Identificación de Marcadores TRA-l-60, OCT-4, NANOG y S SEA-4 en Muestras de Células Madre Cancerosas de Pacientes Diagnosticados con Cáncer Oral
Introduction: Oral cancer can result from a primary lesion or originate from another distant or adjacent tumor lesion leading to malignant neoplasms in the structures and tissues of the oral cavity (metastasis). Objective: To identify TRA-I-60, OCT-4, NANOG and S SEA-4 markers in cancer stem cells o...
- Autores:
-
Bustos Pérez, Diego Andrés
Trujillo Reyes, Karen Daniela
- Tipo de recurso:
- Trabajo de grado de pregrado
- Fecha de publicación:
- 2022
- Institución:
- Universidad Antonio Nariño
- Repositorio:
- Repositorio UAN
- Idioma:
- spa
- OAI Identifier:
- oai:repositorio.uan.edu.co:123456789/6977
- Acceso en línea:
- http://repositorio.uan.edu.co/handle/123456789/6977
- Palabra clave:
- Carcinoma Escamocelular
TRA-l-60
OCT-4
TRA
NANOG
S SEA-4
Squamous cell carcinoma
TRA-l-60,
OCT-4
TRA
NANOG and S SEA-4.
- Rights
- openAccess
- License
- Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
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| dc.title.es_ES.fl_str_mv |
Identificación de Marcadores TRA-l-60, OCT-4, NANOG y S SEA-4 en Muestras de Células Madre Cancerosas de Pacientes Diagnosticados con Cáncer Oral |
| title |
Identificación de Marcadores TRA-l-60, OCT-4, NANOG y S SEA-4 en Muestras de Células Madre Cancerosas de Pacientes Diagnosticados con Cáncer Oral |
| spellingShingle |
Identificación de Marcadores TRA-l-60, OCT-4, NANOG y S SEA-4 en Muestras de Células Madre Cancerosas de Pacientes Diagnosticados con Cáncer Oral Carcinoma Escamocelular TRA-l-60 OCT-4 TRA NANOG S SEA-4 Squamous cell carcinoma TRA-l-60, OCT-4 TRA NANOG and S SEA-4. |
| title_short |
Identificación de Marcadores TRA-l-60, OCT-4, NANOG y S SEA-4 en Muestras de Células Madre Cancerosas de Pacientes Diagnosticados con Cáncer Oral |
| title_full |
Identificación de Marcadores TRA-l-60, OCT-4, NANOG y S SEA-4 en Muestras de Células Madre Cancerosas de Pacientes Diagnosticados con Cáncer Oral |
| title_fullStr |
Identificación de Marcadores TRA-l-60, OCT-4, NANOG y S SEA-4 en Muestras de Células Madre Cancerosas de Pacientes Diagnosticados con Cáncer Oral |
| title_full_unstemmed |
Identificación de Marcadores TRA-l-60, OCT-4, NANOG y S SEA-4 en Muestras de Células Madre Cancerosas de Pacientes Diagnosticados con Cáncer Oral |
| title_sort |
Identificación de Marcadores TRA-l-60, OCT-4, NANOG y S SEA-4 en Muestras de Células Madre Cancerosas de Pacientes Diagnosticados con Cáncer Oral |
| dc.creator.fl_str_mv |
Bustos Pérez, Diego Andrés Trujillo Reyes, Karen Daniela |
| dc.contributor.advisor.spa.fl_str_mv |
García Florez, Manuel García Rojas, Claudia Lorena |
| dc.contributor.author.spa.fl_str_mv |
Bustos Pérez, Diego Andrés Trujillo Reyes, Karen Daniela |
| dc.subject.es_ES.fl_str_mv |
Carcinoma Escamocelular TRA-l-60 OCT-4 TRA NANOG S SEA-4 |
| topic |
Carcinoma Escamocelular TRA-l-60 OCT-4 TRA NANOG S SEA-4 Squamous cell carcinoma TRA-l-60, OCT-4 TRA NANOG and S SEA-4. |
| dc.subject.keyword.es_ES.fl_str_mv |
Squamous cell carcinoma TRA-l-60, OCT-4 TRA NANOG and S SEA-4. |
| description |
Introduction: Oral cancer can result from a primary lesion or originate from another distant or adjacent tumor lesion leading to malignant neoplasms in the structures and tissues of the oral cavity (metastasis). Objective: To identify TRA-I-60, OCT-4, NANOG and S SEA-4 markers in cancer stem cells of patients diagnosed with oral cancer. Materials and Methods: It corresponds to an experimental type research, by Immunofluorescence method, 5 μm slices will be routinely deparaffinized and then rehydrated. antigenic recovery will be performed in buffer 10 mm citrate buffer, Ph 6.0 for 15 minutes in an electric pressure cooker. the slides will be incubated in H2O2 1% for 15 minutes, to block endogenous peroxidase. Then 3% BSA will be used for one hour to block nonspecific binding. The sections will be incubated overnight at 4°C with the corresponding primary antibody (antiTral-60, anti-OCT-4, anti-NANOG, anti-S SSE-4), at a 1:100 dilution in 1% BSA. Results: OCT 4 marker manifested with higher intensity in well-differentiated cancer, SSEA4 marker increases with tissue dedifferentiation and NANOG and TRA-1-60 markers remain constant during tumor progression. Conclusions: Of the different markers analyzed related to progenitor cells, all were found in the patient samples, indicating among them more evident marker differences. |
