Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación

The study of the identification of biomarkers that are linked to the seminal quality of the bull, are very important when quantifying proteins and sperm cells that determine the fertility of the bovine male. For this reason, a research internship was carried out in the Tecnho Sperm laboratory with t...

Full description

Autores:
Pacheco Martínez, Fabián Matías
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2022
Institución:
Universidad Antonio Nariño
Repositorio:
Repositorio UAN
Idioma:
spa
OAI Identifier:
oai:repositorio.uan.edu.co:123456789/6809
Acceso en línea:
http://repositorio.uan.edu.co/handle/123456789/6809
Palabra clave:
PCR en tiempo real
citometría de flujo
viabilidad
células espermáticas
real-time PCR
Flow cytometry
viability
sperm cells
Rights
openAccess
License
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
id UAntonioN2_4fd4328750c2bd85268100d3543b2790
oai_identifier_str oai:repositorio.uan.edu.co:123456789/6809
network_acronym_str UAntonioN2
network_name_str Repositorio UAN
repository_id_str
dc.title.es_ES.fl_str_mv Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación
title Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación
spellingShingle Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación
PCR en tiempo real
citometría de flujo
viabilidad
células espermáticas
real-time PCR
Flow cytometry
viability
sperm cells
title_short Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación
title_full Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación
title_fullStr Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación
title_full_unstemmed Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación
title_sort Técnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigación
dc.creator.fl_str_mv Pacheco Martínez, Fabián Matías
dc.contributor.advisor.spa.fl_str_mv Daza Bolaños, Carmen Alicia
dc.contributor.author.spa.fl_str_mv Pacheco Martínez, Fabián Matías
dc.subject.es_ES.fl_str_mv PCR en tiempo real
citometría de flujo
viabilidad
células espermáticas
topic PCR en tiempo real
citometría de flujo
viabilidad
células espermáticas
real-time PCR
Flow cytometry
viability
sperm cells
dc.subject.keyword.es_ES.fl_str_mv real-time PCR
Flow cytometry
viability
sperm cells
description The study of the identification of biomarkers that are linked to the seminal quality of the bull, are very important when quantifying proteins and sperm cells that determine the fertility of the bovine male. For this reason, a research internship was carried out in the Tecnho Sperm laboratory with the aim of acquiring training in the techniques to evaluate the seminal quality of bovine males from flow cytometry, western blot and real-time PCR, as conventional techniques. That are carried out in laboratories whose purpose is to determine the biomarkers present in the fertility of cattle. Once the techniques were applied, it was observed that they are very effective and precise to evaluate the viability of the semen from the different alterations that originate when the sperm are subjected to thermal stress during the freezing process. The hypothermic storage of bovine semen allows its cryopreservation without antibiotics, but this process is limited by the high sensitivity of sperm at the time of freezing.
publishDate 2022
dc.date.accessioned.none.fl_str_mv 2022-08-22T14:04:31Z
dc.date.available.none.fl_str_mv 2022-08-22T14:04:31Z
dc.date.issued.spa.fl_str_mv 2022-07-14
dc.type.spa.fl_str_mv Trabajo de grado (Pregrado y/o Especialización)
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
format http://purl.org/coar/resource_type/c_7a1f
dc.identifier.uri.none.fl_str_mv http://repositorio.uan.edu.co/handle/123456789/6809
dc.identifier.bibliographicCitation.spa.fl_str_mv Boe-Hansen, G. B., & Satake, N. (2019). An update on boar semen assessments by flow cytometry and CASA. Theriogenology, 137, 93–103. https://doi.org/10.1016/j.theriogenology.2019.05.043
