Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis

Xanthomonas phaseoli pv. manihotis (Xpm) is the causal agent of cassava bacterial blight, a disease that can generate considerable losses in this crop. This bacterium possesses effector proteins that are injected into the host cell through the type-three secretion system and that function as virulen...

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Autores:
Santamaría Rodríguez, Brian David
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2020
Institución:
Universidad Antonio Nariño
Repositorio:
Repositorio UAN
Idioma:
spa
OAI Identifier:
oai:repositorio.uan.edu.co:123456789/1600
Acceso en línea:
http://repositorio.uan.edu.co/handle/123456789/1600
Palabra clave:
bacteriosis vascular de la yuca, promotor trampa, respuesta hipersensible.
cassava bacterial blight, trap promoter, hypersensitive response.
Rights
openAccess
License
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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oai_identifier_str oai:repositorio.uan.edu.co:123456789/1600
network_acronym_str UAntonioN2
network_name_str Repositorio UAN
repository_id_str
dc.title.es_ES.fl_str_mv Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis
title Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis
spellingShingle Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis
bacteriosis vascular de la yuca, promotor trampa, respuesta hipersensible.
cassava bacterial blight, trap promoter, hypersensitive response.
title_short Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis
title_full Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis
title_fullStr Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis
title_full_unstemmed Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis
title_sort Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotis
dc.creator.fl_str_mv Santamaría Rodríguez, Brian David
dc.contributor.advisor.spa.fl_str_mv Díaz Tatis, Paula Alejandra
Sanchez Ferro, Juan Sebastian
dc.contributor.author.spa.fl_str_mv Santamaría Rodríguez, Brian David
dc.subject.es_ES.fl_str_mv bacteriosis vascular de la yuca, promotor trampa, respuesta hipersensible.
topic bacteriosis vascular de la yuca, promotor trampa, respuesta hipersensible.
cassava bacterial blight, trap promoter, hypersensitive response.
dc.subject.keyword.es_ES.fl_str_mv cassava bacterial blight, trap promoter, hypersensitive response.
description Xanthomonas phaseoli pv. manihotis (Xpm) is the causal agent of cassava bacterial blight, a disease that can generate considerable losses in this crop. This bacterium possesses effector proteins that are injected into the host cell through the type-three secretion system and that function as virulence factors. Some of these effectors, called TALEs (Transcription-Activator Like Effectors), enter the nucleus of host cells, bind specifically to host DNA sequences, called effector-binding elements (EBEs), and act as transcription factors to modulate host gene expression. The knowledge of the mode of action for TALEs in species as rice has allowed the development of biotechnological strategies to generate broad-spectrum resistance to pathogens. In this study, a trap promoter was developed that is activated by three predominant TALEs in Xpm strains that allow the expression of the resistance gene RXam2(MHV). Our results show that when TALEs 14, 20 and 22 are co-infiltrated with the synthetic promoter that contains the EBEs for this TALEs a hypersensitive response is produced in the infiltrated area in leaves of N. tabacum. These results represent an important achivement for the use of this strategy in future cassava breeding programs.
publishDate 2020
dc.date.issued.spa.fl_str_mv 2020-06-03
dc.date.accessioned.none.fl_str_mv 2021-02-22T14:28:01Z
dc.date.available.none.fl_str_mv 2021-02-22T14:28:01Z
dc.type.spa.fl_str_mv Trabajo de grado (Pregrado y/o Especialización)
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format http://purl.org/coar/resource_type/c_7a1f
dc.identifier.uri.none.fl_str_mv http://repositorio.uan.edu.co/handle/123456789/1600
dc.identifier.bibliographicCitation.spa.fl_str_mv Aguilera, M. (2012). a Agroindustrial. Centro de Estudios Económicos Regionales (CEER) - Cartagena, 158. Retrieved from http://www.banrep.gov.co/docum/Lectura_finanzas/pdf/dtser_158.pdf
Boch, J., Bonas, U., & Lahaye, T. (2014). TAL effectors - pathogen strategies and plant resistance engineering. New Phytologist, 204(4), 823–832. https://doi.org/10.1111/nph.13015
Cohn, M., Bart, R. S., Shybut, M., Dahlbeck, D., Gomez, M., Morbitzer, R., … Staskawicz, B. J. (2014). Xanthomonas axonopodis virulence is promoted by a transcription activator-like effector - Mediated induction of a SWEET sugar transporter in Cassava. Molecular Plant-Microbe Interactions, 27(11), 1–13. https://doi.org/10.1094/MPMI-06-14-0161-R
Díaz, P. (2016). Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight ( CBB ). 1–187.
