Determinación del contenido de polifenoles del extracto y fracciones de Bursera tomentosa, y su efecto en la viabilidad celular sobre células SiHa.

Research on natural products is constantly searching for molecules with biological activity that may represent an alternative to modern treatments, leading to the identification of compounds in littlestudied plant species, such as the Bursera tomentosa species. Where it was determined qualitatively,...

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Autores:: Ortiz Perdomo, Sebastian Camilo

Tipo de recurso:: Trabajo de grado de pregrado

Fecha de publicación:: 2023

Institución:: Universidad Antonio Nariño

Repositorio:: Repositorio UAN

Idioma:: spa

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dc.title.es_ES.fl_str_mv	Determinación del contenido de polifenoles del extracto y fracciones de Bursera tomentosa, y su efecto en la viabilidad celular sobre células SiHa.
title	Determinación del contenido de polifenoles del extracto y fracciones de Bursera tomentosa, y su efecto en la viabilidad celular sobre células SiHa.
spellingShingle	Determinación del contenido de polifenoles del extracto y fracciones de Bursera tomentosa, y su efecto en la viabilidad celular sobre células SiHa. Bursera tomentosa extracto polifenoles Folin-Ciocalteu, viabilidad celular y SiHa Bursera tomentosa extract, polyphenols Folin – Ciocalteu, cell viability and SiHa
title_short	Determinación del contenido de polifenoles del extracto y fracciones de Bursera tomentosa, y su efecto en la viabilidad celular sobre células SiHa.
title_full	Determinación del contenido de polifenoles del extracto y fracciones de Bursera tomentosa, y su efecto en la viabilidad celular sobre células SiHa.
title_fullStr	Determinación del contenido de polifenoles del extracto y fracciones de Bursera tomentosa, y su efecto en la viabilidad celular sobre células SiHa.
title_full_unstemmed	Determinación del contenido de polifenoles del extracto y fracciones de Bursera tomentosa, y su efecto en la viabilidad celular sobre células SiHa.
title_sort	Determinación del contenido de polifenoles del extracto y fracciones de Bursera tomentosa, y su efecto en la viabilidad celular sobre células SiHa.
dc.creator.fl_str_mv	Ortiz Perdomo, Sebastian Camilo
dc.contributor.advisor.spa.fl_str_mv	Rojas Sepúlveda., Andrés Mauricio Durango García., Clara Juliana
dc.contributor.author.spa.fl_str_mv	Ortiz Perdomo, Sebastian Camilo
dc.subject.es_ES.fl_str_mv	Bursera tomentosa extracto polifenoles Folin-Ciocalteu, viabilidad celular y SiHa
topic	Bursera tomentosa extracto polifenoles Folin-Ciocalteu, viabilidad celular y SiHa Bursera tomentosa extract, polyphenols Folin – Ciocalteu, cell viability and SiHa
dc.subject.keyword.es_ES.fl_str_mv	Bursera tomentosa extract, polyphenols Folin – Ciocalteu, cell viability and SiHa
description	Research on natural products is constantly searching for molecules with biological activity that may represent an alternative to modern treatments, leading to the identification of compounds in littlestudied plant species, such as the Bursera tomentosa species. Where it was determined qualitatively, by means of phytochemical screening, which has triterpenes, flavonoids, phenols, tannins and alkaloids in its chemical profile. It was found that the polyphenol content in the crude extract is 129.49 ± 11 mg/g equivalents of gallic acid, through the Folin-Ciocalteau test. In the evaluation of biological activity, the extract and the ethyl acetate, ethanol and aqueous fractions, against the SiHa cell line, managed to reduce the percentage of survival below 50%, in contrast to the effect on the vero cell line, where this effect was only evidenced for the ethyl acetate fraction
publishDate	2023
dc.date.accessioned.none.fl_str_mv	2023-10-26T21:19:16Z
dc.date.available.none.fl_str_mv	2023-10-26T21:19:16Z
dc.date.issued.spa.fl_str_mv	2023-07-07
dc.type.spa.fl_str_mv	Trabajo de grado (Pregrado y/o Especialización)
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dc.identifier.uri.none.fl_str_mv	http://repositorio.uan.edu.co/handle/123456789/8799
