Evaluación in vitro de la citotoxicidad de extractos vegetales de Marcetia taxifolia en cultivos celulares para inhibir la proliferación del virus del Zika

Externa

Autores:: León Colmenares, Denis Sofía

Tipo de recurso:: Trabajo de grado de pregrado

Fecha de publicación:: 2020

Institución:: Universidad Antonio Nariño

Repositorio:: Repositorio UAN

Idioma:: spa

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repository_id_str
dc.title.es_ES.fl_str_mv	Evaluación in vitro de la citotoxicidad de extractos vegetales de Marcetia taxifolia en cultivos celulares para inhibir la proliferación del virus del Zika
title	Evaluación in vitro de la citotoxicidad de extractos vegetales de Marcetia taxifolia en cultivos celulares para inhibir la proliferación del virus del Zika
spellingShingle	Evaluación in vitro de la citotoxicidad de extractos vegetales de Marcetia taxifolia en cultivos celulares para inhibir la proliferación del virus del Zika Zika, Marcetia taxifolia, citotoxicidad, susceptibilidad viral Zika, Marcetia taxifolia, cytotoxicity, viral susceptibility
title_short	Evaluación in vitro de la citotoxicidad de extractos vegetales de Marcetia taxifolia en cultivos celulares para inhibir la proliferación del virus del Zika
title_full	Evaluación in vitro de la citotoxicidad de extractos vegetales de Marcetia taxifolia en cultivos celulares para inhibir la proliferación del virus del Zika
title_fullStr	Evaluación in vitro de la citotoxicidad de extractos vegetales de Marcetia taxifolia en cultivos celulares para inhibir la proliferación del virus del Zika
title_full_unstemmed	Evaluación in vitro de la citotoxicidad de extractos vegetales de Marcetia taxifolia en cultivos celulares para inhibir la proliferación del virus del Zika
title_sort	Evaluación in vitro de la citotoxicidad de extractos vegetales de Marcetia taxifolia en cultivos celulares para inhibir la proliferación del virus del Zika
dc.creator.fl_str_mv	León Colmenares, Denis Sofía
dc.contributor.advisor.spa.fl_str_mv	Rodríguez Fersaca, Anny Karely Losada Barragán, Monica Bello García, Felio Jesús Torres García, Orlando Alfredo Muñoz Ramírez, Ana Luisa
dc.contributor.author.spa.fl_str_mv	León Colmenares, Denis Sofía
dc.subject.es_ES.fl_str_mv	Zika, Marcetia taxifolia, citotoxicidad, susceptibilidad viral
topic	Zika, Marcetia taxifolia, citotoxicidad, susceptibilidad viral Zika, Marcetia taxifolia, cytotoxicity, viral susceptibility
dc.subject.keyword.es_ES.fl_str_mv	Zika, Marcetia taxifolia, cytotoxicity, viral susceptibility
description	Externa
publishDate	2020
dc.date.issued.spa.fl_str_mv	2020-11-19
dc.date.accessioned.none.fl_str_mv	2021-02-18T18:45:58Z
dc.date.available.none.fl_str_mv	2021-02-18T18:45:58Z
dc.type.spa.fl_str_mv	Trabajo de grado (Pregrado y/o Especialización)
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dc.identifier.uri.none.fl_str_mv	http://repositorio.uan.edu.co/handle/123456789/1497