FAO. (2008). Yuca para la seguridad alimentaria y energética. Sala De Prensa, 3–4. Retrieved from http://www.fao.org/newsroom/es/news/2008/1000899/index.html
FAO. (2013). Noticias: La yuca tiene gran potencial como cultivo del siglo XXI. 28/05/2013, 3–5. Retrieved from http://www.fao.org/news/story/es/item/176821/icode/
FAO. (2017). FAOSTAT Database. Crops. Retrieved August 26, 2019, from http://www.fao.org/faostat/en/#data/QC
Grau, J., Wolf, A., Reschke, M., Bonas, U., Posch, S., & Boch, J. (2013). Computational Predictions Provide Insights into the Biology of TAL Effector Target Sites. PLoS Computational Biology, 9(3), 1–20. https://doi.org/10.1371/journal.pcbi.1002962
Hummel, A. W., Doyle, E. L., & Bogdanove, A. J. (2012). Addition of transcription activator-like effector binding sites to a pathogen strain-specific rice bacterial blight resistance gene makes it effective against additional strains and against bacterial leaf streak. New Phytologist, 195(4), 883–893. https://doi.org/10.1111/j.1469-8137.2012.04216.x
Jacques, M.-A., Arlat, M., Boulanger, A., Boureau, T., Carrère, S., Cesbron, S., … Vernière, C. (2016). Using Ecology, Physiology, and Genomics to Understand Host Specificity in Xanthomonas. Annual Review of Phytopathology, 54(1), 163–29. https://doi.org/10.1146/annurev-phyto-080615-100147
Juan Sebastian Sánchez Ferro. (2020). CONSTRUCCIÓN DE PROMOTORES TRAMPA BASADOS EN EFECTORES TAL DE Xanthomonas axonopodis pv. manihotis. 1–135.
López, C. E., & Bernal, A. J. (2012). Cassava Bacterial Blight: Using Genomics for the Elucidation and Management of an Old Problem. Tropical Plant Biology, 5(1), 1–10. https://doi.org/10.1007/s12042-011-9092-3
Lopez, C., Restrepo, S., & Verdier, V. (2006). Limitaciones de la bacteriosis vascular de yuca: Nuevos avances. Acta Biol. Colomb, 1–25.
Lozano, J. C. (2008). Cassava Bacterial Blight: A Manageable Disease. Plant Disease, Vol. 70, p. 1089. https://doi.org/10.1094/pd-70-1089
McCallum, E. J., Anjanappa, R. B., & Gruissem, W. (2017). Tackling agriculturally relevant diseases in the staple crop cassava (Manihot esculenta). Current Opinion in Plant Biology, 38, 1–9. https://doi.org/10.1016/j.pbi.2017.04.008
Mücke, S., Reschke, M., Erkes, A., Schwietzer, C. A., Becker, S., Streubel, J., … Boch, J. (2019). Transcriptional reprogramming of rice cells by Xanthomonas oryzae tales. Frontiers in Plant Science, 10(February), 1–19. https://doi.org/10.3389/fpls.2019.00162
Noman, A., Aqeel, M., & Lou, Y. (2019). PRRs and NB-LRRs: From signal perception to activation of plant innate immunity. International Journal of Molecular Sciences, 20(8). https://doi.org/10.3390/ijms20081882
Pfeilmeier, S., Caly, D. L., & Malone, J. G. (2016). Bacterial pathogenesis of plants: future challenges from a microbial perspective: Challenges in Bacterial Molecular Plant Pathology. Molecular Plant Pathology, 17(8), 1–16. https://doi.org/10.1111/mpp.12427
Silva, M. S., Arraes, F. B. M., Campos, M. de A., Grossi-de-Sa, M., Fernandez, D., Cândido, E. de S., … Grossi-de-Sa, M. F. (2018). Review: Potential biotechnological assets related to plant immunity modulation applicable in engineering disease-resistant crops. Plant Science, 270(October 2017), 1–13. https://doi.org/10.1016/j.plantsci.2018.02.013
Silva, M. S., Arraes, F. B. M., Campos, M. de A., Grossi-de-Sa, M., Fernandez, D., Cândido, E. de S., … Grossi-de-Sa, M. F. (2018). Review: Potential biotechnological assets related to plant immunity modulation applicable in engineering disease-resistant crops. Plant Science, 270(October 2017), 1–13. https://doi.org/10.1016/j.plantsci.2018.02.013