dc.identifier.bibliographicCitation.spa.fl_str_mv	Ahmadiankia, N. (2020). In vitro and in vivo studies of cancer cell behavior under nutrient deprivation. Cell Biology International, 44(8), 1588–1597. https://doi.org/10.1002/CBIN.11368 Aliyazcoglu, R., Sahin, H., Erturk, O., Ulusoy, E., & Kolayli, S. (2012). Properties of Phenolic Composition and Biological Activity of Propolis from Turkey. Https://Doi.Org/10.1080/10942912.2010.551312, 16(2), 277–287. https://doi.org/10.1080/10942912.2010.551312 Ardalani, H., Avan, A., & Ghayour-Mobarhan, M. (2017). Podophyllotoxin: a novel potential natural anticancer agent. Avicenna Journal of Phytomedicine, 7(4), 285. /pmc/articles/PMC5580867/ Aviram, M., Dornfeld, L., Rosenblat, M., Volkova, N., Kaplan, M., Coleman, R., Hayek, T., Presser, D., & Fuhrman, B. (2000). Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E-deficient mice. The American Journal of Clinical Nutrition, 71(5), 1062–1076. https://doi.org/10.1093/AJCN/71.5.1062 Bae, S. H., & Suh, H. J. (2007). Antioxidant activities of five different mulberry cultivars in Korea. Lwt, 40(6), 955–962. https://doi.org/10.1016/j.lwt.2006.06.007 Bah, M., Gutiérrez-Avella, D. M., Mendoza, S., Rodríguez-López, V., & Castañeda-Moreno, R. (2014). Chemical constituents and antioxidant activity of extracts obtained from branch bark of bursera simaruba. Boletin Latinoamericano y Del Caribe de Plantas Medicinales y Aromaticas, 13(6), 527–536 Barreto, J. J. J. (2009). Evaluación preliminar de la actividad antiinflamatoria de las fracciones obtenidas de los extractos en petrol y en etanol de hojas y corteza de la planta. In Tesis (Vol. 1) Belew, A. A., Mariam, G. G. /, Hana, W. /, Meshesha, D. S., Akele, M. L., Shumuye Meshesha, D., & Legese Akele, M. (2021). Evaluation of Total Phenolic, Total Flavonoid Content And Antioxidant Activity of Rhus Vulgaris. https://doi.org/10.21203/RS.3.RS-1207023/V1 Buckner, C. A., Lafrenie, R. M., Dénommée, J. A., Caswell, J. M., Want, D. A., Gan, G. G., Leong, Y. C., Bee, P. C., Chin, E., Teh, A. K. H., Picco, S., Villegas, L., Tonelli, F., Merlo, M., Rigau, J., Diaz, D., Masuelli, M., Korrapati, S., Kurra, P., … Mathijssen, R. H. J. (2016). Phenolic Compounds: Classification, Chemistry, and Updated Techniques of Analysis and Synthesis. Intech, 11(tourism), 13. https://www.intechopen.com/books/advanced-biometrictechnologies/liveness-detection-in-biometrics Camargo, J. E. R., Alfonso, A. N. T., Rojas-Rozo, R. A., Castro, C. de, & Murcia, T. R. de. (2010). Efectos citotoxicos in vitro de extractos y fracciones de Bursera tomentosa (Jacq.) Triana & Planch., Burseraceae, frente a lineas celulares tumorales humanos. Revista Brasileira de Farmacognosia, 20(4), 588–593. https://doi.org/10.1590/S0102-695X2010000400019
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identifier_str_mv	Ahmadiankia, N. (2020). In vitro and in vivo studies of cancer cell behavior under nutrient deprivation. Cell Biology International, 44(8), 1588–1597. https://doi.org/10.1002/CBIN.11368 Aliyazcoglu, R., Sahin, H., Erturk, O., Ulusoy, E., & Kolayli, S. (2012). Properties of Phenolic Composition and Biological Activity of Propolis from Turkey. Https://Doi.Org/10.1080/10942912.2010.551312, 16(2), 277–287. https://doi.org/10.1080/10942912.2010.551312 Ardalani, H., Avan, A., & Ghayour-Mobarhan, M. (2017). Podophyllotoxin: a novel potential natural anticancer agent. Avicenna Journal of Phytomedicine, 7(4), 285. /pmc/articles/PMC5580867/ Aviram, M., Dornfeld, L., Rosenblat, M., Volkova, N., Kaplan, M., Coleman, R., Hayek, T., Presser, D., & Fuhrman, B. (2000). Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E-deficient mice. The American Journal of Clinical Nutrition, 71(5), 1062–1076. https://doi.org/10.1093/AJCN/71.5.1062 Bae, S. H., & Suh, H. J. (2007). Antioxidant activities of five different mulberry cultivars in Korea. Lwt, 40(6), 955–962. https://doi.org/10.1016/j.lwt.2006.06.007 Bah, M., Gutiérrez-Avella, D. M., Mendoza, S., Rodríguez-López, V., & Castañeda-Moreno, R. (2014). Chemical constituents and antioxidant activity of extracts obtained from branch bark of bursera simaruba. Boletin Latinoamericano y Del Caribe de Plantas Medicinales y Aromaticas, 13(6), 527–536 Barreto, J. J. J. (2009). Evaluación