| publishDate |
2022 |
| dc.date.accessioned.none.fl_str_mv |
2022-09-28T23:55:34Z |
| dc.date.available.none.fl_str_mv |
2022-09-28T23:55:34Z |
| dc.date.issued.spa.fl_str_mv |
2022-05-25 |
| dc.type.spa.fl_str_mv |
Trabajo de grado (Pregrado y/o Especialización) |
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http://purl.org/coar/resource_type/c_7a1f |
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http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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http://purl.org/coar/resource_type/c_7a1f |
| dc.identifier.uri.none.fl_str_mv |
http://repositorio.uan.edu.co/handle/123456789/6977 |
| dc.identifier.bibliographicCitation.spa.fl_str_mv |
Acevedo, P. (2015). Stem cells: general aspects, biological and molecular events. Medellin: Scielo. Aghajani, F. (2018). Evaluation of immunophenotyping, proliferation and osteogenic differentiation potential of SSEA-4 positive stem cells derived from pulp of deciduous teeth. Teheran (Iran): pubmed. Alcalá P, D. (2014). Células madre cancerígenas: conceptos actuales. México D.F.: Medigraphic. Badcock, P. (2014). The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. New yor. Fernández, A. (2008). Regeneration of the ocular surface: stem cells and reconstructive techniques. Pamplona (España): scielo. Grubelnik, G., Bostjan, E., & Pavlic, A. (2020). NANOG expression in human development and cancerogenesis. Zagreb (Croacia): Pubmed. Hernán, R., & Dorticós B, E. (2004). Medicina regenerativa. Células madre embrionarias y adultas. Habana (Cuba): Scielo. Herrera B, J. (2013). In vitro Translocation Cytoplasm/nucleus of Embryonic Transcription Factor OCT-4 in Perivascular Cells Suggests that Aorta as a Niche of Quiescent Adult Stem Cells. International Journal of Morphology, 1430-1438. Herrera B, J. (2013). In vitro Translocation Cytoplasm/nucleus of Embryonic Transcription Factor OCT-4 in Perivascular Cells Suggests that Aorta as a Niche of Quiescent Adult Stem Cells. International Journal of Morphology, 1430-1438. HERZUMA. (2020). Programa Científico SEOM. Barcelona (España): HERZUMA |
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instname:Universidad Antonio Nariño |
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reponame:Repositorio Institucional UAN |
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repourl:https://repositorio.uan.edu.co/ |
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http://repositorio.uan.edu.co/handle/123456789/6977 |
| identifier_str_mv |
Acevedo, P. (2015). Stem cells: general aspects, biological and molecular events. Medellin: Scielo. Aghajani, F. (2018). Evaluation of immunophenotyping, proliferation and osteogenic differentiation potential of SSEA-4 positive stem cells derived from pulp of deciduous teeth. Teheran (Iran): pubmed. Alcalá P, D. (2014). Células madre cancerígenas: conceptos actuales. México D.F.: Medigraphic. Badcock, P. (2014). The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. New yor. Fernández, A. (2008). Regeneration of the ocular surface: stem cells and reconstructive techniques. Pamplona (España): scielo. Grubelnik, G., Bostjan, E., & Pavlic, A. (2020). NANOG expression in human development and cancerogenesis. Zagreb (Croacia): Pubmed. Hernán, R., & Dorticós B, E. (2004). Medicina regenerativa. Células madre embrionarias y adultas. Habana (Cuba): Scielo. Herrera B, J. (2013). In vitro Translocation Cytoplasm/nucleus of Embryonic Transcription Factor OCT-4 in Perivascular Cells Suggests that Aorta as a Niche of Quiescent Adult Stem Cells. International Journal of Morphology, 1430-1438. HERZUMA. (2020). Programa Científico SEOM. Barcelona (España): HERZUMA instname:Universidad Antonio Nariño reponame:Repositorio Institucional UAN repourl:https://repositorio.uan.edu.co/ |