dc.identifier.bibliographicCitation.spa.fl_str_mv	Arbovirus en Latinoamérica [Internet]. [cited 2020 Nov 10]. Available from: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0186-23912016000200111 DE COMBATE AL ZIKA SISTEMATIZACIÓN DE LA ESTRATEGIA [Internet]. [cited 2019 Oct 24]. Available from: http://www.paho.org/hq/index.php?option=com_content&view=article&id=12861%3A2016-zika-evolved-from-emerge Vector competence: Entomological considerations and its implications on the epidemiology of Dengue [Internet]. [cited 2020 May 23]. Available from: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121-07932010000200006 De F, De C, Salud LA. Universidad Privada Norbert Wiener. 2018; Available from: http://repositorio.uwiener.edu.pe/bitstream/handle/123456789/956/ESPECIALIDAD - Montero Crisologo%2C Diana Carolina.pdf?sequence=4&isAllowed=y Krow-Lucal ER, Biggerstaff BJ, Staples JE. Estimated Incubation Period for Zika Virus Disease. Emerg Infect Dis [Internet]. 2017 [cited 2019 Oct 24];23(5):841–5. Available from: http://www.ncbi.nlm.nih.gov/pubmed/28277198 Cabezas C, García P. Diagnóstico de la infección por el virus zika Diagnosis of Zika virus infection. [cited 2019 Oct 24]; Available from: http://dx.doi.org/10.15381/anales.v78i1.13028 Carod-Artal FJ. Neurological complications associated with dengue virus infection. Rev Neurol [Internet]. 2019 [cited 2020 Nov 10];69(3):113–22. Available from: https://www.neurologia.com/articulo/2019140 Síndrome de Guillain–Barré [Internet]. [cited 2020 Oct 18]. Available from: https://www.who.int/es/news-room/fact-sheets/detail/guillain-barré-syndrome (No Title) [Internet]. [cited 2020 Nov 11]. Available from: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/ED/VSP/Zica-Mayo-2015-Analisis-Riesgo.pdf Cao-Lormeau V-M, Blake A, Mons S, Lastère S, Roche C, Vanhomwegen J, et al. Guillain-Barré Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study. Lancet (London, England) [Internet]. 2016 Apr 9 [cited 2019 Oct 24];387(10027):1531–9. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26948433 Faheem M, Naseer MI, Rasool M, Chaudhary AG, Kumosani TA, Ilyas AM, et al. Molecular genetics of human primary microcephaly: an overview. BMC Med Genomics [Internet]. 2015 [cited 2019 Oct 24];8 Suppl 1:S4. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25951892 Ministerio de Salud y Protección Social [Internet]. [cited 2019 Oct 24]. Available from: https://www.minsalud.gov.co/Paginas/default.aspx Semanal BE. Semana epidemiológica 18 26 de abril al 2 de mayo de 2020. (No Title) [Internet]. [cited 2020 Nov 11]. Available from: http://www.scielo.org.co/pdf/cesm/v33n1/0120-8705-cesm-33-01-42.pdf Betancur-Galvis LA, Saez J, Granados H, Salazar A, Ossa JE. Antitumor and Antiviral Activity of Colombian Medicinal Plant Extracts. Vol. 94, Mem Inst Oswaldo Cruz, Rio de Janeiro. Ortega JT, Serrano ML, Suárez AI, Baptista J, Pujol FH, Rangel HR. Methoxyflavones from Marcetia taxifolia as HIV-1 Reverse Transcriptase Inhibitors. Scheck AC, Perry K, Hank NC, Clark WD. Anticancer activity of extracts derived from the mature roots of Scutellaria baicalensis on human malignant brain tumor cells. BMC Complement Altern Med. 2006 Aug 16;6. Chiow KH, Phoon MC, Putti T, Tan BKH, Chow VT. Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection. Vol. 9, Asian Pacific Journal of Tropical Medicine. Elsevier (Singapore) Pte Ltd; 2016. p. 1–7. Chen H, Yang C, Ke T, Zhou M, Li Z, Zhang M, et al. Antimicrobial activity of secondary metabolites from Streptomyces sp. K15, an endophyte in Houttuynia cordata Thunb. Nat Prod Res. 2015 Dec 2;29(23):2223–5. Carneiro BM, Batista MN, Braga ACS, Nogueira ML, Rahal P. The green tea molecule EGCG inhibits Zika virus entry. Virology. 