Trujillo, C. A., Arias-Rojas, N., Poulin, L., Medina, C. A., Tapiero, A., Restrepo, S., … Bernal, A. J. (2014). Population typing of the causal agent of cassava bacterial blight in the Eastern Plains of Colombia using two types of molecular markers. BMC Microbiology, 14(1). https://doi.org/10.1186/1471-2180-14-161
Verdier, V. (2002). Bacteriosis Vascular ( o Añublo Bacteriano ) de la Yuca Causada por Xanthomonas axonopodis pv . manihotis. La Yuca En El Tercer Milenio: Sistemas Modernos de Producción, Procesamiento, Utilización y Comercialización., pp. 148–159.
Wan, W. L., Zhang, L., Pruitt, R., Zaidem, M., Brugman, R., Ma, X., … Nürnberger, T. (2019). Comparing Arabidopsis receptor kinase and receptor protein-mediated immune signaling reveals BIK1-dependent differences. New Phytologist, 221(4), 2080–2095. https://doi.org/10.1111/nph.15497
Wang, J., Wang, J., Hu, M., Wu, S., Qi, J., Wang, G., … Chai, J. (2019). Ligand-triggered allosteric ADP release primes a plant NLR complex. Science, 364(6435), 1–12. https://doi.org/10.1126/science.aav5868
White, F. F., & Yang, B. (2009). Host and Pathogen Factors Controlling the Rice-Xanthomonas oryzae Interaction. PLANT PHYSIOLOGY, 150(4), 1677–1686. https://doi.org/10.1104/pp.109.139360
Yu, X., Feng, B., He, P., & Shan, L. (2017). From Chaos to Harmony: Responses and Signaling upon Microbial Pattern Recognition. Annual Review of Phytopathology, 55(1), 109–137. https://doi.org/10.1146/annurev-phyto-080516-035649
Zárate, C. A. (2015). Diversity of TALE content in Xanthomonas axonopodis pv. manihotis strains is a valuable tool to improve target gene searching methodologies. Universidad de los Andes.
Zeng, X., Tian, D., Gu, K., Zhou, Z., Yang, X., Luo, Y., … Yin, Z. (2015). Genetic engineering of the Xa10 promoter for broad-spectrum and durable resistance to Xanthomonas oryzae pv. oryzae. Plant Biotechnology Journal, 13(7), 1–9. https://doi.org/10.1111/pbi.12342
Zhang, J., Yin, Z., & White, F. (2015). TAL effectors and the executor R genes. Frontiers in Plant Science, 6(AUG), 1–9. https://doi.org/10.3389/fpls.2015.00641
Zhang, M., Chiang, Y. H., Toruño, T. Y., Lee, D. H., Ma, M., Liang, X., … Coaker, G. (2018). The MAP4 Kinase SIK1 Ensures Robust Extracellular ROS Burst and Antibacterial Immunity in Plants. Cell Host and Microbe, 24(3), 379-391.e5. https://doi.org/10.1016/j.chom.2018.08.007
Zhang, X., Dodds, P. N., & Bernoux, M. (2017). What Do We Know About NOD-Like Receptors in Plant Immunity? Annual Review of Phytopathology, 55(1), 205–229. https://doi.org/10.1146/annurev-phyto-080516-035250
dc.identifier.instname.spa.fl_str_mv instname:Universidad Antonio Nariño
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dc.identifier.repourl.spa.fl_str_mv repourl:https://repositorio.uan.edu.co/
url http://repositorio.uan.edu.co/handle/123456789/1600
identifier_str_mv Aguilera, M. (2012). a Agroindustrial. Centro de Estudios Económicos Regionales (CEER) - Cartagena, 158. Retrieved from http://www.banrep.gov.co/docum/Lectura_finanzas/pdf/dtser_158.pdf
Boch, J., Bonas, U., & Lahaye, T. (2014). TAL effectors - pathogen strategies and plant resistance engineering. New Phytologist, 204(4), 823–832. https://doi.org/10.1111/nph.13015
Cohn, M., Bart, R. S., Shybut, M., Dahlbeck, D., Gomez, M., Morbitzer, R., … Staskawicz, B. J. (2014). Xanthomonas axonopodis virulence is promoted by a transcription activator-like effector - Mediated induction of a SWEET sugar transporter in Cassava. Molecular Plant-Microbe Interactions, 27(11), 1–13. https://doi.org/10.1094/MPMI-06-14-0161-R
Díaz, P. (2016). Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight ( CBB ). 1–187.