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spa |
| language |
spa |
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Acceso abierto |
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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) |
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https://creativecommons.org/licenses/by-nc-nd/4.0/ |
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info:eu-repo/semantics/openAccess |
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http://purl.org/coar/access_right/c_abf2 |
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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) Acceso abierto https://creativecommons.org/licenses/by-nc-nd/4.0/ http://purl.org/coar/access_right/c_abf2 |
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openAccess |
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Neiva (Huila, Colombia) |
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Universidad Antonio Nariño |
| dc.publisher.program.spa.fl_str_mv |
Odontología |
| dc.publisher.faculty.spa.fl_str_mv |
Facultad de Odontología |
| dc.publisher.campus.spa.fl_str_mv |
Neiva Buganviles |
| institution |
Universidad Antonio Nariño |
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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)Acceso abiertohttps://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_abf2García Florez, ManuelGarcía Rojas, Claudia LorenaBustos Pérez, Diego AndrésTrujillo Reyes, Karen Daniela2057162990720571626563Neiva (Huila, Colombia)2022-09-28T23:55:34Z2022-09-28T23:55:34Z2022-05-25http://repositorio.uan.edu.co/handle/123456789/6977Acevedo, P. (2015). Stem cells: general aspects, biological and molecular events. Medellin: Scielo.Aghajani, F. (2018). Evaluation of immunophenotyping, proliferation and osteogenic differentiation potential of SSEA-4 positive stem cells derived from pulp of deciduous teeth. Teheran (Iran): pubmed.Alcalá P, D. (2014). Células madre cancerígenas: conceptos actuales. México D.F.: Medigraphic.Badcock, P. (2014). The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. New yor.Fernández, A. (2008). Regeneration of the ocular surface: stem cells and reconstructive techniques. Pamplona (España): scielo.Grubelnik, G., Bostjan, E., & Pavlic, A. (2020). NANOG expression in human development and cancerogenesis. Zagreb (Croacia): Pubmed.Hernán, R., & Dorticós B, E. (2004). Medicina regenerativa. Células madre embrionarias y adultas. Habana (Cuba): Scielo.Herrera B, J. (2013). In vitro Translocation Cytoplasm/nucleus of Embryonic Transcription Factor OCT-4 in Perivascular Cells Suggests that Aorta as a Niche of Quiescent Adult Stem Cells. International Journal of Morphology, 1430-1438.Herrera B, J. (2013). In vitro Translocation Cytoplasm/nucleus of Embryonic Transcription Factor OCT-4 in Perivascular Cells Suggests that Aorta as a Niche of Quiescent Adult Stem Cells. International Journal of Morphology, 1430-1438.HERZUMA. (2020). Programa Científico SEOM. Barcelona (España): HERZUMAinstname:Universidad Antonio Nariñoreponame:Repositorio Institucional UANrepourl:https://repositorio.uan.edu.co/Introduction: Oral cancer can result from a primary lesion or originate from another distant or adjacent tumor lesion leading to malignant neoplasms in the structures and tissues of the oral cavity (metastasis). Objective: To identify TRA-I-60, OCT-4, NANOG and S SEA-4 markers in cancer stem cells of patients diagnosed with oral cancer. Materials and Methods: It corresponds to an experimental type research, by Immunofluorescence method, 5 μm slices will be routinely deparaffinized and then rehydrated. antigenic recovery