2016 Sep 1;496:215–8. Esteban QUISPE BRAVO ASESOR B. Actividad inhibitoria de los aceites esenciales de Lippia alba contra el virus zika en células VERO – 76 y C6/36 de Aedes albopictus. Universidad Nacional Mayor de San Marcos. Universidad Nacional Mayor de San Marcos; 2018. Singh P, Chakraborty P, He D-H, Mergia A. Extract prepared from the leaves of Ocimum basilicum inhibits the entry of Zika virus. Kistner O, Barrett PN, Mundt W, Reiter M, Schober-Bendixen S, Dorner F. Development of a mammalian cell (Vero) derived candidate influenza virus vaccine. Vaccine [Internet]. 1998 May [cited 2020 Nov 11];16(9–10):960–8. Available from: https://pubmed.ncbi.nlm.nih.gov/9682344/ Govorkova EA, Murti G, Meignier B, de Taisne C, Webster RG. African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses. J Virol [Internet]. 1996 Aug 1 [cited 2020 Nov 11];70(8):5519–24. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/8764064/?tool=EBI Fabiola López de la Torre M, Ana Denisse Re Araujo D, Avalos Borja Alexei Fedorovish Licea Navarro M, Negrete Regagnon P, Rufina Hernández Martínez David Hilario Covarrubias Rosales D. i TESIS DEFENDIDA POR. Jorquera A. Tratamiento. Enfermedad de Chagas. Biomédica. 2011;31(sup3.2):403 Mosmann T. Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. Vol. 65, Journal oflmmunologicalMethods. 1983. Evaluación de la citotoxicidad de extractos marinos sobre un panel de líneas celulares \| Díaz-García \| Revista Cubana de Farmacia [Internet]. [cited 2020 Oct 16]. Available from: http://revfarmacia.sld.cu/index.php/far/article/view/12/13 Baptista J, Chávez K, Torrico F, Trejo E, Garcia CC, Urbina J, et al. Constituyentes químicos y actividad antiinflamatoria de Marcetia taxifolia. Vol. 24, Scientific Journal from the Experimental Faculty of Sciences, at the Universidad del Zulia. Abril-Junio; 2016. Dorantes-Álvarez L, Salas-Benito J, Barrón-Romero B, Salas-Benito M, De Nova-Ocampo M. CITOTOXICIDAD Y ACTIVIDAD ANTIVIRAL DE EXTRACTOS DE CHILES (Capsicum spp) CYTOTOXICITY AND ANTIVIRAL ACTIVITY OF PEPPER EXTRACTS (Capsicum spp). Inst Politécnico Nac Núm [Internet]. 2018 [cited 2020 Oct 16];46:273–85. Available from: http:www.polibotanica.mx (No Title) [Internet]. [cited 2020 Oct 15]. Available from: https://www.formacionegs.com/archivos/1325673989.pdf Restrepo-Mendez LC, Zapata-Cardona MI, Gutierrez MM. Evaluación in vitro del efecto virucida e interacciones in sílico del Lupeol contra los cuatro serotipos del virus Dengue AUTORES. Actividad citotóxica in vitro en líneas celulares y células de sangre periférica humana de los alcaloides totales de corteza de Galipea longiflora (Evanta) [Internet]. [cited 2020 Nov 18]. Available from: http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S0250-54602009000200008 Ocazionez RE, Meneses R, Torres FÁ, Stashenko E. Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro. Mem Inst Oswaldo Cruz [Internet]. 2010 [cited 2020 Nov 18];105(3):304–9. Available from: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300010&lng=en&nrm=iso&tlng=en Sánchez I, Gómez-Garibay F, Taboada J, Ruiz BH. Antiviral effect of flavonoids on the dengue virus. Phyther Res [Internet]. 2000 Mar 1 [cited 2020 Nov 18];14(2):89–92. Available from: https://onlinelibrary.wiley.com/doi/full/10.1002/%28SICI%291099-1573%28200003%2914%3A2%3C89%3A%3AAID-PTR569%3E3.0.CO%3B2-C Andrighetti-Fröhner CR, Sincero TCM, Da Silva AC, Savi LA, Gaido CM, Bettega JMR, et al. Antiviral evaluation of plants from Brazilian Atlantic Tropical Forest. Fitoterapia. 2005 Jun 1;76(3–4):374–8.