Llavanera, M., Delgado-Bermúdez, A., Fernández-Fuertes, B., Recuero, S., Mateo, Y., Bonet, S., Barranco, I., & Yeste, M. (2019). GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm. Journal of Animal Science and Biotechnology, 10(1), 1–11. https://doi.org/10.1186/s40104-019-0370-5
Llavanera, M., Delgado-Bermúdez, A., Mateo-Otero, Y., Padilla, L., Romeu, X., Roca, J., Barranco, I., & Yeste, M. (2020). Exploring seminal plasma GSTM3 as a quality and in vivo fertility biomarker in pigs—relationship with sperm morphology. Antioxidants, 9(8), 1–14. https://doi.org/10.3390/antiox9080741
Meftahi, G. H. (2021). Applications of western blot technique : From bench to bedside. March, 1–9. https://doi.org/10.1002/bmb.21516
Memili, E., Moura, A. A., & Kaya, A. (2020). Metabolomes of sperm and seminal plasma associated with bull fertility. Animal Reproduction Science, March, 106355. https://doi.org/10.1016/j.anireprosci.2020.106355
Menezes, E. S. B., Badial, P. R., El Debaky, H., Husna, A. U., Ugur, M. R., Kaya, A., Topper, E., Bulla, C., Grant, K. E., Bolden-Tiller, O., Moura, A. A., & Memili, E. (2020). Sperm miR15a and miR-29b are associated with bull fertility. Andrologia, 52(1), 1–11. https://doi.org/10.1111/and.13412
Purdy, P. H., Graham, J. K., & Azevedo, H. C. (2021). Evaluation of boar and bull sperm capacitation and the acrosome reaction using flow cytometry. Animal Reproduction Science, 106846. https://doi.org/10.1016/j.anireprosci.2021.106846
Radtke, A., Dieckmann, K., Grobelny, F., Salzbrunn, A., & Oing, C. (2019). Expression of miRNA-371a-3p in seminal plasma and ejaculate is associated with sperm concentration. 469–474. https://doi.org/10.1111/andr.12664
Salas-Huetos, A., Blanco, J., Vidal, F., Godo, A., Grossmann, M., Pons, M. C., F-Fernández, S., Garrido, N., & Anton, E. (2015). Spermatozoa from patients with seminal alterations exhibit a differential micro-ribonucleic acid profile. Fertility and Sterility, 104(3), 591–601. https://doi.org/10.1016/j.fertnstert.2015.06.015
dc.identifier.instname.spa.fl_str_mv instname:Universidad Antonio Nariño
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional UAN
dc.identifier.repourl.spa.fl_str_mv repourl:https://repositorio.uan.edu.co/
url http://repositorio.uan.edu.co/handle/123456789/6809
identifier_str_mv Boe-Hansen, G. B., & Satake, N. (2019). An update on boar semen assessments by flow cytometry and CASA. Theriogenology, 137, 93–103. https://doi.org/10.1016/j.theriogenology.2019.05.043
Llavanera, M., Delgado-Bermúdez, A., Fernández-Fuertes, B., Recuero, S., Mateo, Y., Bonet, S., Barranco, I., & Yeste, M. (2019). GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm. Journal of Animal Science and Biotechnology, 10(1), 1–11. https://doi.org/10.1186/s40104-019-0370-5
Llavanera, M., Delgado-Bermúdez, A., Mateo-Otero, Y., Padilla, L., Romeu, X., Roca, J., Barranco, I., & Yeste, M. (2020). Exploring seminal plasma GSTM3 as a quality and in vivo fertility biomarker in pigs—relationship with sperm morphology. Antioxidants, 9(8), 1–14. https://doi.org/10.3390/antiox9080741
Meftahi, G. H. (2021). Applications of western blot technique : From bench to bedside. March, 1–9. https://doi.org/10.1002/bmb.21516
Memili, E., Moura, A. A., & Kaya, A. (2020). Metabolomes of sperm and seminal plasma associated with bull fertility. Animal Reproduction Science, March, 106355. https://doi.org/10.1016/j.anireprosci.2020.106355
Menezes, E. S. B., Badial, P. R., El Debaky, H., Husna, A. U., Ugur, M. R., Kaya, A., Topper, E., Bulla, C., Grant, K. E., Bolden-Tiller, O., Moura, A. A., & Memili, E. (2020). Sperm miR15a and miR-29b are associated with bull fertility. Andrologia, 52(1), 1–11. https://doi.org/10.1111/and.13412
Purdy, P. H., Graham, J. K., & Azevedo, H. C. (2021). Evaluation of boar and bull sperm capacitation and the acrosome reaction using flow cytometry. Animal Reproduction Science, 106846. https://doi.org/10.1016/j.anireprosci.2021.106846
Radtke, A., Dieckmann, K., Grobelny, F., Salzbrunn, A., & Oing, C. (2019). Expression of miRNA-371a-3p in seminal plasma and ejaculate is associated with sperm concentration. 469–474. https://doi.org/10.1111/andr.12664
