Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma

Glioblastoma is the most aggressive and malignant tumor of the brain, with a survival rate of no more than 12 months. Currently, resistance to treatments has been evidenced, so there is a need to explore adjuvant therapies to those that already exist. Among them is the ketogenic diet based on medium...

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Autores:: Garcia Daza, Dayana Katerine

Tipo de recurso:: Trabajo de grado de pregrado

Fecha de publicación:: 2022

Institución:: Universidad Antonio Nariño

Repositorio:: Repositorio UAN

Idioma:: spa

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dc.title.es_ES.fl_str_mv	Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma
title	Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma
spellingShingle	Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma glioblastoma decanoic acid octanoic acid oxidative stress proliferation 572 glioblastoma decanoic acid octanoic acid oxidative stress proliferation
title_short	Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma
title_full	Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma
title_fullStr	Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma
title_full_unstemmed	Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma
title_sort	Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma
dc.creator.fl_str_mv	Garcia Daza, Dayana Katerine
dc.contributor.advisor.spa.fl_str_mv	Losada Barragán, Mónica Upegui Zapata, Yulieth Alexandra
dc.contributor.author.spa.fl_str_mv	Garcia Daza, Dayana Katerine
dc.subject.es_ES.fl_str_mv	glioblastoma decanoic acid octanoic acid oxidative stress proliferation
topic	glioblastoma decanoic acid octanoic acid oxidative stress proliferation 572 glioblastoma decanoic acid octanoic acid oxidative stress proliferation
dc.subject.ddc.es_ES.fl_str_mv	572
dc.subject.keyword.es_ES.fl_str_mv	glioblastoma decanoic acid octanoic acid oxidative stress proliferation
description	Glioblastoma is the most aggressive and malignant tumor of the brain, with a survival rate of no more than 12 months. Currently, resistance to treatments has been evidenced, so there is a need to explore adjuvant therapies to those that already exist. Among them is the ketogenic diet based on medium-chain free fatty acids, which has shown antiangiogenic, anti-inflammatory and proapoptotic properties in various diseases. In this study, cell overload, oxidative stress, and the relative expression of PTEN, CCND3, HIF 1α, and BCL2 tumor genes in T98G cells after octanoic and decanoic acid supplementation for 3 days were evaluated. A reduction in cell turnover, superoxide levels and reactive oxygen species was found in both octanoic acid and decanoic acid treated cells. In addition, a reduction of BCL2 in cells with decanoic acid and an increase in the expression of PTEN with low glucose octanoic acid is evidenced, which suggests a recovery in the control of cell death. The study demonstrates the potential in vitro use of medium chain fatty acids as adjuvant therapy
publishDate	2022
dc.date.issued.spa.fl_str_mv	2022-12-13
dc.date.accessioned.none.fl_str_mv	2023-01-17T17:23:55Z
dc.date.available.none.fl_str_mv	2023-01-17T17:23:55Z
dc.type.spa.fl_str_mv	Trabajo de grado (Pregrado y/o Especialización)
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dc.identifier.uri.none.fl_str_mv	http://repositorio.uan.edu.co/handle/123456789/7386
dc.identifier.bibliographicCitation.spa.fl_str_mv	abcam. (2021, December 29). ROS/Superoxide Detection Assay Kit (Cell-based) (ab139476). Alves, A. L. v, Gomes, I. N. F., Carloni, A. C., Rosa, M. N., da Silva, L. S., Evangelista, A. F., Reis, R. M., & Silva, V. A. O. (2021). Role of glioblastoma stem cells in cancer therapeutic resistance: a perspective on antineoplastic agents from natural sources and chemical derivatives. Stem Cell Research & Therapy, 12(1), 206. https://doi.org/10.1186/s13287-021-02231-x Andersen, J., Westi, E., Jakobsen, E., Urruticoechea, N., Borges, K., & Aldana, B. (2021). Astrocyte metabolism of the medium-chain fatty acids octanoic acid and decanoic acid promotes GABA synthesis in neurons via elevated glutamine supply. Molecular Brain, 14(1), 