dc.identifier.instname.spa.fl_str_mv	instname:Universidad Antonio Nariño
dc.identifier.reponame.spa.fl_str_mv	reponame:Repositorio Institucional UAN
dc.identifier.repourl.spa.fl_str_mv	repourl:https://repositorio.uan.edu.co/
url	http://repositorio.uan.edu.co/handle/123456789/1497
identifier_str_mv	Arbovirus en Latinoamérica [Internet]. [cited 2020 Nov 10]. Available from: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0186-23912016000200111 DE COMBATE AL ZIKA SISTEMATIZACIÓN DE LA ESTRATEGIA [Internet]. [cited 2019 Oct 24]. Available from: http://www.paho.org/hq/index.php?option=com_content&view=article&id=12861%3A2016-zika-evolved-from-emerge Vector competence: Entomological considerations and its implications on the epidemiology of Dengue [Internet]. [cited 2020 May 23]. Available from: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121-07932010000200006 De F, De C, Salud LA. Universidad Privada Norbert Wiener. 2018; Available from: http://repositorio.uwiener.edu.pe/bitstream/handle/123456789/956/ESPECIALIDAD - Montero Crisologo%2C Diana Carolina.pdf?sequence=4&isAllowed=y Krow-Lucal ER, Biggerstaff BJ, Staples JE. Estimated Incubation Period for Zika Virus Disease. Emerg Infect Dis [Internet]. 2017 [cited 2019 Oct 24];23(5):841–5. Available from: http://www.ncbi.nlm.nih.gov/pubmed/28277198 Cabezas C, García P. Diagnóstico de la infección por el virus zika Diagnosis of Zika virus infection. [cited 2019 Oct 24]; Available from: http://dx.doi.org/10.15381/anales.v78i1.13028 Carod-Artal FJ. Neurological complications associated with dengue virus infection. Rev Neurol [Internet]. 2019 [cited 2020 Nov 10];69(3):113–22. Available from: https://www.neurologia.com/articulo/2019140 Síndrome de Guillain–Barré [Internet]. [cited 2020 Oct 18]. Available from: https://www.who.int/es/news-room/fact-sheets/detail/guillain-barré-syndrome (No Title) [Internet]. [cited 2020 Nov 11]. Available from: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/ED/VSP/Zica-Mayo-2015-Analisis-Riesgo.pdf Cao-Lormeau V-M, Blake A, Mons S, Lastère S, Roche C, Vanhomwegen J, et al. Guillain-Barré Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study. Lancet (London, England) [Internet]. 2016 Apr 9 [cited 2019 Oct 24];387(10027):1531–9. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26948433 Faheem M, Naseer MI, Rasool M, Chaudhary AG, Kumosani TA, Ilyas AM, et al. Molecular genetics of human primary microcephaly: an overview. BMC Med Genomics [Internet]. 2015 [cited 2019 Oct 24];8 Suppl 1:S4. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25951892 Ministerio de Salud y Protección Social [Internet]. [cited 2019 Oct 24]. Available from: https://www.minsalud.gov.co/Paginas/default.aspx Semanal BE. Semana epidemiológica 18 26 de abril al 2 de mayo de 2020. (No Title) [Internet]. [cited 2020 Nov 11]. Available from: http://www.scielo.org.co/pdf/cesm/v33n1/0120-8705-cesm-33-01-42.pdf Betancur-Galvis LA, Saez J, Granados H, Salazar A, Ossa JE. Antitumor and Antiviral Activity of Colombian Medicinal Plant Extracts. Vol. 94, Mem Inst Oswaldo Cruz, Rio de Janeiro. Ortega JT, Serrano ML, Suárez AI, Baptista J, Pujol FH, Rangel HR. Methoxyflavones from Marcetia taxifolia as HIV-1 Reverse Transcriptase Inhibitors. Scheck AC, Perry K, Hank NC, Clark WD. Anticancer activity of extracts derived from the mature roots of Scutellaria baicalensis on human malignant brain tumor cells. BMC Complement Altern Med. 2006 Aug 16;6. Chiow KH, Phoon MC, Putti T, Tan BKH, Chow VT. Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection. Vol. 9, Asian Pacific Journal of Tropical Medicine. Elsevier (Singapore) Pte Ltd; 2016. p. 1–7. Chen H, Yang C, Ke T, Zhou M, Li Z, Zhang M, et al. Antimicrobial activity of secondary metabolites from Streptomyces sp. K15, an endophyte in Houttuynia cordata Thunb. Nat Prod Res. 2015 Dec 2;29(23):2223–5. Carneiro BM, Batista MN, Braga ACS, Nogueira ML, Rahal P. The green tea molecule EGCG inhibits Zika virus entry. Virology. 