Salas-Huetos, A., Blanco, J., Vidal, F., Godo, A., Grossmann, M., Pons, M. C., F-Fernández, S., Garrido, N., & Anton, E. (2015). Spermatozoa from patients with seminal alterations exhibit a differential micro-ribonucleic acid profile. Fertility and Sterility, 104(3), 591–601. https://doi.org/10.1016/j.fertnstert.2015.06.015
instname:Universidad Antonio Nariño
reponame:Repositorio Institucional UAN
repourl:https://repositorio.uan.edu.co/
dc.language.iso.spa.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv Acceso abierto
dc.rights.license.spa.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.uri.spa.fl_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.accessrights.spa.fl_str_mv info:eu-repo/semantics/openAccess
dc.rights.coar.spa.fl_str_mv http://purl.org/coar/access_right/c_abf2
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Acceso abierto
https://creativecommons.org/licenses/by-nc-nd/4.0/
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.coverage.spatial.spa.fl_str_mv Colombia(Popayán-cauca)
dc.publisher.spa.fl_str_mv Universidad Antonio Nariño
dc.publisher.program.spa.fl_str_mv Medicina Veterinaria
dc.publisher.faculty.spa.fl_str_mv Facultad de Medicina Veterinaria
dc.publisher.campus.spa.fl_str_mv Popayán - Alto Cauca
institution Universidad Antonio Nariño
bitstream.url.fl_str_mv https://repositorio.uan.edu.co/bitstreams/42e05c22-5a8a-4153-9bb2-3a75f3569346/download
https://repositorio.uan.edu.co/bitstreams/baf21e3c-3c10-4a7c-a4bd-7e6b76a35bf2/download
https://repositorio.uan.edu.co/bitstreams/057465c4-9b4b-4a29-b881-8be928e24ce5/download
https://repositorio.uan.edu.co/bitstreams/69714320-676b-4237-bb3a-f3f98201ff75/download
bitstream.checksum.fl_str_mv 52f3204339ccf827b3c43e8dbe1c0741
0512e8af7f97e1324630ffe6e5dfc6a6
208640b2ea3297a14ff4bfd49c9691d1
9868ccc48a14c8d591352b6eaf7f6239
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
MD5
repository.name.fl_str_mv Repositorio Institucional UAN
repository.mail.fl_str_mv alertas.repositorio@uan.edu.co
_version_ 1814300292887347200
spelling Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Daza Bolaños, Carmen AliciaPacheco Martínez, Fabián Matías93339074Colombia(Popayán-cauca)2022-08-22T14:04:31Z2022-08-22T14:04:31Z2022-07-14http://repositorio.uan.edu.co/handle/123456789/6809Boe-Hansen, G. B., & Satake, N. (2019). An update on boar semen assessments by flow cytometry and CASA. Theriogenology, 137, 93–103. https://doi.org/10.1016/j.theriogenology.2019.05.043Llavanera, M., Delgado-Bermúdez, A., Fernández-Fuertes, B., Recuero, S., Mateo, Y., Bonet, S., Barranco, I., & Yeste, M. (2019). GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm. Journal of Animal Science and Biotechnology, 10(1), 1–11. https://doi.org/10.1186/s40104-019-0370-5Llavanera, M., Delgado-Bermúdez, A., Mateo-Otero, Y., Padilla, L., Romeu, X., Roca, J., Barranco, I., & Yeste, M. (2020). Exploring seminal plasma GSTM3 as a quality and in vivo fertility biomarker in pigs—relationship with sperm morphology. Antioxidants, 9(8), 1–14. https://doi.org/10.3390/antiox9080741Meftahi, G. H. (2021). Applications of western blot technique : From bench to bedside. March, 1–9. https://doi.org/10.1002/bmb.21516Memili, E., Moura, A. A., & Kaya, A. (2020). Metabolomes of sperm and seminal plasma associated with bull fertility. Animal Reproduction Science, March, 106355. https://doi.org/10.1016/j.anireprosci.2020.106355Menezes, E. S. B., Badial, P. R., El Debaky, H., Husna, A. U., Ugur, M. R., Kaya, A., Topper, E., Bulla, C., Grant, K. E., Bolden-Tiller, O., Moura, A. A., & Memili, E. (2020). Sperm miR15a and miR-29b are associated with bull fertility. Andrologia, 52(1), 1–11. https://doi.org/10.1111/and.13412Purdy, P. H., Graham, J. K., & Azevedo, H. C. (2021). Evaluation of boar and bull sperm capacitation and the acrosome reaction using flow cytometry. Animal Reproduction Science, 106846. https://doi.org/10.1016/j.anireprosci.2021.106846Radtke, A., Dieckmann, K., Grobelny, F., Salzbrunn, A., & Oing, C. (2019). Expression of miRNA-371a-3p in seminal plasma and ejaculate is associated with sperm concentration. 