132. Augustin, K., Khabbush, A., Williams, S., Eaton, S., Orford, M., Cross, J. H., Heales, S. J. R., Walker, M. C., & Williams, R. S. B. (2018). Mechanisms of action for the medium-chain triglyceride ketogenic diet in neurological and metabolic disorders. The Lancet Neurology, 17(1), 84–93. https://doi.org/10.1016/S1474-4422(17)30408-8 Avgerinos, K. I., Egan, J. M., Mattson, M. P., & Kapogiannis, D. (2020). Medium Chain Triglycerides induce mild ketosis and may improve cognition in Alzheimer’s disease. A systematic review and meta-analysis of human studies. Ageing Research Reviews, 58, 101001. https://doi.org/10.1016/j.arr.2019.101001 Brose, S. A., Marquardt, A. L., & Golovko, M. Y. (2014). Fatty acid biosynthesis from glutamate and glutamine is specifically induced in neuronal cells under hypoxia. Journal of Neurochemistry, 129(3), 400–412. https://doi.org/https://doi.org/10.1111/jnc.12617 Büschges, R., Weber, R. G., Actor, B., Lichter, P., Collins, V. P., & Reifenberger, G. (1999). Amplification and Expression of Cyclin D Genes (CCND1 CCND2 and CCND3) in Human Malignant Gliomas. Brain Pathology, 9(3), 435–442. https://doi.org/https://doi.org/10.1111/j.1750-3639.1999.tb00532.x Chang, H. T., Olson, L. K., & Schwartz, K. A. (2013). Ketolytic and glycolytic enzymatic expression profiles in malignant gliomas: implication for ketogenic diet therapy. Nutrition & Metabolism, 10(1), 47. https://doi.org/10.1186/1743-7075-10-47 Chinot, O. L., Wick, W., Mason, W., Henriksson, R., Saran, F., Nishikawa, R., Carpentier, A. F., HoangXuan, K., Kavan, P., Cernea, D., Brandes, A. A., Hilton, M., Abrey, L., & Cloughesy, T. (2014). Bevacizumab plus Radiotherapy–Temozolomide for Newly Diagnosed Glioblastoma. New England Journal of Medicine, 370(8), 709–722. https://doi.org/10.1056/NEJMoa1308345 Cunnane, S. C., Trushina, E., Morland, C., Prigione, A., Casadesus, G., Andrews, Z. B., Beal, M. F., Bergersen, L. H., Brinton, R. D., de la Monte, S., Eckert, A., Harvey, J., Jeggo, R., Jhamandas, J. H., Kann, O., la Cour, C. M., Martin, W. F., Mithieux, G., Moreira, P. I., … Millan, M. J. (2020). Brain energy rescue: an emerging therapeutic concept for neurodegenerative disorders of ageing. Nature Reviews. Drug Discovery, 19(9), 609–633. https://doi.org/10.1038/s41573-020-0072-x
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identifier_str_mv	abcam. (2021, December 29). ROS/Superoxide Detection Assay Kit (Cell-based) (ab139476). Alves, A. L. v, Gomes, I. N. F., Carloni, A. C., Rosa, M. N., da Silva, L. S., Evangelista, A. F., Reis, R. M., & Silva, V. A. O. (2021). Role of glioblastoma stem cells in cancer therapeutic resistance: a perspective on antineoplastic agents from natural sources and chemical derivatives. Stem Cell Research & Therapy, 12(1), 206. https://doi.org/10.1186/s13287-021-02231-x Andersen, J., Westi, E., Jakobsen, E., Urruticoechea, N., Borges, K., & Aldana, B. (2021). Astrocyte metabolism of the medium-chain fatty acids octanoic acid and decanoic acid promotes GABA synthesis in neurons via elevated glutamine supply. Molecular Brain, 14(1), 132. Augustin, K., Khabbush, A., Williams, S., Eaton, S., Orford, M., Cross, J. H., Heales, S. J. R., Walker, M. C., & Williams, R. S. B. (2018). Mechanisms of action for the medium-chain triglyceride ketogenic diet in neurological and metabolic disorders. The Lancet Neurology, 17(1), 84–93. https://doi.org/10.1016/S1474-4422(17)30408-8 Avgerinos, K. I., Egan, J. M., Mattson, M. P., & Kapogiannis, D. (2020). Medium Chain Triglycerides induce mild ketosis and may improve cognition in Alzheimer’s disease. A systematic review and meta-analysis of human studies. Ageing Research Reviews, 58, 101001. https://doi.org/10.1016/j.arr.2019.101001 Brose, S. A., Marquardt, A. L., & Golovko, M. Y. (2014). Fatty acid biosynthesis from glutamate and glutamine is specifically induced in neuronal cells under hypoxia. Journal of Neurochemistry, 129(3), 400–412. https://doi.org/https://doi.org/10.1111/jnc.12617 Büschges, R., Weber, R. G., Actor, B., Lichter, P., Collins, V. P., & Reifenberger, G. (1999). Amplification and Expression of Cyclin D Genes (CCND1 CCND2 and CCND3) in Human Malignant Gliomas. Brain Pathology, 9(3), 435–442. https://doi.org/https://doi.org/10.1111/j.1750-3639.1999.tb00532.x Chang, H. T., Olson, L. K., & Schwartz, K. A. (2013). Ketolytic