FAO. (2008). Yuca para la seguridad alimentaria y energética. Sala De Prensa, 3–4. Retrieved from http://www.fao.org/newsroom/es/news/2008/1000899/index.html
FAO. (2013). Noticias: La yuca tiene gran potencial como cultivo del siglo XXI. 28/05/2013, 3–5. Retrieved from http://www.fao.org/news/story/es/item/176821/icode/
FAO. (2017). FAOSTAT Database. Crops. Retrieved August 26, 2019, from http://www.fao.org/faostat/en/#data/QC
Grau, J., Wolf, A., Reschke, M., Bonas, U., Posch, S., & Boch, J. (2013). Computational Predictions Provide Insights into the Biology of TAL Effector Target Sites. PLoS Computational Biology, 9(3), 1–20. https://doi.org/10.1371/journal.pcbi.1002962
Hummel, A. W., Doyle, E. L., & Bogdanove, A. J. (2012). Addition of transcription activator-like effector binding sites to a pathogen strain-specific rice bacterial blight resistance gene makes it effective against additional strains and against bacterial leaf streak. New Phytologist, 195(4), 883–893. https://doi.org/10.1111/j.1469-8137.2012.04216.x
Jacques, M.-A., Arlat, M., Boulanger, A., Boureau, T., Carrère, S., Cesbron, S., … Vernière, C. (2016). Using Ecology, Physiology, and Genomics to Understand Host Specificity in Xanthomonas. Annual Review of Phytopathology, 54(1), 163–29. https://doi.org/10.1146/annurev-phyto-080615-100147
Juan Sebastian Sánchez Ferro. (2020). CONSTRUCCIÓN DE PROMOTORES TRAMPA BASADOS EN EFECTORES TAL DE Xanthomonas axonopodis pv. manihotis. 1–135.
López, C. E., & Bernal, A. J. (2012). Cassava Bacterial Blight: Using Genomics for the Elucidation and Management of an Old Problem. Tropical Plant Biology, 5(1), 1–10. https://doi.org/10.1007/s12042-011-9092-3
Lopez, C., Restrepo, S., & Verdier, V. (2006). Limitaciones de la bacteriosis vascular de yuca: Nuevos avances. Acta Biol. Colomb, 1–25.