469–474. https://doi.org/10.1111/andr.12664Salas-Huetos, A., Blanco, J., Vidal, F., Godo, A., Grossmann, M., Pons, M. C., F-Fernández, S., Garrido, N., & Anton, E. (2015). Spermatozoa from patients with seminal alterations exhibit a differential micro-ribonucleic acid profile. Fertility and Sterility, 104(3), 591–601. https://doi.org/10.1016/j.fertnstert.2015.06.015instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/The study of the identification of biomarkers that are linked to the seminal quality of the bull, are very important when quantifying proteins and sperm cells that determine the fertility of the bovine male. For this reason, a research internship was carried out in the Tecnho Sperm laboratory with the aim of acquiring training in the techniques to evaluate the seminal quality of bovine males from flow cytometry, western blot and real-time PCR, as conventional techniques. That are carried out in laboratories whose purpose is to determine the biomarkers present in the fertility of cattle. Once the techniques were applied, it was observed that they are very effective and precise to evaluate the viability of the semen from the different alterations that originate when the sperm are subjected to thermal stress during the freezing process. The hypothermic storage of bovine semen allows its cryopreservation without antibiotics, but this process is limited by the high sensitivity of sperm at the time of freezing.El estudio de la identificación de los biomarcadores que estén vinculados con la calidad seminal del toro, son muy importantes al momento de cuantificar proteínas y células espermáticas que determinen la fertilidad del macho bovino. Por tal motivo se realizó una pasantía investigativa en el laboratorio Tecnho Sperm con el ánimo de adquirir un entrenamiento en las técnicas para evaluar la calidad seminal del macho bovino a partir de la citometría de flujo, western blot y PCR en tiempo real, como técnicas convencionales que se realizan en los laboratorios que tienen como fin determinar los biomarcadores presentes en la fertilidad del ganado bovino. Una vez aplicadas las técnicas se pudo observar que son muy eficaces y precisas para evaluar la viabilidad del semen a partir de las diferentes alteraciones que se originan al momento de someter los espermatozoides al estrés térmico durante el proceso congelación. El almacenamiento hipotérmico del semen del bovino permite su criopreservación sin antibióticos, pero este proceso está limitado a la alta sensibilidad que tiene los espermatozoides al momento de su congelaciónMédico(a) Veterinario(a)PregradoPresencialPasantíaspaUniversidad Antonio NariñoMedicina VeterinariaFacultad de Medicina VeterinariaPopayán - Alto CaucaPCR en tiempo realcitometría de flujoviabilidadcélulas espermáticasreal-time PCRFlow cytometryviabilitysperm cellsTécnicas moleculares para determinar la calidad seminal del bovino sometido a criotolerancia: informe de pasantía de investigaciónTrabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85GeneralORIGINAL2022_FabiánPacheco2022_FabiánPachecoTrabajo de gradoapplication/pdf397590https://repositorio.uan.edu.co/bitstreams/42e05c22-5a8a-4153-9bb2-3a75f3569346/download52f3204339ccf827b3c43e8dbe1c0741MD522022_FabiánPacheco_Acta2022_FabiánPacheco_ActaActa sustentaciónapplication/pdf181169https://repositorio.uan.edu.co/bitstreams/baf21e3c-3c10-4a7c-a4bd-7e6b76a35bf2/download0512e8af7f97e1324630ffe6e5dfc6a6MD512022_FabiánPacheco_Autorización2022_FabiánPacheco_AutorizaciónAutorización bibliotecaapplication/pdf1187589https://repositorio.uan.edu.co/bitstreams/057465c4-9b4b-4a29-b881-8be928e24ce5/download208640b2ea3297a14ff4bfd49c9691d1MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.uan.edu.co/bitstreams/69714320-676b-4237-bb3a-f3f98201ff75/download9868ccc48a14c8d591352b6eaf7f6239MD54123456789/6809oai:repositorio.uan.edu.co:123456789/68092024-10-09 22:35:38.555https://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertoopen.accesshttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co

Publicaciones similares