2016 Sep 1;496:215–8. Esteban QUISPE BRAVO ASESOR B. Actividad inhibitoria de los aceites esenciales de Lippia alba contra el virus zika en células VERO – 76 y C6/36 de Aedes albopictus. Universidad Nacional Mayor de San Marcos. Universidad Nacional Mayor de San Marcos; 2018. Singh P, Chakraborty P, He D-H, Mergia A. Extract prepared from the leaves of Ocimum basilicum inhibits the entry of Zika virus. Kistner O, Barrett PN, Mundt W, Reiter M, Schober-Bendixen S, Dorner F. Development of a mammalian cell (Vero) derived candidate influenza virus vaccine. Vaccine [Internet]. 1998 May [cited 2020 Nov 11];16(9–10):960–8. Available from: https://pubmed.ncbi.nlm.nih.gov/9682344/ Govorkova EA, Murti G, Meignier B, de Taisne C, Webster RG. African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses. J Virol [Internet]. 1996 Aug 1 [cited 2020 Nov 11];70(8):5519–24. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/8764064/?tool=EBI Fabiola López de la Torre M, Ana Denisse Re Araujo D, Avalos Borja Alexei Fedorovish Licea Navarro M, Negrete Regagnon P, Rufina Hernández Martínez David Hilario Covarrubias Rosales D. i TESIS DEFENDIDA POR. Jorquera A. Tratamiento. Enfermedad de Chagas. Biomédica. 2011;31(sup3.2):403 Mosmann T. Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. Vol. 65, Journal oflmmunologicalMethods. 1983. Evaluación de la citotoxicidad de extractos marinos sobre un panel de líneas celulares \| Díaz-García \| Revista Cubana de Farmacia [Internet]. [cited 2020 Oct 16]. Available from: http://revfarmacia.sld.cu/index.php/far/article/view/12/13 Baptista J, Chávez K, Torrico F, Trejo E, Garcia CC, Urbina J, et al. Constituyentes químicos y actividad antiinflamatoria de Marcetia taxifolia. Vol. 24, Scientific Journal from the Experimental Faculty of Sciences, at the Universidad del Zulia. Abril-Junio; 2016. Dorantes-Álvarez L, Salas-Benito J, Barrón-Romero B, Salas-Benito M, De Nova-Ocampo M. CITOTOXICIDAD Y ACTIVIDAD ANTIVIRAL DE EXTRACTOS DE CHILES (Capsicum spp) CYTOTOXICITY AND ANTIVIRAL ACTIVITY OF PEPPER EXTRACTS (Capsicum spp). Inst Politécnico Nac Núm [Internet]. 2018 [cited 2020 Oct 16];46:273–85. Available from: http:www.polibotanica.mx (No Title) [Internet]. [cited 2020 Oct 15]. Available from: https://www.formacionegs.com/archivos/1325673989.pdf Restrepo-Mendez LC, Zapata-Cardona MI, Gutierrez MM. Evaluación in vitro del efecto virucida e interacciones in sílico del Lupeol contra los cuatro serotipos del virus Dengue AUTORES. Actividad citotóxica in vitro en líneas celulares y células de sangre periférica humana de los alcaloides totales de corteza de Galipea longiflora (Evanta) [Internet]. [cited 2020 Nov 18]. Available from: http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S0250-54602009000200008 Ocazionez RE, Meneses R, Torres FÁ, Stashenko E. Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro. Mem Inst Oswaldo Cruz [Internet]. 2010 [cited 2020 Nov 18];105(3):304–9. Available from: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300010&lng=en&nrm=iso&tlng=en Sánchez I, Gómez-Garibay F, Taboada J, Ruiz BH. Antiviral effect of flavonoids on the dengue virus. Phyther Res [Internet]. 2000 Mar 1 [cited 2020 Nov 18];14(2):89–92. Available from: https://onlinelibrary.wiley.com/doi/full/10.1002/%28SICI%291099-1573%28200003%2914%3A2%3C89%3A%3AAID-PTR569%3E3.0.CO%3B2-C Andrighetti-Fröhner CR, Sincero TCM, Da Silva AC, Savi LA, Gaido CM, Bettega JMR, et al. Antiviral evaluation of plants from Brazilian Atlantic Tropical Forest. Fitoterapia. 2005 Jun 1;76(3–4):374–8. instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/