and glycolytic enzymatic expression profiles in malignant gliomas: implication for ketogenic diet therapy. Nutrition & Metabolism, 10(1), 47. https://doi.org/10.1186/1743-7075-10-47 Chinot, O. L., Wick, W., Mason, W., Henriksson, R., Saran, F., Nishikawa, R., Carpentier, A. F., HoangXuan, K., Kavan, P., Cernea, D., Brandes, A. A., Hilton, M., Abrey, L., & Cloughesy, T. (2014). Bevacizumab plus Radiotherapy–Temozolomide for Newly Diagnosed Glioblastoma. New England Journal of Medicine, 370(8), 709–722. https://doi.org/10.1056/NEJMoa1308345 Cunnane, S. C., Trushina, E., Morland, C., Prigione, A., Casadesus, G., Andrews, Z. B., Beal, M. F., Bergersen, L. H., Brinton, R. D., de la Monte, S., Eckert, A., Harvey, J., Jeggo, R., Jhamandas, J. H., Kann, O., la Cour, C. M., Martin, W. F., Mithieux, G., Moreira, P. I., … Millan, M. J. (2020). Brain energy rescue: an emerging therapeutic concept for neurodegenerative disorders of ageing. Nature Reviews. Drug Discovery, 19(9), 609–633. https://doi.org/10.1038/s41573-020-0072-x instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/
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dc.rights.none.fl_str_mv	Acceso abierto
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dc.publisher.spa.fl_str_mv	Universidad Antonio Nariño
dc.publisher.program.spa.fl_str_mv	Bioquímica
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.campus.spa.fl_str_mv	Bogotá - Circunvalar
institution	Universidad Antonio Nariño
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spelling	Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Losada Barragán, MónicaUpegui Zapata, Yulieth AlexandraGarcia Daza, Dayana Katerine118218278652023-01-17T17:23:55Z2023-01-17T17:23:55Z2022-12-13http://repositorio.uan.edu.co/handle/123456789/7386abcam. (2021, December 29). ROS/Superoxide Detection Assay Kit (Cell-based) (ab139476).Alves, A. L. v, Gomes, I. N. F., Carloni, A. C., Rosa, M. N., da Silva, L. S., Evangelista, A. F., Reis, R. M., & Silva, V. A. O. (2021). Role of glioblastoma stem cells in cancer therapeutic resistance: a perspective on antineoplastic agents from natural sources and chemical derivatives. Stem Cell Research & Therapy, 12(1), 206. https://doi.org/10.1186/s13287-021-02231-xAndersen, J., Westi, E., Jakobsen, E., Urruticoechea, N., Borges, K., & Aldana, B. (2021). Astrocyte metabolism of the medium-chain fatty acids octanoic acid and decanoic acid promotes GABA synthesis in neurons via elevated glutamine supply. Molecular Brain, 14(1), 132.Augustin, K., Khabbush, A., Williams, S., Eaton, S., Orford, M., Cross, J. H., Heales, S. J. R., Walker, M. C., & Williams, R. S. B. (2018). Mechanisms of action for the medium-chain triglyceride ketogenic diet in neurological and metabolic disorders. The Lancet Neurology, 17(1), 84–93. https://doi.org/10.1016/S1474-4422(17)30408-8Avgerinos, K. I., Egan, J. M., Mattson, M. P., & Kapogiannis, D. (2020). Medium Chain Triglycerides induce mild ketosis and may improve cognition in Alzheimer’s disease. A systematic review and meta-analysis of human studies. Ageing Research Reviews, 58, 101001. https://doi.org/10.1016/j.arr.2019.101001Brose, S. A., Marquardt, A. L., & Golovko, M. Y. (2014). Fatty acid biosynthesis from glutamate and glutamine is specifically induced in neuronal cells under hypoxia. Journal of Neurochemistry, 129(3), 400–412. https://doi.org/https://doi.org/10.1111/jnc.12617Büschges, R., Weber, R. G., Actor, B., Lichter, P., Collins, V. P., & Reifenberger, G. (1999). Amplification and Expression of Cyclin D Genes (CCND1 CCND2 and CCND3) in Human Malignant Gliomas. Brain Pathology, 9(3), 435–442. https://doi.org/https://doi.org/10.1111/j.1750-3639.1999.tb00532.xChang, H. T., Olson, L. K., & Schwartz, K. A. (2013). Ketolytic and glycolytic enzymatic expression profiles in malignant gliomas: implication for ketogenic diet therapy. Nutrition & Metabolism, 10(1), 47. https://doi.org/10.1186/1743-7075-10-47Chinot, O. L., Wick, W., Mason, W., Henriksson, R., Saran, F., Nishikawa, R., Carpentier, A. F., HoangXuan, K., Kavan, P., Cernea, D., Brandes, A. A., Hilton, M., Abrey, L., & Cloughesy, T. (2014). Bevacizumab plus Radiotherapy–Temozolomide for Newly Diagnosed Glioblastoma. New England Journal of Medicine, 370(8), 