will be performed in buffer 10 mm citrate buffer, Ph 6.0 for 15 minutes in an electric pressure cooker. the slides will be incubated in H2O2 1% for 15 minutes, to block endogenous peroxidase. Then 3% BSA will be used for one hour to block nonspecific binding. The sections will be incubated overnight at 4°C with the corresponding primary antibody (antiTral-60, anti-OCT-4, anti-NANOG, anti-S SSE-4), at a 1:100 dilution in 1% BSA. Results: OCT 4 marker manifested with higher intensity in well-differentiated cancer, SSEA4 marker increases with tissue dedifferentiation and NANOG and TRA-1-60 markers remain constant during tumor progression. Conclusions: Of the different markers analyzed related to progenitor cells, all were found in the patient samples, indicating among them more evident marker differences.El cáncer oral puede ser el resultado de una lesión primaria u originarse desde otra lesión tumoral distante o adyacente que conduzca a neoplasias malignas en las estructuras y tejidos de la cavidad bucal (metástasis). Objetivo: Identificar marcadores TRA-I-60, OCT-4, NANOG y S SEA-4 en células madre cancerígenas de pacientes diagnosticados con cáncer oral. Materiales y Métodos: Corresponde a una investigación de tipo experimental descriptiva de corte trasnversal, por el método de Inmunofluorescencia, los cortes de 5 μm serán desparafinados de forma rutinaria y posteriormente rehidratados. se realizará la recuperación antigénica en tampón 10 mm citrato buffer, Ph 6.0 durante 15 minutos en olla de presión eléctrica. las láminas se incubarán en H2O2 1% por 15 minutos, para bloquear la peroxidasa endógena. En seguida se usará BSA 3% por una hora para bloquear la unión inespecífica. Las secciones se dejarán en incubación durante la noche a 4°c con el anticuerpo primario correspondientes (antiTral-60, anti-OCT- 4, anti NANOG, anti-S SSE-4), en una dilución 1:100 en BSA 1%. Resultados: El marcador OCT 4 se manifestó con mayor intensidad en el cáncer bien diferenciado, el marcador SSEA4 aumenta con la desdiferenciación del tejido y los marcadores NANOG y TRA-1-60 se mantienen constantes durante la progresión del tumor. Conclusiones: De los diferentes marcadores analizados relacionados con células progenitoras, todos se encontraron en las muestras de los pacientes, indicando entre ellos diferencias de marcación más evidentes. Palabras clave: Carcinoma Escamocelular, TRA-l-60, OCT-4, TRA, NANOG y S SEA-4.Odontólogo(a)PregradoPresencialInvestigaciónspaUniversidad Antonio NariñoOdontologíaFacultad de OdontologíaNeiva BuganvilesCarcinoma EscamocelularTRA-l-60OCT-4TRANANOGS SEA-4Squamous cell carcinomaTRA-l-60,OCT-4TRANANOG and S SEA-4.Identificación de Marcadores TRA-l-60, OCT-4, NANOG y S SEA-4 en Muestras de Células Madre Cancerosas de Pacientes Diagnosticados con Cáncer OralTrabajo de grado (Pregrado y/o Especialización)http://purl.org/coar/resource_type/c_7a1fhttp://purl.org/coar/version/c_970fb48d4fbd8a85EspecializadaORIGINAL2022_Diego Andrés Bustos Perez_Karen Daniela Trujillo Reyes.pdf2022_Diego Andrés Bustos Perez_Karen Daniela Trujillo Reyes.pdfapplication/pdf3395121https://repositorio.uan.edu.co/bitstreams/3dbf9877-0c45-44cd-a3d1-55f96bbf91f7/download5acbae6f0dd717d2f871004231f3b1ccMD512022_Karen Daniela Trujillo Reyes_Diego Andrés Bustos Perez_Autorización.pdf2022_Karen Daniela Trujillo Reyes_Diego Andrés Bustos Perez_Autorización.pdfapplication/pdf1255495https://repositorio.uan.edu.co/bitstreams/e45f82cc-0b1a-498c-9b80-593ef41fc91f/downloadfe2c15e55492cded034b14369331afaaMD522022_Acta_KarenTrujillo_DiegoBustos.pdf2022_Acta_KarenTrujillo_DiegoBustos.pdfapplication/pdf173375https://repositorio.uan.edu.co/bitstreams/8104c905-e69a-42c7-a08e-3b6b321229d0/download5fb6f94b28d4229d8658d21695063248MD53123456789/6977oai:repositorio.uan.edu.co:123456789/69772024-10-09 22:47:05.867https://creativecommons.org/licenses/by-nc-nd/4.0/Acceso abiertoopen.accesshttps://repositorio.uan.edu.coRepositorio Institucional UANalertas.repositorio@uan.edu.co |