Lozano, J. C. (2008). Cassava Bacterial Blight: A Manageable Disease. Plant Disease, Vol. 70, p. 1089. https://doi.org/10.1094/pd-70-1089
McCallum, E. J., Anjanappa, R. B., & Gruissem, W. (2017). Tackling agriculturally relevant diseases in the staple crop cassava (Manihot esculenta). Current Opinion in Plant Biology, 38, 1–9. https://doi.org/10.1016/j.pbi.2017.04.008
Mücke, S., Reschke, M., Erkes, A., Schwietzer, C. A., Becker, S., Streubel, J., … Boch, J. (2019). Transcriptional reprogramming of rice cells by Xanthomonas oryzae tales. Frontiers in Plant Science, 10(February), 1–19. https://doi.org/10.3389/fpls.2019.00162
Noman, A., Aqeel, M., & Lou, Y. (2019). PRRs and NB-LRRs: From signal perception to activation of plant innate immunity. International Journal of Molecular Sciences, 20(8). https://doi.org/10.3390/ijms20081882
Pfeilmeier, S., Caly, D. L., & Malone, J. G. (2016). Bacterial pathogenesis of plants: future challenges from a microbial perspective: Challenges in Bacterial Molecular Plant Pathology. Molecular Plant Pathology, 17(8), 1–16. https://doi.org/10.1111/mpp.12427
Silva, M. S., Arraes, F. B. M., Campos, M. de A., Grossi-de-Sa, M., Fernandez, D., Cândido, E. de S., … Grossi-de-Sa, M. F. (2018). Review: Potential biotechnological assets related to plant immunity modulation applicable in engineering disease-resistant crops. Plant Science, 270(October 2017), 1–13. https://doi.org/10.1016/j.plantsci.2018.02.013
Trujillo, C. A., Arias-Rojas, N., Poulin, L., Medina, C. A., Tapiero, A., Restrepo, S., … Bernal, A. J. (2014). Population typing of the causal agent of cassava bacterial blight in the Eastern Plains of Colombia using two types of molecular markers. BMC Microbiology, 14(1). https://doi.org/10.1186/1471-2180-14-161
Verdier, V. (2002). Bacteriosis Vascular ( o Añublo Bacteriano ) de la Yuca Causada por Xanthomonas axonopodis pv . manihotis. La Yuca En El Tercer Milenio: Sistemas Modernos de Producción, Procesamiento, Utilización y Comercialización., pp. 148–159.
Wan, W. L., Zhang, L., Pruitt, R., Zaidem, M., Brugman, R., Ma, X., … Nürnberger, T. (2019). Comparing Arabidopsis receptor kinase and receptor protein-mediated immune signaling reveals BIK1-dependent differences. New Phytologist, 221(4), 2080–2095. https://doi.org/10.1111/nph.15497
Wang, J., Wang, J., Hu, M., Wu, S., Qi, J., Wang, G., … Chai, J. (2019). Ligand-triggered allosteric ADP release primes a plant NLR complex. Science, 364(6435), 1–12. https://doi.org/10.1126/science.aav5868
White, F. F., & Yang, B. (2009). Host and Pathogen Factors Controlling the Rice-Xanthomonas oryzae Interaction. PLANT PHYSIOLOGY, 150(4), 1677–1686. https://doi.org/10.1104/pp.109.139360
Yu, X., Feng, B., He, P., & Shan, L. (2017). From Chaos to Harmony: Responses and Signaling upon Microbial Pattern Recognition. Annual Review of Phytopathology, 55(1), 109–137. https://doi.org/10.1146/annurev-phyto-080516-035649
Zárate, C. A. (2015). Diversity of TALE content in Xanthomonas axonopodis pv. manihotis strains is a valuable tool to improve target gene searching methodologies. Universidad de los Andes.