preliminar de la actividad antiinflamatoria de las fracciones obtenidas de los extractos en petrol y en etanol de hojas y corteza de la planta. In Tesis (Vol. 1) Belew, A. A., Mariam, G. G. /, Hana, W. /, Meshesha, D. S., Akele, M. L., Shumuye Meshesha, D., & Legese Akele, M. (2021). Evaluation of Total Phenolic, Total Flavonoid Content And Antioxidant Activity of Rhus Vulgaris. https://doi.org/10.21203/RS.3.RS-1207023/V1 Buckner, C. A., Lafrenie, R. M., Dénommée, J. A., Caswell, J. M., Want, D. A., Gan, G. G., Leong, Y. C., Bee, P. C., Chin, E., Teh, A. K. H., Picco, S., Villegas, L., Tonelli, F., Merlo, M., Rigau, J., Diaz, D., Masuelli, M., Korrapati, S., Kurra, P., … Mathijssen, R. H. J. (2016). Phenolic Compounds: Classification, Chemistry, and Updated Techniques of Analysis and Synthesis. Intech, 11(tourism), 13. https://www.intechopen.com/books/advanced-biometrictechnologies/liveness-detection-in-biometrics Camargo, J. E. R., Alfonso, A. N. T., Rojas-Rozo, R. A., Castro, C. de, & Murcia, T. R. de. (2010). Efectos citotoxicos in vitro de extractos y fracciones de Bursera tomentosa (Jacq.) Triana & Planch., Burseraceae, frente a lineas celulares tumorales humanos. Revista Brasileira de Farmacognosia, 20(4), 588–593. https://doi.org/10.1590/S0102-695X2010000400019 instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/
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dc.publisher.spa.fl_str_mv	Universidad Antonio Nariño
dc.publisher.program.spa.fl_str_mv	Bioquímica
dc.publisher.faculty.spa.fl_str_mv	Facultad de Ciencias
dc.publisher.campus.spa.fl_str_mv	Bogotá - Circunvalar
institution	Universidad Antonio Nariño
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spelling	Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Rojas Sepúlveda., Andrés MauricioDurango García., Clara JulianaOrtiz Perdomo, Sebastian Camilo118218247882023-10-26T21:19:16Z2023-10-26T21:19:16Z2023-07-07http://repositorio.uan.edu.co/handle/123456789/8799Ahmadiankia, N. (2020). In vitro and in vivo studies of cancer cell behavior under nutrient deprivation. Cell Biology International, 44(8), 1588–1597. https://doi.org/10.1002/CBIN.11368Aliyazcoglu, R., Sahin, H., Erturk, O., Ulusoy, E., & Kolayli, S. (2012). Properties of Phenolic Composition and Biological Activity of Propolis from Turkey. Https://Doi.Org/10.1080/10942912.2010.551312, 16(2), 277–287. https://doi.org/10.1080/10942912.2010.551312Ardalani, H., Avan, A., & Ghayour-Mobarhan, M. (2017). Podophyllotoxin: a novel potential natural anticancer agent. Avicenna Journal of Phytomedicine, 7(4), 285. /pmc/articles/PMC5580867/Aviram, M., Dornfeld, L., Rosenblat, M., Volkova, N., Kaplan, M., Coleman, R., Hayek, T., Presser, D., & Fuhrman, B. (2000). Pomegranate juice consumption reduces oxidative stress, atherogenic modifications to LDL, and platelet aggregation: studies in humans and in atherosclerotic apolipoprotein E-deficient mice. The American Journal of Clinical Nutrition, 71(5), 1062–1076. https://doi.org/10.1093/AJCN/71.5.1062Bae, S. H., & Suh, H. J. (2007). Antioxidant activities of five different mulberry cultivars in Korea. Lwt, 40(6), 955–962. https://doi.org/10.1016/j.lwt.2006.06.007Bah, M., Gutiérrez-Avella, D. M., Mendoza, S., Rodríguez-López, V., & Castañeda-Moreno, R. (2014). Chemical constituents and antioxidant activity of extracts obtained from branch bark of bursera simaruba. Boletin Latinoamericano y Del Caribe de Plantas Medicinales y Aromaticas, 13(6), 527–536Barreto, J. J. J. (2009). Evaluación preliminar de la actividad antiinflamatoria de las fracciones obtenidas de los extractos en petrol y en etanol de hojas y corteza de la planta. In Tesis (Vol. 1)Belew, A. A., Mariam, G. G. /, Hana, W. /, Meshesha, D. S., Akele, M. L., Shumuye Meshesha, D., & Legese Akele, M. (2021). Evaluation of Total Phenolic, Total Flavonoid Content And Antioxidant Activity of Rhus Vulgaris. https://doi.org/10.21203/RS.3.RS-1207023/V1Buckner, C. A., Lafrenie, R. M., Dénommée, J. A., Caswell, J. M., Want, D. A., Gan, G. G., Leong, Y. C., Bee, P. C., Chin, E., Teh, A. K. H., Picco, S., Villegas, L., Tonelli, F., Merlo, M., Rigau, J., Diaz, D., Masuelli, M., Korrapati, S., Kurra, P., … Mathijssen, R. H. J. (2016). Phenolic