709–722. https://doi.org/10.1056/NEJMoa1308345Cunnane, S. C., Trushina, E., Morland, C., Prigione, A., Casadesus, G., Andrews, Z. B., Beal, M. F., Bergersen, L. H., Brinton, R. D., de la Monte, S., Eckert, A., Harvey, J., Jeggo, R., Jhamandas, J. H., Kann, O., la Cour, C. M., Martin, W. F., Mithieux, G., Moreira, P. I., … Millan, M. J. (2020). Brain energy rescue: an emerging therapeutic concept for neurodegenerative disorders of ageing. Nature Reviews. Drug Discovery, 19(9), 609–633. https://doi.org/10.1038/s41573-020-0072-xinstname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/Glioblastoma is the most aggressive and malignant tumor of the brain, with a survival rate of no more than 12 months. Currently, resistance to treatments has been evidenced, so there is a need to explore adjuvant therapies to those that already exist. Among them is the ketogenic diet based on medium-chain free fatty acids, which has shown antiangiogenic, anti-inflammatory and proapoptotic properties in various diseases. In this study, cell overload, oxidative stress, and the relative expression of PTEN, CCND3, HIF 1α, and BCL2 tumor genes in T98G cells after octanoic and decanoic acid supplementation for 3 days were evaluated. A reduction in cell turnover, superoxide levels and reactive oxygen species was found in both octanoic acid and decanoic acid treated cells. In addition, a reduction of BCL2 in cells with decanoic acid and an increase in the expression of PTEN with low glucose octanoic acid is evidenced, which suggests a recovery in the control of cell death. The study demonstrates the potential in vitro use of medium chain fatty acids as adjuvant therapyEl glioblastoma es el tumor más agresivo y maligno del cerebro, con una tasa de supervivencia no mayor a los 12 meses. En la actualidad se ha evidenciado resistencia a los tratamientos, por lo que hay una necesidad de explorar terapias adyuvantes de los ya existentes. Entre los que se encuentra la dieta cetogénica a base de ácidos grasos libres de cadena media que ha demostrado propiedades antiangiogénicas, antiinflamatorias y proapoptóticas en diversas enfermedades. En este estudio se evaluó la proliferación celular, el estrés oxidativo, y la expresión relativa de los genes tumorales PTEN, CCND3, HIF 1α y BCL2 en las células T98G después de la adición de ácido octanoico y decanoico durante 3 días. Se encontró una reducción en la proliferación celular, en los niveles de superóxido y especies reactivas de oxígeno tanto en células tratadas con ácido octanoico como con ácido decanoico. Además, se evidenció una reducción de BCL2 en células con ácido decanoico y un aumento en la expresión de PTEN con ácido octanoico bajo en glucosa lo que sugiere una recuperación en el control de muerte celular. El estudio demuestra el uso potencial in vitro de los ácidos grasos de cadena media como terapia adyuvanteBioquímico(a)PregradoPresencialInvestigaciónspaUniversidad Antonio NariñoBioquímicaFacultad de CienciasBogotá - Circunvalarglioblastomadecanoic acidoctanoic acidoxidative stressproliferation572glioblastomadecanoic acidoctanoic acidoxidative stressproliferationEvaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastomaTrabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85EspecializadaORIGINAL2023_ DayanaKaterineGarciaDaza_Autorizacion.pdf2023_ DayanaKaterineGarciaDaza_Autorizacion.pdfapplication/pdf826728https://repositorio.uan.edu.co/bitstreams/548ec3a1-237b-44f0-aab8-21b3151e470a/downloadc7d4d5976e3024088e20a91f1b688e8eMD512023_ DayanaKaterineGarciaDaza_Acta.pdf2023_ DayanaKaterineGarciaDaza_Acta.pdfapplication/pdf112559https://repositorio.uan.edu.co/bitstreams/80420d0e-b6d9-4d70-92c9-6f4d8d37cd1b/download5c5a23e8d72d44b08fb167ab6bb674f9MD522023_DayanaKaterineGarciaDaza.pdf2023_DayanaKaterineGarciaDaza.pdfapplication/pdf568546https://repositorio.uan.edu.co/bitstreams/d6af53d9-5e48-4cec-970f-0e596de29f4f/download70d973878fa066f33e630ec98c0f24d0MD53123456789/7386oai:repositorio.uan.edu.co:123456789/73862024-10-09 23:07:16.373https://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertoopen.accesshttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co

Evaluación in vitro de los ácidos octanoico y decanoico como tratamiento para el cáncer de glioblastoma

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