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spelling	Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2Rodríguez Fersaca, Anny KarelyLosada Barragán, MonicaBello García, Felio JesúsTorres García, Orlando AlfredoMuñoz Ramírez, Ana LuisaLeón Colmenares, Denis Sofía10239701792021-02-18T18:45:58Z2021-02-18T18:45:58Z2020-11-19http://repositorio.uan.edu.co/handle/123456789/1497Arbovirus en Latinoamérica [Internet]. [cited 2020 Nov 10]. Available from: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0186-23912016000200111DE COMBATE AL ZIKA SISTEMATIZACIÓN DE LA ESTRATEGIA [Internet]. [cited 2019 Oct 24]. Available from: http://www.paho.org/hq/index.php?option=com_content&view=article&id=12861%3A2016-zika-evolved-from-emergeVector competence: Entomological considerations and its implications on the epidemiology of Dengue [Internet]. [cited 2020 May 23]. Available from: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0121-07932010000200006De F, De C, Salud LA. Universidad Privada Norbert Wiener. 2018; Available from: http://repositorio.uwiener.edu.pe/bitstream/handle/123456789/956/ESPECIALIDAD - Montero Crisologo%2C Diana Carolina.pdf?sequence=4&isAllowed=yKrow-Lucal ER, Biggerstaff BJ, Staples JE. Estimated Incubation Period for Zika Virus Disease. Emerg Infect Dis [Internet]. 2017 [cited 2019 Oct 24];23(5):841–5. Available from: http://www.ncbi.nlm.nih.gov/pubmed/28277198Cabezas C, García P. Diagnóstico de la infección por el virus zika Diagnosis of Zika virus infection. [cited 2019 Oct 24]; Available from: http://dx.doi.org/10.15381/anales.v78i1.13028Carod-Artal FJ. Neurological complications associated with dengue virus infection. Rev Neurol [Internet]. 2019 [cited 2020 Nov 10];69(3):113–22. Available from: https://www.neurologia.com/articulo/2019140Síndrome de Guillain–Barré [Internet]. [cited 2020 Oct 18]. Available from: https://www.who.int/es/news-room/fact-sheets/detail/guillain-barré-syndrome(No Title) [Internet]. [cited 2020 Nov 11]. Available from: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/VS/ED/VSP/Zica-Mayo-2015-Analisis-Riesgo.pdfCao-Lormeau V-M, Blake A, Mons S, Lastère S, Roche C, Vanhomwegen J, et al. Guillain-Barré Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study. Lancet (London, England) [Internet]. 2016 Apr 9 [cited 2019 Oct 24];387(10027):1531–9. Available from: http://www.ncbi.nlm.nih.gov/pubmed/26948433Faheem M, Naseer MI, Rasool M, Chaudhary AG, Kumosani TA, Ilyas AM, et al. Molecular genetics of human primary microcephaly: an overview. BMC Med Genomics [Internet]. 2015 [cited 2019 Oct 24];8 Suppl 1:S4. Available from: http://www.ncbi.nlm.nih.gov/pubmed/25951892Ministerio de Salud y Protección Social [Internet]. [cited 2019 Oct 24]. Available from: https://www.minsalud.gov.co/Paginas/default.aspxSemanal BE. Semana epidemiológica 18 26 de abril al 2 de mayo de 2020.(No Title) [Internet]. [cited 2020 Nov 11]. Available from: http://www.scielo.org.co/pdf/cesm/v33n1/0120-8705-cesm-33-01-42.pdfBetancur-Galvis LA, Saez J, Granados H, Salazar A, Ossa JE. Antitumor and Antiviral Activity of Colombian Medicinal Plant Extracts. Vol. 94, Mem Inst Oswaldo Cruz, Rio de Janeiro.Ortega JT, Serrano ML, Suárez AI, Baptista J, Pujol FH, Rangel HR. Methoxyflavones from Marcetia taxifolia as HIV-1 Reverse Transcriptase Inhibitors.Scheck AC, Perry K, Hank NC, Clark WD. Anticancer activity of extracts derived from the mature roots of Scutellaria baicalensis on human malignant brain tumor cells. BMC Complement Altern Med. 2006 Aug 16;6.Chiow KH, Phoon MC, Putti T, Tan BKH, Chow VT. Evaluation of antiviral activities of Houttuynia cordata Thunb. extract, quercetin, quercetrin and cinanserin on murine coronavirus and dengue virus infection. Vol. 9, Asian Pacific Journal of Tropical Medicine. Elsevier (Singapore) Pte Ltd; 2016. p. 1–7.Chen H, Yang C, Ke T, Zhou M, Li Z, Zhang M, et al. Antimicrobial activity of secondary metabolites from Streptomyces sp. K15, an endophyte in Houttuynia cordata Thunb. Nat Prod Res. 2015 Dec 2;29(23):2223–5.Carneiro BM, Batista MN, Braga ACS, Nogueira ML, Rahal P. The green tea molecule EGCG inhibits Zika virus entry. Virology. 2016 Sep 1;496:215–8.Esteban QUISPE BRAVO ASESOR B. Actividad inhibitoria de los aceites esenciales de Lippia alba contra el virus zika en células VERO – 76 y C6/36 de Aedes albopictus. Universidad Nacional Mayor de San Marcos. Universidad Nacional Mayor de San Marcos; 2018.Singh P, Chakraborty P, He D-H, Mergia A. Extract prepared from the leaves of Ocimum basilicum inhibits the entry of Zika virus.Kistner O, Barrett PN, Mundt W, Reiter M, Schober-Bendixen S, Dorner F. Development of a mammalian cell (Vero) derived candidate influenza virus vaccine. Vaccine [Internet]. 