Proliferación celular en andamios de fibrina, agarosa y fibrina-agarosa: revisión narrativa

Propia

Autores:
Cortés Múnera, Yessica María
Lacera Perez, Ovanys
Pedraza Corrales, Kelly Johanna
Tipo de recurso:
Trabajo de grado de pregrado
Fecha de publicación:
2020
Institución:
Universidad Antonio Nariño
Repositorio:
Repositorio UAN
Idioma:
spa
OAI Identifier:
oai:repositorio.uan.edu.co:123456789/2619
Acceso en línea:
http://repositorio.uan.edu.co/handle/123456789/2619
Palabra clave:
fibrina, agarosa, fibrina-agarosa, proliferación celular, hidrogeles.
Fibrin, agarose, fibrin-agarose, cell proliferation, scaffolds, hydrogels.
Rights
openAccess
License
Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
id UAntonioN2_436a77a87b4c965faba72b7652384939
oai_identifier_str oai:repositorio.uan.edu.co:123456789/2619
network_acronym_str UAntonioN2
network_name_str Repositorio UAN
repository_id_str
dc.title.es_ES.fl_str_mv Proliferación celular en andamios de fibrina, agarosa y fibrina-agarosa: revisión narrativa
title Proliferación celular en andamios de fibrina, agarosa y fibrina-agarosa: revisión narrativa
spellingShingle Proliferación celular en andamios de fibrina, agarosa y fibrina-agarosa: revisión narrativa
fibrina, agarosa, fibrina-agarosa, proliferación celular, hidrogeles.
Fibrin, agarose, fibrin-agarose, cell proliferation, scaffolds, hydrogels.
title_short Proliferación celular en andamios de fibrina, agarosa y fibrina-agarosa: revisión narrativa
title_full Proliferación celular en andamios de fibrina, agarosa y fibrina-agarosa: revisión narrativa
title_fullStr Proliferación celular en andamios de fibrina, agarosa y fibrina-agarosa: revisión narrativa
title_full_unstemmed Proliferación celular en andamios de fibrina, agarosa y fibrina-agarosa: revisión narrativa
title_sort Proliferación celular en andamios de fibrina, agarosa y fibrina-agarosa: revisión narrativa
dc.creator.fl_str_mv Cortés Múnera, Yessica María
Lacera Perez, Ovanys
Pedraza Corrales, Kelly Johanna
dc.contributor.advisor.spa.fl_str_mv Alfonso Rodriguez, Camilo
González Colmenares, Gretel
dc.contributor.author.spa.fl_str_mv Cortés Múnera, Yessica María
Lacera Perez, Ovanys
Pedraza Corrales, Kelly Johanna
dc.subject.es_ES.fl_str_mv fibrina, agarosa, fibrina-agarosa, proliferación celular, hidrogeles.
topic fibrina, agarosa, fibrina-agarosa, proliferación celular, hidrogeles.
Fibrin, agarose, fibrin-agarose, cell proliferation, scaffolds, hydrogels.
dc.subject.keyword.es_ES.fl_str_mv Fibrin, agarose, fibrin-agarose, cell proliferation, scaffolds, hydrogels.
description Propia
publishDate 2020
dc.date.issued.spa.fl_str_mv 2020-11-19
dc.date.accessioned.none.fl_str_mv 2021-03-03T22:24:48Z
dc.date.available.none.fl_str_mv 2021-03-03T22:24:48Z
dc.type.spa.fl_str_mv Trabajo de grado (Pregrado y/o Especialización)
dc.type.coar.spa.fl_str_mv http://purl.org/coar/resource_type/c_7a1f
dc.type.coarversion.none.fl_str_mv http://purl.org/coar/version/c_970fb48d4fbd8a85
format http://purl.org/coar/resource_type/c_7a1f
dc.identifier.uri.none.fl_str_mv http://repositorio.uan.edu.co/handle/123456789/2619
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dc.identifier.instname.spa.fl_str_mv instname:Universidad Antonio Nariño
dc.identifier.reponame.spa.fl_str_mv reponame:Repositorio Institucional UAN
dc.identifier.repourl.spa.fl_str_mv repourl:https://repositorio.uan.edu.co/
url http://repositorio.uan.edu.co/handle/123456789/2619
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spelling Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Alfonso Rodriguez, CamiloGonzález Colmenares, GretelCortés Múnera, Yessica MaríaLacera Perez, OvanysPedraza Corrales, Kelly Johanna0000-0001-7243-9272http://scienti.colciencias.gov.co:8081/cvlac/jsp/report-index.jsp10473746611090455555106771553580541239521446722021-03-03T22:24:48Z2021-03-03T22:24:48Z2020-11-19http://repositorio.uan.edu.co/handle/123456789/26191. Achilleas T, Spyros S, Chrysostomi G, Karamanos K. Extracellular matrix structure. Adv Drug Deliv Rev. 2016; 97: 4-27.2. Kant G. Interacción entre las células y su ambiente. En: Javier de León Fagra. Biología molecular y celular-Conceptos y experimentos. Sexta edición. México D.F.: McGRAW-HILL INTERAMERICANA EDITORES;2011. p. 230.3. 