Agrobacterium-mediated transient expression for studying cassava resistance proteins

Agrobacterium tumefaciens is a plant pathogenic bacterium that causes disease by introducing genes into plant cells. This ability of Agrobacterium has been exploited in biotechnology to introduce genes of interest and express them in plant cells. Gene expression in plant cells can be either transien...

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Autores:: Romero, Carlos
Fernández, Wendy
López, Camilo
Diaz, Paula

Tipo de recurso:: Article of investigation

Fecha de publicación:: 2020

Institución:: Universidad Antonio Nariño

Repositorio:: Repositorio UAN

Idioma:: spa

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network_acronym_str	UAntonioN2
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spelling	Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Romero, CarlosFernández, WendyLópez, CamiloDiaz, Paula2021-06-16T13:59:58Z2021-06-16T13:59:58Z2020-03-20http://revistas.uan.edu.co/index.php/saywa/article/view/690http://repositorio.uan.edu.co/handle/123456789/4453Agrobacterium tumefaciens is a plant pathogenic bacterium that causes disease by introducing genes into plant cells. This ability of Agrobacterium has been exploited in biotechnology to introduce genes of interest and express them in plant cells. Gene expression in plant cells can be either transient or stable. The implementation of transient gene expression has made it possible to understand, among many other things, the molecular strategies used by plant cells to recognize invading pathogens. For this reason, the research groups Ciencias Biológicas y Químicas at Universidad Antonio Nariño and Manihot Biotec at Universidad Nacional de Colombia have focused on expressing disease resistance proteins of cassava that confer resistance to the bacterium Xanthomonas axonopodis pv. manihotis in order to study the molecular mechanisms employed by cassava cells to recognize this bacterial pathogen.En este artículo se aborda el principio de la expresión transitoria mediada por Agrobacterium tumefaciens y su contribución en el estudio interacciones proteína-proteína en el campo de la fitopatología molecular. Adicionalmente, se expone el estado actual sobre la implementación de ensayos de expresión transitoria para el estudio de proteínas de resistencia de la yuca a la bacteriosis vascular.application/pdfspaUniversidad Antonio Nariñohttp://revistas.uan.edu.co/index.php/saywa/article/view/690/5912711-1571REVISTA SAYWA; Vol. 1 Núm. 2 (2019)Bacteriosis vascularexpresión transitoriagenes de resistenciaInmunidad vegetalXanthomonas axonopodis pv. ManihotisAgrobacterium-mediated transient expression for studying cassava resistance proteinsExpresión transitoria mediada por Agrobacterium para el estudio de proteínas de resistencia de yucaDivulgación científicainfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articlehttp://purl.org/coar/version/c_970fb48d4fbd8a85123456789/4453oai:repositorio.uan.edu.co:123456789/44532024-10-09 22:33:57.321https://creativecommons.org/licenses/by-nc-sa/4.0/Acceso abiertometadata.onlyhttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co
dc.title.en-US.fl_str_mv	Agrobacterium-mediated transient expression for studying cassava resistance proteins
dc.title.es-ES.fl_str_mv	Expresión transitoria mediada por Agrobacterium para el estudio de proteínas de resistencia de yuca
title	Agrobacterium-mediated transient expression for studying cassava resistance proteins
spellingShingle	Agrobacterium-mediated transient expression for studying cassava resistance proteins Bacteriosis vascular expresión transitoria genes de resistencia Inmunidad vegetal Xanthomonas axonopodis pv. Manihotis
title_short	Agrobacterium-mediated transient expression for studying cassava resistance proteins
title_full	Agrobacterium-mediated transient expression for studying cassava resistance proteins
title_fullStr	Agrobacterium-mediated transient expression for studying cassava resistance proteins
title_full_unstemmed	Agrobacterium-mediated transient expression for studying cassava resistance proteins
title_sort	Agrobacterium-mediated transient expression for studying cassava resistance proteins
dc.creator.fl_str_mv	Romero, Carlos Fernández, Wendy López, Camilo Diaz, Paula
dc.contributor.author.spa.fl_str_mv	Romero, Carlos Fernández, Wendy López, Camilo Diaz, Paula
dc.subject.es-ES.fl_str_mv	Bacteriosis vascular expresión transitoria genes de resistencia Inmunidad vegetal Xanthomonas axonopodis pv. Manihotis
topic	Bacteriosis vascular expresión transitoria genes de resistencia Inmunidad vegetal Xanthomonas axonopodis pv. Manihotis
description	Agrobacterium tumefaciens is a plant pathogenic bacterium that causes disease by introducing genes into plant cells. This ability of Agrobacterium has been exploited in biotechnology to introduce genes of interest and express them in plant cells. Gene expression in plant cells can be either transient or stable. The implementation of transient gene expression has made it possible to understand, among many other things, the molecular strategies used by plant cells to recognize invading pathogens. For this reason, the research groups Ciencias Biológicas y Químicas at Universidad Antonio Nariño and Manihot Biotec at Universidad Nacional de Colombia have focused on expressing disease resistance proteins of cassava that confer resistance to the bacterium Xanthomonas axonopodis pv. manihotis in order to study the molecular mechanisms employed by cassava cells to recognize this bacterial pathogen.
publishDate	2020
dc.date.issued.spa.fl_str_mv	2020-03-20
dc.date.accessioned.none.fl_str_mv	2021-06-16T13:59:58Z
dc.date.available.none.fl_str_mv	2021-06-16T13:59:58Z
dc.type.spa.fl_str_mv	Divulgación científica
dc.type.version.spa.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.coar.spa.fl_str_mv	http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.driver.spa.fl_str_mv	info:eu-repo/semantics/article
dc.type.coarversion.none.fl_str_mv	http://purl.org/coar/version/c_970fb48d4fbd8a85
format	http://purl.org/coar/resource_type/c_2df8fbb1
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://revistas.uan.edu.co/index.php/saywa/article/view/690
dc.identifier.uri.none.fl_str_mv	http://repositorio.uan.edu.co/handle/123456789/4453
url	http://revistas.uan.edu.co/index.php/saywa/article/view/690 http://repositorio.uan.edu.co/handle/123456789/4453
dc.language.iso.spa.fl_str_mv	spa
language	spa
dc.relation.none.fl_str_mv	http://revistas.uan.edu.co/index.php/saywa/article/view/690/591
dc.rights.none.fl_str_mv	Acceso abierto
dc.rights.license.spa.fl_str_mv	Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.rights.uri.spa.fl_str_mv	https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.accessrights.spa.fl_str_mv	info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv	http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv	Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) Acceso abierto https://creativecommons.org/licenses/by-nc-sa/4.0/ http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.spa.fl_str_mv	Universidad Antonio Nariño
dc.source.none.fl_str_mv	2711-1571
dc.source.es-ES.fl_str_mv	REVISTA SAYWA; Vol. 1 Núm. 2 (2019)
institution	Universidad Antonio Nariño
repository.name.fl_str_mv	Repositorio Institucional UAN
repository.mail.fl_str_mv	alertas.repositorio@uan.edu.co
_version_	1812928294531629056

Agrobacterium-mediated transient expression for studying cassava resistance proteins

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