1998 May [cited 2020 Nov 11];16(9–10):960–8. Available from: https://pubmed.ncbi.nlm.nih.gov/9682344/Govorkova EA, Murti G, Meignier B, de Taisne C, Webster RG. African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses. J Virol [Internet]. 1996 Aug 1 [cited 2020 Nov 11];70(8):5519–24. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/8764064/?tool=EBIFabiola López de la Torre M, Ana Denisse Re Araujo D, Avalos Borja Alexei Fedorovish Licea Navarro M, Negrete Regagnon P, Rufina Hernández Martínez David Hilario Covarrubias Rosales D. i TESIS DEFENDIDA POR.Jorquera A. Tratamiento. Enfermedad de Chagas. Biomédica. 2011;31(sup3.2):403Mosmann T. Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. Vol. 65, Journal oflmmunologicalMethods. 1983.Evaluación de la citotoxicidad de extractos marinos sobre un panel de líneas celulares \| Díaz-García \| Revista Cubana de Farmacia [Internet]. [cited 2020 Oct 16]. Available from: http://revfarmacia.sld.cu/index.php/far/article/view/12/13Baptista J, Chávez K, Torrico F, Trejo E, Garcia CC, Urbina J, et al. Constituyentes químicos y actividad antiinflamatoria de Marcetia taxifolia. Vol. 24, Scientific Journal from the Experimental Faculty of Sciences, at the Universidad del Zulia. Abril-Junio; 2016.Dorantes-Álvarez L, Salas-Benito J, Barrón-Romero B, Salas-Benito M, De Nova-Ocampo M. CITOTOXICIDAD Y ACTIVIDAD ANTIVIRAL DE EXTRACTOS DE CHILES (Capsicum spp) CYTOTOXICITY AND ANTIVIRAL ACTIVITY OF PEPPER EXTRACTS (Capsicum spp). Inst Politécnico Nac Núm [Internet]. 2018 [cited 2020 Oct 16];46:273–85. Available from: http:www.polibotanica.mx(No Title) [Internet]. [cited 2020 Oct 15]. Available from: https://www.formacionegs.com/archivos/1325673989.pdfRestrepo-Mendez LC, Zapata-Cardona MI, Gutierrez MM. Evaluación in vitro del efecto virucida e interacciones in sílico del Lupeol contra los cuatro serotipos del virus Dengue AUTORES.Actividad citotóxica in vitro en líneas celulares y células de sangre periférica humana de los alcaloides totales de corteza de Galipea longiflora (Evanta) [Internet]. [cited 2020 Nov 18]. Available from: http://www.scielo.org.bo/scielo.php?script=sci_arttext&pid=S0250-54602009000200008Ocazionez RE, Meneses R, Torres FÁ, Stashenko E. Virucidal activity of Colombian Lippia essential oils on dengue virus replication in vitro. Mem Inst Oswaldo Cruz [Internet]. 2010 [cited 2020 Nov 18];105(3):304–9. Available from: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000300010&lng=en&nrm=iso&tlng=enSánchez I, Gómez-Garibay F, Taboada J, Ruiz BH. Antiviral effect of flavonoids on the dengue virus. Phyther Res [Internet]. 2000 Mar 1 [cited 2020 Nov 18];14(2):89–92. Available from: https://onlinelibrary.wiley.com/doi/full/10.1002/%28SICI%291099-1573%28200003%2914%3A2%3C89%3A%3AAID-PTR569%3E3.0.CO%3B2-CAndrighetti-Fröhner CR, Sincero TCM, Da Silva AC, Savi LA, Gaido CM, Bettega JMR, et al. Antiviral evaluation of plants from Brazilian Atlantic Tropical Forest. Fitoterapia. 2005 Jun 1;76(3–4):374–8.instname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/ExternaZika virus infection is a recurrent problem worldwide, affecting pregnant women and children, to the elderly, leading to complications, which could even be lethal, such as microcephaly observed in fetuses and babies infected by the Zika virus. virus during the gestation stage. Additionally, other complications observed are neurological manifestations, such as Guillain-Barré syndrome, which can appear during the acute phase of infection or after it. The syndrome affects the nerves that control muscle movement and the nerves that transmit pain, heat, and touch. This can lead to muscle weakness and loss of sensation in the legs or arms, and even in the worst case it can lead to death. Although people of all ages can be affected, it is more common in adults and