Zeng, X., Tian, D., Gu, K., Zhou, Z., Yang, X., Luo, Y., … Yin, Z. (2015). Genetic engineering of the Xa10 promoter for broad-spectrum and durable resistance to Xanthomonas oryzae pv. oryzae. Plant Biotechnology Journal, 13(7), 1–9. https://doi.org/10.1111/pbi.12342
Zhang, J., Yin, Z., & White, F. (2015). TAL effectors and the executor R genes. Frontiers in Plant Science, 6(AUG), 1–9. https://doi.org/10.3389/fpls.2015.00641
Zhang, M., Chiang, Y. H., Toruño, T. Y., Lee, D. H., Ma, M., Liang, X., … Coaker, G. (2018). The MAP4 Kinase SIK1 Ensures Robust Extracellular ROS Burst and Antibacterial Immunity in Plants. Cell Host and Microbe, 24(3), 379-391.e5. https://doi.org/10.1016/j.chom.2018.08.007
Zhang, X., Dodds, P. N., & Bernoux, M. (2017). What Do We Know About NOD-Like Receptors in Plant Immunity? Annual Review of Phytopathology, 55(1), 205–229. https://doi.org/10.1146/annurev-phyto-080516-035250
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institution Universidad Antonio Nariño
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spelling Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Díaz Tatis, Paula AlejandraSanchez Ferro, Juan SebastianSantamaría Rodríguez, Brian David2021-02-22T14:28:01Z2021-02-22T14:28:01Z2020-06-03http://repositorio.uan.edu.co/handle/123456789/1600Aguilera, M. (2012). a Agroindustrial. Centro de Estudios Económicos Regionales (CEER) - Cartagena, 158. Retrieved from http://www.banrep.gov.co/docum/Lectura_finanzas/pdf/dtser_158.pdfBoch, J., Bonas, U., & Lahaye, T. (2014). TAL effectors - pathogen strategies and plant resistance engineering. New Phytologist, 204(4), 823–832. https://doi.org/10.1111/nph.13015Cohn, M., Bart, R. S., Shybut, M., Dahlbeck, D., Gomez, M., Morbitzer, R., … Staskawicz, B. J. (2014). Xanthomonas axonopodis virulence is promoted by a transcription activator-like effector - Mediated induction of a SWEET sugar transporter in Cassava. Molecular Plant-Microbe Interactions, 27(11), 1–13. https://doi.org/10.1094/MPMI-06-14-0161-RDíaz, P. (2016). Transference of RXam2 and Bs2 genes to confer resistance against cassava bacterial blight ( CBB ). 1–187.FAO. (2008). Yuca para la seguridad alimentaria y energética. Sala De Prensa, 3–4. Retrieved from http://www.fao.org/newsroom/es/news/2008/1000899/index.htmlFAO. (2013). Noticias: La yuca tiene gran potencial como cultivo del siglo XXI. 28/05/2013, 3–5. Retrieved from http://www.fao.org/news/story/es/item/176821/icode/FAO. (2017). FAOSTAT Database. Crops. Retrieved August 26, 2019, from http://www.fao.org/faostat/en/#data/QCGrau, J., Wolf, A., Reschke, M., Bonas, U., Posch, S., & Boch, J. (2013). Computational Predictions Provide Insights into the Biology of TAL Effector Target Sites. PLoS Computational Biology, 9(3), 1–20. https://doi.org/10.1371/journal.pcbi.1002962Hummel, A. W., Doyle, E. L., & Bogdanove, A. J. (2012). Addition of transcription activator-like effector binding sites to a pathogen strain-specific rice bacterial blight resistance gene makes it effective against additional strains and against bacterial leaf streak. New Phytologist, 195(4), 883–893. https://doi.org/10.1111/j.1469-8137.2012.04216.xJacques, M.-A., Arlat, M., Boulanger, A., Boureau, T., Carrère, S., Cesbron, S., … Vernière, C. (2016). Using Ecology, Physiology, and Genomics to Understand Host Specificity in Xanthomonas. Annual Review of Phytopathology, 54(1), 163–29. https://doi.org/10.1146/annurev-phyto-080615-100147Juan Sebastian Sánchez Ferro. (2020). CONSTRUCCIÓN DE PROMOTORES TRAMPA BASADOS EN EFECTORES TAL DE Xanthomonas axonopodis pv. manihotis. 1–135.López, C. E., & Bernal, A. J. (2012). Cassava Bacterial Blight: Using Genomics for the Elucidation and Management of an Old Problem. Tropical Plant Biology, 5(1), 1–10. https://doi.org/10.1007/s12042-011-9092-3Lopez, C., Restrepo, S., & Verdier, V. (2006). Limitaciones de la bacteriosis vascular de yuca: Nuevos avances. Acta Biol. Colomb, 1–25.Lozano, J. C. (2008). Cassava Bacterial Blight: A Manageable Disease. Plant Disease, Vol. 70, p. 1089. https://doi.org/10.1094/pd-70-1089McCallum, E. J., Anjanappa, R. B., & Gruissem, W. (2017). Tackling agriculturally relevant diseases in the staple crop cassava (Manihot esculenta). Current Opinion in Plant Biology, 38, 1–9. https://doi.org/10.1016/j.pbi.2017.04.008Mücke, S., Reschke, M., Erkes, A., Schwietzer, C. A., Becker, S., Streubel, J., … Boch, J. (2019). Transcriptional reprogramming of rice cells by Xanthomonas oryzae tales. Frontiers in Plant Science, 10(February), 1–19. https://doi.org/10.3389/fpls.2019.00162Noman, A., Aqeel, M., & Lou, Y. (2019). 