Compounds: Classification, Chemistry, and Updated Techniques of Analysis and Synthesis. Intech, 11(tourism), 13. https://www.intechopen.com/books/advanced-biometrictechnologies/liveness-detection-in-biometricsCamargo, J. E. R., Alfonso, A. N. T., Rojas-Rozo, R. A., Castro, C. de, & Murcia, T. R. de. (2010). Efectos citotoxicos in vitro de extractos y fracciones de Bursera tomentosa (Jacq.) Triana & Planch., Burseraceae, frente a lineas celulares tumorales humanos. Revista Brasileira de Farmacognosia, 20(4), 588–593. https://doi.org/10.1590/S0102-695X2010000400019instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/Research on natural products is constantly searching for molecules with biological activity that may represent an alternative to modern treatments, leading to the identification of compounds in littlestudied plant species, such as the Bursera tomentosa species. Where it was determined qualitatively, by means of phytochemical screening, which has triterpenes, flavonoids, phenols, tannins and alkaloids in its chemical profile. It was found that the polyphenol content in the crude extract is 129.49 ± 11 mg/g equivalents of gallic acid, through the Folin-Ciocalteau test. In the evaluation of biological activity, the extract and the ethyl acetate, ethanol and aqueous fractions, against the SiHa cell line, managed to reduce the percentage of survival below 50%, in contrast to the effect on the vero cell line, where this effect was only evidenced for the ethyl acetate fractionLa investigación de productos naturales se mantiene en constante búsqueda de moléculas con actividad biológica, que puedan representar una alternativa a los tratamientos modernos, llevando a la identificación de compuestos, en especies de plantas poco estudiadas, como es el caso de la especie Bursera tomentosa. Donde se determinó de forma cualitativa, la presencia de compuestos de tipo triterpenos, flavonoides, fenoles, taninos y alcaloides. Se encontró que el contenido de polifenoles en el extracto crudo es de 129.49 ± 11 mg/g equivalentes de ácido gálico, a través de la prueba FolinCiocalteau. En la evaluación de actividad biológica, el extracto y las fracciones de acetato de etilo, etanol y acuosa, frente a la línea celular SiHa, lograron reducir el porcentaje de supervivencia por debajo del 50%, en contraste al efecto sobre la línea celular vero, donde este efecto solo se evidencio para la fracción acetato de etiloBioquímico(a)PregradoPresencialInvestigaciónspaUniversidad Antonio NariñoBioquímicaFacultad de CienciasBogotá - CircunvalarBursera tomentosaextractopolifenolesFolin-Ciocalteu, viabilidad celular y SiHaBursera tomentosaextract, polyphenolsFolin – Ciocalteu, cell viability and SiHaDeterminación del contenido de polifenoles del extracto y fracciones de Bursera tomentosa, y su efecto en la viabilidad celular sobre células SiHa.Trabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85EspecializadaORIGINAL2023_SebastianCamiloOrtiz2023_SebastianCamiloOrtizapplication/pdf531047https://repositorio.uan.edu.co/bitstreams/7cedcf7f-b2c2-41c5-9497-c1df1361acf4/downloadea5c63cca4e4f87a1b3298ad0515898dMD512023_SebastianCamiloOrtiz_Autorización2023_SebastianCamiloOrtiz_Autorizaciónapplication/pdf542439https://repositorio.uan.edu.co/bitstreams/467c3adf-1a00-4a26-94b3-462560ce3dda/downloadb9077df775b7db1ebc58a3958abca155MD542023_SebastianCamiloOrtiz_Acta2023_SebastianCamiloOrtiz_Actaapplication/pdf1411772https://repositorio.uan.edu.co/bitstreams/3098af45-3955-4293-ae0e-080ba0740d50/download82f4a359cee8f38ef28445b4bb5feff6MD52CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8811https://repositorio.uan.edu.co/bitstreams/6618c55b-b0c0-445d-b992-14ab573818bd/download9868ccc48a14c8d591352b6eaf7f6239MD55123456789/8799oai:repositorio.uan.edu.co:123456789/87992024-10-09 23:00:48.987https://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertoopen.accesshttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co

Determinación del contenido de polifenoles del extracto y fracciones de Bursera tomentosa, y su efecto en la viabilidad celular sobre células SiHa.

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