men. Despite this, there is no specific antiviral treatment for this infection, therefore, the main objective of this project was to evaluate the cytotoxicity of plants extracts of Marcetia taxifolia against cell cultures derived from mammals, for a subsequent study of these against the Zika virus. For this, a series of dilutions was made to each of the Marcetia taxifolia extracts, in different concentrations (1, 10, 50, 100, 200 and 300 ug / mL) they were put in interaction with two different cell lines (BHK-21 and Vero) for the subsequent determination of its cytotoxicity. With these results, it is expected later to do viral inhibition tests. In addition, it is expected to demonstrate that it is possible to include the use of bioactive compounds extracted from Marcetia taxifolia as potential anti-infective products, which can be an effective, biosustainable and efficient method in the control of this type of infection.La infección por el virus del Zika es una problemática reincidente a nivel mundial, afectando desde mujeres embarazadas y niños, hasta ancianos, conllevando a complicaciones, que podrían incluso ser letales, como lo son la microcefalia que se observa en fetos y bebés infectados por el virus durante la etapa de gestación. Adicionalmente, otras complicaciones observadas son las manifestaciones neurológicas, como el síndrome de Guillain-Barré, que pueden aparecer durante la fase aguda de la infección o después de ella. El síndrome afecta los nervios que controlan el movimiento de los músculos y los nervios que transmiten el dolor, el calor y el tacto. Esto puede provocar debilidad muscular y pérdida de sensibilidad en piernas o brazos, e incluso en el peor de los casos puede causar la muerte. Aunque las personas de todas las edades pueden verse afectadas, es más común que se dé entre adultos y hombres. A pesar de ello, no existe un tratamiento antiviral específico para esta infección, por lo tanto, el objetivo principal de este proyecto fue evaluar la citotoxicidad de extractos naturales de Marcetia taxifolia frente a cultivos celulares derivados de mamíferos, para un posterior estudio de éstos frente al virus del Zika. Para ello se realizó una serie de diluciones a cada uno de los extractos de Marcetia taxifolia, en diferentes concentraciones (1, 10, 50, 100, 200 y 300 ug/mL) fueron puestas en interacción con dos líneas celulares diferentes (BHK-21 y Vero) para la posterior determinación de su citotoxicidad. Con estos resultados, se espera más adelante hacer ensayos de inhibición viral. Además, se espera demostrar que es posible incluir el uso de compuestos bioactivos extraídos de Marcetia taxifolia como potenciales productos antiinfecciosos, pudiendo ser un método eficaz, biosostenible y eficiente en el control de este tipo de infección.Programa Nacional en Ciencias BásicasBioquímico(a)PregradoPresencialspaUniversidad Antonio NariñoBioquímicaFacultad de CienciasBogotá - CircunvalarZika, Marcetia taxifolia, citotoxicidad, susceptibilidad viralZika, Marcetia taxifolia, cytotoxicity, viral susceptibilityEvaluación in vitro de la citotoxicidad de extractos vegetales de Marcetia taxifolia en cultivos celulares para inhibir la proliferación del virus del ZikaTrabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85ORIGINAL2020DenisSofiaLeonColmenares.pdf2020DenisSofiaLeonColmenares.pdfTrabajo de gradoapplication/pdf1354155https://repositorio.uan.edu.co/bitstreams/5e4a6d8d-c248-4959-be7d-56b8678f881a/download217b6cb6f1e0246e08834739dba90d87MD512020AutorizacióndeAutores.pdf2020AutorizacióndeAutores.pdfAutorización autoresapplication/pdf555857https://repositorio.uan.edu.co/bitstreams/2b02a5dd-141a-4b16-96c3-da691eb5938f/download50be141c47515eb48fb1f224686ae0b8MD53CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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Evaluación in vitro de la citotoxicidad de extractos vegetales de Marcetia taxifolia en cultivos celulares para inhibir la proliferación del virus del Zika

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