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Annual Review of Phytopathology, 55(1), 205–229. https://doi.org/10.1146/annurev-phyto-080516-035250instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/Xanthomonas phaseoli pv. manihotis (Xpm) is the causal agent of cassava bacterial blight, a disease that can generate considerable losses in this crop. This bacterium possesses effector proteins that are injected into the host cell through the type-three secretion system and that function as virulence factors. Some of these effectors, called TALEs (Transcription-Activator Like Effectors), enter the nucleus of host cells, bind specifically to host DNA sequences, called effector-binding elements (EBEs), and act as transcription factors to modulate host gene expression. The knowledge of the mode of action for TALEs in species as rice has allowed the development of biotechnological strategies to generate broad-spectrum resistance to pathogens. In this study, a trap promoter was developed that is activated by three predominant TALEs in Xpm strains that allow the expression of the resistance gene RXam2(MHV). Our results show that when TALEs 14, 20 and 22 are co-infiltrated with the synthetic promoter that contains the EBEs for this TALEs a hypersensitive response is produced in the infiltrated area in leaves of N. tabacum. These results represent an important achivement for the use of this strategy in future cassava breeding programs.Xanthomonas phaseoli pv. manihotis (Xpm) es el agente causal de la bacteriosis vascular de la yuca, enfermedad que puede generar pérdidas considerables en este cultivo. Esta bacteria posee proteínas efectoras que son inyectadas al interior de la célula hospedera a través del sistema de secreción tipo tres y que funcionan como factores de virulencia. Los efectores llamados TALEs (del inglés, Transcription-Activator Like Effector), ingresan al núcleo de las células hospederas, se unen de manera específica a las secuencias de ADN del hospedero, denominadas elementos de unión al efector (EBE) y actúan como factores de transcripción para modular la expresión de genes del hospedero. El conocimiento del modo de acción de los TALEs ha permitido desarrollar estrategias biotecnológicas para generar resistencia de amplio espectro a patógenos de plantas. En este estudio se elaboró un promotor trampa activado por tres TALEs predominantes en cepas de Xpm para inducir la expresión del gen de resistencia RXam2(MHV). Los resultados de los experimentos de expresión transitoria muestran que la co-infiltración de los TALEs 14, 20 y 22 junto con el promotor trampa que contiene los EBEs para estos tres efectores causan una respuesta hipersensible en el área infiltrada en hojas de N.tabacum.Bioquímico(a)PregradoPresencialspaUniversidad Antonio NariñoBioquímicaFacultad de CienciasBogotá - Circunvalarbacteriosis vascular de la yuca, promotor trampa, respuesta hipersensible.cassava bacterial blight, trap promoter, hypersensitive response.Inducción de la expresión del gen de resistencia de yuca RXam2 mediante un promotor inducible por tres efectores tipo tal de xanthomonas phaseoli pv. manihotisTrabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85ORIGINAL2020AutorizacióndeAutores.pdf2020AutorizacióndeAutores.pdfAutorización autores Brian Santamaríaapplication/pdf56121https://repositorio.uan.edu.co/bitstreams/ce2310f7-22ef-400b-b2ca-32d68ac837c1/download880c071d270520bff2df6798f6ba0539MD512020BrianDavidSantamariaRodríguez.pdf2020BrianDavidSantamariaRodríguez.pdfTrabajo de grado Brian Santamaríaapplication/pdf656099https://repositorio.uan.edu.co/bitstreams/d3f0d841-72aa-4b1b-afc4-9a7e23004934/downloade1ccc59f624fd84e28d91f3202cd1437MD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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