(August), 122025. https://doi.org/10.1016/j.biortech.2019.122025Cámara de Comercio de Bogotá (CCB) (2015). Manual papa. Vicepresidencia de Fortalecimiento Empresarial; Programa de Apoyo Agrícola y Agroindustrial. Unknown. http://hdl.handle.net/11520/14306. Consultado Marzo de 2020Instituto Colombiano Agropecuario, ICA (2016). ESTADÍSTICAS DE COMERCIALIZACIÓN DE PLAGUICIDAS QUÍMICOS DE USO AGRÍCOLA 2016. Unknown. https://www.ica.gov.co/areas/agricola/servicios/regulacion-y-control-de-plaguicidas-qui micos/estadisticas/cartilla-plaguicidas-2016_22-01-18.aspx. Consultado Marzo de 2020Pohanish, R. P. (2015). C. In Sittig’s Handbook of Pesticides and Agricultural Chemicals. 2, 91–195. https://doi.org/10.1016/B978-1-4557-3148-0.00003-0Dow AgroSciences Colombia (2012). Lorsban, insecticida agrícola, concentrado emulsionante (EC). Etiqueta Web – Colombia.. Unknown. https://www.dowagro.com/content/dam/hdas/dowagro_colombia/pdfs/0901b80380988d 82.pdf. Consultado Marzo de 2020Singh, B. K., Walker, A., Morgan, J. A. W., & Wright, D. J. (2004). Biodegradation of chlorpyrifos by Enterobacter strain B-14 and its use in bioremediation of contaminated soils. Applied and Environmental Microbiology, 70(8), 4855–4863. https://doi.org/10.1128/AEM.70.8.4855-4863.2004Li, X., Jiang, J., Gu, L., Ali, S. W., He, J., & Li, S. (2008). Diversity of chlorpyrifos-degrading bacteria isolated from chlorpyrifos-contaminated samples. International Biodeterioration and Biodegradation, 62(4), 331–335. https://doi.org/10.1016/j.ibiod.2008.03.001instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/The insecticide chlorpyrifos is used on a variety of agricultural crops around the world. and its large-scale application has caused environmental contamination. In this work, the degradation rate of chlorpyrifos by the rhizobacterium Pseudomonas aeruginosa (TN50) from potato (Solanum tuberosum) crop in minimal salt medium was determined. Genes and enzymes were detected in the genome of TN50 that may be associated with chlorpyrifos degradation and suggest that the bacterium may degrade the insecticide as a source of phosphorus and carbon. The chromatographic and liquid-liquid extraction technique for the monitoring of chlorpyrifos and its main degradation metabolite 3,5,6 trichloro-2-pyridinol was validated and the capacity of TN50 to use the insecticide as a sole carbon source was established. A 17.43% depletion of chlorpyrifos by P. aeruginosa was determined with glucose as an external carbon source and with prior metabolic activation in medium with glucose and chlorpyrifos, indicating that TN50 does not have the capacity to use the insecticide as a sole carbon source but can degrade the compound as an external nutrient source. Additional degradation studies could determine the potential of TN50 for bioremediation of this type of pollutants.El insecticida clorpirifos se usa en variedad de cultivos agrícolas de todo el mundo y su aplicación a gran escala ha provocado contaminación ambiental. En este trabajo se determina la tasa de degradación de clorpirifos por la rizobacteria Pseudomonas aeruginosa (TN50) proveniente de cultivo de papa (Solanum tuberosum) en medio mínimo de sales. Se detectaron genes y enzimas en el genoma de TN50 que pueden estar asociados a la degradación de clorpirifos y sugieren que la bacteria podría degradar el insecticida como fuente de fósforo y carbono. Se validó la técnica cromatográfica y de extracción líquido-líquido para el seguimiento de clorpirifos y su principal metabolito de degradación 3,5,6-tricloro-2-piridinol y se estableció la capacidad de TN50 para usar el insecticida como única fuente de carbono. Se determinó la disminución del 17.43% de clorpirifos por P. aeruginosa con glucosa como fuente externa de carbono y con previa activación metabólica en medio con glucosa y clorpirifos, lo que indica que TN50 no tiene la capacidad de utilizar el insecticida como única fuente de carbono pero puede llegar a degradar el compuesto como fuente externa de nutrientes. Estudios adicionales de degradación podrían determinar el potencial de TN50 para la biorremediación de este tipo de contaminantes.Bioquímico(a)PregradoPresencialInvestigaciónspaUniversidad Antonio NariñoBioquímicaFacultad de CienciasBogotá - CircunvalarClorpirifos3,5,6-tricloro-2-piridinolDegradaciónPseudomonas aeruginosaGenesEnzimas540Chlorpyrifos3,5,6-trichloro-2-pyridinolDegradationPseudomonas aeruginosaGenesEnzymesDegradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)Trabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85EspecializadaORIGINAL2021_AlbaMarcelaGómezGómez_Autorización.pdf2021_AlbaMarcelaGómezGómez_Autorización.pdfFormato de autorización de autoresapplication/pdf942824https://repositorio.uan.edu.co/bitstreams/5bbdd545-14bb-441c-9ab8-90c0c751d19c/download1ef7434f371bc4a2300a7f1316d616a1MD512021_AlbaMarcelaGómezGómez.pdf2021_AlbaMarcelaGómezGómez.pdfTrabajo de gradoapplication/pdf1764757https://repositorio.uan.edu.co/bitstreams/fe9da7cb-f83b-4b68-9ca0-30a6b3ab12a0/download3451fd6ac4c7caed36948c2a4c3276bcMD522021_AlbaMarcelaGómezGómez_Acta.pdf2021_AlbaMarcelaGómezGómez_Acta.pdfActa de sustentaciónapplication/pdf512708https://repositorio.uan.edu.co/bitstreams/fc9bf696-879e-4878-9277-ccfea7a8efcd/download959f965944ce83918b784f27f368e40fMD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.uan.edu.co/bitstreams/87931c0e-a75a-461e-b81d-f082d1f2ff14/download9868ccc48a14c8d591352b6eaf7f6239MD54CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.uan.edu.co/bitstreams/3375ab71-0752-448b-9e1c-3ed228127017/download9868ccc48a14c8d591352b6eaf7f6239MD54123456789/6376oai:repositorio.uan.edu.co:123456789/63762024-10-09 22:47:06.199https://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertorestrictedhttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co

Degradación del insecticida clorpirifos por Pseudomona aeruginosa, rizobacteria aislada de cultivo de papa (Solanum tuberosum)

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