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Crit Rev Ther Drug Carrier Syst. 2012; 29(1): 1-63.instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/PropiaIntroduction: Scaffolds must be designed to replicate the native tissue architecture in vitro, so that cells adhere, spread, proliferate, differentiate and mature, in a similar way to what they do in vivo. The objective of this review was to analyze the scientific evidence available on cell proliferation in scaffolds based on fibrin, agarose and fibrin-agarose mixture biomaterials. Materials and methods: Narrative review, bibliographic search of the last 5 years published in Medline-PubMed and Web of Science. during the period of August and September 2020. Inclusion criteria: 1). Experimental studies in vitro, in vivo, ex vivo, 2) Studies that will evaluate cell proliferation in fibrin, agarose and fibrin-agarose mixture scaffolds, 3). Preclinical trials, 4). Bibliographic reviews, 5). Articles published in English and Spanish, 6). Documents published between 2016 and 2020. Thematic analysis was carried out by topics in each scaffold: cell types, types of cell proliferation assays, cell proliferation evaluation times and cell proliferation results. Results: 40 articles, 21 for fibrin, 9 for agarose, 10 for fibrin-agarose. The cells most used in the studies of the three scaffolds were mesenchymal stem cells of various origins. The most widely used types of assay were DNA quantification, the Ki67 proliferation marker and the cellular proliferation nuclear antigen (PCNA). The most used cell proliferation evaluation time period was 1 to 7 days. The qualitative proliferation results were more prevalent than the quantitative ones, finding great diversity in the reports. Conclusions: It is reported that the three scaffolds object of the review in general have the ability to promote cell proliferation.Introducción: Los andamios debe estar diseñado para replicar in vitro la arquitectura del tejido nativo, para que las células se adhieran, se extiendan, proliferen, se diferencien y maduren, de manera similar a lo que hacen in vivo. El objetivo de esta revisión fue analizar la evidencia científica disponible sobre la proliferación celular en andamios basados en biomateriales de fibrina, agarosa y mezcla de fibrina-agarosa. Materiales y métodos: Revisión narrativa, búsqueda bibliográfica de los últimos 5 años publicada en Medline-PubMed y Web of Science. durante el periodo de agosto y septiembre de 2020. Criterios de inclusión :1). Estudios experimentales in vitro, in vivo, ex vivo, 2) Estudios que evaluaran la proliferación celular en andamios de fibrina, agarosa y mezcla de fibrina-agarosa, 3). Ensayos preclínicos, 4). Revisiones bibliográficas, 5). Artículos publicados en inglés y español, 6). Documentos publicados entre 2016 y 2020. Se realizó análisis temático por tópicos en cada andamio: Tipos celulares, tipos de ensayos de proliferación celular, tiempos de evaluación de la proliferación celular y resultados de la proliferación celular. Resultados: 40 artículos, 21 para fibrina, 9 para agarosa, 10 para fibrina-agarosa. Las células más usadas en los estudios de los tres andamios fueron las células madre mesenquimales de diversos orígenes. Los tipos de ensayo más utilizados fueron la cuantificación de ADN, el marcador de proliferación Ki67 y el antígeno nuclear de proliferación celular (PCNA). El periodo de tiempo de evaluación de la proliferación celular más usado fue de 1 a 7 días. Los resultados de la proliferación cualitativos fueron más prevalentes que los cuantitativos, encontrándose gran diversidad en los reportes. Conclusiones: Se reporta que los tres andamios objeto de la revisión en general tienen la capacidad de promover la proliferación celular.Especialista en PeriodonciaEspecializaciónPresencialspaUniversidad Antonio NariñoEspecialización en PeriodonciaFacultad de OdontologíaBogotá - Circunvalarfibrina, agarosa, fibrina-agarosa, proliferación celular, hidrogeles.Fibrin, agarose, fibrin-agarose, cell proliferation, scaffolds, hydrogels.Proliferación celular en andamios de fibrina, agarosa y fibrina-agarosa: revisión narrativaTrabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.uan.edu.co/bitstreams/bfdd2357-8dcc-4c73-a592-4f323a6a02a2/download9868ccc48a14c8d591352b6eaf7f6239MD55LICENSElicense.txtlicense.txttext/plain; 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